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Strains of infectious bronchitis virus on the Delmarva peninsula and in Arkansas.

The constant-virus variable-serum neutralization test in chicken tracheal cultures was used to serotype infectious bronchitis virus (IBV) isolants from the Delmarva peninsula and Arkansas. Of 106 isolants obtained from Delmarva in 1971-1974, 93 were found to be the JMK strain, 5 Massachusetts (Mass), 5 Connecticut (Conn), 2 a new strain designated Maryland (Md) 27, and 1 a new strain designated Md 31. Of 26 isolants obtained from Arkansas between October, 1971, and February, 1973, 17 were Arkansas (Ark) 99, 4 Mass, 1 Conn, and 1 an additional new strain, designated Ark 155. Antisera produced against 2 more isolants each neutralized both Ark 99 and Mass viruses. Antiserum against another isolant neutralized both Ark 155 and Conn viruses. The neutralizing characteristics of the new strains are described.

Animals↗

Pertussis in Arkansas.

Although the morbidity and mortality due to pertussis has declined, 109 infants and children were diagnosed and reported to the Arkansas Department of Health with pertussis in 1990-1994. Case rates in Arkansas over this five year period were lower than national case rates (0.9/100,000 vs. 1.77/100,000; p < 0.0001) while the case fatality rates were similar. Hospitalization, admissions to the intensive care, and deaths in Arkansas were more commonly demonstrated in children < 1 year of age. Although recent data concerning the safety and efficacy of the acellular vaccines is promising, at this time the primary series should still be given with the whole-cell vaccine until more data becomes available.

Arkansas↗

The Master of Public Health program for Arkansas.

A unique Master of Public Health (MPH) program has been operating in Arkansas for the past two years. Developed by the faculty of the School of Public Health and Tropical Medicine at Tulane University Medical Center, it offers health professionals the opportunity to earn the MPH degree over a two-year period while remaining in their positions and communities. Most classes are taught on the campus of the University of Arkansas for Medical Sciences (UAMS) in Little Rock. Several courses have been offered by the faculty of the Health Services Administration program and Department of Biology at the University of Arkansas at Little Rock (UALR).

Adult↗

Identification and partial characterization of Arkansas isolates of chicken anemia virus.

Chickens from both broiler and broiler breeder pullet flocks experiencing symptoms of chicken anemia virus (CAV) infection were first observed at the Poultry Health Research Laboratory at the University of Arkansas in September 1992. Flocks had experienced higher than normal mortality with subcutaneous hemorrhages on the wings, neck, and thorax. Postmortem and histopathologic evaluation revealed thymus and bursal atrophy and lesions consistent with those reported for CAV infection. Because this infection had not previously been observed by Poultry Health Research Laboratory personnel in Arkansas-grown chickens, the establishment of a definitive diagnosis was deemed important. The presence of CAV was established by infecting MSB-1 cells with pooled liver homogenates from groups of 10 specific-pathogen-free chickens that had previously been inoculated in an attempt to experimentally reproduce the disease observed in the field. Cytopathic effects in the infected MSB-1 cells were first evident following the fifth passage. Indirect fluorescent antibody technique identified infected MSB-1 cells following at least five blind passages. To further confirm the presence of CAV, a polymerase chain reaction (PCR) technique was used to amplify a specific portion of the virus genome from infected MSB-1 cells and tissue extracts from several submitted chickens. Sequence analysis of a 186-bp PCR amplification product revealed that the Arkansas isolate was very similar to the Cuxhaven-1 isolate (99.5% sequence identity).

Amino Acid Sequence↗

Prevention of perinatal transmission of HIV in Arkansas.

In 1994, the Centers for Disease Control and Prevention (CDC) recommended zidovudine (ZDV) prophylaxis to reduce perinatal transmission of HIV. Caregivers at the University of Arkansas for Medical Sciences (UAMS) instituted a program of universal voluntary HIV testing of pregnant females combined with maternal education regarding ZDV prophylaxis in October 1994. Since that time, 7 of 39 (18%) infants referred to Arkansas Children's Hospital (ACH) for evaluation of perinatal HIV exposure have been infected compared to 21 of 53 (40%) referred prior to October 1994, (p = 0.042). Unfortunately, of the 39 infants referred to ACH after October 1994, 21 were born to HIV-infected mothers who did not comply with prophylaxis. Fifteen of these mothers were not offered intravenous ZDV during delivery; five have children infected with HIV. These data indicate the need for increased efforts by health officials in Arkansas to institute nationally recommended methods of prevention of perinatal HIV.

