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Microbial astronauts: assembling microbial communities for advanced life support systems.

Extension of human habitation into space requires that humans carry with them many of the microorganisms with which they coexist on Earth. The ubiquity of microorganisms in close association with all living things and biogeochemical processes on Earth predicates that they must also play a critical role in maintaining the viability of human life in space. Even though bacterial populations exist as locally adapted ecotypes, the abundance of individuals in microbial species is so large that dispersal is unlikely to be limited by geographical barriers on Earth (i.e., for most environments "everything is everywhere" given enough time). This will not be true for microbial communities in space where local species richness will be relatively low because of sterilization protocols prior to launch and physical barriers between Earth and spacecraft after launch. Although community diversity will be sufficient to sustain ecosystem function at the onset, richness and evenness may decline over time such that biological systems either lose functional potential (e.g., bioreactors may fail to reduce BOD or nitrogen load) or become susceptible to invasion by human-associated microorganisms (pathogens) over time. Research at the John F. Kennedy Space Center has evaluated fundamental properties of microbial diversity and community assembly in prototype bioregenerative systems for NASA Advanced Life Support. Successional trends related to increased niche specialization, including an apparent increase in the proportion of nonculturable types of organisms, have been consistently observed. In addition, the stability of the microbial communities, as defined by their resistance to invasion by human-associated microorganisms, has been correlated to their diversity. Overall, these results reflect the significant challenges ahead for the assembly of stable, functional communities using gnotobiotic approaches, and the need to better define the basic biological principles that define ecosystem processes in the space environment.

Bacteria↗

Survival of methanogenic archaea from Siberian permafrost under simulated Martian thermal conditions.

Methanogenic archaea from Siberian permafrost complementary to the already well-studied methanogens from non-permafrost habitats were exposed to simulated Martian conditions. After 22 days of exposure to thermo-physical conditions at Martian low- and mid-latitudes up to 90% of methanogenic archaea from Siberian permafrost survived in pure cultures as well as in environmental samples. In contrast, only 0.3%-5.8% of reference organisms from non-permafrost habitats survived at these conditions. This suggests that methanogens from terrestrial permafrost seem to be remarkably resistant to Martian conditions. Our data also suggest that in scenario of subsurface lithoautotrophic life on Mars, methanogenic archaea from Siberian permafrost could be used as appropriate candidates for the microbial life on Mars.

Archaea↗

The universe: a cryogenic habitat for microbial life.

Panspermia, an ancient idea, posits that microbial life is ubiquitous in the Universe. After several decades of almost irrational rejection, panspermia is at last coming to be regarded as a serious contender for the beginnings of life on our planet. Astronomical data is shown to be consistent with the widespread distribution of complex organic molecules and dust particles that may have a biological provenance. A minuscule (10(-21)) survival rate of freeze-dried bacteria in space is all that is needed to ensure the continual re-cycling of cosmic microbial life in the galaxy. Evidence that terrestrial life may have come from elsewhere in the solar system has accumulated over the past decade. Mars is seen by some as a possible source of terrestrial life, but some hundreds of billions of comets that enveloped the entire solar system, are a far more likely primordial reservoir of life. Comets would then have seeded Earth, Mars, and indeed all other habitable planetary bodies in the inner regions of the solar system. The implications of this point of view, which was developed in conjunction with the late Sir Fred Hoyle since the 1970s, are now becoming amenable to direct empirical test by studies of pristine organic material in the stratosphere. The ancient theory of panspermia may be on the verge of vindication, in which case the entire universe would be a grand crucible of cryomicrobiology.

Astronomical Phenomena↗

Lunar outpost agriculture.

America's long-term commitment to a new Space Exploration Initiative has focused attention on the basic requirements for establishing a permanently manned lunar outpost and, ultimately, a martian one. High among these is the development of Regenerative Life Support Systems--with lunar agriculture an essential component--to provide a high level of self-sufficiency.

Agriculture↗

Changes in the fungal autoflora of Apollo astronauts.

Specimens were repeatedly obtained for mycological examination from the skin, throat, urine, and feces of the six astronauts who conducted the Apollo 14 and Apollo 15 lunar exploration missions. Analysis of preflight data demonstrates that the process of severely restricting opportunities from colonization for 3 weeks before flight resulted in a 50% reduction in the number of isolated species. Postflight data indicate that exposure to the space flight environment for up to 2 weeks resulted in an even greater reduction with a relative increase in the potential pathogen Candida albicans. No incidences of microbial shock were observed when crewmembers were quarantined for 16 days after completion of the space flight. Intercrew transfer of particular species could not be demonstrated because most species were not consistently recovered.

Adult↗

Airtight sealing a Mars base.

Atmospheric leakage from a Mars base would create a demand for continuous or periodic replenishment, which would in turn require extraction or mining for oxygen and other gases from local resources and attendant equipment and energy requirements for such operations. It therefore becomes a high priority to minimize leakage. This article quantifies leak rates as determined by the size of holes and discusses the implications of pressure for structural configuration. The author engineered the sealing of Biosphere 2 from which comparisons are drawn.

Air Conditioning↗

[Possibility of using higher plants in a life-support system on the moon].

The paper discusses the possibility of repeated termination of plant vegetation by prolonged darkness approximating the lunar night. This may be helpful for the incorporation of higher plants into the life support system of lunar bases, the solar light being used for illumination. In this connection vegetables (beet Bordeaux, turnip Petrovskaya, carrot Chantanet, dill, radish Virovsky white) and wheat (variety Sonora) were cultivated during the lunar light-dark cycle (i. e. 15 day light: 15 day dark). The experiments demonstrated that traditional plant products can be obtained under the conditions of lunar photoperiod. Grain of wheat grown during the lunar photoperiod were tested as seed material for further cultivation under similar conditions.

Ecological Systems, Closed↗