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PubMed1991

Distribution of cholecystokinin-like immunoreactivity within the stomatogastric nervous systems of four species of decapod crustacea.

The distribution of cholecystokinin-like immunoreactivity was studied in the stomatogastric nervous systems, pericardial organs, and haemolymph of four species of decapod crustacea, by using immunocytochemical and radioimmunoassay techniques. Whereas cholecystokinin-like immunoreactivity was found within the stomatogastric nervous systems of all four species, its distribution in each is unique. Two species (Panulirus interruptus and Homarus americanus) have cholecystokinin-like immunoreactivity within fibers and neuropil of the stomatogastric ganglion (STG); two other species (Cancer antenarius and Procambarus clarkii) do not. Further, the cholecystokinin-like immunoreactivity within the STGs of Panulirus and Homarus arise from distinct structures; from a projection of anterior ganglia in Panulirus, and from somata within the posterior motor nerves in Homarus. The staining in the other ganglia of the stomatogastric nervous system also shows some interspecies variability, although it appears to be more highly conserved than staining within the STG. These differences in staining were confirmed by measuring the amount of CCK-like peptide present in tissue extracts of ganglia by radioimmunoassay. In contrast to the variable staining within the STG, all four species have cholecystokinin-like immunoreactivity within the neurosecretory pericardial organs and thoracic segmental nerves. This cholecystokinin-like immunoreactivity is contained within fibers and within varicosities that coat the surface of these structures. The location of this staining and the presence of detectible levels of CCK-like peptide in the haemolymph suggests that CCK-like peptides in decapod crustacea may be utilized as neurohormones.

Animals↗
PubMed1991

SCPB-and FMRFamide-like immunoreactivities in lobster neurons: colocalization of distinct peptides or colabeling of the same peptide(s)?

Virtually all of the SCPB-like immunoreactive neurons (ca. 60 cells) in the lobster Homarus americanus also contain FMRFamide-like immunoreactivity. Control experiments reveal that SCPB-and FMRFamide-like immunoreactivities are successfully preadsorbed with their specific antigens, while the normal staining pattern is retained following preadsorption of each antibody with the alternate peptide. These experiments potentially lead to the conclusion that the anti-SCPB and anti-FMRFamide antibodies are labeling distinct compounds that are colocalized in lobster neurons. The lobster nervous system does not, however, contain authentic FMRFamide, but rather several FMRFamide-like compounds (Trimmer et al., J. Comp. Neurol. 266:16-26, 1987). The most abundant of these is the octapeptide TNRNFLRFamide. Experiments demonstrate that SCPB-like immunoreactivity is completely preadsorbed with synthetic TNRNFLRFamide, while there is a significant or complete loss of staining after preadsorption of the FMRFamide antibody with this molecule. Met-enkephalin-Arg-Phe-amide (YGGFMRFamide), an extended opioid peptide containing the FMRFamide sequence, also preadsorbs SCPB- and FMRFamide-like immunoreactivities, while Met-enkephalin-Arg-Phe (YGGFMRF) has no effect on the staining properties of these antibodies. These results suggest that the SCPB antibody can bind to extended forms of FMRFamide-like molecules, and that anti-SCPB and anti-FMRFamide antibodies may be colabeling one or more FMRFamide-like molecules in lobster neurons.

Amino Acid Sequence↗
PubMed1992

Antennular projections to the midbrain of the spiny lobster. I. Sensory innervation of the lateral and medial antennular neuropils.

The organization of sensory afferents in the antennular nerve (AN) of the spiny lobster and the central arborization of the afferents in the lateral and medial antennular neuropils (LAN, MAN) were analyzed by backfilling the AN with biocytin. The MAN receives primarily thick afferents (diameter greater than or equal to 10 microns) with a consistent pattern of arborization from the medial of the three major divisions of the AN. The LAN, in contrast, receives many thin to medium-sized afferents (diameter less than or equal to 0.3-5 microns), in addition some with diameters greater than or equal to 5 microns, from the lateral and dorsal divisions of the AN. In contrast to the consistent pattern of arborization in the MAN, afferents projecting to the LAN arborize in widely different patterns. Serially arranged, orthogonal side branches that are suggestive of topographical representation of the serially arranged sensilla on the antennule contribute to the stratification of the LAN. Together with existing electrophysiological data, these morphological findings are consistent with the idea that the MAN receives primarily mechanosensory (largely statocyst) input, as previously thought, but that the LAN receives chemosensory as well as mechanosensory input. The chemosensory input to the LAN would represent a novel pathway for processing chemosensory input from the antennule.

