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At least 73 records · Page 4Linked to original sources

ESR/spin probe study of ice cream.

Spin probes based on the 1,1,3,3-tetramethylisoindolin-2-yl structure have been used, in conjunction with electron spin resonance spectroscopy (ESR), to study the physical changes occurring in ice cream during freezing and melting. The ESR measurements allowed the rotational correlation times, tau(B), of the spin probes to be determined. Two probes were used together in a given sample of ice cream, namely, 1,1,3,3-tetramethylisoindolin-2-yl (TMIO), which samples the fat phase, and the sodium salt of 1,1,3,3-tetramethylisoindolin-2-yloxyl-5-sulfonate (NaTMIOS), which samples the aqueous phase. Data from the TMIO probe showed that when ice cream is cooled, the fat phase is a mixture of solid and liquid fat until a temperature of approximately -60 degrees C is reached. The water-soluble probe NaTMIOS showed that the aqueous phase changes completely from liquid to solid within 1 degrees C of -18 degrees C. On cooling further to -24.7 degrees C and then allowing it to warm to +25.0 degrees C, the rotational correlation times of the NaTMIOS were slow to recover to their previous values. For the lipid phase, tau(B)(298) was found to be 65.7 +/- 2.0 ps and the corresponding activation enthalpy, DeltaH, was 32.5 +/- 0.9 kJ mol(-)(1): These values are typical of those expected to be found in the type of fat used to make ice cream. The water phase gave corresponding values of 32.2 +/- 0.5 ps and 24.5 +/- 0.4 kJ mol(-)(1) values, which are those expected for a sucrose concentration of 24%.

Chemical Phenomena↗

Effect of milk fat content on flavor perception of vanilla ice cream.

The effects of milk fat concentration on flavor perception of vanilla ice cream (with 0.5 to 10% fat) were studied by sensory analyses. The percentage of free vanillin in the ice cream was determined by HPLC. The HPLC data showed that the amount of free vanillin decreased when fat content increased. Perceptions of vanilla flavor and sweetness were evaluated by a trained panel using time-intensity methodology. No significant difference was found in sweetness perception. Among 11 time-intensity parameters for vanilla flavor perception, the panel found a significant difference only in the time required to reach maximum vanilla intensity. However, free-choice profiling and a consumer preference panel showed, respectively, that, as fat content was increased, sensory quality improved, and overall preference increased.

Animals↗

Ice-cream truck-related injuries to children.

Few studies exist on the cause and nature of injuries to children from ice-cream truck-related accidents. We reviewed the medical records of 11 children with orthopedic injuries treated at Children's Hospital-San Diego from 1985 to 1995 for injuries in such accidents. Of nine children on their way to or from an ice-cream truck who were struck by an oncoming vehicle, all were girls; eight had pelvic or lower-extremity fractures. When ice-cream trucks park on the street, they pose a danger to children, because children are drawn to them, and the trucks' large size blocks the vision of oncoming drivers.

Accidents, Traffic↗

The effect of food and ice cream on the adsorption capacity of paracetamol to high surface activated charcoal: in vitro studies.

The effect of added food mixture (as if food was present in the stomach of an intoxicated patient) or 4 different types of ice cream (added as a flavouring and lubricating agent) on the adsorption of paracetamol (acetaminophen) to 2 formulations of activated charcoal was determined in vitro and compared with results from previous investigations showing a maximum adsorption capacity to the two activated charcoal-water slurries at about 0.62-0.72 g paracetamol/g activated charcoal. Activated charcoal (Carbomix or Norit Ready-To-Use), simulated gastric (pH 1.2) or intestinal (pH 7.2) fluid, and paracetamol were mixed with either food mixture or ice cream followed by one hr incubation. The maximum adsorption capacity of paracetamol to activated charcoal was calculated using Langmuirs adsorption isotherm. Paracetamol concentration was analyzed using high pressure liquid chromatography. In the presence of food, the paracetamol adsorption capacity of the 2 activated charcoals was reduced by max. 19% (P<0.05) for Carbomix(R) and by max. 11% (P<0.05) for Norit Ready-to-use compared to control without food (Hoegberg et al. 2002). Depending on which type of ice cream was mixed with the charcoal, the reductions compared to control (Hoegberg et al. 2002) varied between 11% and 26%. Even though a reduction in drug adsorption to activated charcoal was observed when food mixture or ice cream was added, the remaining adsorption capacity of both types of activated charcoal theoretically was still able to provide an effective gastrointestinal decontamination.

