Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “process mapping”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 703 records · Page 39Linked to original sources

New ICD-10 version of the Charlson comorbidity index predicted in-hospital mortality.

BACKGROUND AND OBJECTIVE: The ICD-9-CM adaptation of the Charlson comorbidity score has been a valuable resource for health services researchers. With the transition into ICD-10 coding worldwide, an ICD-10 version of the Deyo adaptation was developed and validated using population-based hospital data from Victoria, Australia. METHODS: The algorithm was translated from ICD-9-CM into ICD-10-AM (Australian modification) in a multistep process. After a mapping algorithm was used to develop an initial translation, these codes were manually examined by the coding experts and a general physician for face validity. Because the ICD-10 system is country specific, our goal was to keep many of the translated code at the three-digit level for generalizability of the new index. RESULTS: There appears to be little difference in the distribution of the Charlson Index score between the two versions. A strong association between increasing index scores and mortality exists: the area under the ROC curve is 0.865 for the last year using the ICD-9-CM version and remains high, at 0.855, for the ICD-10 version. CONCLUSION: This work represents the first rigorous adaptation of the Charlson comorbidity index for use with ICD-10 data. In comparison with a well-established ICD-9-CM coding algorithm, it yields closely similar prevalence and prognosis information by comorbidity category.

Algorithms↗

Body-surface potential mapping to aid ablation of scar-related ventricular tachycardia.

We investigated whether body-surface potential mapping (BSPM) during catheter ablation of scar-related ventricular tachycardia (VT) could assist with the identification of VT exit sites. The study population consisted of 9 patients who underwent catheter ablation for VT, among whom 12 induced VTs with known exit sites were identified by entrainment criteria, pace mapping, or site of successful ablation. Paced activation was initiated at various intracardiac sites (20 +/- 4 sites per patient, a total of 180) documented by nonfluoroscopic electroanatomic mapping. During all episodes of VT and pacing, patients had a 120-lead electrocardiogram recorded, and we analyzed these electrocardiographic data--by means of a similarity coefficient (SC) calculated over 100 milliseconds after the initiation of depolarization--to assess the similarity between the BSPM sequences occurring during VTs and those induced by pacing. Based on 245 observations, the relationship between the SC and the distance of the pacing site from the VT exit site was then obtained for each individual VT by linear regression analysis: the distance D (in millimeters) from the VT exit site was related to SC by the regression equation D = slope (1 - SC2) + intercept. The parameters in this equation varied widely for the 12 VTs, but, in general, the nearer the pacing site was to the exit site, the better the goodness of match. This suggests that, although there is no universally applicable relationship between D and SC, BSPM could provide a useful adjunct to standard pace mapping, although additional processing--namely, an inverse calculation of epicardial potentials/isochrones--may be needed to reliably identify VT exit sites from body-surface electrocardiograms.

Aged↗

Structure of the mature P3-virus particle complex of cauliflower mosaic virus revealed by cryo-electron microscopy.

The cauliflower mosaic virus (CaMV) has an icosahedral capsid composed of the viral protein P4. The viral product P3 is a multifunctional protein closely associated with the virus particle within host cells. The best-characterized function of P3 is its implication in CaMV plant-to-plant transmission by aphid vectors, involving a P3-virion complex. In this transmission process, the viral protein P2 attaches to virion-bound P3, and creates a molecular bridge between the virus and a putative receptor in the aphid's stylets. Recently, the virion-bound P3 has been suggested to participate in cell-to-cell or long-distance movement of CaMV within the host plant. Thus, as new data accumulate, the importance of the P3-virion complex during the virus life-cycle is becoming more and more evident. To provide a first insight into the knowledge of the transmission process of the virus, we determined the 3D structures of native and P3-decorated virions by cryo-electron microscopy and computer image processing. By difference mapping and biochemical analysis, we show that P3 forms a network around the capsomers and we propose a structural model for the binding of P3 to CaMV capsid in which its C terminus is anchored deeply in the inner shell of the virion, while the N-terminal extremity is facing out of the CaMV capsid, forming dimers by coiled-coil interactions. Our results combined with existing data reinforce the hypothesis that this coiled-coil N-terminal region of P3 could coordinate several functions during the virus life-cycle, such as cell-to-cell movement and aphid-transmission.

