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Attachment and growth of Salmonella Chester on apple fruits and in vivo response of attached bacteria to sanitizer treatments.

Attachment and growth of Salmonella Chester on fresh-cut apple disks and in vivo response of attached bacteria to sanitizer treatments were investigated. Apple disks (14 mm in diameter and 3 to 4 mm in thickness) were immersed in a bacterial suspension that contained 8.17 log CFU/ml of Salmonella Chester and air dried at room temperature for 10 min. After two rinses, the population of Salmonella Chester retained on apple disks that contained no skin was 13 to 19% higher than that retained on disks that contained skin, indicating that Salmonella Chester attached more firmly to the surfaces of injured tissue than to the unbroken skin. The number of bacteria attached to the disk was not affected by the immersion time but was directly proportional to the concentration of bacteria in the suspension. The distribution of artificially inoculated Salmonella Chester on the surfaces of three different parts of whole fruit was determined; 94% of attached bacteria was found on the stem and calyx cavity areas and 6% on the skin of the remaining area of the fruit. Despite their acidic pH (4.1), apple disks supported the growth of Salmonella Chester at 20 degrees C but not at 8 degrees C. All four sanitizers tested in the study, including 6% hydrogen peroxide, 2% trisodium phosphate, 0.36% calcium hypochlorite, and 1.76% sodium hypochlorite, were effective in reducing the population of Salmonella Chester on apple disks by 1 to 2 logs. However, 5 to 13% of bacteria survived the sanitizer treatments. Hydrogen peroxide, which reduced the population of Salmonella Chester on skin by 3 to 4 logs and the population of bacteria on stem or calyx by 1 to 2 logs, was the most effective among the four sanitizers tested. Firm attachment of bacteria on calyx, stem, and injured tissue and partial resistance of attached bacteria to sanitizer treatments are two major obstacles to be considered when developing methods for cleaning and decontaminating apple fruits destined for juice production and fresh consumption.

Animals↗

Childhood attachment and abuse: long-term effects on adult attachment, depression, and conflict resolution.

OBJECTIVE: The primary aim was to determine the relative contributions of early attachment and abuse history to adult attachment, depression, and conflict resolution behaviors. Differences between abused and nonabused respondents were also assessed. METHOD: A multi-scale questionnaire was completed by 879 college students. Hierarchical regression analyses were used to answer the primary research question, and analyses also compared the 26.4% of respondents who reported childhood abuse with those who did not. RESULTS: Respondents who indicated they had been abused as children reported less secure childhood and adult relationships than their nonabused counterparts. They were also more depressed and more likely to use destructive behaviors in conflict situations. Although both adult romantic attachment and respondents' depression scores were best accounted for by childhood attachment to mother and father rather than abuse history, the opposite pattern of results emerged for conflict resolution behaviors. In this case, abuse history was the stronger predictor, and parental attachment did not account for any significant additional variance. CONCLUSIONS: Results suggest that the long-term impact of childhood abuse may be mediated by early attachment experiences, whereas the long-term impact of abuse on conflict resolution behaviors may be considerably more direct.

Adaptation, Psychological↗

Within-person variation in security of attachment: a self-determination theory perspective on attachment, need fulfillment, and well-being.

Attachment research has traditionally focused on individual differences in global patterns of attachment to important others. The current research instead focuses primarily on within-person variability in attachments across relational partners. It was predicted that within-person variability would be substantial, even among primary attachment figures of mother, father, romantic partner, and best friend. The prediction was supported in three studies. Furthermore, in line with self-determination theory, multilevel modeling and regression analyses showed that, at the relationship level, individuals' experience of fulfillment of the basic needs for autonomy, competence, and relatedness positively predicted overall attachment security, model of self, and model of other. Relations of both attachment and need satisfaction to well-being were also explored.

Adaptation, Psychological↗

Attachment in individuals with social anxiety disorder: the relationship among adult attachment styles, social anxiety, and depression.

