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Human pluripotent embryonic stem (ES) cells have important potential in regenerative medicine and as models for human preimplantation development; however, debate continues over whether embryos should be destroyed to produce human ES cells. We have derived four ES cell lines on mouse embryonic fibroblast cells in medium supplemented with basic fibroblast growth factor, human recombinant leukemia inhibitory factor, and fetal bovine serum. The source of these cell lines was poor-quality embryos that in the course of routine clinical practice would have been discarded. After continuous proliferation in vitro for more than 12 months, these ES cell lines maintained their developmental potential to form trophoblast and somatic cells, including cardiac muscle and neuronal tissue.
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The author discusses the ruling by the Spanish Constitutional Court (116/99, 17 June) in the matter of the challenge on grounds of alleged inconstitutionality lodged against the Law on Assisted Reproduction Techniques back in 1989. Other circumstances concerning the application and development of said law are examined also and a detailed critical study is made of the legal points used in the Ruling. The article concludes with some thoughts on the main issues arising therefrom.