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At least 73 records · Page 4Linked to original sources
PubMed2003

Detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood with the EVIGENE MRSA detection kit.

A total of 200 blood cultures containing putative staphylococci were analyzed by a commercial gene probe hybridization assay (EVIGENE; Statens Serum Institut, Copenhagen, Denmark), and 18 were identified as methicillin-resistant Staphylococcus aureus (MRSA) positive. Of these, 17 were positive by PCR and 16 were positive by culture. Detailed analysis of the discrepant results showed that the EVIGENE kit allowed specific identification of MRSA in blood cultures without any of the drawbacks associated with PCR.

DNA Probes↗
PubMed2004

Evaluation of the methicillin-resistant Staphylococcus aureus (MRSA)-Screen latex agglutination test for detection of MRSA of animal origin.

Methicillin (oxacillin)-resistant staphylococci (MRS) have emerged as major clinical and epidemiological pathogens, and there have been frequent reports of MRS infections in the veterinary field. The MRSA-Screen latex agglutination test (Denka Seiken Co., Ltd., Tokyo, Japan) was compared with an oxacillin agar screen test, MIC determination, and mecA PCR assay, the "gold standard." In an analysis of 15 mecA-positive and 48 mecA-negative S. aureus animal isolates, as well as 9 mecA-positive and 147 mecA-negative, coagulase-negative staphylococcal animal isolates, the latex agglutination test surpassed the widely used oxacillin agar screen method and MIC determination, with a sensitivity and a specificity of 100%. The MRSA-Screen test is a reliable and rapid method of detecting MRS in the veterinary field.

Animals↗
PubMed2005

[Combination effect of pazufloxacin and anti-mrsa drugs against beta-lactam antibiotic induced vancomycin-resistant MRSA (BIVR)].

The in vitro combination effects of pazufloxacin (PZFX) with an anti-MRSA drug such as vancomycin (VCM), teicoplanin (TEIC), arbekacin (ABK), minocycline (MINO), rifampicin (RFP) and sulfamethoxazole-trimethoprim (ST) were investigated against 26 strains of beta-lactam antibiotic induced vancomycin-resistant MRSA (BIVR) by the checkerboard method. The additive and synergistic effects were observed with the combination of PZFX and VCM (50%, 13/26 strains), PZFX and TEIC (96%, 25/26 strains), PZFX and ABK (65%, 17/26 strains), PZFX and MINO (46%, 12/26 strains), PZFX and ST (54%, 14/26 strains). The synergistic effects were observed with the combination of PZFX and TEIC (4%, 1/26 strains), PZFX and ABK or MINO (15%, 4/26 strains). The antagonistic effects were observed with only PZFX and MINO (12%, 3/26 strains), others were all indifference.

Aminoglycosides↗
PubMed2006

Emergence of MRSA infections in horses in a veterinary hospital: strain characterisation and comparison with MRSA from humans.

Methicillin-resistant Staphylococcus aureus has become an emerging public health problem worldwide, no longer only associated with healthcare-associated infections. With the exception of some recent reports concerning infections in cats, dogs and horses, infections with MRSA in companion animals have been infrequently reported. Here we submit findings for MRSA infections in horses in a central European university hospital.

Animals↗
PubMed2006

The role of gowns in preventing nosocomial transmission of methicillin-resistant Staphylococcus aureus (MRSA): gown use in MRSA control.

During a 2-year period, a standard isolation protocol for control of methicillin-resistant Staphylococcus aureus was compared with a similar protocol in which gowns were not used but the availability of alcohol-based hand disinfectant was increased. Intervention wards had 0.10 fewer new MRSA transmissions per ward per month, compared with control wards (P was not significant).

Cross Infection↗
PubMedDate not supplied

A pilot programme of MRSA surveillance in India. (MRSA Surveillance Study Group).

This surveillance study was conducted simultaneously at three centres across India. A total of 13,610 test samples from various sites were obtained. Microbiological methods employed were similar at the three centres. Identification of S aureus was based on the recognition of the production of coagulase with positive isolates being recorded as S aureus. Both tube coagulase tests and slide coagulase test were performed. Antimicrobial susceptibility testing of the isolated strains of staphylococcus aureus and staphylococcus epidermidis to various antimicrobial discs were carried out according to standardized disk diffusion method recommended by NCCLS. Of the total 739 cultures of S aureus, 235 (32%) were found to be multiply resistant with the individual figures for resistance being 27% (Bombay), 42.5% (Delhi) and 47% (Bangalore). MRSA is emerging to be a significant problem pathogen in the surgical setting with vancomycin probably the only reliable choice for these infections.

Cross Infection↗
PubMed2006

Reduction in incidence of nosocomial methicillin-resistant Staphylococcus aureus (MRSA) infection in an intensive care unit: role of treatment with mupirocin ointment and chlorhexidine baths for nasal carriers of MRSA.

After the introduction of routine treatment for every nasal carrier of methicillin-resistant Staphylococcus aureus, active follow-up surveillance for nosocomial methicillin-resistant S. aureus infection was conducted for 5 years in an intensive care unit of a tertiary-care teaching hospital. There was a significant decrease in the incidence of nosocomial methicillin-resistant S. aureus infection during the later years of follow-up. Decolonization of nasal carriers of methicillin-resistant S. aureus is probably associated with such findings.

Anti-Bacterial Agents↗