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PubMed1997

[Diagnosis of dermatomycoses with polymerase chain reaction].

A polymerase chain reaction (PCR) assay for the detection of dermatophytes was established. The primers "TR1" and "TR2" amplify a 581 bp fragment within the gene coding for the small ribosomal subunit (185 rRNA) of fungi. PCR allowed the detection of isolates of 7 common dermatophytes and in addition several yeasts and moulds. Hybridisation with specific oligonucleotides results in the identification of dermatophytes and Candida albicans. Restriction analysis of the PCR product allowed us to distinguish between dermatophytes and yeasts or moulds. The specificity of the PCR with respect to fungi was assessed by testing human DNA collected from 42 dermis and epidermis specimens as well as DNA from selected plants and animals. To evaluate the clinical relevance of the PCR assay, 69 routinely collected skin and nail specimens were examined by PCR and culture. PCR detected dermatophytes in 35 and culture in 28 of 38 specimens that were classified as positive. Sensitivity of PCR (92%) was higher than that of culture (73%). These results show that PCR has advantages over culture for the detection of dermatophytes.

Arthrodermataceae↗
PubMed2004

Dermatomycoses caused by Nattrassia mangiferae in São Paulo, Brazil.

Nattrassia mangiferae formerly known as Hendersonula toruloidea, is a phaeoid coelomycete described by Nattrass in 1933. We report five cases of N. mangiferae infections in São Paulo, Brazil. This fungus was isolated from interdigital lesions on the feet in one patient, toenails in three cases and fingernails in the other one. The infections were initially considered to be caused by a dermatophyte. Although there are only a few cases described in the medical literature, the five cases reported suggest that N. mangiferae should be taken into consideration in the differential diagnosis of tinea pedis infections.

Adult↗
PubMed1999

Epidemiology of dermatomycoses of humans in central Poland. Part IV. Onychomycosis due to dermatophytes.

The total number of dermatophytoses (7393) included 1567 (21.2%) cases of onychomycosis. Etiological factors in descending order were: Trichophyton rubrum (58.8%), Trichophyton mentagrophytes var. granulosum (26.2%), T. mentagrophytes var. quinckeanum, T. mentagrophytes var. interdigitale (5.1%), Trichophyton tonsurans (4.1%), Trichophyton violaceum (1.5%), Trichophyton spec. (1.1%). An increase in the incidence was noted between 1994 and 1996. At present, onychomycosis is third by incidence among all clinical forms of dermatophyte infections of skin and skin appendages in the Lódź region.

Adolescent↗
PubMed1999

Epidemiology of dermatomycoses of humans in central Poland. Part V. Tinea corporis.

The total number of dermatophytoses (7393) included 2204 (29.8%) cases of tinea glabrosa. Etiological factors in descending order were: Microsporum canis (23.5%), Trichophyton mentagrophytes var. granulosum (21.6%), Trichophyton rubrum (17.8%), Trichophyton tonsurans (10.4%), Epidermophyton floccosum (7.7%), T. mentagrophytes var. quinckeanum (6.0%), Microsporum gypseum (5.3%), Trichophyton violaceum (3.7%), T. mentagrophytes var. interdigitale (2.3%), Microsporum equinum (0.7%), Trichophyton verrucosum (0.4%), Trichophyton spec. (0.4%), Microsporum cookei (0.14%). At present tinea glabrosa is dominant among all clinical forms of dermatophyte infections of skin and skin appendages in the Lódź region.

Adolescent↗
PubMed2002

Prevalence of dermatomycoses in Mal de Meleda patients: a field study.

Mal de Meleda is a rare autosomal recessive form of palmoplantar keratoderma characterized by hyperkeratosis of the palms and soles. The presence of yeast and dermatophytes was investigated in 29 mal de Meleda patients from Koprucay canyon, Turkey, a newer geographical focus, and was found in 62.0% and 20.7% of cases, respectively. Antifungal resistance of isolates was not detected.

Adult↗
PubMedDate not supplied

Treatment of dermatomycoses with ketoconazole.

Forty patients (22 males and 18 nonpregnant females) with tegumentary mycoses were treated with ketoconazole (R41,400). The group included 39 patients with dermatophytoses and one with tinea versicolor. Ketoconazole was administered in one dose per day taken with water 2 hr before or after breakfast for one month; patients weighing < 30 kg received 100 mg of ketoconazole per day, whereas those weighing > 30 kg received 200 mg per day. Twenty-one patients had complete clinical and mycologic cure, two responded clinically but the last culture was positive, eight had partial improvement, and three had no improvement at all. In six cases the treatment was stopped (in one because of gastric intolerance). The main adverse effect of ketoconazole was nausea; only one patient had vomiting. The results indicate that ketoconazole is a safe and effective drug for treatment of dermatomycosis.

Adolescent↗