Acetaminophen↗

[Cottage-industry and semi-industrial ice-creams. Incidence of Staphylococcus aureus and enterotoxigenicity].

Sixty seven samples of ice-creams were taken from seven different semi-industrial and small-scale shops. 43.3% of the samples were positive for the investigation of Staphylococcus aureus in 1 g; 88% showed less than 10 St. aureus in 1 g; 10.5% between 10 and 100 and 1.5% between 101 and 1,000. In 29 strains was investigated enterotoxigenicity, as well as the response to different biochemical tests and the susceptibility to antibiotics: 21% of the strains were toxigenic (4 produced enterotoxin A, 1 enterotoxin B and 1 enterotoxins A and D). There was no difference between the toxigenic and non-toxigenic strains when biochemical tests were performed (catalase test, coagulase test, thermostable nuclease production, anaerobic utilization of glucose and mannitol, gelatin and casein hydrolysis, production of hemolysins and pigments). All the strains showed susceptibility to different antibiotics. On the basis of the present study it is proposed to incorporate in article 1075 of the Argentine Food Code the limit of 100 Staphylococcus aureus in 1 g of ice creams.

Enterotoxins↗

Spectrodensitometric determination of chloramine-T in ice cream.

A spectrodensitometric method has been developed for the quantitative determination of chloramine-T (N-chloro-N-sodium-p-toluenesulfonamide) in ice cream. Chloramine-T is extracted and converted into p-toluenesulfonamide (p-TSA) followed by thin layer chromatographic separation of concentrated extracts on silica gel and quantitation of the p-TSA spots from standards and samples by direct scanning with a reflectance densitometer at 228 nm. A linear relationship was obtained between recorded peak area and concentration for 0.5--7.0 microgram p-TSA/spot. The reproducibility of the complete method was 2.87% (n = 9 determinations). The detection limit of the scanning procedure was 0.5 microgram p-TSA/spot, corresponding to a concentration of 4 mg chloramine-T/kg sample. The average recovery was 88 +/- 3% (P = 95%) for 10 ice cream samples spiked with chloramine-T at levels ranging from 10 to 55 mg/kg. The described method was used to assay 146 commercial ice cream and whipped cream samples for chloramine-T.

Chloramines↗

Reducing ice cream energy density does not condition decreased acceptance or engender compensation following repeated exposure.

OBJECTIVES: The preferences for high-fat foods are believed to be based on their sensory attributes and energy density; however less is known about how such preferences might be weakened, other than in response to deterioration in flavor or textural quality. The aim of the present study was to see whether acceptability of reduced fat/energy foods would wane as the original post-ingestive nutritional benefits are reduced when palatability remains essentially constant. DESIGN: Repeated measures, within-subjects design conducted in two counterbalanced three week trials. SETTING/SUBJECTS: Sixteen normal-weight males (mean age 25.8 +/- 1.2 y) came to our laboratory at the Hôpital Hotel Dieu in Paris to eat an afternoon snack on 13 consecutive days (excluding weekends). INTERVENTION/OUTCOME MEASURES: Intake was recorded following repeated exposure to two flavors of standard (10% fat as a percentage of total solids weight), and low (3%) fat ice cream. One group received standard vanilla or low-fat strawberry ice cream on alternate days for two consecutive weeks; these flavor associations were reversed for a second group. The two flavors were rated as equipalatable at the beginning of the experiment at all energy levels. RESULTS: Subjects consumed the same quantity of ice cream throughout the experimental period, independent of energy density or flavor. Consequently, aggregate (summed) energy intake for subjects consuming low-fat ice cream was significantly lower (by 581 kJ (139 kcal), 15.4 g fat). Food intake records for the 24 h period immediately following the test sessions revealed no compensation for fat or energy. Despite the 28% reduction in energy density for the low-fat version, acceptance for the flavors associated with the reduced-energy versions had not declined by the end of the experimental period. CONCLUSIONS: The findings suggest that acceptance of reduced-fat foods may not be critically dependent on the post-ingestive metabolic effects when the reductions in energy density are small. Further tests with more severe reductions, and perhaps over more prolonged time periods, will be necessary to determine at what level of substitution acceptance might begin to deteriorate.

Adult↗

Detection and enumeration of Salmonella enteritidis in homemade ice cream associated with an outbreak: comparison of conventional and real-time PCR methods.