Anti-HIV Agents↗

A five-base pair deletion in the sedlin gene causes spondyloepiphyseal dysplasia tarda in a six-generation Arkansas kindred.

A six-generation kindred from Arkansas with X-linked recessive spondyloepiphyseal dysplasia tarda (SEDT) was investigated by genetic linkage and mutation analysis. SEDT had been mapped on the X-chromosome (Xp22.2), and the clinical and radiographic evolution of this kindred had been published. Linkage analysis proved informative for all five polymorphic markers tested, and DXS987 and DXS16 co-segregated with the Arkansas kindred (peak logarithm of the odds scores, 3.54 and 3.36, respectively). Subsequently, dinucleotide deletion in a new gene designated "sedlin" was reported to cause SEDT in three families. In an affected man and obligate carrier woman in the Arkansas kindred, we found a 5-bp deletion in exon 5 of sedlin. The defect causes a frameshift, resulting in eight missense amino acids and premature termination. The 5-bp deletion was then demonstrated to segregate with SEDT in the four living generations, including eight affected males and nine obligate carrier females. Furthermore, the deletion was identified in four females who potentially were heterozygous carriers for SEDT. The mutation was not detected in the two young sons of the consultand (believed to be a carrier because of her subtle radiographic skeletal changes and then shown to have the deletion), but they were too young for x-ray diagnosis Identification of a defect in sedlin in this SEDT kindred enables carrier detection and presymptomatic diagnosis and reveals an important role for this gene in postnatal endochondral bone formation.

Adult↗

Occurrence of dispensing errors and efforts to reduce medication errors at the Central Arkansas Veteran's Healthcare System.

BACKGROUND: Medical errors have received national attention in the past few years, largely due to the Institute of Medicine's (IOM) 1999 report, which found that over one million injuries and nearly 100,000 deaths occur annually in the US as a result of medical errors. PURPOSE: The purpose of this study was to examine the type and severity of dispensing errors reported by pharmacy services at the Central Arkansas Veteran's Healthcare System from October 1997 through September 2001 and to examine the efforts implemented by the Central Arkansas Veteran's Healthcare System to reduce overall medication-related errors. METHODS: Dispensing error reports for the Central Arkansas Veteran's Healthcare System were obtained for October 1997 to September 2001. Dispensing errors were tabulated in the Statistical Package for the Social Sciences (SPSS) according to the pharmacy section, type of error (wrong drug, wrong dose, wrong patient and 'other') and severity of error (minor, significant, major and unrated). Data were explored using descriptive statistics, chi2, independent sample t-tests and Pearson's correlation. Information on error reduction efforts was obtained from pharmacy administrative services. RESULTS: A total of 82 dispensing errors were reported from eight different pharmacy sections for the time period examined. Errors included 31 wrong drugs, 21 wrong doses, 24 wrong patients and six 'other' errors. The number of errors, according to severity, included 29 unrated, 30 minor, 21 significant and two major errors. Both major errors were due to wrong drug selection. In total, the highest number of errors occurred at the North Little Rock Ambulatory Care Pharmacy (39 errors) and the Little Rock Ambulatory Care Pharmacy (24 errors). Wrong drug and wrong dose dispensing errors were not significantly different among the pharmacy sections. Wrong patient selection was significantly different among pharmacy service sections. Wrong patient selection, wrong drug, and wrong dose were all significantly correlated with unrated severity, minor severity, and significant severity. Significant correlations were also found between wrong drug, wrong dose and wrong patient selection. There were no significant correlations between wrong patient selection and major severity, or other errors. chi2 analysis found significant differences in expected frequency among errors for wrong drug, wrong dosage, wrong patient and other errors. Significant differences were also found in expected frequencies between unrated, minor, significant and major errors. DISCUSSION: Although the major dispensing errors were not statistically different according to pharmacy services sections and not significantly correlated with any other categories, they both involved the selection of the wrong drug, which was also the most common error. In contrast, the selection of the wrong patient, the second most common error, was statistically different among pharmacy sections and was significantly correlated with all other dispensing type and severity of error except major severity and other errors. CONCLUSION: Focusing error reduction efforts on selection of the correct drug and correct patient would likely yield the best results in reducing dispensing errors since these errors combined accounted for 55 (67.1%) of the 82 reported errors.

