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At least 73 records · Page 4Linked to original sources

Solid-phase peptide synthesis: a silver anniversary report.

It has been a quarter of a century since Merrifield's initial report on solid-phase peptide synthesis. The field has matured significantly in recent years with a better understanding of the underlying chemistry. This is reflected by new, milder orthogonal protection schemes and more efficient coupling methods, some of which have been incorporated into automated systems. Advances in purification, especially high performance liquid chromatography, have had a major impact. The efficacy of these improvements has been demonstrated by an impressive litany of applications to biological problems.

Methods↗

[Recent concept of protection of workers and general population against electromagnetic fields in the European countries].

In 1994, guidelines for protection against EM fields were elaborated by the International Commission for Non-Ionizing Radiation Protection (ICNIRP). In 1998, they were slightly modified and became the base for the 1999 recommendation of the UE Commission. The ICNIRP recommendations are based on few selected criteria, and thus provide good protection in the case of short-term exposures to EM fields, but possible effects of EM fields at intensities lower than those admissible by the ICNIPR standards raise still growing doubts. Over a couple of years, well educated and organized groups of citizens and non-governmental organizations call attention to risks of long-term exposure of humans to weak EM fields. In their reviews, the members of these groups/organizations draw attention to the need for a precautionary approach on the one hand, and postulate better protection of the general population and workers from weak EM fields on the other. The evaluation of the present situation indicates that the range of the influence of independent groups/organizations supporting the modification of ICNIPR guidelines to include the effects of low-level EM fields is steadily growing.

Electromagnetic Fields↗

Chemical and enzymatic synthesis of glycopolymers.

The development of efficient, fast, flexible and general synthetic routes to glycopolymers is an ongoing challenge and much progress has been made in recent years. Chemical coupling methods have become increasingly sophisticated to fine-tune reactivity of reagents by fortuitous choices of anomeric activating group and protecting groups. As a result, oligosaccharide synthesis has become more predictable and reliable even to the extent that first examples of saccharide library syntheses in solution and on the solid phase have been published. In biology, the repertoire of biocatalysts that can be used for glycoside synthesis is ever-increasing, and enzyme-catalysed glycosylation steps have been successfully incorporated into synthetic strategies.

Animals↗

[Blood lead level of children in the urban areas in China].

OBJECTIVE: The purpose of this study was to investigate the blood lead level of 3 - 5 year old children living in the cities in China and to provide scientific data for making policy on environmental lead pollution for children health protection. METHODS: Six thousand five hundred and two vein blood samples from 3 - 5 year old children in nineteen cities of nine provinces were sampled. Inductively coupled plasma-mass spectrometry (ICP-MS) were employed to determine lead level in whole blood after microwave digestion for sample preparation and questionnaire survey was also performed. Data were analyzed with multiple regression on factors which affecting blood lead levels. RESULTS: Results showed that mean blood lead level was 88.3 micro g/L for 3 - 5 year old children living in the cities in China and mean blood lead level of boys (91.1 micro g/L) was higher than that of girls (87.3 micro g/L). Twenty-nine point nine one per cent of the children's blood lead level exceeded 100 micro g/L. The research finding showed: (1) higher blood lead levels had negative effects on children's physical growth, language ability etc. (2) behavior of parents had certain effects on children's blood lead levels. (3) blood lead levels of children were affected by unhealthy habits. CONCLUSIONS: Problem of childhood lead poisoning in China has become more serious. During the past ten years, blood lead levels of children has been increased in China while decreasing in developed countries. Blood lead levels of children in China are higher than that of developed countries, which called for special concern by government and society.

Child, Preschool↗

[Intrauterine contraceptive devices (IUD'S) (author's transl)].

The IUD is an excellent and very effective contraceptive method. As contraception and coitus are separated, this technique is acceptable to many couples. It provides prolonged protection and cannot be "forgotten" like the Pill, the diaphragm, or the condom. Its pregnancy rate is 2 per 100 women per year. The greatest clinical experience exists with the Lippes loop. The loop should be the method of choice for parous women, the Copper-T for the nullipara and nulligravida. Regularly menstruating women should be advised to use additional contraceptive protection (condom, diaphragm, coitus interruptus, foam) during the week of highest fertility. This will make the IUD as effective as the Pill. Physicians prescribing this method should be available 24 hours per day for their patients, as emergency situations such as P.I.D., ectopic pregnancy, septic abortion--rare as they are--would need prompt attention.

Coitus↗

New approaches to the chemical synthesis of bioactive oligosaccharides.

The past year has seen some major advances in the area of carbohydrate synthesis using chemical methods. Progress in all areas of synthetic methodology, including new protecting groups and coupling methods, has been reported. A number of complex carbohydrate structures have been prepared using known, as well as new, methods. The goal to allow nonspecialists access to defined carbohydrate structures for biochemical, biophysical and biological studies has drawn closer by the introduction of two approaches towards synthesis automation. A one-pot glycosylation strategy utilized computer-assisted synthesis planning and the first solid-phase automated synthesizer was introduced very recently.

