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At least 73 records · Page 4Linked to original sources
PubMed1997

Titanium for removable dentures. I. Laboratory procedures.

To test the hypothesis that titanium (Ti) removable partial dentures (RPDs) would function for a period of at least 2 years without failure, 10 patients were selected to receive dentures made from Ti and Co-Cr. The Ti RPDs were constructed identically to conventional cobalt-chromium (Co-Cr) dentures. Five complete Ti dentures were also included and the laboratory procedures involved for making both complete and partial dentures were evaluated. The detection of internal defects by radiography made the screening of Ti castings possible and led to a rejection of specimens showing porosities of more than 0.5 mm in flexible members. Co-Cr frames on the other hand cannot be screened in this way. The success rate in the casting of Ti was 60% for both partial and complete dentures, but as casting technology has since improved, the rate is expected to be higher, particularly where complete denture palates have optimum thickness. The success rate in the casting of Co-Cr RPDs was 100% without radiographic screening. The weight difference between Ti and Co-Cr RPDs was in the range of 1.3 to 3.9 g at issue and is generally higher as the volume of denture frames increases. A large difference would be of clinical significance in maxillary complete dentures. The low density of Ti allows for the adoption of a useful pre-clinical quality-control process using commonly available dental X-ray units.

Chromium Alloys↗
PubMed1978

Computerization of clinical laboratory procedure manuals.

Current code requires federally licensed clinical laboratories to have standard operating procedure manuals describing all tests performed. A computerized system for generating, revising, and excerpting manuals, which produces hard copy and microfiche, has been developed and appears to meet the regulatory criteria. The salient aspects of pertinent federal regulations, the features of the computerized system, the format of the manual, the users' experience therewith are described.

Computers↗
PubMed1999

Quality specifications of clinical laboratory procedures: developing country needs.

In developing countries laboratory measurements are made in specimens of populations that are usually of different genetic origin and live in a different environment, under working conditions that differ from those of industrialized countries. The setting of quality specifications of laboratory measurements must take into account such differences to improve the quality of laboratory services in these countries. Industry and professional organizations should be aware of the differences, otherwise their recommendations may counteract efforts to improve laboratory services in developing countries.

Clinical Laboratory Techniques↗
PubMed1991

Occlusal accuracy in restorative dentistry: the role of the clinician in controlling clinical and laboratory procedures.

This paper describes the clinical and laboratory stages necessary to ensure the occlusal precision of fixed restorations. The important diagnostic stages, including elimination of mandibular dysfunction, the occlusal analysis and waxing of diagnostic casts, and the diagnostic use of provisional restorations, are outlined. The importance of having the clinician control all stages of laboratory work, including die trimming, mounting working casts on an articulator, the determination of both anterior and posterior tooth form, and the final occlusal adjustment of the restorations, is emphasized.

Dental Articulators↗
PubMed1993

Evaluation of sensitivity of 10 diagnostic assays for Chlamydia trachomatis by use of a simple laboratory procedure.

AIMS: To determine the sensitivity of commercially available diagnostic assays for Chlamydia trachomatis using a simple method. METHODS: Nine commercial assays and an "in-house" polymerase chain reaction (PCR) were evaluated using serial dilutions of a laboratory grown H serovar--four of them using a laboratory grown E serovar. Seven of the assays were further tested using dilutions of several cervical samples known to contain chlamydiae. RESULTS: The most sensitive assays were the MicroTrak direct fluorescent antibody (DFA) test (Syva) and the PCR which detected C trachomatis at a 10(-8) dilution of the H serovar, while the two least sensitive, Clearview (Unipath) and TestPack (Abbott), were positive only at 10(-4) and 10(-3) dilutions, respectively. A range of enzyme immunoassays (EIAs) and a nucleic acid hybridisation test were of intermediate sensitivity. The results with serovar E were consistent with these. When clinical samples were examined, the DFA test detected C trachomatis in dilutions at least 10-fold greater than any other assay. CONCLUSIONS: The range of sensitivity of diagnostic assays determined by the laboratory dilution procedure is very wide. Sensitivity assessed in this way, however, reflects the ability of the assays to detect C trachomatis in large scale clinical trials. The dilution procedure, which is simple to undertake, could therefore be applied by any laboratory before a new diagnostic method is considered for routine use.