Hospitals, Veterans↗

A SAMPLE SURVEY OF SELECTED AREAS IN AND NEAR LITTLE ROCK, ARKANSAS, TO ASSESS THE PREVALENCE OF ENTAMOEBA HISTOLYTICA.

Laboratory confirmed clinical amoebiasis was diagnosed among more than 50 persons by the University of Arkansas Medical Center from 1965 to 1961. The presence of this number of patients prompted an investigation into the prevalence of Entamoeba histolytica in four selected areas in and around Little Rock, Arkansas. The results of the study, using a sample survey technique, showed that it is possible to have a seemingly large number of clinically significant cases of amoebiasis in a population without a high prevalence of the parasite. Evidence was also obtained of the beneficial effects of improvements in sanitary facilities, as reflected in the lower amoebic prevalence rate in a 0-4-year age-group from one of the four areas surveyed in which a piped, indoor city-water supply and sewage facilities had been installed six years previously.

Amebiasis↗

First-year survival of infants born with congenital heart defects in Arkansas (1993-1998): a survival analysis using registry data.

BACKGROUND: In the United States and other developed nations, birth defects are the leading cause of infant mortality. Congenital heart defects (CHDs) are among the most prevalent and fatal of all birth defects. Here we report the survival probability of infants born with CHDs in Arkansas and examine the impact of multiple malformations on survival. METHODS: Birth and death certificate records were linked to birth defects registry data for infants born with CHDs from January 1993 through December 1998 in Arkansas. Both neonatal and first-year survival probabilities were estimated. These were computed non-parametrically using Kaplan-Meier's product limit method. A Cox proportional-hazards model was used to evaluate the relative importance of additional malformations on survival. RESULTS: A total of 1,983 infants with CHDs were included in this study. The neonatal survival probability for this cohort was 94.0% (95% CI: 93.0%, 95.1%), and the first-year survival probability was 88.2% (95% CI: 86.8%, 89.6%). The presence of hypoplastic left heart syndrome conferred the greatest reduction in survival, whereas infants with pulmonic valve stenosis and infants with ventricular septal defects had the highest first-year survival. Infants with multiple CHDs had decreased survival compared to those with isolated heart defects. Survival was also adversely affected by the presence of congenital abnormalities in other body systems. CONCLUSIONS: Neonatal and first-year survival of infants with CHDs varies by both the type of cardiac malformation and the presence of additional cardiac and non-cardiac malformations. Further work will focus on the effects of maternal and infant characteristics on survival.

Abnormalities, Multiple↗

Traumatic spinal cord injury in Arkansas, 1980 to 1989.

To develop and implement target prevention strategies, we studied the causes of spinal cord injuries in Arkansas and the groups most affected by SCI. Any Arkansas resident with a spinal cord injury who satisfied the state registry criteria was defined as a subject. Transportation-related incidents caused 319 (49.5%) of the SCIs. Males 15 to 24 years of age experienced the highest incidence of SCI because of transportation and sport-related injuries. The rate of SCI from falls was 2.8 times higher for people 65 years of age and older than for people 64 years of age and younger. Minority males 15 to 44 years of age were at the greatest risk of violence-related SCIs. More intervention is needed in the areas of transportation and sport-related causes involving males 15 to 24 years of age, fall-related causes involving the elderly, and violence-related causes involving minority males 15 to 44 years of age.

Adolescent↗

A provisional fish index of biotic integrity for assessing Ouachita Mountains streams in Arkansas, U.S.A.

Multimetric indices are often used to monitor aquatic-resource conditions. We used existing fish-collection data from streams to develop an Index of Biotic Integrity (IBI), which is a multimetric index, for the Ouachita Mountains ecoregion in Arkansas, U.S.A. Each fish-collection site was categorized as reference or non-reference. We examined 62 candidate IBI metrics, and selected 12 non-redundant metrics that differentiated best between reference and non-reference sites. The selected metrics were: Percent (of individuals) as Black Bass; Percent as Benthic Feeders; Percent as Centrarchids; Percent as Cyprinids; Percent as Ictalurids; Percent as Mineral, Site-Prep Spawners; Percent as Mineral, Site-Prep, Parental-Care Spawners; Percent as Simple, Mineral Substrate Spawners; Percent as Miscellaneous, Site-Prep, Parental-Care Spawners; Total Number of Centrarchid Species; Total Number of Arkansas Department of Environmental Quality (ADEQ) Ouachita Mountains Indicator Species; and Total Number of ADEQ Ouachita Mountains Key Species. We standardized each metric to score from 0 to 10 by using linear equations and threshold limits. Some selected IBI metrics had their scoring criteria adjusted to account for watershed size (i.e., stream size). We standardized the IBI to score from 0 to 100. In addition, we determined that our Percent as Black Bass and Percent as Benthic Feeders metrics contributed most to IBI scores in reference conditions, but their contributions decreased with decreasing stream conditions. Reproductive metrics contributed most in degraded stream conditions. Furthermore, we identified relations between IBI metrics and water-quality and land-use variables; some relations were counterintuitive. Unexpected relations may be random observations explained by limited ranges of land-use and water-quality variables. When select water-quality and land-use variables were included in a principal component analysis, a composite Land Use Intensity variable explained most of the model variance. Although the IBI has not been independently validated, the PCA, as well as other superficial analyses, indicated that the IBI should be able to differentiate stream conditions.

