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At least 703 records · Page 39Linked to original sources
PubMed1995

Hucolin, a new corticosteroid-binding protein from human plasma with structural similarities to ficolins, transforming growth factor-beta 1-binding proteins.

In order to study the cortisol-binding factors in blood, human plasma was applied to a 11 beta-hydroxy-3-oxo-4-androstene-17 beta-carboxyaminoethylamine-(HACA) 1,4-butanediol diglycidyl ether-Sepharose column. Elution of the column with cortisol buffer produced two protein peaks, the minor peak yielded a protein complex of molecular weight approximately 200 kDa, subsequently termed hucolin. SDS-PAGE analysis under reducing and non-reducing conditions revealed hucolin was a disulphide-linked complex of 35-kDa and 75-kDa subunits. Twenty-five amino acid residues of the N-terminus of the 35-kDa subunit were determined and homology searches revealed an 88% sequence identity with the N-terminal region of beta-ficolin, a transforming growth factor-beta 1 (TGF-beta 1)-binding protein purified from porcine uterus.

Amino Acid Sequence↗
PubMed1984

Physiologic properties of steroid hormone-binding proteins in avian blood.

The physiologic properties of steroid hormone-binding proteins have been partially characterized in plasmas of 23 avian species (8 orders and 12 families). A specific sex hormone-binding protein (SBP) with high affinity and low capacity, as found in amphibians and some reptiles and mammals, could not be identified in any of the avian species investigated. In addition SBP appeared to be totally absent in the blood of posthatching and juvenile stages of two species, and in the embryonic blood of three species. For birds it is concluded that sex steroid hormones circulate bound to plasma albumins with low affinity (Kd = 10(-5) mol/liter) and very high capacity. There are two protein-binding systems for corticosterone in the blood of all species investigated, including samples collected from embryos. One is saturable and specific with low capacity (10(-8) -10(-9) mol/liter) and high affinity (Kd = 10(-7) -10(-9) mol/liter), whereas the other is a nonspecific component with very high capacity and low affinity (Kd = 10(-5) mol/liter). Specificity studies show that the high-affinity binding system for corticosterone also binds progesterone with virtually identical affinity, whereas testosterone and estradiol are bound with lower affinity. These data suggest that the high-affinity binding protein in avian blood has physiologic properties similar to mammalian corticosteroid-binding globulin (CBP).

Animals↗
PubMed1993

Free and protein-bound progesterone during normal and luteal phase defective cycles.

OBJECTIVES: New methods for the measurement of the percent free (%FreeP) and percent corticosteroid binding globulin-bound (%CBG-B) fractions of plasma progesterone (P) are described. METHODS: Modifications of previously reported equilibrium dialysis and charcoal adsorption assays were used. P binding was analyzed in single midluteal samples of 16 infertile women with endometrial biopsy proven luteal phase defect (LPD) and 10 infertile women with a normal luteal phase. RESULTS: %FreeP, %CBG-B, percent nonspecific protein-bound P (%NSP), total P and total plasma estradiol (E2) were measured during the normal ovulatory cycles of 5 women. The mean follicular phase %FreeP was found to be lower than in the luteal phase due to a decline in the late follicular phase. This decline correlated with the preovulatory rise in E2. The mean absolute concentration of free P (FreeP) rose significantly in the luteal phase, positively correlating with total P in both phases. Mean %CBG-B and %NSP were unchanged throughout the cycle. P was lower in LPD vs. normal cycles, but %FreeP and %CBG-B were not different. E2 highly correlated with P but not with %FreeP and %CBG-B. CONCLUSIONS: In normal cycles a small but significant decrease in %FreeP occurs in the late follicular phase and is associated with increasing E2. Changes in total P during the ovulatory cycle reflect changes in the concentration of bioactive P. There were no data to support an association of LPD with changes in the bioactive fraction of P.

Adult↗
PubMed1990

Hormonal influences on the relationships between body fatness, body fat distribution, lipids, lipoproteins, glucose and blood pressure in French working women.

The independent associations between overall obesity, body fat distribution, lipids, lipoproteins, glucose, blood pressure and some hormonal factors (sex hormone-binding globulin (SHBG), corticosteroid binding globulin (CBG) and fasting insulin) were cross-sectionally examined in 205 French working women. After adjustment for age, overall adiposity assessed by body mass index (BMI) was significantly associated with most metabolic parameters, whereas regional adiposity assessed by the waist-hip ratio (WHR) was significantly associated only with triglyceride, systolic and diastolic blood pressure. Blood pressure, glucose but not triglyceride, were also negatively and significantly correlated with SHBG and positively with fasting insulin. Negative independent associations were found between SHBG and both BMI and WHR, whereas CBG was positively associated only with WHR. Fasting insulin was no longer related to WHR after adjustment for BMI. After controlling for the effect of SHBG or insulin, the associations between triglyceride, blood pressure and both BMI and WHR were not substantially modified. After adjustment for BMI and WHR, fasting insulin was independently associated with both HDL cholesterol and diastolic blood pressure. In conclusion, in these French women, hormonal factors under study appeared to have little influence on the relationships between body fatness, body fat distribution, metabolic variables and blood pressure.

Adipose Tissue↗