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Inability of sonography to detect imminent ovulation.

Sonographic visualization of the cumulus oophorus or of morphologic alterations in the wall of the dominant follicle have been reported to be reliable signs of imminent ovulation when conventional transabdominal sonography is used. To determine if transvaginal sonography could allow a more frequent and confident prediction of imminent ovulation, we prospectively monitored 22 ovulatory menstrual cycles in four women undergoing artificial insemination and in 13 normally menstruating volunteers. Scanning was done on alternate days in the periovulatory period; a 7.5-MHz transvaginal transducer was used. Despite the improved resolution obtained with transvaginal sonography, confident identification of the cumulus oophorus or of mural changes in the follicle was not possible in any of the cycles followed. No other consistent follicular characteristic predicted imminent ovulation. We conclude that confident prediction of imminent ovulation is not possible with sonographic analysis.

Adult↗

[Indirect detection of ovulation and fertilization in the dog by progesterone level testing].

In addition to already published data (Arbeiter et al., 1990) we could achieve important facts concerning the estimation of mating time of the bitch by measuring the progesterone (P4) level continuously and observing the clinical signs on a collective of 106 ambulant patients. We found that a P4-concentration of 5 ng/ml plasma and above is signaling ovulation or ova fertilisation; a sudden rise of the progesterone over 10 ng (Ovucheck), reflecting 19.2 ng/ml (Serozyme-Progesterone), turned out to be a diagnostic mark for determination of mating time. Copulation took place between the 9th and 19th day of heat. 89% of the controllable bitches (n = 79) conceive and birth occurred 61.4 days (mean) later. The litter size and the loss of dead born puppies (4.7%) was normal with regard to the pertinent races. Because of their easy handling the P4-test kits (Ovucheck; Serozyme-Progesterone) are of good use for the practitioner.

Animals↗

Prediction and detection of ovulation relationship between woman's observations of cervical mucus changes, thermic and echographic parameters of ovulation.

Every woman can perceive and interpret her fertility signs such as cyclic changes of cervical mucus, sensation of wetness and lubrification of the vulva and thermal shift. 18 cycles were studied in 6 volunteers but one cycle was eliminated because ovulation did not occur. Each of the 6 women perceived the day in which cervical mucus was more abundant, clear, lubrificative and elastic and noted the "Peak" mucus day. These two data were correlated with the thermic nadir day and the day or the period of echographic ovulation showing an accurate relationship with ovulation.

Adult↗

[Detection of ovulation by assay of the luteinizing hormone in the urine of adolescents, before prescribing oral contraceptives].

Prior to the prescription of oral contraceptives to a series of 64 adolescent girls (mean age 18.3 years), the authors determined the presence of ovulation by measuring the basal body temperature (BBT) and the luteinising hormone (LH) in the urine. 34% of these girls had anovulatory cycles due to intermittent hyperprolactinaemia, a polycystic ovary syndrome or a pituitary microadenoma. This method should be widely used because of the obvious importance of determining the presence of ovulation prior to the prescription of oral contraceptives in order to avoid harmful effects on the hypothalamo-hypophyso-ovarian axis.

Adolescent↗

Detection of ovulation in goats by blood prostaglandins concentrations.

Eighteen mature female goats of nondescript breed were randomly assigned equally to 3 treatment groups. Group I goats served as controls, group II goats were treated (IV) with a single dose of 300 micrograms of synthetic luteinizing hormone-releasing hormone (LHRH); group III goats were treated with LHRH (similar to group II) and 24 hours later were given indomethacin (10 mg/kg; IM) which dosing was repeated every 6 hours for the next 90 hours. Blood samples were collected from all animals every 6 hours until 120 hours and assayed for prostaglandin E (PGE) and PGF2 alpha. On days 7 and 8, the animals were surgically laparotomized to observe the ovarian activity. In groups I and III goats, there was no evidence of follicular development or ovulation, but in 4 of the 6 group II goats, there were ovulatory points or corpus luteum. Mean plasma PGE and PGF2 alpha concentrations in control goats did not vary significantly (P less than or equal to 0.05) during the sampling period; but in the LHRH-treated goats (group II), these concentrations peaked twice--a low peak at 24 hours and a sharp peak at 78 to 90 hours. In contrast, these concentrations in group III goats (LHRH plus indomethacin) dropped sharply in the control group (I) value after reaching the 1st peak, and then stayed at this low plateau for the remainder of the sampling period; the 2nd peak was absent. The results indicate that the ovulation could be induced by LHRH injection in anestrus goats and that ovulation is preceded by a marked increase of plasma PGE and PGF2 alpha concentrations.

Animals↗

The detection of ovulation by intravaginal telemetry.

Following ovulatory control cycles in four celibate, healthy women, 27 to 34 years of age, basal body temperatures (BBT) were obtained in three cycles, utilizing a transistorized transvaginal telemeter. The electronic signals were collected on an automatically activated cassette receiver and later translated into temperature readings. The results were compared with values obtained simultaneously with a standard metabolic oral thermometer. The vaginal temperatures were consistently lower than the oral readings and offered a more sensitive and reproducible method of obtaining BBT. However, this system is very complex, requiring well-informed, highly motivated patients, and should be viewed currently as an investigative tool.

