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[Clinical and pharmacological studies of technical tylosin phosphate from Pharmachem in poultry diseases].

Investigations on the healing anbd prophylactic effectiveness of the technical tylosine phosphate "Pharmachim" with 50 IU/mg activity (pharmazin T-50) in some bird diseases were carried out. It was established that a dose of 20 g/kg fodder pharmazin T-50 applied 2 days pre- and 3 days post chicken Mycoplasma gallisepticum infection protects the chickens completely from respiratory mycoplasmosis. In order to attain a sure healing and prophylactic effect in hen spirohetosis control 10 g/kg fodder applied 2 days or on the 24th to 48th day after Borrelia anserina invasion are completely sufficient. It must be noted, moreover, that pharmazin T-50 application has not a negative effect on the build up of post-vaccinal immunity following the same illness. The good results obtained by pharmazin T-50 allow it to replace successfully the remaining medicinal preparations (some of which much more expensive) applied in the control of spirohetosis and respiratory mycoplasmosis of birds.

Animals↗

Application of the egg-bit technique in poultry-disease research.

Experiments were carried out to determine the usefulness of the egg-bit technique (EBT) in poultry virus research with various agents available on the market. The EBT was found satisfactory for propagating 9 strains of avian influenza virus of different antigenic groups, with only minor differences resulting when the procedure used two sources of gelatin, presence or absence of CaCl2, or commercial or specific-pathogen-free embryos.

Animals↗

[Antibiotic resistance of several Gram-negative bacteria from clinical and dissection material from the Institute for Poultry Diseases of Ludwig Maximilian University, Munich].

A survey is given on antibiograms carried out with important bacterial strains isolated from birds during 1987. As compared with the results from earlier years no significant changes could be shown, the sensitivity ranging from 42.4% to 95.9%. The genera Bordetella and Aeromonas were included for the first time.

Animals↗

Evaluation of a mixture of trimethoprim and sulphaquinoxaline for the treatment of bacterial and coccidial diseases of poultry.

Representative experiments from work undertaken to develop a synergistic mixture of trimethoprim and sulphaquinoxaline for the preventive treatment of certain poultry diseases are described. Sulphaquinoxaline in the diet for four days was shown to achieve at least an 85 per cent higher blood level than nine other sulphonamides in chicks, and the efficacies of various trimethoprim/sulphaquinoxaline regimes in the diet or in the drinking water were demonstrated against pasteurellosis, colisepticaemia and five kinds of coccidiosis. Regimes for bacterial diseases were begun one day before infection but those for coccidial diseases were begun on the same day as infection or later. Overall, a total dose of 30 mg/kg bodyweight/day (trimethoprim/sulphaquinoxaline = 1:3) controlled these seven diseases. The same treatment was also shown to control sulphaquinoxaline-resistant strains of Escherichia coli and Eimeria acervulina. Although both drinking water and food were used for drug administration, twice the inclusion rate was required in food to that in water for equivalent efficacy. The significance of different modes of expression of dosages for bacterial and coccidial diseases is explained.

Animal Feed↗

Counter immunoelectroosmophoresis in the diagnosis of infectious bursal disease of poultry.

Infectious bursal disease virus antigen was detected in the bursa of Fabricius of infected birds by the use of the counter immunoelectroosmophoresis technique. Eight suspected outbreaks were confirmed in this way and 82 out of 89 bursal samples submitted for laboratory confirmation were positive. The test was also suitable for the detection of antibody to infectious bursal disease virus in sera of infected birds. Precipitin lines were visible within 30 min as compared with 18 to 24 h with the Ouchterlony agar gel precipitation test. Counter immunoelectroosmophoresis is recommended for rapid confirmation of infectious bursal disease of poultry.

Animals↗

Preliminary study of joint disease in poultry by the analysis of synovial fluid.

Samples of synovial fluid and synovial membrane were obtained from the hock joints of several groups of broilers, including lame birds and two strains of broilers raised on different feeding regimens and given different drug treatments (carprofen or placebo). There were more significant differences between the groups on the basis of the analysis of the synovial fluid samples than the synovial membrane samples. Experimental birds fed ad libitum had the highest median red blood cell counts and median ghost cell counts of all of the groups, but there were no differences between the groups in the thickness of the synovial lining cell layer or the degree of cellular infiltrate in the synovial membrane. The synovial fluid from the broilers and lame birds fed ad libitum was more turbid, suggestive of intra-articular pathology, and the large numbers of heterophils in samples from the lame birds indicated an inflammatory arthropathy. The birds fed ad libitum which were treated with carprofen had more cells in the synovial fluid than the birds given the placebo. A large number of the samples of synovial fluid from the ad libitum-fed broilers contained blood.

Animal Husbandry↗