Carbohydrate Sequence↗

In search of new methods in peptide synthesis. A review of the last three decades.

An attempt was made to discern ideas and trends in the development of peptide synthesis and to recognize general principles of the discipline. Introduction of efficient methods of activation and coupling during the early years of the reviewed period was followed by only moderate further improvements. Major advances were achieved by the discovery of novel methods of protection and by techniques of facilitation. Improvements in the methods of deblocking hold considerable promise and might bring significantly closer the goal of peptide synthesis: the direct preparation of homogeneous products.

Amino Acid Sequence↗

Measles and mumps vaccination as a model to investigate the developing immune system: passive and active immunity during the first year of life.

Evaluations of neutralizing antibody responses in 6-, 9- and 12-month-old infants given measles or mumps vaccine indicated that 6-month-old infants had diminished humoral immune responses associated with passive antibody effects, but also had an intrinsic deficiency in antiviral antibody production, which was independent of passive antibody effects. In contrast, lower neutralizing antibody titers in 9-month-olds were related only to passive antibody effects. Measles and mumps-specific T-cell proliferation and interferon-gamma (IFNgamma) production were induced by vaccination at 6, 9 or 12 months, regardless of passive neutralizing antibodies or age. These observations suggest a need to refine concepts about passive antibody interference and primary vaccine failure, taking into account the sensitization of antiviral T-cells, which occurs in the presence of passive antibodies and is observed in infants who do not develop active humoral immunity. A second dose of measles vaccine given at 12-15 months enhanced antiviral T-cell responses to measles in infants who were vaccinated at 6 or 9 months, and produced higher seroconversion rates. Since T-cell immunity is elicited under the cover of passive antibodies, the youngest infants benefit from the synergistic protection mediated by maternal antibodies and their own capacity to develop sensitized antiviral T-cells, which prime for subsequent exposures to the viral antigens. Conceptually, maternal immunization approaches with vaccines that can be given to women of child-bearing age before pregnancy, or that are safe for administration during pregnancy, should enhance passive antibody protection. Rather than being detrimental to infant adaptive immune responses, maternal vaccination can be coupled effectively with vaccine regimens that elicit priming of antiviral immune responses in infants during the first year of life.

Aging↗

Management of the risk of HIV infection in male homosexual couples.

OBJECTIVES: To study the ways of managing HIV risk within male homosexual steady relationships (gay couples), including factors associated with consistent condom use during anal sex with the steady partner. METHOD: An anonymous and standardized questionnaire completed by a convenience sample of homosexuals in Switzerland in 1997 (n = 1097). Information on the couple was provided by the 74% (n = 786) of male respondents who reported having a steady partner in the past 12 months. Data were analysed by contingency tables and logistic regression. RESULTS: Different ways of managing HIV risk were reported: negotiated safety (both HIV negative, condoms abandoned) was chosen by one quarter of the couples, but the most frequent solution was reliance on condoms for anal sex, chosen by more than four in 10. Altogether 84% of couples exhibited safe management of HIV risk within their partnership. The 16% of couples showing inadequate management of HIV risk within the couple mostly relied on questionable assumptions about past or present risks. A total of 74% of couples had spoken about managing HIV risk with possible casual partners. Reported behaviour with the steady partner and with casual partners was highly consistent with claimed strategies chosen to manage HIV risk. Consistent condom use with the steady partner was mostly associated with variables characterizing the relationship: initial 2 years of the relationship, discordant or unknown serological HIV status, non-exclusivity. CONCLUSION: Gay couples manage HIV risk in a variety of ways. Most strategies provide adequate protection with casual partners, but leave gaps in protection between the steady partners themselves.

Adult↗

Improved communications and hearing protection in helmet systems: the communications earplug.

Despite significant advances in hearing protection and compliance with protective standards, military personnel are still subject to noise-induced hearing loss in many combat and combat support operations. Although the Army has experienced a decrease of some 15% in primary hearing loss disability cases since 1986, a fiscal year 2000 report documents a 27.5% increase in audiograms, which demonstrated significant threshold shifts in assessed personnel (N = 841/1,077). Compensation for noise-induced hearing loss disability for the Army alone exceeded $180 million in 1998. Thus, communications and hearing protection remain critical issues for personnel involved in Army operations. Aircraft, ground vehicles, and weapons produce noise levels in excess of the limits defined in current hearing conservation standards. Performance of most helmets, improved over the years, remains marginal with regard to speech intelligibility. Furthermore, these helmets do not provide adequate hearing protection. The communications earplug, which consists of a high-quality earphone coupled with an earplug protector, provides the needed extra protection. It weighs less than 15 g and is comfortable when worn over extended periods. It is considered highly acceptable by seasoned Army aviators and crewmembers.