Despite their apparent implications for social functioning, adult attachment styles have never been specifically explored among persons with social anxiety disorder. In the current study, a cluster analysis of the Revised Adult Attachment Scale (N. L. Collins, 1996) revealed that 118 patients with social anxiety were best represented by anxious and secure attachment style clusters. Members of the anxious attachment cluster exhibited more severe social anxiety and avoidance, greater depression, greater impairment, and lower life satisfaction than members of the secure attachment cluster. This pattern was replicated in a separate sample of 56 patients and compared with the pattern found in 36 control participants. Social anxiety mediated the association between attachment insecurity and depression. Findings are discussed in the context of their relevance to the etiology, maintenance, and cognitive-behavioral treatment of social anxiety disorder.

Adult↗

The development and preliminary validation of a new measure of adult attachment: the adult attachment projective.

In this paper, we describe the development of and our preliminary work to empirically validate the Adult Attachment Projective (AAP), a new adult attachment classification system that is based on the analysis of individuals' responses to a set of seven attachment-related drawings. The AAP classification system uses evaluations of three dimensions (Discourse, Content and Defensive Processing) to designate four major adult classification groups: Secure, Dismissing, Preoccupied, and Unresolved. Preliminary validation of the AAP is based on 75 participants drawn from three separate samples. The results indicate strong interjudge reliability and convergent agreement between the AAP and Adult Attachment Interview (AAI) classifications. The AAP, thus, appears to be a promising developmental measure for assessing the representation of attachment in adults. Contributions of the AAP to attachment theory and research are discussed. The similarities and differences between AAP stories and other child and adult representational measures are also discussed.

Adult↗

Early preventive attachment-oriented psychotherapeutic intervention program with parents of a very low birthweight premature infant: results of attachment and neurological development.

The birth of a very small preterm infant (< or = 1500 grams) can be a traumatizing experience for many parents. A developmental risk model is presented that is the background to an early attachment-oriented preventive psychotherapeutic intervention. This comprehensive parent-centered intervention program is composed of supportive group psychotherapy, attachment-oriented focal individual psychotherapy, a home visit and video-based sensitivity training. The intervention aims at improving parental coping, the process of attachment and parent-infant interaction. In a prospective longitudinal design mothers were randomly assigned to a control (N = 44) and an intervention group (N = 43) after preterm delivery. Results show that the percentage of secure (control vs. intervention group: 77.8% vs. 59.4%) and insecure (control vs. intervention group: 8.3% vs. 31.3% avoidant, 13.9% vs. 9.4% ambivalent) attachment quality in high-risk preterm infants is comparable to results from studies with term infants. There was no significant statistical difference in terms of quality of attachment of the preterm infants between the control group and the intervention group. However, only in the control group, impaired neurological development corresponded significantly with an insecure quality of attachment, but not in the intervention group, although there were significantly more neurologically impaired infants in the intervention group. This result is discussed as an effect of the intervention program.

Adaptation, Psychological↗

Maternal representations of attachment during pregnancy predict the organization of infant-mother attachment at one year of age.

While strong retrospective and concurrent associations between maternal and infant patterns of attachment have been noted, this is one of the first reports of a prospective investigation of such associations. The Adult Attachment Interview was administered to 100 mothers expecting their first child, and, at 1-year follow-up, 96 of these were seen with their infants at 12 months in the Strange Situation. Maternal representations of attachment (autonomous vs. dismissing or preoccupied) predicted subsequent infant-mother attachment patterns (secure vs. insecure) 75% of the time. These observed concordances, as well as the discordances, are discussed in terms of the uniquely powerful contribution the Adult Attachment Interview makes to the study of representational and intergenerational influences on the development of the infant-mother attachment.

Adult↗

The association between parental reports of attachment style and family dynamics, and offspring's reports of adult attachment style.