Afferent Pathways↗
PubMed1992

Antennular projections to the midbrain of the spiny lobster. II. Sensory innervation of the olfactory lobe.

The projection pattern of antennular sensory afferents in the olfactory lobe (OL) of the spiny lobster, Panulirus argus, was examined by backfilling axons in the antennular nerve (AN) with biocytin. Thin, presumptive olfactory afferents from the lateral division of the AN form a tract in the brain that diverges into a dense plexus that completely envelops the glomerular cortex of the OL. Most of the thin (diameter less than or equal to 0.3-1 microns) afferents project to single glomeruli. About 10% of the thin afferents, however, branch in the plexus and project to multiple glomeruli. A smaller number of medium-sized to thick (diameter 2-10 microns), presumably mechanosensory, afferents also innervate the OL and co-project to multiple glomeruli with the thin afferents. Afferents arborize profusely within the columnar glomeruli into very fine processes that penetrate to the base of the columns, but selectively terminate in either the cap/subcap region or in the innermost part of the base of the columns, often with conspicuous terminal boutons, forming two distinct regions of presumptive synaptic output. These results suggest that 1) The majority of the OL innervation is provided by olfactory sensilla (aesthetascs), but that other types of sensilla provide additional, likely mechanosensory, input to the OL. 2) The projection of olfactory afferents is not strictly uniglomerular. 3) The columnar organization of crustacean olfactory glomeruli is functionally significant and may provide an evolutionary correlate of the recently proposed subdivision of the vertebrate olfactory bulb into "functional columns."

Animals↗
PubMed1993

Mapping of octopamine-immunoreactive neurons in the central nervous system of the lobster.

It has been suggested that serotonin and octopamine serve important roles in behavioral regulation in lobsters. In this paper the locations of octopamine-immunoreactive neurons were mapped in wholemount preparations of the ventral nerve cord of 4th stage lobster (Homarus americanus) larvae. Approximately 86 neurons were found, distributed as follows: brain, 12; circumesophageal ganglia, 2; subesophageal ganglion, 38; thoracic ganglia, 6 each; and 4th and 5th abdominal ganglia, 2 each. All the octopamine-immunoreactive neurons are paired and located along the midline. Of the 86 neurons, 28 were identified as neurosecretory, and 26 as intersegmental ascending thoracic, ascending abdominal, or descending interneurons. The neurosecretory system is arranged segmentally and located entirely within the thoracic and subesophageal neuromeres with extensive terminal fields of endings along 2nd thoracic and subesophageal nerve roots. This set of neurons shares the features of central and peripheral endings with 2 pairs of large serotonin-containing neurosecretory neurons found in the fifth thoracic and first abdominal ganglia. The intersegmental neurons include: (1) two cells in the brain and 2 pairs of cells in the 3rd and 4th neuromeres of the subesophageal ganglion, which project to the 6th abdominal ganglion; (2) a segmentally organized group of ascending interneurons found in the subesophageal and in all thoracic ganglia; and (3) pairs of ascending interneurons found in the 4th and 5th ganglia in the abdominal nerve cord. By means of a biochemical assay, the cell bodies of octopamine-immunoreactive neurosecretory cells in the thoracic segment of the nerve cord were found to contain 40-100 fmol of octopamine, while control neurons had none.

Animals↗
PubMed1993

Comparative brain ontogeny of the crayfish and clawed lobster: implications of direct and larval development.