Salmonellosis caused by Salmonella Enteritidis (SE) is a significant cause of foodborne illnesses in the United States. Consumption of undercooked eggs and egg-containing products has been the primary risk factor for the disease. The importance of the bacterial enumeration technique has been enormously stressed because of the quantitative risk analysis of SE in shell eggs. Traditional enumeration methods mainly depend on slow and tedious most-probable-number (MPN) methods. Therefore, specific, sensitive, and rapid methods for SE quantitation are needed to collect sufficient data for risk assessment and food safety policy development. We previously developed a real-time quantitative PCR assay for the direct detection and enumeration of SE and, in this study, applied it to naturally contaminated ice cream samples with and without enrichment. The detection limit of the real-time PCR assay was determined with artificially inoculated ice cream. When applied to the direct detection and quantification of SE in ice cream, the real-time PCR assay was as sensitive as the conventional plate count method in frequency of detection. However, populations of SE derived from real-time quantitative PCR were approximately 1 log higher than provided by MPN and CFU values obtained by conventional culture methods. The detection and enumeration of SE in naturally contaminated ice cream can be completed in 3 h by this real-time PCR method, whereas the cultural enrichment method requires 5 to 7 days. A commercial immunoassay for the specific detection of SE was also included in the study. The real-time PCR assay proved to be a valuable tool that may be useful to the food industry in monitoring its processes to improve product quality and safety.

Colony Count, Microbial↗

Physical properties of ice cream containing milk protein concentrates.

Two milk protein concentrates (MPC, 56 and 85%) were studied as substitutes for 20 and 50% of the protein content in ice cream mix. The basic mix formula had 12% fat, 11% nonfat milk solids, 15% sweetener, and 0.3% stabilizer/emulsifier blend. Protein levels remained constant, and total solids were compensated for in MPC mixes by the addition of polydextrose. Physical properties investigated included apparent viscosity, fat globule size, melting rate, shape retention, and freezing behavior using differential scanning calorimetry. Milk protein concentrate formulations had higher mix viscosity, larger amount of fat destabilization, narrower ice melting curves, and greater shape retention compared with the control. Milk protein concentrates did not offer significant modifications of ice cream physical properties on a constant protein basis when substituted for up to 50% of the protein supplied by nonfat dry milk. Milk protein concentrates may offer ice cream manufacturers an alternative source of milk solids non-fat, especially in mixes reduced in lactose or fat, where higher milk solids nonfat are needed to compensate other losses of total solids.

Calorimetry, Differential Scanning↗

Characterization of Listeria monocytogenes from an ice cream plant by serotyping and pulsed-field gel electrophoresis.

One dominating strain of serotype 1/2b was found when serotyping and pulsed-field gel electrophoresis (PFGE) patterns were used for the characterization of 41 Listeria monocytogenes isolates originating from an ice cream plant. Samples were taken from the production environment, equipment and ice cream during the years 1990-1997. Serotyping divided the isolates into two serovars, 1/2b and 4b. Three rare-cutting enzymes (ApaI, AscI and SmaI) were used in the creation of PFGE patterns. AscI resulted in the best restriction enzyme digestion patterns (REDPs) for visual comparison. Eight different AscI REDPs were obtained, whereas ApaI produced six and SmaI seven banding patterns. When one-band differences are taken into account, 12 different PFGE types were distinguished based on information obtained with all three enzymes. The dominant PFGE type was found to have persisted in the ice cream plant for seven years. Improved and precisely targeted cleaning and disinfection practices combined with structural changes making for easier cleaning of the packaging machine, resulted in eradication of L. monocytogenes from this plant.

DNA Restriction Enzymes↗

Ice-cream consumption, tendency toward overeating, and personality.

OBJECTIVE: The exploration of the mechanisms underlying the tendency toward overeating by investigating the Dutch Eating Behavior Questionnaire (DEBQ)/Revised Eating Disorders Inventory (EDI-R) disinhibition, in sequence to the milkshake-ice cream study (van Strien, Cleven, and Schippers, in press). METHOD: In hierarchical multiple regression analyses, the relative predictive power for ice-cream consumption was assessed, that is, emotional versus external versus bulimic eating using scales of the DEBQ and the EDI-R. In nonplanned stepwise multiple regression analyses, the association was assessed between these three types of eating behaviors and non-eating-related EDI-R scales. RESULTS: Emotional eating was the most important variable for ice-cream consumption. External eating was borderline significant and bulimic eating nonsignificant when emotional and external eating had been partialled out. Emotional eating was best predicted by the EDI-R scales Asceticism, Interoceptive Awareness, and Social Insecurity. DISCUSSION: Results are consistent with psychosomatic theory, which focuses on emotional eating as the result of confusion and apprehension in recognizing and accurately responding to emotional and visceral states related to hunger and satiety.

