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Shorebird use of coastal wetland and barrier island habitat in the Gulf of Mexico.

The Gulf Coast contains some of the most important shorebird habitats in North America. This area encompasses a diverse mixture of estuarine and barrier island habitats with varying amounts of freshwater swamps and marshes, bottomland hardwood forests, and coastal prairie that has been largely altered for rice and crawfish production, temporary ponds, and river floodplain habitat. For the purposes of this review, discussion is confined to general patterns of shorebird abundance, distribution, and macro- and microhabitat use in natural coastal, estuarine, and barrier island habitats on the Gulf of Mexico Coast. The following geographic regions are considered: Northwestern Gulf (Rio Grande to Louisiana-Mississippi border), Northeastern Gulf (Mississippi to Florida Keys), and Mexico (Rio Grande to Cabo Catoche [Yucatan Strait]). Wintering and migrating shorebirds are most abundant along the Gulf Coast in Texas and Tamaulipas, particularly the Laguna Madre ecosystem. Other important areas are the Southwest Coast region of Florida and the area between Laguna Terminos and Puerto Progresso in Mexico. In general, relative abundances of shorebirds increase from north to south, and decrease south of the Tropic of Cancer (23 degrees 27' N). Based on bimonthly maximum counts within 5 latitudinal bands, the region between 25-30 degrees N is used most heavily by wintering and spring migrating birds. Non-vegetated coastal wetland habitats associated with bays, inlets and lagoons, particularly tidal flats, and sandy beaches are the habitats that appear to be favored by wintering and migrating shorebirds. In general, these habitats tend to occur as habitat complexes that allow for movement between them in relation to tidal flooding of bay-shore habitats. This relationship is particularly important to Piping Plover and may be important to others. Although vegetated habitats are used by some species, they do not appear to attract large numbers of birds. This habitat is most widespread between the Texas-Louisiana border and the Florida Panhandle region, but it has not been studied extensively. Shorebird abundance and habitat use in this area need to be addressed.

Animal Migration↗

[Certain aspects of interactions between cohabiting sibling species Microtus arvalis and M. rossiaemeridionalis].

Some aspects of interspecific interactions between sibling species of common vole were studied at the Chernogolovka Scientific Research Station (Severtsov Institute for Problems of Ecology and Evolution, Russian Academy of Sciences) from 1992 to 2001. In the marking plot and indoors, East European vole demonstrated high tolerance to the anthropogenic stress considering that it was the most abundant species in the studied area transformed by human economic activity and that it used buildings as temporary shelters. When cohabiting the marking plot, common and East European voles demonstrated different territorial distribution and circadian activity, which can be considered as an adaptation to avoiding competitive interactions of ecologically similar species.

Adaptation, Biological↗

Defective neutrophil motility in children with measles.

The random migration and chemotactic ability of neutrophils from ten patients with uncomplicated measles was found to be grossly impaired when compared to a group of normal children. Chemotaxis to endotoxin activated serum and to hydrolysed casein was markedly depressed, but serum from measles patients, when activated by endotoxin, generated and chemotactic activity and did not contain leukotactic inhibitors. The defect in neutrophil motility was confirmed in vivo by abnormal Rebuck skin windows. The defect was temporary, and recovery of normal chemotaxis was observed by the eleventh day after the onset of the rash.

Cell Movement↗

Silicone stents in the management of benign tracheobronchial stenoses. Tolerance and early results in 63 patients.