Communication↗

Behavior modification obtained by sun protection education coupled with removal of a skin cancer.

The 1042 people in this study who had a nonmelanoma skin cancer surgically removed received repetitive and interactive oral and written education in forms of sun protection. One year after these educational efforts, the subjects responded to a questionnaire that measured their compliance. Sixty-two percent complied with starting to use sunscreens and 56% changed their habits of outdoor activities. The portion of behavioral change attributable to surgery alone vs surgery plus education was unable to be determined. Despite intensive educational efforts, the noncompliance was 38%. This study identifies a health risk-taking pattern of behavior among those who were noncompliant. Those over age 65 years tended to be less compliant. When the data were analyzed by the transtheoretical model of behavior change, those already acting on some form of sunscreen use prior to this study achieved 100% compliance with increasing to a sunscreen with a sun protection factor of 15 or greater. It is hoped that with more educational efforts those in precontemplative and contemplative phases of behavior can be recruited to take action, and those who acted by acquiring one sun protective behavior will add other behaviors.

Adult↗

Ten years of experience in periconceptional care.

OBJECTIVES: To describe the methods of the periconceptional care, consisting of counselling, examinations and medical intervention in Hungary. The term periconceptional is used instead of preconceptional because early postconceptional period is also involved to this service. METHODS: A model was developed based on three steps: check-up of reproductive health (i.e. preconceptional screening), the 3-month preparation for conception, and better protection in early pregnancy for the most sensitive early development of embryo for voluntary and eligible couples. Data of 8837 female and 7600 male participants from the coordinating centre of the Hungarian periconceptional care between 1 February 1984 and 31 January 1994 are summarized. Mean maternal age was 25.8+/-3.4 years, 84 and 60% of female participants were primiparae, and had high education (> or = 13 classes), respectively. RESULTS: It was possible to establish periconceptional care performed and/or supervised by qualified nurses. Participants with positive family history, case history and where genitourinary infections were detected, had a more effective flow towards secondary care. Infertile couples were diagnosed and treated sooner. The periconceptional care is effective for the introduction of periconceptional folic acid/multivitamin supplementation and for the reduction of smoking and alcohol consumption in females in the preconceptional period. The rate of major congenital abnormalities (20.6/1000) was significantly lower than expected (35/1000). CONCLUSIONS: Periconceptional care is feasible and has many benefits. Thus, proper preparation for conception is the earliest and probably the most important method of health promotion in general, particularly for the prevention of congenital abnormalities.

Adult↗

Malaria morbidity among school children living in two areas of contrasting transmission in western Kenya.

Research in malaria-endemic areas is usually focused on malaria during early childhood. Less is known about malaria among older school age children. The incidence of clinical attacks of malaria was monitored, using active case detection in primary schools, in two areas of western Kenya that differ in the intensity of transmission. Clinical malaria was more common in schools in the Nandi highlands, with a six-fold higher incidence of malaria attacks during the malaria epidemic in 2002, compared with school children living in a holoendemic area with intense perennial transmission during the same period. The high incidence coupled with the high parasite densities among cases is compatible with a low level of protective immunity in the highlands. The malaria incidence among school children exposed to intense year-round transmission (26 per 100 school children per year) was consistent with reports from other holoendemic areas. Taken together with other published studies, the data suggest that malaria morbidity among school age children increases as transmission intensity decreases. The implications for malaria control are discussed.

Adolescent↗

Couple resilience to economic pressure.

Over 400 married couples participated in a 3-year prospective study of economic pressure and marital relations. The research (a) empirically evaluated the family stress model of economic stress influences on marital distress and (b) extended the model to include specific interactional characteristics of spouses hypothesized to protect against economic pressure. Findings provided support for the basic mediational model, which proposes that economic pressure increases risk for emotional distress, which, in turn, increases risk for marital conflict and subsequent marital distress. Regarding resilience to economic stress, high marital support reduced the association between economic pressure and emotional distress. In addition, effective couple problem solving reduced the adverse influence of marital conflict on marital distress. Overall, the findings provided substantial support for the extended family stress model.

Adaptation, Psychological↗

Psychopathology and attrition in the Baltimore ECA 15-year follow-up 1981-1996.

