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Geographic variability of Escherichia coli ribotypes from animals in Idaho and Georgia.

Several genotypic methods have been developed for determining the host origin of fecal bacteria in contaminated waters. Some of these methods rely on a host origin database to identify environmental isolates. It is not well understood to what degree these host origin isolates are geographically variable (i.e., cosmopolitan or endemic). This is important because a geographically limited host origin database may or may not be universally applicable. The objective of our study was to use one genotypic method, ribotyping, to determine the geographic variability of the fecal bacterium, Escherichia coli, from one location in Idaho and three locations in Georgia for cattle (Bos taurus), horse (Equus caballus), swine (Sus scrofa), and chicken (Gallus gallus domesticus). A total of 568 fecal E. coli isolates from Kimberly, ID (125 isolates), Athens, GA (210 isolates), Brunswick, GA (102 isolates), and Tifton, GA (131 isolates), yielded 213 ribotypes. The percentage of ribotype sharing within an animal species increased with decreased distance between geographic locations for cattle and horses, but not for swine and chicken. When the E. coli ribotypes among the four host species were compared at one location, the percent of unshared ribotypes was 86, 89, 81, and 79% for Kimberly, Athens, Brunswick, and Tifton, respectively. These data suggest that there is good ribotype separation among host animal species at each location. The ability to match environmental isolates to a host origin database may depend on a large number of environmental and host origin isolates that ideally are not geographically separated.

Animals↗

Efficiency of use of imported nitrogen, phosphorus, and potassium and potential for reducing phosphorus imports on Idaho dairy farms.

Eight commercial dairies from south central Idaho were surveyed to estimate the whole-farm surpluses of N, P, and K and to investigate the possibility of reducing P excretions through dietary manipulation. Nitrogen, P, and K imports and exports were monitored in a 12-mo period, and samples from the diets, feeds, feces, urine, and manure were collected at regular farm visits. Soils from manure-amended fields were sampled in the spring and fall. In all cases, the largest import of N, P, and K to the dairy was with purchased feeds. Major nutrient export items were milk and manure and forages, in the case of a dairy with a large land base (dairy F). Whole-farm N surplus varied from 90 to 599 t/yr (91 to 222 kg/yr per cow). The efficiency of use of imported N varied from 25 to 64%, with dairy F having the greatest efficiency of imported N use. Phosphorus and K surpluses were also significant (average of 29 and 182 t/yr and 12 and 76 kg per cow per year, respectively). During the study period, dairy F was a net exporter of K. The average efficiency of use of imported P and K was 66 and 58%, respectively. Soil P levels in the 30-cm layer were above state threshold standards, most likely from overapplication of manure. Soil nitrate-N concentrations were also high, but K concentrations were within the accepted range. Average P content of the lactating cow diets at the start of the study was 0.49% and was reduced to 0.38%. The estimated reduction in imported P due to the reduced dietary P levels was from 5.7 to 61.4 t/yr per farm, or on average 12 kg per cow per year. This study demonstrated that in addition to exports with milk and manure, export of nutrients with forages produced on the farm (dairy F) is a major factor in reducing whole-farm N, P, and K surpluses.

Animal Feed↗

Molecular epidemiology of infectious hematopoietic necrosis virus reveals complex virus traffic and evolution within southern Idaho aquaculture.

Infectious hematopoietic necrosis virus (IHNV) is a rhabdovirus which infects salmon and trout and may cause disease with up to 90% mortality. In the Hagerman Valley of Idaho, IHNV is endemic or epidemic among numerous fish farms and resource mitigation hatcheries. A previous study characterizing the genetic diversity among 84 IHNV isolates at 4 virus-endemic rainbow trout farms indicated that multiple lineages of relatively high diversity co-circulated at these facilities (Troyer et al. 2000 J Gen Virol. 81:2823-2832). We tested the hypothesis that high IHNV genetic diversity and co-circulating lineages are present in aquaculture facilities throughout this region. In this study, 73 virus isolates from 14 rainbow trout farms and 3 state hatcheries in the Hagerman Valley, isolated between 1978 and 1999, were genetically characterized by sequence analysis of a 303 nucleotide region of the glycoprotein gene. Phylogenetic and epidemiological analyses showed that multiple IHNV lineages co-circulate in a complex pattern throughout private trout farms and state hatcheries in the valley. IHNV maintained within the valley appears to have evolved significantly over the 22 yr study period.