Animals↗

Spiders and Borrelia burgdorferi: no evidence of reservoir occurrence in central Arkansas.

BACKGROUND: Although Ixodes ticks are considered the chief vector for Borrelia burgdorferi in the USA, B. burgdorferi has also been identified in mosquitoes, horse flies, and deer flies. We examined the possibility of these organisms being harbored in two species of spider in central Arkansas. METHODS: Ten wolf spiders (Lycosa gulosa) and two brown recluse spiders (Loxosceles reclusa) were collected in central Arkansas during early summer and fixed in formalin. Paraffin-embedded sections of the spiders were examined for spirochetes using the modified Steiner spirochete staining method and examined for B. burgdorferi using immunohistochemistry. RESULTS: All 12 spiders from both species were found to be negative for all spirochetes including B. burgdorferi. CONCLUSIONS: Spiders in our sample appeared not to harbor B. burgdorferi. Further studies utilizing larger sample sizes, more sensitive testing measures, or spiders from an area more endemic with B. burgdorferi may further prove or disprove that spiders are capable of harboring this organism.

Animals↗

Mutant allele frequencies in domestic cat populations in Arkansas and Tennessee.

We conducted surveys of mutant allele frequencies of four cat populations in Arkansas and Tennessee during 2002. Our calculations and analyses support that Southwestern cat populations were relatively more genetically similar to each other than compared to cat populations in other areas of North America. However, the cat population of Fort Smith is slightly different from the other cat populations studied in the Southwestern United States. Although there is a clear significant spatial geographic pattern for many mutant coat allele frequencies in the United States and Canada cat populations (d, l, S, and W), our results revealed that there is not a significant isolation-by-distance model affecting these cat populations. Our data also support the historical migration hypothesis because our calculated allele frequencies were genetically similar to cat populations located in ancestral areas of Europe. Different phenograms, including new European cat genetic profiles, showed that the Southwestern cat populations studied are of a clear British origin. Therefore, migration routes of early Arkansas and Tennessee settlers help explain the similarities of allele frequencies among domestic cat populations.

Animals↗

Impact of immunization against Haemophilus influenzae type b (HIB) on the incidence of HIB meningitis treated at Arkansas Children's Hospital.

The newly available Haemophilus influenzae type b (HIB) protein conjugate vaccines are efficacious among study populations in which a high proportion of infants and children are vaccinated. In this retrospective study, we show the impact of the availability of HIB conjugate vaccines on the incidence of HIB meningitis at Arkansas Children's Hospital (ACH) in Little Rock. The Arkansas State Health Department estimates that only 43% of children in the state younger than 2 years of age have received the appropriate vaccinations. From 1985 through 1987, 27.3 +/- 4 HIB meningitis cases per year were treated at ACH. Although an HIB conjugate vaccine was licensed for 18-month-old children in December 1987, the incidence of HIB meningitis treated at ACH did not decrease significantly; there were 19.0 +/- 2 cases per year from 1988 through 1990. In December 1990, an HIB conjugate vaccine was licensed for use in infants beginning at 2 months of age. From that time through August 1992, there were five cases of HIB meningitis treated at ACH, representing a significant decrease over previous years. Four of these cases occurred in unimmunized infants younger than 6 months of age. The availability of HIB conjugate vaccines for infants has resulted in a dramatic decrease in the number of cases of HIB meningitis treated at ACH, despite a relatively low proportion of infants and children who are receiving vaccination.

Arkansas↗

Prevalence of Salmonella enteritidis in poultry shell eggs in Arkansas.