STUDY OBJECTIVE: To assess tolerance and early results of Dumon silicone stents inserted in patients with nonneoplastic airway obstruction. DESIGN: Tracheobronchial stenting for palliative or curative restoration of airway narrowing has been evaluated in a retrospective study. SETTING: Tertiary-referral teaching hospital. PATIENTS: Between May 1991 and September 1994, 64 patients with a mean age of 52 years had endobronchial silicone stent insertion for benign tracheal stenosis (82% secondary to intubation or tracheostomy injury). Lesions were pure fibrous stenosis in 25 patients and fibroinflammatory stenosis in 38. Prostheses were used for temporary stenting of the airway during 18 months in 48 patients in whom cure was expected and as a procedure for palliation in the remaining 15 patients. INTERVENTIONS: In all cases, the Dumon tracheobronchial stent was implanted with the rigid bronchoscope under general anesthesia. RESULTS: Five patients died (four from unrelated causes); one was due to hypersecretion and airway obstruction at the time of an emergency tracheostomy 20 days after stent insertion. Complications included migration of prostheses in 11 (17.5%) patients, granuloma formation in 4 (6.3%) patients, and airway obstruction due to heavy secretion in 4 (6.3%). In 48 patients who received silicone stents with curative expectations, removal of the device was accomplished in 21 patients. Therapy proved successful in 17 patients with a mean follow-up of 259+/- 173 days and stenosis recurred in 4. In 16 patients, stents still remain for a mean period of 364+/-119 days. In the series of 15 patients in whom silicon stents were implanted for palliation, prostheses were placed permanently in 11 with a mean follow-up of 486+/-260 days. In the remaining four patients with tracheostomy, silicone stents were used after inability to expand the upper limb of the T-tube (two patients) or placed above the tracheostomy stoma to maintain laryngotracheal patency and preserve phonation when a T-tube was poorly tolerated (two patients). CONCLUSIONS: Silicone tracheobronchial stents are effective in the maintenance of airway patency and are associated with good tolerance and infrequent complications that are rarely life-threatening.

Adolescent↗

Indications for and complications of mitomycin-C in pterygium surgery.

BACKGROUND AND OBJECTIVE: The purposes of this study are to examine the complications and results of a novel approach to the use of mitomycin-C in pterygium surgery; to identify the lowest effective dosage required to prevent recurrence; and to select those high-risk pterygia that could benefit from mitomycin-C use. PATIENTS AND METHODS: One hundred thirty-five eyes of 128 patients considered at high risk for recurrence of pterygium were treated with a single, intraoperative, subconjunctival injection of mitomycin-C at the site of excision. Three different dosages-0.2, 0.1, and 0.05 ml-at a strength of 0.5 mg/ml were used. The eyes were observed for 10 months to 3 years. RESULTS: There were only two recurrences (1.5%); both occurred in eyes that had undergone previous pterygium surgery. One recurrence happened with the 0.2-ml dosage, and the other with the 0.1-ml dosage. These recurrences occurred in association with early wound dehiscence, which essentially became a bare sclera susceptible to pterygium regrowth. Complications included temporary and prolonged discomfort, tearing, hyperemia, subconjunctival hemorrhage, wound dehiscence, and pigment accumulation. No vision-threatening side effects were noted. CONCLUSIONS: The persistence and intensity of discomfort noted at the higher dosage has prompted these authors to suggest that only high-risk pterygia should receive mitomycin-C. Conjunctival flaps should always be used to cover the sclera up to the limbus and to localize the mitomycin-C to prevent its migration. A single dose of not more than 0.05 ml at a strength of 0.5 mg/ml subconjunctivally gives the same results as multiple drops, but with far less morbidity.

Adolescent↗

Proanthocyanidin: a natural crosslinking reagent for stabilizing collagen matrices.