Predictors of non-response were investigated in a 15-year follow-up (1981-1996) of 3,481 individuals in a probability sample from the household population of East Baltimore. Demographics (age, sex, race, education, marital status, and unemployment), household factors (living arrangements, household income, household size, and number of children), cultural variables (ancestral ethnicity and foreign language), social variables (social support and networks, committing felony, carrying a weapon, using an alias, and wandering), health factors (physical illness, health insurance, medical assistance, Medicare, receiving disability benefits, social security, and welfare), interviewer's observation, and psychopathologic variables (mental disorders, suicide behavior, comorbidity, and drug use) were collected at baseline in 1981 and in 1982, then linked to follow-up data between 1993 and 1996. A tracing process involving mail, phone, criss-cross directories, motor vehicle administration records, a commercial credit bureau, the state criminal justice system, hospital records, the US National Death Index, and field tracing were used to locate the original sample. A total of 3,066 respondents of the original sample (88.1%) were traced. Non-response was categorized into Sample Mortality (that part of the original sample that died during follow-up), Sample Loss (that part of the original sample that survived but could not be found) and Refusal (that part of the original sample that survived and was found but refused to participate). Stratified analysis and adjusted multiple logistic regression modeling found sample mortality and sample loss were strongly influenced by individual and household variables and by psychopathology. Sample mortality was influenced by specific mental disorders or conditions as mania, drug abuse/dependency, antisocial personality, cognitive impairment, alcohol abuse/dependency, phobia, drug use (except PCP), and comorbidity. Household factors protective against mortality include higher household income, not living as extended members in a married couple family, and living with children in the household. Persons who were unemployed, widowed or single, without high school education, male, and 65 years of age or older were more likely to die. Sample loss was influenced by cognitive impairment, antisocial personality, and cocaine use. Household factors linked to sample loss include living in female-headed families, or non-family households, and living alone. Young nonwhite, divorced/separated, without high school education, and unemployed were also harder to find. Refusal was associated with being white, with incomplete elementary education, living as a spouse in traditional married couple families, or as a child in female-headed families. Psychopathology did not influence refusal.

Adolescent↗

Pregnancy-associated malaria: parasite binding, natural immunity and vaccine development.

Humans living in areas of high malaria transmission gradually acquire, during the early years of life, protective clinical immunity to Plasmodium falciparum, limiting serious complications of malaria to young children. However, pregnant women become more susceptible to severe P. falciparum infections during their first pregnancy. Pregnancy associated malaria is coupled with massive accumulation of parasitised erythrocytes and monocytes in the placental intervillous blood spaces, contributing to disease and death in pregnant women and developing infants. Indirect evidence suggests that prevention may be possible by vaccinating women of childbearing age before their first pregnancy. This review aims to introduce the reader to the implications of malaria infection during pregnancy and to analyse recent findings towards the identification and characterisation of parasite encoded erythrocyte surface proteins expressed in malaria-infected pregnant women that are likely targets of protective immunity and have potential for vaccine development.

Animals↗

Effect of marital quality on eight-year survival of patients with heart failure.

Recent evidence suggests that psychosocial factors such as self-efficacy, psychological distress, perceived social support, and marital quality have prognostic significance for morbidity and mortality after heart failure. Previously, we reported that interview and observational measures of marital quality obtained from 189 patients with heart failure (139 men and 50 women) and their spouses predicted all-cause patient mortality during the next 4 years, independent of the baseline illness severity (New York Heart Association class). We present additional follow-up results for this sample, with Cox regression analyses showing that a couple-level composite measure of marital quality continued to predict survival during an 8-year period (p <0.001), especially when the patient was a woman, and did so substantially better than individual (patient-level) risk and protective factors, such as psychological distress, hostility, neuroticism, self-efficacy, optimism, and breadth of perceived emotional support. In conclusion, relationship factors may be especially relevant in managing a difficult chronic condition such as heart failure, which makes stringent and complex demands on patients and their families.

Adult↗

Maternal-fetal interaction in the ABO system: a comparative analysis of healthy mothers and couples with recurrent spontaneous abortion suggests a protective effect of B incompatibility.

We investigated the possible differential effects of A and B blood group materno-fetal incompatibility on human fertility through a comparative analysis of couples with recurrent spontaneous abortion (RSA) and healthy mothers. ABO phenotype was determined in 5180 healthy mothers and their newborn babies from the population of Sassari (Sardinia) and in 1359 healthy puerperae (women who have just given birth) from the population of Rome. Mother-newborn joint ABO distribution in healthy mothers was compared with wife-husband joint ABO distribution in RSA couples. Distortions from expected distribution were evaluated by symmetry analysis. In both RSA couples and healthy mothers significant deviation from expected symmetry patterns were observed. Deviations in RSA are in the opposite direction to those observed in healthy puerperae. The most important difference observed concerned the symmetric joint phenotypes mother (women) A/infant (husband) B (B incompatible) and mother (women) B/infant (husband) A (A incompatible). A low number of B incompatible in RSA couples and a high number of B incompatible in healthy mothers was observed. The phenomenon is much more evident in women aged 24-28 years, a period of maximum fecundity. It is possible that the presence of anti-B immunoglobulin in the mother might have a protective effect against fetal loss in some cases of mother-infant ABO incompatibility.

ABO Blood-Group System↗