The objective of this study was to determine whether poultry shell eggs are a major reservoir of Salmonella enteritidis in Arkansas. One hundred dozen commercially purchased shell eggs were cultured for the presence of Salmonella sp. After each dozen eggs was examined, the contents of the 12 eggs were separated from their shells. The contents and the shells were separately pooled and cultured. One dozen of the 100 dozen egg shells cultured were found to be externally contaminated with S heidelberg, while none of the contents of the 100 dozen eggs were found to contain Salmonella organisms. The reevaluation of previously obtained telephone follow-up data on 204 patients with Salmonella infections from 1992-1993 revealed that 30 had consumed raw eggs before their salmonellosis but only one patient was infected with S enteritidis. These data suggest that poultry shell eggs are not a major cause of human illness due to S enteritidis in Arkansas.

Animals↗

Correlates of breastfeeding initiation in southeast Arkansas.

OBJECTIVES: Southeast Arkansas is a primarily rural, low-income area with low breastfeeding rates. Given the demonstrated positive impacts of breastfeeding on a variety of health indicators, it is important to understand and counteract this situation. METHODS: We reviewed the medical records of 1,260 women who delivered infants at the only major hospital in southeastern Arkansas between February 1997 and January 1998 to determine the rate of breastfeeding initiation and to assess associated factors. RESULTS: Only 18% of mothers initiated breastfeeding. Black mothers, unmarried mothers, and those with less than high school education were least likely to breastfeed. Participation in childbirth education classes was positively associated with breastfeeding, but participation in the Supplemental Food Program for Women, Infants, and Children, health care provider, and hospital variables were not predictive in multivariate models. CONCLUSIONS: Breastfeeding promotion programs are clearly needed in this region, and health care providers and the Supplemental Food Program for Women, Infants, and Children can play significant roles.

Adolescent↗

Molecular epidemiologic surveillance of salmonellosis in Arkansas.

OBJECTIVES: Salmonella serotype Newport and Salmonella serotype Typhimurium are the most commonly identified serotypes of Salmonella causing human disease in the state of Arkansas. The purpose of our study was to compare the results of standard and molecular epidemiologic methods of investigating human salmonellosis cases due to Salmonella serotype Newport and Salmonella serotype Typhimurium. METHODS: All isolates of Salmonella serotype Newport and Salmonella serotype Typhimurium collected and submitted to the Arkansas Department of Health between July 1, 1997 and June 30, 1998 were gathered and underwent pulsed-field gel electrophoresis (PFGE). Patients from whom the isolates were collected were contacted and completed a questionnaire. RESULTS: There were 84 patients from whom Salmonella serotype Newport was isolated and 83 from whom Salmonella serotype Typhimurium was isolated during the study period. In the 124 patients (74%) who completed the questionnaire, Salmonella serotype Newport was more likely to be the infecting agent in younger, white, and pet-owning patients (P < 0.05). The use of PFGE confirmed that approximately 20% of the organisms had genetic fingerprint patterns identical to those of at least one other individual in the state. One third of the patients from whom these isolates were obtained were linked by standard epidemiologic methods. CONCLUSIONS: The use of PFGE on our state's most common isolates provides additional confirmation that despite being linked by time of onset and location of residence, the majority of the human salmonellosis cases in our region are still sporadic. Low-level, intermittent transmission of these organisms through environmental contamination and contact with asymptomatically infected individuals would be likely vehicles of transmission in our state. Molecular techniques are important in surveillance systems that investigate human salmonellosis. Eighty-one percent of the Salmonella serotype Newport and 92% of the Salmonella serotype Typhimurium cases that appeared to be outbreak-related based upon time of onset and location were actually found not to be outbreak-related by PFGE. Using techniques such as PFGE will allow for more focused evaluations of potential outbreaks and will save the already limited financial and human resources that would otherwise be spent on investigations that are not warranted.

Adolescent↗

Establishing a statewide mammography database in Arkansas: overcoming the barriers.

To determine patterns of mammography utilization in Arkansas, the Arkansas Mammography Data Collection Project (MDCP) was established. The project's objective was to compile into one database statewide information about mammograms performed. All mammography centers were invited to participate in the project. Many barriers were encountered that were center related, data related, or personnel related; different interventions were implemented for each barrier. At the conclusion of the project, 92 out of 112 centers (82%) participated in the project, creating a database of 157,976 mammography data sets. Identifying and overcoming many of the barriers were crucial steps in the project's success.

Arkansas↗