While attempting to find a suitable crosslinking reagent for biopolymers, a naturally occurring proanthocyanidin (PA) obtained from grape seeds was selected to fix biological tissues. The cytotoxicity and crosslinking rate, reflected by the in vitro and in vivo degradation of fixed matrices has been studied. The shrinkage temperature of the fixed bovine pericardium increased from 66 to 86 degrees C. A cytotoxicity assay using fibroblast cultures revealed that PA is approximately 120 times less toxic than glutaraldehyde (GA), a currently used tissue stabilizer. In vitro degradation studies showed that fixed tissue was resistant to digestion by bacterial collagenase. Crosslinks between PA and tissues can be stabilized by decreasing the dielectric constant of the solution during storage. After subcutaneous implantation for periods ranging between 3 and 6 weeks, we found no apparent degradation of the GA- or PA-fixed tissues, whereas fresh tissue controls rapidly disintegrated. Beyond 6 weeks PA crosslinks began to degrade. More fibroblasts migrated and proliferated inside the PA-fixed implants compared with GA counterparts. Tissues crosslinked with PA manifested an enhanced collagen expression and deposition and did not calcify after implantation. GA, on the other hand, even after thorough rinsing continued to be cytotoxic, inhibited collagen synthesis and encouraged dystrophic calcification. Collagen matrices crosslinked with PA are expected to be of value in the design of matrices that will encourage cell ingrowth and proliferation, which are temporary in nature, and that are intended to regenerate or replace missing tissues, which can delay the biogradation of collagen. As such they should be of significant value in the emerging field of tissue engineering.

3T3 Cells↗

[Prospects of endothelial dysfunction reversion in patients with congestive heart failure].

The aim of the study was to investigate the effect of angiotensin-converting enzyme inhibitor enalapril and non-peptide blocker of AT1 receptors losartan on endothelial function of the shoulder artery in patients with congestive cardiac insufficiency. The examination covered 96 patients (mean age 46.71 +/- 4.13) with stable effort angina (functional class II-III) and circulatory insufficiency (NYHA functional class II-III) having end-diastolic left-ventricular volume > 160 ml, left ventricular ejection fraction < 35%, sinus rhythm, cardiothoracal index > 0.55 units. Patients with fibrillar tachyarrhythmia, high grade blocks, pacemaker migration, artificial pacemaker, myocardial infarction were not included in the trial. The patients were randomized into 3 groups 32 patients each. In addition to basic therapy patients of group 1 received long-acting nitrates, digoxin, aspirin and furosemide; group 2--enalapril in daily dose 10 mg; group 3--losartan in daily dose 25 mg. A course of treatment lasted 12 weeks. Endothelial function was assessed by high resolution echography, dopplerography performed before and after temporary occlusion of the shoulder artery and sublingual nitroglycerin. In patients with cardiac insufficiency, accelerated blood flow in the shoulder artery after its temporary occlusion promoted realization of the vasoconstrictory reaction. This was verified as endothelial dysfunction. In the course of the treatment all the patients achieved insignificant increase of the shoulder artery initial diameter. After sublingual intake of nitroglycerin vasodilation was also insignificant. 12-week enalapril and losartan prevented vasoconstriction in the shoulder artery in response to quicker circulation following arterial occlusion. However, higher maximal flow speed did not correspond to the increment in the artery diameter after the occlusion in any group. The flow-induced vasodilation was more pronounced in the enalapril group. Losartan group had a trend to an increase in the inner diameter of the shoulder artery. It is shown that enalapril and losartan in congestive cardiac insufficiency improves endothelium-dependent vasodilation caused by nitroglycerin. Enalapril demonstrated stronger ability than losartan to reverse endothelial dysfunction in patients with cardiac insufficiency resultant from ischemic heart disease.

Angina Pectoris↗

Endoscopic stenting in the management of biliary stones.