Chlamydia Infections↗
PubMed1993

Evaluation of sensitivity of 10 diagnostic assays for Chlamydia trachomatis by use of a simple laboratory procedure.

AIMS: To determine the sensitivity of commercially available diagnostic assays for Chlamydia trachomatis using a simple method. METHODS: Nine commercial assays and an "in-house" polymerase chain reaction (PCR) were evaluated using serial dilutions of a laboratory grown H serovar--four of them using a laboratory grown E serovar. Seven of the assays were further tested using dilutions of several cervical samples known to contain chlamydiae. RESULTS: The most sensitive assays were the MicroTrak direct fluorescent antibody (DFA) test (Syva) and the PCR which detected C trachomatis at a 10(-8) dilution of the H serovar, while the two least sensitive, Clearview (Unipath) and TestPack (Abbott), were positive only at 10(-4) and-3 dilutions, respectively. A range of enzyme immunoassays (EIAs) and a nucleic acid hybridisation test were of intermediate sensitivity. The results with serovar E were consistent with these. When clinical samples were examined, the DFA test detected C trachomatis in dilutions at least 10-fold greater than any other assay. CONCLUSIONS: The range of sensitivity of diagnostic assays determined by the laboratory dilution procedure is very wide. Sensitivity assessed in this way, however, reflects the ability of the assays to detect C trachomatis in large scale clinical trials. The dilution procedure, which is simple to undertake, could therefore be applied by any laboratory before a new diagnostic method is considered for routine use.

Bacteriological Techniques↗
PubMedDate not supplied

[A restraining device for rats for laboratory procedures].

The difficulty in handling not anaesthetized rats for laboratory purposes led the author to develop a specially made device, with two distinct parts: a wood base and a retainer. The latter is made with plastic tubes used in buildings, and holds effectively and harmless the animal, allowing manipulations and applications through holes in one of its face.

Animals↗
PubMed1990

Quality control in flow cytometry for diagnostic pathology. I. Cell surface phenotyping and general laboratory procedures.

Flow cytometric analyses have become commonplace in the clinical laboratory for determining cell lineage and for quantitation of cells bearing a given phenotype. Because these assays are being conducted to support diagnoses or assist in determining therapy, it is crucial to ensure that these tests are highly accurate and reproducible within a laboratory and among laboratories involved in similar endeavors. This quality assurance has been slow evolving in clinical flow cytometry for a variety of reasons: the exquisite sensitivity and delicacy of the instrumentation that recognize previously undetectable variations in staining; the constant improvement of the hardware and software; the rapid development of new techniques and reagents of clinical interest; and the failure of any existing specialty or subspecialty to encompass all aspects of flow cytometry. This article provides an overview of quality assurance necessary for the flow cytometric analysis of cell surface markers. Practical experience, published studies, and suggested guidelines from accreditation agencies have been combined to develop the text.

Cell Separation↗
PubMedDate not supplied

Laboratory procedures for detoxification of equipment and waste contaminated with brevetoxins PbTx-2 and PbTx-3.

Procedures are presented for the detoxification of laboratory equipment contaminated with brevetoxins PbTx-2 and PbTx-3. An in vivo bioassay using the Japanese madaka Oryzias latipes monitored toxin activity. Incubation for 10 min with 0.1N NaOH detoxified a lethal dose of either PbTx-2 or PbTx-3 to levels below the detection limit of the assay (less than 10 ng/mL). Decreasing the NaOH concentration required correspondingly longer incubation times. The potency of both toxins was also destroyed by incubation for 10-15 min at 500 degrees C. Steam autoclaving 30 min at 122 degrees C (18 psi) was not sufficient to detoxify brevetoxins. These results show that laboratory equipment will be decontaminated and rendered safe for normal handling by washing or soaking it in a dilute NaOH solution. Disposable waste can be either soaked in an NaOH solution prior to disposal or burned in an incinerator with a combustion chamber of at least 500 degrees C. Steam autoclaving, however, is not a viable method of decontamination.

Animals↗