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Fine vasculature of hepatoma in Chinese hamsters (Cricetulus griceus); scanning electron microscopy of resin casts of blood vessels.

Scanning electron microscopy of resin casts was carried out on the vascular system of hepatoma in Chinese hamster. Casts of capillaries in the hepatoma were thin and fragmentary and formed a fine randomly arranged network in contrast to those in the liver with radial arrangement and central veins in centrilobular regions. Capillaries in the cancer merged into superficial venules corresponding to central veins while those in the border region merged into sinusoids of adjacent liver tissue. The superficial venules connected to tributaries of hepatic veins to drain into the posterior vena cava. Neovascularization did not occur by sprouting and anastomosing to form hair-pin loops of capillaries but by division of pre-existing vessels into smaller vessels, thus increasing the number of capillaries. The hepatic artery feeding the cancer was seemingly intact. There was no indication of arterio-portal shunts in the hepatoma.

Animals↗

Susceptibility of Chinese hamsters (Cricetulus griseus) to the infection of Babesia microti.

Chinese hamsters were examined for the susceptibility to the infection with Babesia microti based on the hematological parameters during the course of infection. A marked decrease in the RBC count, Ht value, Hb concentration, and an increase in WBC count due to the development of neutrophils or monocytes were recognized with the progress of parasitemia. Remarkable clinical findings were anemia and persistent infection with a low level of parasite burden in the chronic and convalescent stages. From these findings, it was concluded that Chinese hamsters were susceptible to infection with B. microti and would be useful for infection examination with the parasite.

Animals↗

Molecular cloning and sequences of interleukin-10 in the Djungarian (Phodopus sungorus), Chinese (Cricetulus griseus), and Syrian (Mesocricetus auratus) hamster.

Interleukin 10 (IL-10) genes of Djungarian, Chinese, and Syrian hamsters were cloned. The clones of IL-10 consisted of 537 bp nucleotides and 178 amino acids in full length, and the nucleotide and amino acid sequences exhibited a high degree of homology with those of the mouse and human. Since the number and position of signal sequences, N-glycosylations and cysteine sites in the IL-10 amino acid sequences of the hamsters were the same as those of the mouse, we suggest that the IL-10 molecular structures of the hamster are closer to that of the mouse than human.

Amino Acid Sequence↗

Fine structure of the parotid gland of Chinese hamster (Cricetulus griseus).

The parotid gland of Chinese hamster was examined by light and electron microscopy. The parotid acinar cells contained acidophile granules and reacted with the periodic acid-Schiff (PAS) reagent but failed to stain with alcian blue (AB). The acini were composed of light, dark and specific light cells containing secretory granules of various sizes and densities. Intercalated duct cells were composed exclusively of light cells and contained a few vesicles, fine granules and polygonal granules. Striated duct cells were PAS-positive and AB-negative, being composed of light and dark cells. These cells contained vesicles and fine granules, and the light cells in the male contained electron dense polygonal granules. No myoepithelial cells were detected around the acini, intercalated ducts or striated ducts, while nerve terminals were demonstrable among adjacent acinar cells or between the acinar cells and the underlying basement lamina.

Animals↗

Fine structure of the mandibular gland of Chinese hamster (Cricetulus griseus).

The mandibular glands of the Chinese hamster were examined by light and electron microscopy. The mandibular acinar cells were noted to be categorized in a seromucous type, because the acinar cells stained slightly basophile and moderately positive for periodic acid-Schiff (PAS) and alcian blue (AB) reactions and the intercellular canaliculi were detected in the cells. The acini were composed exclusively of light cells containing various secretory granules. The acinar cells exhibited a sexual dimorphism; in the female AB staining was more intense, and also the granules having the matrix of a fine hair-like material were more numerous than in the male. Intercalated duct cells were composed exclusively of light cells and contained round and polygonal granules. The striated ducts consisted of two distinct portions, the secretory and typical striated portions without secretory granules. The epithelial cells of secretory portion were columnar in shape and contained acidophile granules which gave a strongly positive PAS reaction, and comprised light and dark cells containing secretory granules varying in size and density. The epithelium of typical striated portion was composed of both light and dark cells containing fine vacuoles and vesicles, but no secretory granules. No sexual dimorphism was detected in the striated duct.

Animals↗

Chondrosarcoma of the mandible and falx cerebri in a Chinese hamster (Cricetulus griceus): report of a case.

A Chinese hamster (male, 1-month-old) was found to have a large nodular mass at the left mandible. The tumor appeared to replace the entire left mandible, extending from the third molar to the incisor. The tumor mass occupied most of the oral cavity to compress the tongue ventrally. Histologically, the neoplasm was composed of islands of chondrocytes with bizarre and enlarged nuclei which were surrounded by poorly differentiated mesenchymal cells. The tumor involved the falx cerebri to compress the frontal crest of the cerebrum. On the basis of histological features, this tumor was diagnosed as chondrosarcoma.

Animals↗

Mitotic variations of the stem cell and its renewal in the Chinese hamster Cricetulus griseus during spermatogenesis: a morphological study.

The stem cell As in the testicular tubule of the Chinese hamster has been identified morphologically in its proliferation and in its transitional variations up to the formation of the primary spermatocyte by means of hypotonics alone. An extensive study of nuclei in their telophase has allowed us to trace the transition of mitosis into meiosis in several stéps. The presence of nuclei in an apparent inactive or "resting" stage strongly supported the renewal of the As stem cell and gave a clue to the presence of an inherent factor in the tubule that regulates the timing of the cycle of the seminiferous epithelium.

Animals↗