Body Temperature↗

Detection of ovulation by a method of change in finger-finger electropotential readings.

The purpose of this study was to determine whether ovulation can be detected by using a device (OvultronR) that measures electropotential differences in finger-to-finger contact. Daily basal body temperatures and five consecutive readings on the OvultronR were obtained each morning in 34 women for a three-month period. During one cycle an endometrial biopsy was taken and dated to document the time of ovulation. Our results showed that in only five out of 104 cycles did the device show an isolated change at the time of ovulation. This device is not a reliable method either for prediction of or the detection of ovulation. Therefore, one cannot effectively use this device alone as a basis for a rhythm method of contraception.

Adult↗

Ovulation prediction and detection with the CUE Ovulation Predictor.

Predicting ovulation is useful for managing the infertile patient and when done sufficiently in advance for fertility regulation by periodic abstinence. It has been reported that ovulation could be predicted by measurement of salivary and vaginal electrical resistance (SR and VR). These data from 32 menstrual cycles of 23 women were obtained to evaluate this technique. Data of 14 cycles were from subjects receiving clomiphene citrate (CC), while the others were from spontaneously ovulating subjects. A peak in SR was seen 5-7 days before the day of the LH peak in both natural and CC cycles. A correlation coefficient (r) of 0.94 was seen for the day of the SR peak and that of the LH peak. The SR trend was similar in both natural and CC cycles. VR in spontaneous cycles declined to a periovulatory nadir and then increased. The patterns of VR in CC cycles were similar except that values were initially depressed during and shortly after the end of CC therapy. Over 90% of VR increases were on the day of the LH peak and the day following. Results indicate that the method is useful for predicting ovulation in natural and CC cycles. Since the number of days by which ovulation is predicted exceeds the expected lifespan of spermatozoa in the female reproductive tract, the method also shows potential for use in natural family planning.

Electric Conductivity↗

Determination of urinary luteinizing hormone for prediction of ovulation.

Detection of the approximate time of ovulation is important in the in vitro fertilization-embryo transfer programs in order to avoid a possible early surge of luteinizing hormone (LH) before human chorionic gonadotropin administration in the induction of ovulation and for timing of artificial insemination. In the present study correlation between the detection of LH in urine and serum levels (mean +/- SEM) of estradiol greater than 557 +/- 118 pg/ml, progesterone less than 1.9 +/- 0.37 ng/ml and leading follicle diameter greater than 26.2 +/- 1.3 mm (mean +/- SEM) was found. Thus, the prediction of ovulation via detection of urinary LH in urine by a dipstick procedure is a good semiquantitative method, accurate enough, reliable, cheap and acceptable by the women.

Clomiphene↗

Oestradiol-17 beta and progesterone level changes in peritoneal fluid around the time of ovulation.

In 15 women, peritoneal fluid was obtained by either culdocentesis (n = 20) or laparoscopy (n = 3), before (n = 9) and after (n = 14) ovulation. Ultrasound was used for ovulation detection. Before ovulation the mean oestradiol-17 beta level in plasma and peritoneal fluid was not essentially different; the mean progesterone level was significantly higher in peritoneal fluid. After ovulation both the mean oestradiol-17 beta and progesterone levels were significantly higher in peritoneal fluid than in plasma. In peritoneal fluid, there was a wide distribution of individual oestradiol-17 beta values before and after ovulation; no significant difference existed between the mean pre- and postovulatory oestradiol-17 beta level. More consistent changes were seen in peritoneal fluid progesterone levels; the mean level was significantly higher after ovulation. In three subjects, a low postovulatory progesterone level in peritoneal fluid was associated with a cystic luteal structure observed by ultrasound, suggesting a reduced leakage of fluid from the ovulation stigma.

Ascitic Fluid↗

Application of penicillinase linked ELISA of pregnanediol glucuronide for detection of ovulation and assessment of corpus luteal function.

A penicillinase linked enzyme immunoassay was developed for the estimation of pregnanediol-3 alpha-glucuronide (PdG) in urine. The immunoassay satisfied all the validity criteria and was used in detecting ovulation and in the assessment of corpus luteal function (CLF) during spontaneous or induced cycles. Reference values were established by estimating PdG levels in daily early morning urine samples during 31 menstrual cycles obtained from 17 regularly menstruating women. A PdG value of 1.7 micrograms/mg creatinine (micrograms/mgC) (90th Centile of follicular phase) in any MLP (mid-luteal phase) sample was considered as indicating ovulation. A value of 4.6 micrograms/mgC (20th centile of MLP) was considered to be evidence of sufficient CLF. When this approach was applied to 20 infertile cases, detection of the occurrence of ovulation/anovulation was made correctly in 19 out of 20 cases (95%). Accuracy was poor (55.6%) when the aim of the diagnosis was corpus luteal deficiency. Higher accuracy (88.9%) for corpus luteal deficiency/corpus luteal adequacy was obtained when the sum of PdG concentrations in three MLP samples were taken into consideration. A total of 13.8 micrograms/mgC (thrice the 20th centile for MLP) indicated probable corpus luteal deficiency, and values above this limit were considered to indicate corpus luteal adequacy.

Adolescent↗