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PubMed1979

Specificity of bacteriolytic enzyme II from a soil amoeba, Hartmannella glebae.

Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fold purification was obtained. It was found that 5 and 10 mM MgCl2 significantly increased the bacteriolytic activity. It is a basic protein. The cell walls of Micrococcus lysodeikticus were lysed by the enzyme, and the products of digestion were purified by Amberlite CG-120 and Sephadex G-15 chromatography to facilitate the detection of amino sugars. After reduction of the oligosaccharides with sodium borohydride and acid hydrolysis, the amino sugars were identified by paper chromatography. It was found that enzyme II cleaved the glycosidic bond between N-acetylmuramic and and N-acetylglucosamine of the peptidoglycan moiety of the cell walls. Thus, the enzyme was identified as endo-beta-N-acetylmuramidase.

Animals↗
PubMed1992

[Associations of the soil amoeba Hartmannella rhysodes with the bacterial causative agents of plague and pseudotuberculosis in an experiment].

Some aspects of relationships between soil ameba and the causative agents of plague and pseudotuberculosis, capable of forming natural associations, are considered. Ameba can phagocytose bacteria causing plague and pseudotuberculosis. Cases of the preservation of individual bacterial cells at the stationary phase and in precysts of amebae have been registered.

Animals↗
PubMedDate not supplied

[Comparative scanning electron microscopic study of Naegleria and Acanthamoeba (Hartmannella) cysts (author's transl)].

The present study was performed with the help of scanning electron microscope and refers to the external morphology of the cysts of the free-living amoebae. The amoebae belonging to the genus Naegleria form cysts whose outer layer shows no wrinkles. It can be smooth (N. fowleri) or rough (N. gruberi). The average number of pores varies between 1.2 and 7.2. Their margin can be smooth (N. fowleri) or pierced (N. gruberi). The thickness of the bordering cell wall varies between 0.4 and 1.0 micrometer and that of the pore is about 0.6 micrometer. The cysts of the genera Schizopyrenus, Tetramitus and Didascalus are very small, show no wrinkles in their cell wall and contain no pores. In contrast, cysts in the general Acanthamoeba show their outer layer superficially plugged with a lesser number of wrinkles (A. rhysodes, A. culbertsoni) or with large or deep wrinkles inside their wall (. castellanii, A. polyphaga). There are no pores observed in their cysts. Thus the distinguishing morphological features of the cysts have generated important role in the taxonomy of the amoebae of the limax-group.

Amoeba↗