The freshwater crayfish Cherax destructor and the lobster Homarus americanus have many similarities including life style, body form, and neural organization. However, the ontogenic history is very different in the two species. The development of Cherax is short and direct whereas the development of Homarus comprises three pelagic larval stages and takes more than twice as long from extrusion to benthic stages at constant temperature. In order to determine the progression of maturation of the nervous system in each species and the potential implications of pelagic forms on brain structure, the timing of appearance of 22 general and neural developmental events clearly identifiable in both species was compared. The onset of serotonin antigenicity in the different parts of the brain was chosen as one marker of neural development. During the first month of embryogenesis the timing of morphological, physiological, and neural events is similar in the two species. Morphological development is then accelerated in the crayfish near hatching time and over the two postembryonic stages before the advent of the independent benthic stage. Such heterochronic processes can at least partly account for the different developmental patterns in the two decapods. Among the characters showing similar timing in the two species is the formation of glomeruli (presumptive zones of synaptic contact) in the olfactory lobes of the deutocerebrum, although this event is embryonic in Homarus but postembryonic in Cherax. In contrast, glomerular formation in the accessory lobes is heterochronic: in both species, the glomeruli of the accessory lobes are acquired postembryonically, that is, 3 to 4 months earlier in Cherax than in Homarus. These data suggest that the development of the glomeruli in the olfactory lobes may depend primarily on internal developmental signals, whereas the triggering of glomerular formation in the accessory lobes may depend on external cues. The fact that, in Homarus, only the postlarval stages show mature accessory glomeruli may be a reflection of the functional requirements of benthic life.

Animals↗
PubMed1993

Antennular projections to the midbrain of the spiny lobster. III. Central arborizations of motoneurons.

The central organization of antennular motoneurons in the brain of the spiny lobster, Panulirus argus, was analyzed by combining biocytin backfills with serial reconstructions of the antennular nerves and the brain. Eighty-nine to 99 antennular motoneurons occur in each hemibrain. The somata of the motoneurons are distributed in a consistent pattern in two complex soma clusters, the ventral paired mediolateral cluster of the deutocerebrum and the dorsal unpaired median cluster of the tritocerebrum. The motoneurons arborize ipsilaterally in the lateral and median antennular neuropils and the tegumentary neuropil. The backfills indicate a minimum of five morphological types of motoneurons with different arborization patterns. The innervation pattern of the motoneurons, together with previously reported innervation patterns of antennular sensory afferents, suggest that the lateral antennular neuropil is a lower motor center driving local antennular reflexes in response to chemical and mechanical stimulation of the antennule, whereas the median antennular neuropil is a lower motor center for equilibrium responses.

Animals↗
PubMed1994

Biotin staining in the giant fiber systems of the lobster.

The avidin-biotin-complex method is a popular immunocytochemical technique. This method labels consistently a group of neurons in the lobster ventral nerve cord in the absence of primary antibodies. The specific staining is due to a relatively high level of endogenous biotin (or biocytin) in these neurons. These biotin-positive neurons are located in the supraesophageal, thoracic, and abdominal ganglia. Intraaxonal injection of Lucifer yellow followed by Texas red-conjugated streptavidin staining reveals that the neurons are members of the medial giant (MG) and lateral giant (LG) systems, which are important in mediating rapid tail flipping during escape maneuvers. In neuronal somata, staining is restricted to the cytoplasm. Within MG axons, staining appears as punctate, subaxolemmal structures. Preincubating nerve cords in biocytin or direct intraaxonal injection of biocytin enhances staining of these punctate organelles. In LG axons, staining is localized to fragments of braided filamentous structures that also appear to be associated with the axolemma. Preincubation of ventral nerve cords in various concentrations of biocytin results in the appearance of additional groups of stained neurons, suggesting that there are subsets of neurons with specific biocytin-uptake or -retention mechanisms. In the crayfish, biotin-positive staining is confined to the MG neurons; the LG neurons are not stained. In the earthworm, no staining is observed in the MG and LG axon escape systems. In the goldfish, no biotin-staining is seen in the Mauthner neurons and their axons. The significance of specific localization of biotin or biocytin to subsets of neurons is unclear. It may reflect the presence of high levels of biocytin moieties on biotin-dependent enzymes. Biotin is an important cofactor in the catalytic functions of several decarboxylases crucial in energy production and lipogenesis. Axons of the giant fiber systems in lobsters and crayfish may have high energy and fatty acid synthesis requirements. Increased levels of biotin accumulation may also be related to other functions of the giant axon systems, such as the formation of electrical synapses among themselves and with phasic motoneurons.

Animals↗
PubMed1994

Dopamine in the lobster Homarus gammarus. I. Comparative analysis of dopamine and tyrosine hydroxylase immunoreactivities in the nervous system of the juvenile.