This research assessed the association between parents' reports of attachment styles and their perceptions of family environment, on the one hand, and offspring's reports of adult attachment styles, on the other. The sample included 98 Israeli young adults who completed the adult attachment style scale, and their mothers and fathers who completed this scale, as well as FACES III, and the conflict and expressiveness subscales of the Family Environment Scale. Findings revealed associations between parents' and offspring's reports of attachment styles, which were qualified by gender matching. They also indicated independent and differential contributions of the examined dimensions of perceived family environment to offspring's attachment styles. The discussion attempts to integrate attachment theory with a family system perspective.

Adolescent↗

Glycosphingolipids in detergent-insoluble substrate attachment matrix (DISAM) prepared from substrate attachment material (SAM). Their possible role in regulating cell adhesion.

The glycosphingolipids isolated from the detergent-insoluble material (DIM) of whole cells as well as from a similar detergent-insoluble substrate attachment matrix (DISAM) have been investigated in comparison with the glycosphingolipids of whole cells. The proportion of glycolipids in the total lipid extract was enriched in the DISAM as well as DIM fractions as compared to whole cells. The ratio of ganglioside (GM3) to neutral glycolipids was also higher in the DISAM fractions than in whole cells. The radioactivity incorporated into DISAM glycolipids of BHK cells, metabolically labeled with radioactive glucosamine, was greater in confluent cells than in sparsely growing cells; however, label incorporation into glycolipids of the DISAM fraction of BHKpy cells was 2-3-fold higher than that of confluent BHK cells, although the chemical quantity of GM3 in whole cells was much lower in BHKpy cells than in BHK cells. In order to confirm the enhanced label in DISAM glycolipids of BHKpy cells by other procedures, the labeled cells were detached by EGTA, washed, and reattached on plates. The amount of label in DISAM glycolipids of the reattached matrix of BHKpy cells was much higher than that of BHK cells. Cell spreading and cell attachment on plastic plate were inhibited by inclusion of GM3 in the medium. These data suggest that: (i) glycolipids, particularly GM3, at the cell attachment site have different metabolic activity from those of whole cells; the label in glycolipids goes preferentially into cell attachment sites, and may have some functional role in regulating cell attachment of BHK cells; (ii) metabolic activity and turnover of GM3 in cell attachment sites of confluent cells are higher than actively growing cells, yet those of transformed cells are much higher than any state of non-transformed cells.

Animals↗

Attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment.

Bacterial cell attachment to the surfaces of self-assembled monolayers formed by the adsorption of omega-substituted alkanethiols on transparent gold films has been studied under defined bacterial culture and flow conditions. Phase contrast microscopy was used to quantify the attachment of two organisms, one of medical (Staphylococcus epidermidis) and one of marine (Deleya marina) importance. Self-assembled monolayers terminated with hexa(ethylene glycol), methyl, carboxylic acid and fluorocarbon groups were investigated. Over the range of experimental conditions, self-assembled monolayers formed from HS(CH2)11(OCH2CH2)6OH were found to be uniformly resistant to bacterial attachment, with a 99.7% reduction of attachment for both organisms when compared to the most fouled surface for each organism. On other surfaces, S. epidermidis and D. marina were shown to exhibit very different attachment responses to the wettability of the substratum. While the attachment of S. epidermidis correlated positively with surface hydrophilicity, D. marina showed a preference for hydrophobic surfaces. This study suggests that surfaces incorporating high densities of oligo(ethylene glycol) are good candidates for surfaces that interact minimally with bacteria.

Bacterial Adhesion↗

The organized categories of infant, child, and adult attachment: flexible vs. inflexible attention under attachment-related stress.