Caulimovirus↗

Trials and tribulations: a primer on successfully navigating the waters of the Food and Drug Administration.

Conducting clinical trials in a regulated environment constitutes unfamiliar territory for most physicians. There exists no formal training in this subject, so many clinicians lack even a basic understanding of the procedures required by the Federal Food, Drug, and Cosmetic Act and their implications vis-à-vis clinical practice. The authors distill drug regulation to relevant applications and elucidate the rationale and procedures for submitting an Investigational New Drug application by providing a user-friendly road map of the process.

Clinical Trials as Topic↗

Identifying stakeholder behaviors for competency-based pharmacy education: A stage 1 behavior change wheel analysis.

INTRODUCTION/OBJECTIVES: Competency-Based Pharmacy Education (CBPE) is a strategic priority for preparing graduates to meet evolving healthcare needs. However, efforts to implement CBPE can stall due to behavioral challenges among faculty, administrators, preceptors, and learners. This study aimed to apply Stage 1 of the Behavior Change Wheel (BCW) to identify stakeholder-specific behaviors and associated determinants needed to implement the five core components of CBPE. METHODS: A multi-method approach grounded in the BCW, the Capability, Opportunity, Motivation - Behavior (COM-B) model, and the Theoretical Domains Framework (TDF) was used. Data were gathered through (1) targeted literature review; (2) structured focus groups with competency-based education experts and pharmacy education stakeholders; and (3) an iterative consensus process. Behaviors were mapped to the five CBPE components: (1) defined competencies, (2) developmental progression, (3) tailored instruction, (4) authentic experiential learning, and (5) programmatic assessment, and then mapped to COM-B and TDF constructs. RESULTS: Over fifty stakeholder-specific behaviors were identified and specified across the CBPE framework. This revealed shared barriers such as limited instructional design knowledge (psychological capability), insufficient assessment of infrastructure (physical opportunity), and misaligned professional identity (reflective motivation). Key TDF domains included knowledge, environmental context, beliefs about capabilities, and professional roles. The behavioral problem statements, specifications, and determinants were identified to support future intervention planning. CONCLUSION: This Stage 1 analysis provides a behaviorally grounded foundation for CBPE implementation by identifying stakeholder behaviors and conditions that enable change. These findings will inform the development of readiness-to-change assessments and targeted interventions (BCW Stages 2 and 3), supporting scalable and sustainable CBPE transformation in pharmacy education.

Education, Pharmacy↗

Atrioventricular valve development during late embryonic and postnatal stages involves condensation and extracellular matrix remodeling.

Although the signaling molecules regulating the early stages of valvular development have been well described, little is known on the late steps leading to mature fibrous leaflets. We hypothesized that atrioventricular (AV) valve development continues after birth to adjust to the postnatal maturation of the heart. By doing a systematic analysis of the AV valves of mice from embryonic day (E) 15.5 to 8 weeks old, we identified key developmental steps that map the maturation process of embryonic cushion-like leaflets into adult stress-resistant valves. Condensation of the mesenchymal cells occurred between E15.5 and E18.5 and was accompanied by increased cellular proliferation and adhesion. Cellular proliferation also contributed transiently to the concomitant elongation of the leaflets. Patterning of the extracellular matrix (ECM) proteins along the AV axis was achieved 1 week after birth, with the differentiation of two reciprocal structural regions, glycosaminoglycans and versican at the atrial side, and densely packed collagen fibers at the ventricular side. Formation and remodeling of the nodular thickenings at the closure points of the leaflets occurred between N4.5 and N11.5. In conclusion, AV valve development during late embryonic and postnatal stages includes condensation, elongation, formation of nodular thickenings, and remodeling of tension-resistant ECM proteins.

Animals↗

Prehension with the ipsilesional hand after unilateral brain damage.