As a catecholamine, dopamine belongs to a class of molecules that have multiple transmitter and hormonal functions in vertebrate and invertebrate nervous systems. However, in the lobster, where many central neurons have been identified and the peripheral innervation pattern is well known, the distribution of dopamine-containing neurons has not been examined in detail. Therefore, immunocytochemical methods were used to identify neurons likely to contain dopamine and tyrosine hydroxylase in the central nervous system of the juvenile lobster Homarus gammarus. Approximately 100 neuronal somata stain for the catecholamine and/or its synthetic enzyme in the brain and ventral nerve cord. The systems of neurons labeled with dopamine and tyrosine hydroxylase antibodies have the following characteristics: 1) the two systems are nearly identical; 2) every segmental ganglion contains at least one pair of labeled neurons; 3) the positions and numbers of cell bodies labeled with each antiserum are similar in the various segmental ganglia; 4) six labeled neurons are anatomically identified; two interneurons from the brain project within the ventral cord to reach the last abdominal ganglion, two neurons from the commissural ganglia are presumably neurosecretory neurons, and two anterior unpaired medial abdominal neurons project to the hindgut muscles; and 5) no cell bodies are labeled in the stomatogastric ganglion, but fibers and terminals in the neuropil are stained. The remarkably small numbers of labeled neurons and the presence of very large labeled somata with far-reaching projections are distinctive features consistent with other modulatory aminergic systems in both vertebrates and invertebrates.

Animals↗
PubMed1994

Early embryonic expression of a 60-kD glycoprotein in the developing nervous system of the lobster.

To investigate the developmental processes that generate the crustacean nervous system, we used a monoclonal antibody that recognises an antigen that is expressed in the developing embryonic nervous system of the lobster, Homarus gammarus. Expression of this antigen commences early in embryogenesis, occurs in all parts of the embryonic central and peripheral nervous systems, and continues into adulthood. Initial expression in the central nervous system correlates with the onset of neuronal process outgrowth. Light microscopic analysis shows that the antigen is found surrounding the cell bodies and processes of all neurons. Biochemical analysis indicates that the antigen is a glycoprotein with an apparent molecular weight of 60 kD. Due to the early embryonic onset of its expression, this antigen is a useful cellular label for visualisation of pattern formation in the developing nervous system; this is documented in detail for the developing stomatogastric nervous system. The fact that the 60-kD antigen is expressed early in embryogenesis throughout the nervous system suggests that it might play an important role in the development of the lobster nervous system.

Animals↗
PubMed1994

Replacement of an inherited stretch receptor by a newly evolved stretch receptor in hippid sand crabs.

Primary sensory neurons that are motoneuron-like in morphology and often nonspiking (transmit afferent signals as graded depolarizations) characterize an unusual type of stretch receptor in decapod crustaceans. Nonspiking and spiking receptors occur in similar positions at homologous joints in different species and have been presumed to be homologous, the spiking one considered "primitive". To better understand the evolutionary origin of these stretch receptors and why some are nonspiking, we examined the spiking telson-uropod stretch receptors in the spiny sand crab Blepharipoda occidentalis (Albuneidae) and the squat lobster Munida quadrispina (Galatheidae) and compared them with the nonspking telson-uropod stretch receptor of the mole sand crab Emerita analoga (Hippidae). The position, morphology and responses to stretch of the sensory neurons, and the ultrastructure of the elastic strand portion of the receptor are similar in M. quadrispina and B. occidentalis, except that in B. occidentalis the receptor muscles are substantially smaller and the extracellular matrix of the elastic receptor strand is both more extensive and more organized, reminiscent of the ultrastructure of E. analoga's nonspiking receptor. We conclude that the spiking telson-uropod stretch receptors of albuneids and galatheids are homologous. The differences in the ultrastructure of their receptor strands imply that the efficiency of coupling receptor length change to deformation of the dendritic termini increases in the order M. quadrispina < B. occidentalis < E. analoga. The spiking and nonspiking telson-uropod stretch receptors differ anatomically in three major respects that appear to preclude their homology. (1) The receptor strands are on opposite sides of a conserved muscle. (2) The sensory somata are in different regions of the sixth abdominal ganglion: a lateral cluster of somata for the spiking sensory neurons and two medial clusters, one anterior, one posterior, for the nonspiking sensory neurons. (3) The neuropil projections of the sensory neurons are different. We conclude that the hippid's nonspiking telson-uropod stretch receptor evolved de novo and not by modification of the ancestral anomuran telson-uropod stretch receptor (which Hippidae have lost).

Action Potentials↗
PubMed1995

Development of the olfactory and accessory lobes in the American lobster: an allometric analysis and its implications for the deutocerebral structure of decapods.

