Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “EBER”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 73 records · Page 4Linked to original sources
PubMed1995

[EBER-1 expression in salivary glands of Sjögren's syndrome].

EB virus-encoded small nuclear RNAs (EBERs) which present in large numbers during persistent infection, are known to stably bind to SSB/La antigen, leading to speculation that EBV may be involved in the pathogenesis of primary Sjögren's syndrome (1 degree SjS). Although studied by many investigators serologically, immunohistochemically and molecular biologically, this remains controversial. In situ hybridization study for EBER-1 showed positive hybridization in ductal epithelial cells in nine of eighteen salivary glands (SGs). Hybridization was more intense in ducts surrounded by infiltrating mononuclear cells (MNCs). Positive hybridization was also seen in infiltrating MNCs in eight 1 degree SjS SGs. These results indicate that EBV plays some pathogenic roles in some cases of 1 degree SjS through induction of SSB/La antibody and impairment of lymphocytes.

Autoantigens↗
PubMed1996

Detection of EBER nuclear RNA in T-cell lymphomas involving the skin--an in situ hybridization study.

To examine the relationship of Epstein-Barr virus (EBV) to cutaneous T-cell lymphoma (CTCL), a retrospective study was performed using formalin-fixed paraffin-embedded skin biopsies from 12 patients with cutaneous T-cell lymphoma. All cases were immunophenotyped with a panel of monoclonal antibodies including CD45RO, CD43, MB-2, CD20, and CD68. In situ hybridization studies to detect the EBV genome were performed, using the EBV-RNA probes for the early RNAs of EBV, namely EBER and BHLF. Among the 12 patients, six had primary CTCL, and six had secondary CTCL. The nasal cavity was the most frequent extracutaneous primary site. Immunophenotypically, all of the cases were of the T-cell lineage. In situ hybridization demonstrated a diffuse strong positive reaction for EBV transcripts (EBER) nuclear RNA in five out of 12 cases (41.7%) and a negative reaction for BHLF nuclear RNA probe in all the cases studied. Angiocentric lymphomas were positive in three out of the five cases (60%) studied. Secondary CTCL had a higher correlation with EBV than did primary CTCL, especially primary nasal T-cell lymphoma with subsequent cutaneous involvement. The EBV genome in lymphoma cells existed in a latently infected form but not in an actively replicating form. Three out of the five EBV-positive patients died, 8 months to 4 years after diagnosis. EBV infection alone in the lymphoma does not seem to affect the prognosis of CTCL patients significantly.

Adolescent↗
PubMed1993

[Polytrauma and predicting fatality. Comparative evaluation of the value of the Kirkpatrick-Youmans Trauma Index (TI), Schreinlechner-Eber modified TI (TI mod.), and the Baker Injury Severity Score].

The Trauma Index (TI) of Kirkpatrick and Youmans modified by Schreinlechner and Eber performed better than the Injury Severity Score (ISS) in predicting case fatality of 234 patients with major trauma treated during the years 1985 through 1987. Logistic regression was used for determining the cut-points: Death was considered as predicted by ISS if it was greater than 53 or by TI if it was greater then 3.74. Specificity was 95% for ISS and 94% for TI. Sensitivity was 49% for ISS and 73% for TI.

Adult↗
PubMed1998

Flow cytometric detection of EBV (EBER snRNA) using peptide nucleic acid probes.

The application of peptide nucleic acid (PNA) probes for detection of Epstein-Barr Virus (EBV) snRNA in fixed cells is described. Fluorescein labelled PNA probes were used to detect EBER1 and EBER2 snRNA in Raji, Daudi and HS-Sultan cells. The fixation and permeabilization of cells were optimized. The optimal fixation was found to be 5% acetic acid plus 4% paraformaldehyde in PBS and the optimal permeabilization 0.5% Tween 20 in PBS whereas no proteolytic digestion was needed. The hybridization time needed with the PNA probes was only 1 h. When running mixed samples of Ramos (EBV neg.) Raji, Daudi and HS-Sultan (EBV pos.) cells in flow cytometry a strong fluorescence signal was seen in Raji, Daudi and HS-Sultan cells whereas no fluorescence signal was seen in the Ramos cells. In total 0.5% EBER positive Raji cells could easily be identified in a mixture of Raji and Ramos cells. The results were verified by fluorescence microscopy. It is concluded that PNA probes can be used for in situ hybridization in solution and the analysis can be done using flow cytometry or fluorescence microscopy. PNA probes therefore may facilitate and enhance the potential use of the in situ hybridization/flow cytometry combination.