INTRODUCTION: Endoscopic sphincterotomy and stenting are established modes of treatment in the management of bile duct stones and cholangitis. It is the first choice of treatment when the patients are elderly and have other medical conditions rendering them unfit for surgery. AIMS: We studied the immediate and long-term outcome of endoscopic stenting in patients who underwent Endoscopic Retrograde Cholangiopancreatography (ERCP) for ductal stones. PATIENTS AND METHODS: From January 1990 to December 1992, 366 patients had ERCP done for biliary stone disease. Fifty-five of these patients received a 10 French biliary stent for the treatment of bile duct stones. Most of these patients (60%) had an advanced age of more than 70 years and 45% had co-existing medical conditions rendering them high risk for surgery. Ninety-one percent had stones greater than 1.2 cm in diameter and 82% had multiple stones. RESULTS: Eighty-nine percent of the patients had relief of jaundice and cholangitis resolved in 96% of the patients after stenting. None required immediate surgery after ERCP. Temporary bile drainage was achieved in 19 patients who went for surgery at a later date when they became more stable. Twenty-nine patients were on long-term follow-up for a mean period of 13 months. Nineteen patients still have their stents in place and remained well. The stents were repeatedly changed in 15 patients after an average duration of 5.5 months. Late complications were cholangitis in 2 patients and stent migration in one patient. There was no related mortality. In 5 patients, the stones have either disappeared spontaneously or become smaller and have been removed at subsequent ERCP. CONCLUSION: Endoscopic stenting is a valuable alternative to surgical bile duct exploration. It can be the definitive mode of treatment for large or multiple bile duct stones in the elderly and patients with multiple medical problems who are unfit for surgery.

Adult↗

Endoscopic drainage in benign pancreatic disease: immediate and medium term outcome.

OBJECTIVE: To elucidate further the role of endoscopy in the treatment of benign pancreatic disease. DESIGN: Retrospective study. SETTING: University hospital, Sweden. SUBJECTS: 136 of 319 patients who underwent endoscopic retrograde cholangiopancreatography (ERCP) for known or suspected pancreatic disease had abnormal findings at duodenoscopy or ductography, or both. In 28 patients endoscopic treatment was considered because of recurrent acute pancreatitis (n = 6), chronic pancreatitis (n = 5), pancreatic pain syndrome (n = 5), pancreatic fistula with ascites (n = 1), and pseudocyst (n = 1), or appreciable biliary obstruction from chronic pancreatitis (n = 10). INTERVENTIONS: Pancreatic duct drainage was attempted in 18 patients and successful in 13 (72%). Endoscopic sphincterotomy (EST) alone was done in 7/13 patients and an endoprosthesis (stent) was placed in 6/13. Bile duct drainage was attempted and successful in 10 patients by EST together with dilatation with (n = 6) or without (n = 4) simultaneous placement of a stent. MAIN OUTCOME MEASURES: Relief of pain and cholestasis. RESULTS: Immediate (1-30 days after initial treatment) and medium term (median 48 months after initial treatment) outcome after pancreatic duct drainage was excellent (no pain) or good (occasional mild pain) in 62% (8/13) and 67% (8/12) of the patients, respectively. Complications of the endoscopic procedure were encountered in four patients (31%) and comprised infection with abscess formation (n = 1), repeated stent clogging (n = 1) or stent migration (n = 2). Surgery was subsequently required in three patients (23%) because of intraabdominal abscess (n = 1), recurrent pain (n = 1), or no pain relief (n = 1). At medium term follow-up (median 68 months) after biliary drainage 7/10 patients had liver function tests within the reference ranges. Only two patients required subsequent biliodigestive shunts 7 and 13 months after EST, respectively. CONCLUSION: Our findings favour endoscopic drainage as a safe and effective method for temporary and medium term relief of pain and biliary obstruction in selected patients with benign pancreatic disease.

Adult↗

Characteristics of the chemotactic factor-mediated cell swelling response of tumor cells.

