On the inadequacy of white wheat flour and fish meal for prolonged growth of pigs and its amelioration with yeast or stout.
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BACKGROUND: Workers occupationally exposed to grain dust have a high prevalence of asthma. The pathogenesis of their respiratory symptoms remains obscure when sensitization to cereal allergens has not been proved. Given the ubiquity of arthropods in stored vegetable products, we have studied the allergenic potential of two very prevalent grain pests, Eurygaster and Ephestia, as a cause of occupational asthma. We have also studied the allergenic relationship between Anisakis simplex (AS) and these pests. METHODS: We selected 15 asthmatic workers exposed to cereal dust, in whom sensitization to cereal allergens was not clear. As controls, we selected a patient who suffered from anaphylaxis after the ingestion of cereals, 6 patients sensitized to different arthropods, 1 patient who suffered from asthma after inhaling fish flour contaminated with AS, and a pool of 40 asthmatic patients with different ethiologies not due to arthropods or cereals. We performed prick tests with these pests, AS, and pure and parasitized flours, bronchial challenges, specific IgE determination, and RAST inhibition. RESULTS: All of the 15 cereal-exposed workers were sensitized to Eurygaster and Ephestia. Only 2 had detectable levels of serum IgE to these pests, but 13 presented a positive prick test result and IgE to AS without problems after eating fish. Bronchial challenges were positive to Eurygaster in 7 patients and to Ephestia in 2 patients. Twelve patients had positive prick tests to parasitized flour but not to pure flour. The patient with cereal anaplylaxis presented sensitization to both flours but not to AS. In the control group, the patient who suffered from asthma after the inhalation of AS-parasitized fish flour presented a positive prick test result and high levels of specific IgE to these pests. The RAST inhibition showed cross-reactivity between Eurygaster and AS in this patient, but the inhibition was not as evident with the sera of other workers. The same tests in the group of 40 asthmatic patients were negative. CONCLUSIONS: The clinical importance of pests as a cause of baker's asthma should not be underestimated. Sensitization to Eurygaster and Ephestia could be important in the asthma episodes suffered by our patients, and the parasitized wheat was found to have a higher allergenic potential than pure cereal flour.
During the last four years, national production of fish oil has increased appreciably due to the expansion of the "reduction" industries (fish flour). Nevertheless, this production is almost totally destined to industrial use (lubricants, tannery, etc.). In contrast, some countries like Japan, Canada and Peru have been using oil from different fish species for human consumption. In view of the above, the objectives of the present work were: first, to establish the experimental conditions for obtention of a hydrogenated fish oil and second, the formulation of fish and vegetable oil mixtures for use in the food industry. The methodology followed comprised: 1) characterization of crude fish oil through physical and chemical analysis; 2) adaptation of the vegetable oil refining procedures to fish oil, and 3) development of fish oil with vegetable oils. A hydrogenated fish oil with a melting point of 36 degrees C, iodine index of 80, and lovibond color, yellow 20, red 13, was produced. Based on the results of the sensory test, the possibility of using up to 30% extension of this oil with vegetable oils for frying, and up to 50% extension for baking, without affecting acceptability of product, was established.
Dietetic bread is mainly produced for diabetics. Gluten accounts 80% of the protein of whole wheat flour. Forty per cent wheat gluten are incorporated in the dough to increase the protein content and decrease the starch in the final product. Gluten-rich foods are still of low cost as compared to others such as skim milk, soya and fish flour.
Sweet lupine Lupinus albus cv. Multolupa) flour containing 41.7% protein and 0.025% lupanine was fed for 9 months to two generations of rats (F1 and F2) at a level that provided 20% dietary protein. The diets were supplemented with 0.2% DL-methionine. The control diet provided the same dietary protein level from defatted soya-bean flour, fish meal and dried skimmed milk. The lupine diet had no effect on the general condition, mortality or behaviour of the animals. The growth rate of males fed sweet lupine was significantly higher than that of the controls. Haematological parameters and tests of liver function were normal in all treatment groups. At autopsy there were no significant changes in the weight of the heart, kidney, spleen, brain and gonads. However, the relative weight of the liver of lupine-fed rats was significantly lower than that in the controls. The histology of the liver, like that of the other organs examined, was normal. The reproduction study did not reveal any adverse effect on fertility or lactation that could be attributed to ingestion of sweet lupine. This investigation did not disclose any deleterious effects through two generations of rats that were fed sweet lupine at a level that provided 20% dietary protein for 9 months.
Fish protein concentrate (FPC) from river Nile bolti fish (Tilapia nilotica) was prepared and compared with commercial FPC and fresh bolti fish flesh. Fish meal (FM) from bolti fish offals was prepared and compared with commercial FM and also fresh bolti fish flesh. FPC from bolti fish showed a higher crude protein content but less fat, ash, calcium and sand than the commercial sample, while FM from bolti fish showed a higher content of ash and phosphorus than commercial FM but was nearly similar in crude protein, fat, calcium and sodium chloride. FPC from bolti fish had a higher content of lysine, arginine, aspartic acid, glycine and glutamic acid and a lower content of the other free amino acids. The bolti fish FM had a lower content of total amino acids and the contents of the free amino acids cysteine, glycine, aspartic acid, serine, alanine, valine, and methionine increased slightly. The yield was 12% for FPC and 19.5% for FM. Coliform bacteria were not present in both FPC and FM from bolti fish. Low moisture contents of FPC and FM were essential for preventing microbiol growth and to attain a good keeping quality. The FPC and FM from bolti fish reached moisture equilibrium and stopped increasing in weight within 144 to 192 h.
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As a contribution to the discussion on the "Verordnung uber Umweltkontaminanten" (draft) we examined 73 double samples of the frequent types of edible fish and 2 samples each of fish oil and fish meal for their content of arsenic (As), cadmium (Cd), lead (Pb) and mercury (Hg). Arsenic was determined photometrically, cadmium and lead by anodic stripping voltammetry and mercury by flameless atomic absorption spectrometry. We found the following average contents (ppm): 0.84 As, 0.003 Cd, 0.08 Pb, 0.06 Hg. The limits (ppm) set up in the draft for a German "Höchstmengenverordnung" (regulation specifying the maximum concentrations allowed) were by far not reached in the case of Cd, Pb, and Hg with all samples while they were fulfilled in the case of As by only 18% of the samples. It seems that the limit for As of 0.5 ppm has been set up too low. In the average, the following proportion applies for the trace contents found: Cd:Hg:Pb:As = 1:20:27:280. Correlations between the contents of trace metals on the one hand and fishing grounds or trawler or sample piece on the other could not be found.
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