Sensorimotor deficits in the hand ipsilateral to a brain lesion have been reported in different motor tasks. We evaluated performance of the ipsilesional hand in 12 patients with either left (LBD) or right brain damage (RBD) by kinematic analysis in order to precisely characterize possible deficits in the two components of prehension (transport and grasp). Both patient groups exhibited performance deficits in the main kinematic parameters, e.g., reduced velocity of the transport component and prolonged movement time. However, while LBD patients showed a more general slowing, RBD patients prolonged in particular the last phase of the movement toward the object. We suggest that relevant visuospatial representations and the adequate mapping of motor processes may be impaired after RBD. In contrast, LBD caused a more unspecific disturbance pattern, supporting the view that the precise parameterization of motor programs is impaired. Maximum grip aperture was normal in both patient groups. However, since aperture could be biased by slowed movement, the notion that the grasp component was preserved remains speculative. The patient's ability to scale the maximum velocity of the transport component to adapt to changes in movement amplitude and to scale the maximum hand aperture of the grasp component to adapt to object size was preserved in both groups. Thus both hemispheres can have competence for this scaling mechanism.

Adult↗

On the receptive prosodic loss in Parkinson's disease.

To comprehensively explore how the processing of linguistic and affective prosodic cues is affected by idiopathic Parkinson's disease (PD), a battery of receptive tests was presented to eleven PD patients without intellectual or language impairment and eleven control subjects (NC) matched for age, gender, and educational attainment. Receptive abilities for both low-level (discrimination) and higher-level (identification) prosodic processing were explored; moreover, the identification of prosodic feature was tested at both the lexical level (phonemic stress perception) and over the sentential domain (prosodic pattern identification). The results obtained demonstrated a general reduction in the ability of the PD patients to identify the linguistic- and affective-prosodic meaning of utterances relative to NC subjects, without a concurrent loss in the ability to perceive phonemic stress contrasts or discriminate prosodic patterns. However, the qualitative pattern of the PD and NC groups' performance across the various identification conditions tested was remarkably uniform, indicating that only quantitative differences in comprehension abilities may have characterized the two groups. It is hypothesized that the basal ganglia form part of a functional network dedicated to prosodic processing (Blonder et al., 1989) and that the processes required to map prosodic features onto their communicative representations at the sentence level are rendered less efficient by the degenerative course of PD.

Aged↗

Regional specification during embryogenesis in Rhynchonelliform brachiopods.

Fate maps have been constructed for embryos of Hemithiris and Terebratulina, representatives of two orders in the class Rhynchonellata that have been separated since the middle Ordovician. These fate maps are identical. The animal region of the egg forms the ectodermal covering of the apical lobe and the vegetal region is the site of gastrulation; the vegetal region forms the ectoderm of the ventral and posterior regions of the larva, endoderm and mesoderm. The cells that make up the animal region shift 90 degrees with respect to the vegetal pole during gastrulation. The timing and mode of regional specification in these two species are also identical. In each case, the animal region of the unfertilized egg has the intrinsic ability to form apical lobe ectoderm, while the vegetal region has the ability to form a normal larva. During embryogenesis, the vegetal region interacts with the animal region to suppress apical tuft differentiation in the apical lobe and to promote mantle lobe ectodermal differentiation, while the ability of the vegetal half to regulate by forming apical lobe structures is lost. The plane of bilateral symmetry of the larva begins to be set up between the late blastula and early gastrula stage. The fate maps and the processes of regional specification are compared in the four subphyla that make up the Brachiopoda and used to test a developmental model that provides an explanation for the variety of different body plans generated during the Cambrian.

Alkaline Phosphatase↗

Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei.

The start site of the A-type ribosomal RNA transcription units of the rodent malaria parasite, Plasmodium berghei, has been identified. The two A-type units cannot be distinguished within the transcription unit, yet exist as single copies on different chromosomes. Gene transcription initiates 820 bp upstream of the A-type small subunit (SSU) ribosomal gene and two major processing sites were mapped 610 and 611 nucleotides upstream of the SSU in the external transcribed spacer region. Surprisingly the nucleotide sequence of the DNA region containing the putative ribosomal promoter lacked repetitive DNA sequences typical of ribosomal promoters. This region was further analysed by computer using programs designed to reveal sequence-dependent structural features. Comparison of DNA curvature, duplex stability and pattern of twist angle variation revealed a striking degree of conservation between the ribosomal promoters from Plasmodium and other eukaryotes.