Animals↗

A seroepidemiologic survey of three pronghorn (Antilocapra americana) populations in southeastern Idaho, 1975-1977.

Sera from 104 adult and 42 fawn pronghorn antelope (Antilocapra americana) from southeastern Idaho were tested against selected livestock pathogens. The numbers positive/numbers tested (% positive) were as follows: bovine virus diarrhea - adults 2/102 (2), fawns 0/41 (0);; infectious bovine rhinotracheitis - adults 27/101 (27), fawns 9/42 (22); parainfluenza 3 - adults 79/104 (76), fawns 22/42 (52); bovine adenovirus 7 - adults 42/103 (41), fawns 20/48 (48); bovine adenovirus 3 - adults 11/32 (34), fawns 4/14 (23); Anaplasma marginale - adults 1/104 (1), fawns 1/42 (2). There were no reactors to brucellosis, bluetongue, or epizootic hemorrhagic disease; The prevalence of reactors varied considerably for different locations and for different years.

Adenoviridae↗

A serologic survey for selected infectious diseases of black bears in Idaho.

Two hundred sixty-five black bears (Ursus americanus) from northcentral Idaho were examined serologically over a five-year period for antibodies against selected infectious disease agents. The number of positive serum samples number of sera tested and percent positive for each infectious agent is: tularemia, 65/340 (19); brucellosis, 18/332 (5); toxoplasmosis, 23/303 (8); leptospirosis, 2/196 (1); trichinosis, 16/122 (13); Q-fever, 13/210 (6); St. Louis encephalitis, 3/340 (1); western equine encephalitis, 4/334 (1); Rocky Mountain Spotted Fever, 6/282 (2). Black bears may serve as an indicator for infection in other wildlife, domestic animals and humans in the area..

Animals↗

Serologic testing of badgers to monitor plague in southwestern Idaho.

Serologic testing of badgers (Taxidea taxus) was used to monitor plague (Yersinia pestis) in a Townsend ground squirrel (Spermophilus townsendi) population in 10,000 ha of the Snake River Birds of Prey Study Area, Idaho. Eighty-six percent of the 294 sera tested in 1975 and in 1976 were positive. Significantly fewer (72%) seropositives occurred in 1977. Seasonal changes in the percentage of seropositives and the decline in 1977 were probably due to the phenology of the Townsend ground squirrel and the proportion of that species in the badger's diet. Eight Townsend ground squirrels found dead had positive bacteriologic tests for plague; however, a high mortality in the ground squirrel population was not observed. Food habits and movement patterns of badgers made them ideal for documenting the geographical and temporal characteristics of the plague focus.

Animals↗

Survey for meningeal worm (Parelaphostrongylus tenuis) and ear mites in white-tailed deer from northern Idaho.

During the fall hunting season of 1990, 95 white-tailed deer (Odocoileus virginianus) heads were collected from hunters in Boundary and Bonner Counties in northern Idaho (USA), an area currently occupied by woodland caribou (Rangifer tarandus caribou). The heads were examined for adult and larval meningeal worms (Parelaphostrongylus tenuis) by physical examination of the brain surfaces, and the Baermann technique, respectively, and for ear mites by examination of ear scrapings. Meningeal worms or mites were not detected.

Animals↗

Hemorrhagic gastritis in free-living rodents in Idaho.

Between February 1992 and March 1994, four species of rodent from the Snake River Birds of Prey Area near Boise, Idaho (USA) were necropsied. Hemorrhagic gastritis was observed in 16 of 131 Townsend's ground squirrels (Spermophilus townsendii), one of 11 Ord's kangaroo rats (Dipodomys ordii) and the one Great Basin pocket mouse (Perognathus parvus) evaluated. No lesions were observed in 14 white-footed deer mice (Peromyscus maniculatus). Tissue from one Townsend's ground squirrel was negative for Helicobacter sp.-like bacteria.