The interaction between Walker carcinosarcoma cells, maintained in ascites form in noninbred Sprague-Dawley rats and in cell culture, and appropriate factors induced directed migration (chemotaxis) of these cells as measured in the Boyden chamber assay. The response of the tumor cells to these factors was very similar to that of leukocytes to their chemotactic factors. In addition to inducing chemotactic responses, the interaction between Walker carcinosarcoma cells and appropriate chemotactic factors also led to a swelling of the cells, which could be measured with the use of Coulter Channelyzer C-1000. Associated with the cell-swelling response was a temporary drop in the cell count of a suspension of cells as measured with the use of a Coulter counter, model ZBI. These responses were also similar to what occurs when leukocytes interact with their chemotactic factors. When the suspension of tumor cells was pretreated with 2-deoxyglucose, the responses measured with the Coulter counters were almost completely abrogated. In contrast to this, treatment of the cells with various chemical agents, including cytochalasin B, colchicine, cycloheximide, chlorpromazine, and the Ca2+ ionophore A23187 failed to significantly reduce the cell-swelling response. Cytochalasin B slightly potentiated the response. These similarities to the responses of leukocytes suggested a common underlying mechanism.

Animals↗

Use of a partially deployed wallstent to act as an inferior vena cava filtration device during coil embolization of a high-flow arteriovenous fistula.

Closure of high-flow arteriovenous (AV) fistulas with endovascular coil embolization can be technically challenging and fraught with complication as a result of the possibility of coil migration into venous circulation and subsequent embolization to distant sites such as the heart and lungs. Filtration of the AV fistula's central venous outflow may allow trapping of coils that inadvertently pass into the venous system during the procedure. This useful measure can reduce the morbidity associated with such interventions. The present report describes an unusual case of a large high-flow renal AV fistula embolized with the use of a partially deployed Wallstent as a temporary filtration device within an enlarged suprarenal inferior vena cava. This method further allows removal of any trapped coils without the need for a snare device.

Aged↗

Population dynamics inferred from temporal variation at microsatellite loci in the selfing snail Bulinus truncatus.

We analyzed short-term forces acting on the genetics of subdivided populations based on a temporal survey of the microsatellite variability in the hermaphrodite freshwater snail Bulinus truncatus. This species inhabits temporary habitats, has a short generation time and exhibits variable rates of selfing. We studied the variability over three sampling dates in 12 Sahelian populations (1161 individuals). Classical genetic parameters (estimators of Ho, He, f, selfing rate and Fst) showed limited change over time whereas important temporal changes of allelic frequencies were detected for 10 of the ponds studied. These variations are not easily explained by selection, sampling drift and genetic drift alone and may be due to periodic migration. Indeed the habitats occupied by the populations studied are subject to large temporal fluctuations owing to annual cycles of drought and flood. In such ponds our results support a demographic model of population expansions and contractions under which available habitats, after the rainy season, are colonized by individuals originating from a smaller number of refuges (areas that never dry out in the deepest parts of the ponds). In contrast, selfing appeared to be an important force affecting the genetic structure in permanent ponds.

Animals↗

Thymic microenvironment: selective activities of soluble and insoluble components.

Two components of the thymic microenvironment have been separated, and their biological properties have been studied. The first component, soluble thymic factor (STF), after injection into syngeneic recipients, provoked a temporary decrease in thymic weight on day 7, concomitant with an increase in the level of T cells in lymph nodes (but not in the spleen), followed by a general hypertrophy of lymph nodes peaking on day 21. In contrast, after the injection of the second component, insoluble thymic fraction (ITF), there was an increase in the number of prothymocytes in the thymic subcapsulary cortex, followed by general thymic hypertrophy on days 14 and 21. STF is believed to trigger a selective migration of lymph-node-seeking T cells from the thymus, whereas ITF seems to play a role in the differentiation step preceding that imparted by STF.

Animals↗

Controlled orthodontic extrusion to create gingival papilla: a case report.

Prosthetic dental restorations alone do not produce esthetics. They must be in harmony with the surrounding gingiva, especially the gingival papilla. This case report describes the enhancement of the gingival papilla between an implant replacing the maxillary left central incisor and a provisional crown on the maxillary left lateral incisor. Esthetic harmony was disrupted by the absence of an interproximal papilla. First, provisional crowns on the implant and adjacent tooth were used in an attempt to remodel the interproximal gingival tissue, but the esthetic result was not satisfactory. The second attempt involved orthodontic extrusion of the lateral incisor in an incisal direction. The gingival tissue migrated incisally, thus restoring the deficient papilla and establishing restorative and gingival harmony. Both the implant and the lateral incisor were restored with ceramic crowns.

