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At least 73 records · Page 4Linked to original sources
PubMed1995

Effect of the carbon dioxide laser on viability of ear cartilage in a rabbit model.

The carbon dioxide (CO2) laser can be used for rapid, detailed sculpting of cartilage for creation of an ear framework for reconstruction of microtia. Clinical and animal studies of the effect of the CO2 laser have noted good healing with little evidence of a zone of tissue injury adjacent to the laser incisions. The current study has investigated the longer term effect of CO2 on chondrocyte viability in a rabbit ear model in which the laser has been used to incise autogenous cartilage segments for implantation into subcutaneous pockets. Over a 3-month period, the conformational integrity of the segments, when compared to segments incised with a scalpel, was no different. However, radioactive sulfur uptake studies to assess the viability of chondrocytes indicated a decrease in chondrocyte density in those specimens that have been subjected to laser incisions. Although other studies of acute cellular injury indicate that the CO2 laser may be beneficial for cartilage incision and sculpting, the current study indicates that resorption over longer periods of time might be encountered as a consequence of decreased chondrocyte viability in the vicinity of the laser incisions.

Analysis of Variance↗
PubMed1980

Comparison of aortic and ear cartilage tropoelastins isolated from lathyritic pigs.

Tropoelastin was isolated from aortae and auricular cartilage obtained from lathyritic piglets. The two tissue-specific tropoelastins were judged homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration on a high-pressure liquid chromatograph. Comparative studies of the tropoelastins were performed. Amino acid analysis revealed that the aortic and cartilage tropoelastins were very similar, if not identical, with the only exception that the cartilage tropoelastin contained more hydroxyproline and less lysine residues, both of which can be attributable to post-translational modifications. Both tropoelastins possess an apparent molecular weight of 70 000 and exhibit similar peptide fragments with limited trypsin cleavage. Antiserum raised to the aortic tropoelastin was used to show immunological identity between the two tissue tropoelastins.

Amino Acid Sequence↗
PubMed2004

Otoplasty: anterior abrasion of ear cartilage with dermabrader.

BACKGROUND: The international literature describes many different techniques for correcting prominent ears. Some of these techniques use weakening of the anterior surface in an "open or close" way. Others use suturing of the unfold antihelix. Still others suture the concha with mastoid, and some combine several of these procedures. There is thus no most widely accepted single method. Since 1993, the senior author has used the described technique on a group of 40 patients (35 bilateral and 5 monolateral cases). Before 1993, the senior author always used an "open" technique, but the anterior cartilage was weakened by the partial parallel incision procedure. METHODS: In the current study, the surgical approach for cases with a normal or moderately deep concha consisted of open anterior otoplasty with cartilage abrasion by an electric burr alone. If the concha was severely hypertrophied, concha-mastoid suturing was combined with otoabrasion, as previously described. RESULTS: The follow-up period was a minimum of 16 months and a maximum of 8 years. Complications were rare and rapidly resolved. The outcome was successful whether the burr alone or a combined method was used. CONCLUSIONS: The authors believe that anterior abrasion of ear cartilage is a useful, simple technique that is not too time consuming by which an immediate postoperative aesthetic improvement is reached. It also is more precise and safe than the partial parallel incision procedure.

Adolescent↗
PubMed1976

Electron microscopic autoradiography of 35SO4-labelled material closely associated with collagen fibrils in mammalian synovium and ear cartilage.

Electron microscopic autoradiography of connective tissue obtained from mice and rabbits previously injected with 35SO4 indicated that sulphated proteoglycans are localized on collagen fibrils. Ruthenium Red-positive transverse belts surrounding fibrils near the a-bands were heavily labelled, but fine lateral filaments of Ruthenium Red-positive material were not. These filaments, which interconnect collagen fibrils in a variety of connective tissues may represent linear aggregations of hyaluronic acid, glycoproteins and non-sulphated or long-lived sulphated proteoglycans.

Animals↗
PubMed2000

In vitro ear cartilage shaping with carbon dioxide laser: an experimental study.

Deformed cartilage remodeling appears to be a challenge, as cartilage tends to keep its initial shape because of internal stresses. Cartilage can be remodeled with heat, and this report describes the use of a CO2 laser beam for in vitro cartilaginous tissue remodeling. Straight cartilage samples were removed from the ears of 21 rabbits deprived of perichondrium and remodeled with the CO2 laser at an output power of 3 W, a spot diameter of 2 mm, and an exposure time of 0.5 second. The remodeled cartilages and control samples were implanted into the rabbits' backs and retrieved 6 to 12 months later. Histologic and morphological analysis showed that the irradiated cartilages retained both their shape and their viability. This may be a useful clinical technique for in situ remodeling of a deformed cartilage in operations such as septoplasty.

Animals↗
PubMed1986

Intracellular lipids in rabbit ear cartilage during tissue regeneration.

Circular holes 10 mm in diameter were punched in the proximal region of rabbit ears, and the intracellular lipid content of the regenerated cartilage and the neighbouring old cartilage was studied histochemically up to the 32nd week after wounding. Chondroblasts appeared in the peripheral zone of the regenerated tissue towards the end of the 3rd week, but no intracellular lipids were observed until the 8th week. In the following weeks chondrocytes and intracellular lipids appeared in the intermediate and central zones of the regenerated tissue, the production of cartilage and intracellular lipid being particularly vigorous in the central zone during the last weeks of the study. In the old cartilage adjoining the wound, intracellular lipid levels declined considerably except in new cells produced by proliferation of the perichondrium after wounding. Phospholipids were only observed in the youngest cartilage cells and disappeared when deposits of neutral lipids began to be formed. The interpretation of these observations and the function of intracellular lipids in cartilage are discussed.

Animals↗