The allometric changes characterizing the growth of the deutocerebrum (midbrain) of the American lobster (Homarus americanus) are studied using computerized three-dimensional reconstructions of serial brain sections. During the embryogenesis of the midbrain, the paired accessory lobes (higher order processing areas) appear later than the paired olfactory lobes (primary olfactory centers), but the former grow faster from their emergence until metamorphosis. The accessory lobes, as they enlarge, shift progressively from a medial to a posterior position in the lateral deutocerebrum. In early juvenile stages the accessory lobes are one of the largest neuropils of the brain. However, these lobes stop growing in adult animals, whereas the brain and olfactory lobes continue to enlarge, albeit at a slow rate. The overall shape of the brain and the relative proportions and locations of the deutocerebral neuropils and associated cell clusters of various lobster ontogenetic stages are similar to those of selected adult decapods. In addition, the relation between deutocerebral organization and brain size seem parallel during lobster development and across crustacean species. Measurements of the brains of 13 species of decapods (illustrated in Sandeman et al. [1993] J. Exp. Zool. 265:112, plus Homarus) indicate the following trends: Small brains possess olfactory lobes but no accessory lobes, larger brains possess accessory lobes that are medial and small relative to the olfactory lobes, and the largest brains contain relatively voluminous posterior accessory lobes. These observations indicate that some differences in the organization of the deutocerebrum are related to absolute brain size in crustaceans and suggest that ontogenetic scaling of proportions may apply to the deutocerebral neuropils of decapods. Peramorphosis and paedomorphosis in the evolution of the decapod brain are considered.

Animals↗
PubMed1995

Dopamine in the lobster Homarus gammarus: II. Dopamine-immunoreactive neurons and development of the nervous system.

Dopamine-immunoreactive neurons were revealed in lobster embryos, larvae, and postlarvae, and staining patterns were compared to neuronal labeling in the juvenile lobster nervous system (Cournil et al. [1994] J. Comp. Neurol. 344:455-469). Dopamine immunoreactivity is first detected by midembryonic life in 35-40 neuronal somata located anteriorly in brain and subesophageal ganglion. When the lobsters assume a benthic life during the first postlarval stage, an average of 58 cell bodies are labeled. The acquisition of dopamine in lobster neurons is a protracted event spanning embryonic, larval, and postlarval life and finally reaching the full complement of roughly 100 neurons in juvenile stages. Some of the dopaminergic neurons previously identified in the mature nervous system, such as the paired Br cells, L cells, and mandibular cells, are labeled in embryos and persist throughout development. In contrast, other neurons stain transiently for dopamine during the developmental period, but, by the adult stage, these neurons are no longer immunoreactive. Such transiently labeled neurons project to the foregut, the thoracic dorsal muscles, the neurohormonal pericardial plexus, and the pericardial pouches. It is proposed that these neurons are alive and functioning in adult lobster but that dopamine levels have been abolished, providing that neurotransmitter status is a dynamic, changing process.

Animals↗
PubMed1995

Structural and functional characterization of a muscle tendon proprioceptor in lobster.

A morphological and electrophysiological study was made on a unique primary mechanosensory neuron, the anterior gastric receptor (AGR), previously shown to arise from power-stroke muscle gm1 of the gastric mill system in the lobster foregut. Ultrastructural analysis of horseradish peroxidase injected AGR demonstrated that its peripheral dendrites do not ramify in muscle but are confined strictly to the connective tissue/epidermal interface in the tendon of gm1. These terminals are rich in mitochondria and at their very endings are free of glial cell wrapping, suggesting that they are the site at which mechano-transduction occurs. Extracellular axonal recordings from an in vitro neuromuscular preparation consisting of the gm1 muscle still attached to the stomatogastric nervous system, revealed that AGR is activated by passive stretch of gm1. The response to ramp stimuli displays dynamic and static components, both of which increase with the amplitude of applied stretch, while the dynamic component is also velocity sensitive. AGR is also activated by muscle contraction here elicited either by application of exogenous acetylcholine, the excitatory neurotransmitter for gm1, or by electrical stimulation of the motoneurons (GM) themselves. Consistent with a receptor lying in-series with its muscle, therefore, the effective stimulus of AGR in vivo is probably an increase in tension exerted on the tendon during active muscle contraction. In neuromuscular preparations including the bilateral commissural ganglia, stretching gm1 reflexly activates GM motoneurons at low stimulus strengths but leads to an inactivation of GM motoneurons at high stimulus strengths. This is consistent with earlier findings that both responses can be elicited by direct electrical stimulation of AGR. The functional implications of AGR's anatomical relationship with muscle gm1, the receptor's response properties, and its central effects on motor output to gm1 are discussed. Comparison is also drawn between this first reported example of a true tendon receptor in invertebrates and muscle receptors of vertebrates.