From an evolutionary perspective, a central mechanism promoting infant survival is the maintenance of proximity to attachment figures. Consequently attachment figure(s) represent the infant's primary solution to experiences of fear. Aspects of the development of the field of attachment are outlined within this context, beginning with Bowlby's ethological/evolutionary theory, and proceeding to Ainsworth's early descriptions of infant-mother interaction in Uganda and Baltimore. Using a laboratory procedure called the strange situation, Ainsworth identified three organized patterns of infant response to separation from and reunion with the parent. Narratives derived from videotaped strange situation behavior of infants in each category (secure, avoidant, and resistant/ambivalent) are provided, together with a discussion of the prototypical sequelae of each category (e.g., school behavior, and separation-related narratives and drawings at age six). The Adult Attachment Interview (AAI) and the move to the level of representation are also described. AAI transcripts are presently analyzed according to the speaker's capacity to adhere to Grice's maxims of rational cooperative discourse, and three organized AAI categories, or states of mind with respect to attachment, have been identified (secure-autonomous, dismissing, and preoccupied). When the interview is administered to parents who have been seen with their infants in the strange situation, each AAI category has repeatedly been found to predict that infant's strange situation response to that parent. Illustrations of the discourse characteristic of each category are provided, and it is noted that individuals with apparently unfavorable life histories are found to have secure offspring, providing that their history is recounted coherently. Like infant strange situation behavior, differences in adult security as identified through discourse patterning are interpreted in terms of attentional flexibility or inflexibility under attachment-related stress.

Adolescent↗

Attachment forces of ants measured with a centrifuge: better 'wax-runners' have a poorer attachment to a smooth surface.

The symbiotic ant partners of glaucous Macaranga ant-plants show an exceptional capacity to run on the slippery epicuticular wax crystals covering the plant stem without any difficulty. We test the hypothesis that these specialised 'wax-runners' have a general, superior attachment capacity. We compared attachment on a smooth surface for 11 ant species with different wax-running capacities. The maximum force that could be withstood before an ant became detached was quantified using a centrifuge recorded by a high-speed video camera. This technique has the advantage of causing minimum disruption and allows measurements in very small animals. When strong centrifugal forces were applied, the ants showed a conspicuous 'freezing reflex' advantageous to attachment. Attachment forces differed strongly among the ant species investigated. This variation could not be explained by different surface area/weight ratios of smaller and larger ants. Within species, however, detachment force per body weight (F/W) scaled with the predicted value of W(-)(0.33), where W is body weight in newtons. Surprisingly, our results not only disprove the hypothesis that 'wax-runners' generally attach better but also provide evidence for the reverse effect. Superior 'wax-runners' (genera Technomyrmex and Crematogaster) did not cling better to smooth Perspex, but performed significantly worse than closely related congeners that are unable to climb up waxy stems. This suggests an inverse relationship between adaptations to run on wax and to attach to a smooth surface.

Animals↗

Attachment style in the context of clinical and health psychology: a proposal for the assessment of valence, incongruence, and accessibility of attachment representations in various working models.

By combining the Adult Attachment Interview and the Autobiographical Memory Test, a structured interview was developed as a 'quick and dirty' measure for the assessment of attachment representations in clinical settings. The interview intends to assess valence, incongruence, and accessibility of the attachment representations that are organized in various mental models of relationships. In this study the mental models of relationships involving parents and current partner were addressed, as well as the more general model of self in relationships with significant others. Validity was examined in a sample of couples from the general community. Interrelationships between the different models of attachment relationships were strong enough to warrant the conclusion that each contributes to the same construct (style). Correlations with theoretically proposed consequences of insecure attachment style showed salience of specific working models: valence of self and partner (but not of parents) representations, and incongruence of parental representations related to depressive symptomatology, trait anxiety, relational quality, and communication efficiency. Results indicated that assessment of representations in the working models of different attachment relationships might prove useful in clinical settings, as it might enable localization of those representations that are related to the individual's experience of adversity in interpersonal relationships and affect regulation. Methodological shortcomings are discussed in this context.

Adult↗

Attachment relationships among children with aggressive behavior problems: the role of disorganized early attachment patterns.