Animal Nutritional Physiological Phenomena↗

Vitreous humor analysis for selected biochemical parameters from cervids in Idaho.

Vitreous humor and liver samples were collected from hunter-harvested elk (Cervus elaphus) and mule deer (Odocoileus hemionus) in Idaho (USA). Concentrations of calcium, chloride, potassium, sodium, urea nitrogen and selenium were determined and evaluated according to species, age, gender, geographic location, and time elapsed following death. Vitreous humor analysis yielded reliable biochemical information for < or = 96 hr subsequent to the death of the animal. Vitreous potassium concentration changes over time could be used to estimate the time that elapsed following death.

Animals↗

Organochlorine residues in fish, water, and sediment of American Falls Reservoir, Idaho, 1974.

Organochlorine residues of TDE, DDE, and PCBs as high as 1.96, 2.79, and 28.74 microgram/kg, respectively, have been found in sediments of American Falls Reservoir, Idaho. Residues of TDE, DDE, and dieldrin in the flesh of sport fish were as high as 52.3, 67.2, and 160.4 microgram/kg, respectively. Maximum organochlorine residue levels found in sucker taken in the commercial fishery were 1.1 mg PCBs/kg, 781.7 microgram TDE/kg, and 82.1 microgram DDE/kg.

Animals↗

Survey of anaplasmosis in elk of the Clearwater National Forest (Idaho).

Samples of blood from 27 free-roaming elk (Cervus canadensis canadensis) from the Clearwater National Forest in north central Idaho were tested by the rapid card agglutination test and complement-fixation test for the presence of antibodies against Anaplasma marginale. The serum card test and complement-fixation test gave incomplete and false-positive reactions; the plasma card test did not give any reactions. Anaplasma bodies or other blood parasites were not observed in stained smears of elk blood. Blood from 11 elk, including 2 that were serum card test-positive, did not produce clinical, hematologic, or serologic evidence of infection in 3 anaplasmosis-susceptible bovine calves.

Anaplasmosis↗

Catastrophic cattle loss to low larkspur (Delphinium nuttallianum) in Idaho.

Low larkspur (Delphinium nuttallianum) is a toxic plant found on many western US rangelands. Episodes of fatal poisoning are often related to the abundance and toxicity of the low larkspurs. This report documents the fatal intoxication of 53 cattle in a herd of 404 animals in south-central Idaho during Spring 2002. Low larkspur had a toxic alkaloid concentration of 8.26 mg/g. Several factors were involved in a loss of this magnitude, including climatic conditions ideal for low larkspur growth, poor grass growth during the spring, the high alkaloid concentration in larkspur, and the deaths of many cattle while being moved from the pasture. It is important for livestock owners and managers to recognize toxic plants growing in their area. If there is an unusual abundance of larkspur or other toxic plants, correct identification can provoke caution in grazing management.

Animals↗

A checklist of the mosquitoes of Idaho and two new state records: Ochlerotatus decticus and Ochlerotatus euedes.

This checklist of the mosquitoes of Idaho updates the list of Brothers (1971), adding 5 species. From the literature. 3, Ochlerotatus mercurator, Ochlerotatus nevadensis, and Ochlerotatus punctor, are added and 1, Ochlerotatus stimulans, is deleted. We are reporting 2 new state records, Ochlerotatus decticus and Ochlerotatus euedes, and extending their ranges westward in the United States. One new genus and 2 new subgenera are also added.

Animals↗

Using the SF-36 to determine perceived health-related quality of life in rural Idaho seniors.

The purpose of this study was to establish the perceived health-related quality of life (HRQoL) in people aged 65 and older in rural southeastern Idaho. Ninety-five people aged 65 and older completed the Short Form 36 version 2 (SF-36 v2), a valid and reliable HRQoL instrument. Subject scores were then compared to established normative values for the general U.S. adult population and specifically to normative values for people aged 65 and older. In general, the participant's HRQoL was lower than that of the general population. However, females aged 75 and older had higher physical component summary (PCS) scores than their age- or gender-matched mates, and PCS scores of all participants aged 75 and older were higher than those of their age-matched mates. Results of a regression analysis indicated that number of prescription medications taken (p = 0.004) was the only variable predictive of PCS scores. The results of this study show a decrease in the physical aspects of HRQoL of participants, signifying that HRQoL does decline with age. Results suggest that participants aged 75 and older have a higher HRQoL than suspected, which could indicate that rural residence is not an immediate indicator of decreased quality of life in the elderly.