Animals↗
PubMed1983

Immature thymocytes isolated using a sialic acid-specific lectin are unresponsive to concanavalin A.

Mouse thymocytes, which are approximately 90% immature cortical cells, are low in surface sialic acid when compared with more mature cortisone-resistant, presumably medullary, thymocytes and peripheral T lymphocytes. Thus, medullary thymocytes bind and are agglutinated by the N-acetylneuraminic acid-specific lectin, lobster agglutinin 1 (LAgl), whereas cortical thymocytes are not agglutinated by this lectin. It is demonstrated herein that mouse cortical thymocytes, purified using LAgl, do not respond to the mitogenic effects of concanavalin A (Con A). The lack of response to this lectin is not due to depletion of macrophages, since addition of macrophages does not restore the response. Populations of LAgl-negative thymocytes can be made to respond weakly to Con A by the addition of interleukin 2, but this response appears to be due to the presence of a few contaminating LAg1-binding thymocytes since it is abolished by treatment of the cells with rabbit anti-LAgl serum plus complement. Therefore highly purified cortical thymocytes not only cannot respond to Con A, but also they cannot be induced to respond by the addition of the T cell growth factor, interleukin 2. Thymocytes isolated by a single criterion, that is by virtue of their low amount of surface sialic acid, appear to be a truly immature population of thymocytes.

Animals↗
PubMed1990

Isoelectric focusing in immobilized pH gradients of phosphoglucomutase and esterases from the spiny lobster.

A method is described for detecting polymorphisms of cephalothorax and tail homogenates of 25 puerulus staged Panulirus argus in phosphoglucomutase (PGM) and esterases. Isoelectric focusing in immobilized pH gradients was used. In the pH 6.0-8.0 interval for phosphoglucomutase and in the pH 3.5-5.0 and 4.2-4.9 ranges for esterases, both enzymes appeared as polymorphic band patterns. These could be explained by one locus with 2 alleles for phosphoglucomutase and 3 loci with 2, 3 and 4 alleles for esterases. Esterases exhibit a more extensive polymorphism in immobilized pH gradients than in polyacrylamide gel electrophoresis.

Alleles↗
PubMed1992

Creosote.

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Animals↗
PubMed1989

Glucose and sulfate conjugation of phenolic compounds by the spiny lobster (Panulirus argus).

Previous work has shown that the hepatopancreas of the spiny lobster (Panulirus argus) contains a mixed-function oxidase system capable of catalyzing the monooxygenation of polycyclic aromatic hydrocarbons to highly toxic products similar to those formed by mammalian tissues. Studies were designed to determine the ability of the spiny lobster to conjugate the phenolic compounds 4-methylumbelliferone, p-nitrophenol, beta-naphthol, and 3-hydroxybenzo[a]pyrene with endogenous molecules. The hepatopancreas contained UDP-glucose (UDPG) dependent glucosyltransferase, while no activity was detected when UDP-glucuronic acid was used as the cosubstrate. Atypical Michaelis-Menten kinetics result with varying concentrations of UDPG, indicating that multiple forms of glucosyltransferase may exist in this organ. The activity was localized in the microsomal fraction, exhibited a pH optimum at 8.0-8.5, and a temperature optimum of 30 degrees C. Sulfate conjugation was found only in the cytosolic fraction of the antennal gland and used adenosine 3'-phosphate 5'-phosphosulfate (PAPS) as the sulfate donor (Km(apparent) = 9.0 +/- 4.9 microM). Hepatopancreas cytosol inhibited sulfotransferase activity. The pH optimum of antennal gland sulfotransferase was a function of the substrate and ranged from 5.5 to 7.4. Analysis of spiny lobster urine 24 hr following exposure to 3-hydroxybenzo[a]pyrene demonstrated the ability of the lobster to form both the sulfate and glucoside conjugate in vivo.

Animals↗