Animals↗

Lateralized ultradian rhythms in time and space: a chronobiological field study in Kenyan Maasai.

Lateralized ultradian rhythms oscillating separately in the right and left hemispheres of the human brain can be monitored by variations in the tactile discrimination of either hand. A previous study in male German subjects has shown that the tactile error rates determined for the right and left hands oscillate with significantly different periodicities. In the present study, a group of Kenyan Maasai shepherds was tested while the subjects were leading herds on daily feeding routes. The Maasai exhibit considerable ultradian rhythms of about 2 hours in tactile error rates of either hand, but in contrast to the German subjects there is no significant difference between the right and left side. While an individual is en route, his hemispheres proceed through alternating states in matching segments of the path. Ultradian rhythms thus 'scan' not only the time of day but also the space, and might provide an intrinsic time-frame for neuronal processes of cognitive mapping.

Adolescent↗

Identification of pigments in paint cross sections by reflection visible light imaging microspectroscopy.

A setup for reflection visible light imaging microspectroscopy (VIS-imaging) as well as its evaluation and application is described and tested. The spatial resolution of the system is approximately 1 microm at a spectral resolution of 4 nm. The optical contrast between different colored particles in the surface of a sample is optimized with a new image processing method for mapping of the distribution of the identified pigment particles. The potential of VIS-imaging in the study of paint cross sections obtained from paintings is explored. Spectra obtained from pigment particles in these cross sections result in classification or identification of several pigments. The investigated paint samples are challenging test cases, as they contain several colored materials with a very fine distribution. VIS-imaging can identify and map the most common traditional blue pigments, i.e., smalt, azurite, ultramarine, and indigo in 17th century oil paintings. Smalt can be identified even after complete discoloration. VIS-imaging analysis assisted in the identification and mapping of modern synthetic red and yellow pigments in a 20th century painting.

Journal Article↗

A new method for the preparation of silyl enol ethers from carbonyl compounds and (trimethylsilyl)diazomethane in a regiospecific and highly stereoselective manner.

The reaction of lithium (trimethylsilyl)diazomethane with aldehydes and ketones has been investigated, and it has been found that quenching at low temperature with MeOH followed by addition of Rh2(OAc)4 gave silyl enol ethers in high yields. Quenching with other electrophiles (e.g., deuterium, MeI) gave terminal and substituted silyl enol ethers with complete control over regio- and stereochemistry. The mechanism of this novel process has been mapped out through a combination of deuterium labeling, ReactIR, and isolation of reaction intermediates.

Journal Article↗

A CASSCF/MR-CI study toward the understanding of wavelength-dependent and geometrically memorized photodissociation of formic acid.

The S(0), T(1), and S(1) potential energy surfaces for the HCOOH dissociation and isomerization processes have been mapped with different ab initio methods. The wavelength-dependent mechanism for the HCOOH dissociation was elucidated through the computed potential energy surfaces and the surface crossing points. The HCOOH molecules in S(1) by excitation at 248 nm mainly decay to the ground state via the S(0) and S(1) vibronic interaction, followed by molecular eliminations in the ground state. The S(1) direct dissociation to HCO((2)A') + OH((2)Pi) is the dominant pathway upon photoexcitation at 240-210 nm. Meanwhile, there is a slight probability that the system relaxes to the ground state via the S(0) and S(1) vibronic interaction at these wavelengths. After irradiation of HCOOH at 193 nm, the S(1) direct dissociation into HCO((2)A') + OH((2)Pi) is energetically the most favorable pathway. In view of high IC efficiency at the S(0)/S(1) conical crossing, the S(1) --> S(0) internal conversion via the S(0)/S(1) point can occur with considerable efficiency. In addition, the S(1) isomerization probably plays a dominant role in the partially conformational memory of the HCOOH photodissociation, which has been discussed in detail.