Age Factors↗

Description and pathology of Neoechinorhynchus idahoensis n. sp. (Acanthocephala: Neoechinorhynchidae) in Catostomus columbianus from Idaho.

Neoechinorhynchus idahoensis is described from Catostomus columbianus caught in the Salmon River, Stanley Basin, Idaho. The new species is closest to Neoechinorhynchus venustus Lynch, 1936, but is distinguished from it by its smaller and variably structured eggs, anterio-dorsal trunk hump, bent and posteriorly notched proboscis receptacle, and larger proboscis, proboscis receptacle, and hooks. It is distinguished also from 2 other species of Neoechinorhynchus with proboscis hooks in middle and anterior circles about equally large and from 7 other species having lemnisci greatly unequal in length. Histopathology of host tissue showed limited host response exemplified by epithelial damage and hemorrhaging at point of proboscis attachment with subsequent macrophage and other phagocytic cell migration. The proboscis extended through the host epithelium into the submucosa with limited hemorrhaging at the point of attachment. Unorganized collagenous fibers were present. The lumen of the host intestine was obstructed, and compressed villi were present. The trunk of the worm damaged intestinal epithelium near the crypts, causing localized inflammation. The caryophyllaeid cestode Isoglaridacris calentinei Mackiewicz, 1974, was present in concurrent infections of C. columbianus.

Acanthocephala↗

Liver lead burden in hunter-killed ducks from the Coeur d'Alene River Valley of northern Idaho.

Liver lead burden was evaluated in ducks from an area in northern Idaho contaminated with mining waste and lead shot. Hunter-killed ducks with lead shot in their gizzard had a mean liver lead concentration of 15.18 +/- 2.51 micrograms/g wet-weight (N = 23) and ducks without lead shot had a mean liver lead concentration of 4.21 +/- 0.47 micrograms/g wet-weight (N = 85). The most hazardous source of lead for waterfowl in this area has been thought to be mining waste-contaminated soils and sediments. Three sediment samples had a mean concentration of 4,520 micrograms lead/g wet-weight.

Animals↗

Serologic survey of prevalence of ovine progressive pneumonia in Idaho range sheep.

Blood samples from 2,310 mature sheep in 3 Idaho range flocks were examined by agar gel immunodiffusion to determine the prevalence of ovine progressive pneumonia. The prevalence ranged from 58% for all ages combined in one flock to 90% of cull ewes in another flock. Age-specific prevalence rates increased from 16% in yearlings to 83% in ewes greater than or equal to 7 years old. Rambouillet sheep had a significantly (P less than 0.01) lower prevalence than sheep of 5 other breeds, whereas one-half Finnsheep crosses had a significantly (P less than 0.01) higher prevalence than sheep of other breeds. Within breed and age, there was no significant difference in reproductive performance between seropositive and seronegative ewes.

Animals↗

A brief history of graduate medical education in Washington, Alaska, Montana, and Idaho.

Internships and hospital-based medical education preceded by more than 40 years the beginnings of a medical school in Washington State. Just after the turn of the 20th century, a few internships were begun by hospitals in Seattle and Spokane to help with the care of their sicker patients in the tradition of Eastern teaching hospitals. In the 1920s and 1930s, the number of hospitals with internship programs grew steadily as part of a nationwide effort at hospital standardization. Experiences in developing these programs and problems with intern recruitment contributed to the beginning of the University of Washington School of Medicine after World War II. Since the 1960s, intern and resident training has progressively become a cooperative effort of the school with many hospitals and clinics in Washington, Alaska, Montana, and Idaho contributing to the development of graduate medical education in this region.

Alaska↗