Crowns↗

Induction of cytokines, chemokines and adhesion molecule mRNA in a rat forebrain reperfusion model.

Cellular damage secondary to reperfusion following ischemic insult has been hypothetically attributed to an inflammatory cascade concerted by cell-to-cell interactions. While the role of several cytokines and adhesion molecules in reperfusion injury of the brain has been explored to a certain extent, their regulatory and temporary profiles remain unclear. We have addressed the temporal features of the induction of mRNA for proinflammatory cytokines, adhesion molecules and chemokines at an acute phase subsequent to reperfusion in rat forebrain. Semiquantitatively calibrated reverse transcription-polymerase chain reaction analysis was employed to assess the relative expression of mRNA for intercellular adhesion molecule (ICAM)-1, interleukin (IL)-1 alpha, IL-1 beta, 1L-2, 1L-6, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte-chemoattractant protein (MCP)-1, and macrophage migration inhibitory factor (MIF). The increase in mRNA from the basal levels after reperfusion followed one of two different patterns; an increase occurring as early as 1 h, or a slight increase continuing up to 24 h after reperfusion. The former pattern was seen for ICAM-1, IL-1 alpha, IL-1 beta, TNF-alpha, and MCP-1, and the latter for IL-6 and MIF. These results were consistent with the proinflammatory properties of the immediately induced cytokines, which may be involved in the initiation step of the inflammatory cascade, causing the secondary cellular responses and finally leading to further brain damage.

Animals↗

Dietary-induced rapid decrease of microvillar carbohydrase activity in rat jejunoileum.

Activities of several carbohydrases and peptidases were determined in proximal, middle, and distal thirds of the jejunoileum of female 16-wk-old rats that were fed a high-starch (70 cal%), low-fat (7 cal%) diet for 2 wk and also in rats that (after this introductory period) were fed an isocaloric low-starch (5 cal%), high-fat (73 cal%) diet for 1, 2, and 3 days. The body weight changes, food intake, amount of protein per intestinal segment, and rate of enterocyte migration were practically the same in all groups during these experimental periods. The decreased intake of starch was followed by a rapid decrease (40-80%) of carbohydrases (lactase, sucrase, maltase, and glucoamylase) within the first 24 h in total intestinal homogenates--and as studied in cryostat serial sections--in all regions of the jejunal villus-crypt columns, and mainly in proximal and middle segments. In contrast, the activities of leucylnaphthylamidase and L-phenylalanylglycine hydrolase exhibited little change except for a slight temporary decrease of activity on the 1st day in the proximal segment only (25-30%). Thus these data show that a decrease of starch content in an isocaloric diet evokes a rapid decrease in the activity of microvillar carbohydrases and that activity of these enzymes both in mature and immature enterocytes is capable of reacting to a change (decrease) of dietary carbohydrate content.

Animals↗

Temporary resection-arthrodesis of the knee using an intramedullary rod and bone cement.

A temporary arthrodesis of the knee, using an intramedullary Küntscher rod and bone cement, was carried out in 76 patients after resection of tumours of the distal femur or proximal tibia. In contrast to primary arthrodesis with bone grafting, this procedure allows the patient to bear weight on a stable limb a few days after operation, reduces the risk of infection and avoids the negative effect of chemotherapy on graft incorporation. In 12 cases (16%) the implant failed mechanically by fracture, or by bending or migration of the rod, at from 13 to 54 months after operation. Deep infection occurred in 11 patients (14%). A second reconstruction was carried out in 14 patients, 10 using Kotz prostheses and 4 arthrodeses with bone grafting. The operation we describe has advantages for patients with high grade tumours which require chemotherapy and resection-arthrodesis of the knee joint.

Adolescent↗