Journal Article↗

Catheter ablation to suppress atrial fibrillation: evolution of technique at a single center.

BACKGROUND: Catheter ablation of atrial fibrillation is a rapidly evolving art. There is currently no consensus as to optimal methodology. We report a sequential experience, during which three distinct ablation techniques were utilized. METHODS: A cohort of 112 patients in whom atrial fibrillation onset was precipitated by ectopy arising solely from myocardium investing the pulmonary veins underwent catheter ablation using one of three techniques: focal (Group A); individual vein encircling (Group B); vestibule encircling (Group C). In each group, non-fluoroscopic guidance of the mapping and ablation process was provided by the tandem use of CARTO and intracardiac echocardiography. RESULTS: In all groups, endocardial topography was complex, and ablation electrode-endocardial contact was often unstable. Maximal electrode temperatures were low, despite frequent echocardiographic evidence of myocardial boiling. A significant progression in the number of radiofrequency energy applications between groups A and C was observed. Although acute suppression of atrial fibrillation inducibility was observed uniformly, chronic suppression rates were significantly different: Group A = 47%; Group B; 69%; Group C = 87%. CONCLUSIONS: In this non-concurrent cohort, the rate of chronic suppression of atrial fibrillation correlated with the amount of myocardium ablated or electrically isolated. Prospective data will be required to further evaluate whether "more is better."

Adult↗

Evidence against a dedicated system for word learning in children.

Children can learn aspects of the meaning of a new word on the basis of only a few incidental exposures and can retain this knowledge for a long period-a process dubbed 'fast mapping". It is often maintained that fast mapping is the result of a dedicated language mechanism, but it is possible that this same capacity might apply in domains other than language learning. Here we present two experiments in which three- and four-year-old children and adults were taught a novel name and a novel fact about an object, and were tested on their retention immediately, after a 1-week delay or after a 1-month delay. Our findings show that fast mapping is not limited to word learning, suggesting that the capacity to learn and retain new words is the result of learning and memory abilities that are not specific to language.

Adult↗

Genetic association studies in complex disease: disentangling additional predisposing loci from associated neutral loci using a constrained - permutation approach.

In the process of genetically mapping a complex disease, the question may arise whether a certain polymorphism is the only causal variant in a region. A number of methods can answer this question, but unfortunately these methods are optimal for bi-allelic loci only. We wanted to develop a method that is more suited for multi-allelic loci, such as microsatellite markers. We propose the Additional Disease Loci Test (ADLT): the alleles at an additional locus are permuted within the subsample of haplotypes that have identical alleles at the predisposing locus. The hypothesis being tested is, whether the predisposing locus is the sole factor predisposing to the trait that is in LD with the additional locus under study. We applied ADLT to simulated datasets and a published dataset on Type 1 Diabetes, genotyped for microsatellite markers in the HLA-region. The method showed the expected number of false-positive results in the absence of additional loci, but proved to be more powerful than existing methods in the presence of additional disease loci. ADLT was especially superior in datasets with less LD or with multiple predisposing alleles. We conclude that the ADLT can be useful in identifying additional disease loci.

Diabetes Mellitus, Type 1↗

Direct observation of thymine dimer repair in DNA by photolyase.

Photolyase uses light energy to split UV-induced cyclobutane dimers in damaged DNA, but its molecular mechanism has never been directly revealed. Here, we report the direct mapping of catalytic processes through femtosecond synchronization of the enzymatic dynamics with the repair function. We observed direct electron transfer from the excited flavin cofactor to the dimer in 170 ps and back electron transfer from the repaired thymines in 560 ps. Both reactions are strongly modulated by active-site solvation to achieve maximum repair efficiency. These results show that the photocycle of DNA repair by photolyase is through a radical mechanism and completed on subnanosecond time scale at the dynamic active site, with no net change in the redox state of the flavin cofactor.

Binding Sites↗