Animals↗
PubMed1993

The 3;21 translocation in myelodysplasia results in a fusion transcript between the AML1 gene and the gene for EAP, a highly conserved protein associated with the Epstein-Barr virus small RNA EBER 1.

In the 8;21 translocation, the AML1 gene, located at chromosome band 21q22, is translocated to chromosome 8 (q22), where it is fused to the ETO gene and transcribed as a chimeric gene. AML1 is the human homolog of the recently cloned mouse gene pebp2 alpha B, homologous to the DNA binding alpha subunit of the polyoma enhancer factor pebp2. AML1 is also involved in a translocation with chromosome 3 that is seen in patients with therapy-related acute myeloid leukemia and myelodysplastic syndrome and in chronic myelogenous leukemia in blast crisis. We have isolated a fusion cDNA clone from a t(3;21) library derived from a patient with therapy-related myelodysplastic syndrome; this clone contains sequences from AML1 and from EAP, which we have now localized to band 3q26. EAP has previously been characterized as a highly expressed small nuclear protein of 128 residues (EBER 1) associated with Epstein-Barr virus small RNA. The fusion clone contains the DNA binding 5' part of AML1 that is fused to ETO in the t(8;21) and, in addition, at least one other exon. The translocation replaces the last nine codons of AML1 with the last 96 codons of EAP. The fusion does not maintain the correct reading frame of EAP and may not lead to a functional chimeric protein.

Amino Acid Sequence↗
PubMed2002

Lack of expression of the Epstein-Barr Virus (EBV) gene products, EBERs, EBNA1, LMP1, and LMP2A, in breast cancer cells.

Epstein-Barr virus (EBV), a gamma herpesvirus, has been associated with a variety of human malignancies such as Burkitt's lymphoma, Hodgkin's lymphoma, NPC, and gastric cancer. A controversy regarding the association of EBV with breast cancers has recently been reported in the literature. These reports have mainly used the DNA detection techniques of polymerase chain reaction and Southern blot hybridization, with the inherent lacunae associated with these techniques for signal localization. Our group has studied EBV association with breast cancer by using in situ hybridization for detecting nonpolyadenylated EBV RNA (EBERs), along with using protein localization technique of immunohistochemistry, studying the EBV nuclear antigen 1 (EBNA1) and the latent membrane proteins (LMP1 and LMP2A). This is the first article analyzing the expression of LMP2A in breast cancer cells. In all of our 43 female breast cancer cases under study, we failed to detect expression of any of the EBV viral gene products tested.

Breast Neoplasms↗
PubMed2005

EBER expression of pure urinary bladder lymphoepithelioma-like carcinoma in two unique Asian patients.

We describe the 2 unique Asian cases of pure urinary bladder lymphoepithelioma-like carcinoma (LELCA). One patient had metastatic pelvic lymphoadenopathy and the other with superficial tumor. The EBER in situ hybridization both showed negative results despite racial or geographical factors might influence the peculiar susceptibility of individuals of Asian ancestry to Epstein-Barr virus-associated lymphoepithelioma-like carcinoma. The 2 patients were treated pertinently with satisfactory outcomes and intravesical BCG instillation is performed to prevent superficial LELCA recurrence rationally.

Aged↗
PubMed1990

An aneurysm in the Papyrus of Ebers (108, 3-9).

One of the most ancient descriptions of arterial aneurysm occurs in a paragraph of the Ebers Papyrus: Egyptians considered it a consequence of a vascular lesion and treated it surgically, through an instrument, which had to be previously cauterized on fire.

Aneurysm↗
PubMed2005

[An investigation of metazoan parasites of common Carp (Cyprinus carpio L.) in Lake Eber, Afyon, Turkey.].