This article reviews recent attachment-related studies of early aggression. That aggressive behavior toward peers is related to disorganized or controlling patterns of attachment behavior toward parents but not to avoidant or ambivalent patterns. Longitudinal attachment studies indicate that risk factors identified in cross-sectional studies of aggressive school-age children, such as family adversity, parental hostility, parental depression, and child cognitive deficits, are already evident in infancy and predictive of later aggression, before the onset of coercive child behavior. In infancy, these risk factors are associated with disorganized attachment behaviors toward the caregiver characterized by signs of fear or dysphoria, irresolvable conflict between opposing behavioral tendencies, and elevated cortisol levels after separation. Disorganized attachment behaviors, in turn, predict aggression in school-age children with other family factors controlled.

Aggression↗

Attachment, caregiving, and altruism: boosting attachment security increases compassion and helping.

Recent studies based on J. Bowlby's (1969/1982) attachment theory reveal that both dispositional and experimentally enhanced attachment security facilitate cognitive openness and empathy, strengthen self-transcendent values, and foster tolerance of out-group members. Moreover, dispositional attachment security is associated with volunteering to help others in everyday life and to unselfish motives for volunteering. The present article reports 5 experiments, replicated in 2 countries (Israel and the United States), testing the hypothesis that increases in security (accomplished through both implicit and explicit priming techniques) foster compassion and altruistic behavior. The hypothesized effects were consistently obtained, and various alternative explanations were explored and ruled out. Dispositional attachment-related anxiety and avoidance adversely influenced compassion, personal distress, and altruistic behavior in theoretically predictable ways. As expected, attachment security provides a foundation for care-oriented feelings and caregiving behaviors, whereas various forms of insecurity suppress or interfere with compassionate caregiving.

Adolescent↗

The importance of shared environment in infant-father attachment: a behavioral genetic study of the attachment q-sort.

In this first behavior genetic study on infant-father attachment, we estimated genetic and environmental influences on infant-father attachment behaviors and on temperamental dependency, both assessed with the Attachment Q-Sort (AQS; B. E. Vaughn & E. Waters, 1990; E. Waters, 1995). Mothers of mono- and dizygotic twins (N = 56 pairs) sorted the AQS with a focus on the infant's behaviors in the presence of the father. Genetic modeling showed that attachment was largely explained by shared environmental (59%) and unique environmental (41%) factors. For dependency, genetic factors explained 66% of the variance, and unique environmental factors including measurement error explained 34%. Attachment to father appears to be, to a significant degree, a function of the environment that twins share.

Adult↗

The Adult Attachment Interview and questionnaires for attachment style, temperament, and memories of parental behavior.

Relations between Adult Attachment Interview (AAI) outcomes and data from questionnaires on attachment style, temperament, and memories of parental caregiving behavior were investigated to examine theoretical and methodological specificity of the AAI. The participants were 83 mothers of 1-year-olds. No differences between the three AAI classifications (autonomous, dismissing, or preoccupied) were found. Correlations between scales yielded few significant relations, with the exception of strong relations between some AAI scales for experiences and self-reported memories of parental behavior. The self-report questionnaires for attachment style and memories of parental behavior were therefore found to be not suitable for obtaining information about attachment working models as assessed by the AAI. Furthermore, attachment working models appear independent of temperament.

Female↗

Attachment and dysthymia: the contributions of preoccupied attachment and agency of self to depression in women.

The present research evaluated a conceptual model that links preoccupied attachment to dysthymic disorder in women. From an original community sample of 420 women, 129 women were identified with depressive symptomatology as assessed by the Centre for Epidemiological Studies Depression scale (CES-D). Twenty-four of these 129 women were diagnosed as dysthymic disordered according to the Structured Clinical Interview for DSM-III-R-non-patient edition (SCID-NP). Attachment patterns were assessed using the Adult Attachment Projective (AAP). The results indicate that preoccupied attachment was associated with dysthymia. Discussion concerns the processes that may underlie the association between preoccupied attachment and depression, with attention to the possibly complicating factor of unresolved mourning.

Adult↗