Adult↗

A birthday party, home-made ice cream, and an outbreak of Salmonella enteritidis phage type 6 infection.

An outbreak of food poisoning caused by Salmonella enteritidis phage type (PT) 6 followed a children's birthday party. Thirty of 37 (attack rate 81%) attendees were ill, with diarrhoea and fever being the most common symptoms. One child required hospital admission for rehydration. No deaths occurred. Stool specimens from 24 out of 25 people who submitted them were positive for S. enteritidis PT 6 and one was positive for S. enteritidis PT4. Epidemiological investigation indicated that home-made ice cream was the vehicle of infection. Fresh shell eggs used raw in the ice cream were the likeliest source of infection. No ice cream or eggs were available for microbiological analysis. S. enteritidis PT6 accounts for a small but increasing proportion of all salmonellas in humans and its epidemiology is thought to be similar to S. enteritidis PT4. Thus current recommendations on the use of raw eggs in cooking continue to apply.

Child↗

Sensory and social influences on ice cream consumption by males and females in a laboratory setting.

Both male and female undergraduates ate more ice cream when eating occurred in groups of three or four than when they ate alone. These subjects also ate more ice cream when offered three different flavors than when offered only one flavor of their own choosing. For women, both variety and eating in a group combined to enhance eating more than either variable did alone. For men, variety or eating in a group caused about the same enhancement of consumption but the combination of the two variables did not lead to further increases in intake. This latter result probably reflects a ceiling effect, but it could represent a true gender difference.

Adult↗

[Microbiological quality of street-vendor ice cream in Dakar].

During a multicenter study initiated by the International Network of Pasteur Institutes and Associated Institutes, microbiological quality of street-vended ice creams in Dakar was evaluated. 313 samples of ice creams from 170 street-vendors were collected and tested for common foodborne pathogens and indicator organisms. Results showed that microbiological quality of 45% of tested samples was unsatisfactory because of large populations of aerobic mesophilic organisms (36.7%), thermotolerant coliforms bacteria (21.4%) and sometimes E. coli. (10.6%). Strict pathogens as Salmonella, Shigella and Vibrio cholerae were not found. An investigation conducted among vendors showed a lack of education and training; these vendors need information about food preparation and storage practices that reduce microbiological contamination of foods.

Adolescent↗

Determination of p-toluenesulfonamide in ice cream by combination of continuous flow and liquid chromatography: summary of collaborative study.

A collaborative study of the determination of p-toluenesulfonamide (p-TSA) in ice cream by a combination of continuous flow and on-line liquid chromatography was conducted. Seven ice cream samples containing 0-6.35 mg p-TSA/kg at 4 levels (1 blank and 3 pairs of split level samples) were analyzed by 11 laboratories. For all samples analyzed, the repeatability relative standard deviation varied from 2.08 to 3.67%, whereas the reproducibility relative standard deviation ranged from 7.79 to 11.68%. The average p-TSA values for the split levels 1, 2, and 3 were 0.55, 1.02, and 4.44 mg p-TSA/kg, respectively, with mean recoveries ranging from 76 to 79% (overall recovery range for all levels, 63-101%). No false positive results were reported for the blank sample, and no interference was encountered by the presence of vanillin in samples. The method has been adopted first action by AOAC INTERNATIONAL. 993.22 p-Toluenesulfonamide Residues in Ice Cream-Combined Continuous Flow and Liquid Chromatographic Method.

Chromatography↗

[Ice cream of industrial manufacture. Incidence of Staphylococcus aureus and enterotoxigenicity].

A total of 70 samples of ice-creams, milk, milk cream and water or fruit based were obtained from 7 different industrial producers. Of these samples, 14.3% were positive for Staphylococcus aureus in 1 g: 97.2% showed less than 10 St. aureus in 1 g, 1.4% between 101-1,000 and 1.4% between 1,001-10,000. In 21 strains enterotoxigenicity, was investigated, as well as the response to different biochemical tests and the susceptibility to antibiotics: 9.5% of the strains were toxigenic (2 strains), 1 produced enterotoxin A and 1 enterotoxin B. There was no difference between toxigenic and nontoxigenic strains when the biochemical tests were performed (catalase test, coagulase test, thermostable nuclease production, anaerobic utilization of glucose and mannitol, gelatin and casein hydrolysis, production of hemolysins and pigments). All the strains showed a wide susceptibility when they were tested with different antibiotics. Our results confirm the limit introduced to the article 1078 of the Argentine Food Code for ice-creams of industrial production (100 per g) but there is no specification of the count method to be used.

Argentina↗