In this study, metazoan parasites of the following fish species were investigated in Lake Eber (Afyon) from July 2002-2003. At the end of this research, 2 species of Monogenea (Gyrodactylus elegans, Dactylogyrus extensus) were found on the gills and fins of the fishes; 1 species of Digenea (Posthodiplostomum cuticola), on skin and fins; 1 species of Cestoda (Bothriocephalus acheilognathi) in the intestines; and 1 species of Arthropoda (Argulus foliaceus), the skin, gills and fins of fish. The distribution of these parasites in various fish species are given below: The distribution of these parasites are as follows: 3456 Gyrodactylus elegans in 51 specimens of common carp (67.1% prevalence, 67.75+/-162.52 parasite/fish); 2980 Dactylogyrus extensus (73.6%, 53.21+/-52.95) in 56 specimens of common carp; 55 Posthodiplostomum cuticola (18.4%, 3.93+/-5.42) in 14 specimens of common carp; 1240 Bothriocephalus acheilognathi (43.4%, 37.55+/-53.98) in 33 specimens of common carp and 6 Argulus foliaceus (6.5%, 1.20+/-0.44); in 5 specimens of common carp.

English Abstract↗
PubMed1996

[Effect of microwave pretreatment on the detection of Epstein-Barr virus EBER RNA's using in situ hybridization].

We have tested the influence of microwave pretreatment of tissue sections on in situ hybridization applied to the detection of Epstein-Barr virus-encoded small RNAs (EBER). To this end, different protocols have been tested on slides of 4 lymph node lymphomas: microwave pretreatment, proteinase K pretreatment, microwave followed by proteinase K and no pretreatment. To compare the sensitivity of each technique, several dilutions of oligoprobes were used. Hybrids have been detected by immunohistochemistry. The analysis of the nuclear stainings obtained shows an enhancement of the sensitivity in protocols using microwaves.

Endopeptidase K↗
PubMed1997

[Varicose veins and methods used to cut them: from the Ebers papyrus to Trendelenburg].

Varicose veins have been described since antiquity. The Ebers papyrus, dating from about 1550 BC reported this disease. Ancient Roman surgeons already performed surgical resection. French surgeon of the Middle Age, Guy de Chauliac, described, in Chirurgica Magna, bandaging for ulcers and cautery or surgical resection for varicose veins. The most remarkable texts were those of Ambroise Paré in the 16th Century, using all these techniques for surgical teaching while abandoning many medical treatments based on concepts of disease as imbalances of "humors". The end of 19th century was the start of new surgical progress with Trendelenburg, Delbert and Mocquot the development of sclerotherapy.

Egypt↗
PubMed2003

[A new measurement of pharmaceutical prescriptions of the Ebers papyrus].

Starting point of this article is a problem obvious to anybody who reads the medical papyri of ancient Egypt from the New Kingdom and the Late Period: the prescriptions contain two dyadic systems of notation for measures of capacity without specifying the unit of measurement, firstly the Horus eye fractions 1/2, 1/4, 1/8, 1/16, 1/32 and 1/64 which are unanimously assumed to refer to the single Heqat and secondly the, normal' dyadic fractions from 1/2 to 1/128, which have been interpreted in widely different ways, as fractions of the Henu-, the Ro-, the single Heqat-, the 5-Ro- or the Dja-measure, respectively. Interpretations suggested in translations and commentaries have remained inconclusive and unsatisfactory.

Drug Prescriptions↗
PubMed2003

Eber- and LMP-1-expressing pulmonary lymphoepithelioma-like carcinoma in a Caucasian patient.

Primary lymphoepithelioma-like carcinoma (LELC) of the lung is a rare tumor that preferentially affects Asian patients, with only 15 cases described in Caucasians to date. Epstein-Barr virus (EBV) infection is present in most lung LELCs in Asians, but it has never been shown in Caucasians. EBV small nuclear RNA and latent membrane protein-1 were identified in the neoplastic cells of primary LELC of the lung diagnosed by endobronchial biopsy in a 25-year-old Italian male. Despite advanced locoregional disease, with neoplastic infiltration of the main right bronchus, the carina, and the pulmonary artery, which prevented surgical resection, partial response and 1-year symptom-free follow-up were achieved after combined chemotherapy and radiotherapy.

Adult↗