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Immunohistochemical localization of proteoglycans and non-collagenous matrix proteins in normal and osteochondrotic porcine articular-epiphyseal cartilage complex.

Osteochondrosis is an impaired focal endochondral ossification which appears as a cartilage retention in the subchondral bone of growing pigs. The normal differentiation of chondrocytes does not occur and the matrix calcification is restricted. The present investigation has compared the content of selected macromolecules in the cartilage matrix of the normal articular and epiphyseal growth cartilage with the osteochondrotic cartilage, using a peroxidase-antiperoxidase (PAP) immunocytochemical method at light microscopical level. Some of the non-collagenous macromolecules (fibromodulin, large aggregating proteoglycans, fibronectin, 100-kDa subunit protein and 148-kDa protein) were conspicuously prominent within the osteochondrotic cartilage, compared to the matrix of the "normal" resting, proliferative and hypertrophic regions. This indicates that the chondrocytes in the osteochondrotic cartilage do not modify their surrounding matrix adequately, thus precluding normal calcification.

Animals↗

Preventive health care for pet potbellied pigs.

Many small and exotic animal practitioners decline to see potbellied pigs, possibly because of their challenging behavior and the perception that in order to treat them, one must be very knowledgeable about swine medicine. With some basic information about their unique behavior and their preventive health care needs, however, seeing potbellied pigs in practice is easy and provides a much-needed service in many communities. The behavior of the pig, recommended methods of restraint, physical examination, vaccinations, and parasite control are all discussed.

Animals↗

Potbellied pig husbandry and nutrition.

Vietnamese potbellied pigs, when appropriately cared for, make excellent pets for some people. However, their proper housing, care, and training is more challenging to the average person than that required for most traditional pets, such as dogs and cats. Failure to feed and house the pet pig appropriately results in the most common health and behavior problems. The obese, intractable pet pig gives little pleasure to a pet owner and appears to lead a very poor-quality life, frequently leading to the pet pig being given away or worse. Preventing health and behavior problems is easy if pet owners are armed with correct information early, either before or immediately after their acquisition of a pet pig. A veterinarian prepared to share this information can save the lives of many pigs as well as keep clients happy and coming back.

Animal Husbandry↗

Emergency care for potbellied pigs.

Because of the limited number of veterinarians treating potbellied pigs, many pet pigs do not receive proper preventative health care. The potbellied pig's continued popularity among urban dwellers ensures that veterinarians who treat small and exotic animals will be contacted by owners of pet pigs with real or perceived emergencies. Regardless of their knowledge of swine, the information contained in this article should enable any veterinarian to provide basic emergency care for pet pigs.

Animal Husbandry↗

Observations on the suitability and importance of the domestic intermediate hosts of Echinococcus granulosus in Uttah Pradesh, India.

The present study investigated the suitability and importance of buffaloes, camels, sheep, goats and pigs in maintaining the life-cycle of Echinococcus granulosus in Aligarh, India. A total of 565 (36%) of 1556 buffaloes, 20 (2%) of 1208 goats, 5 (1%) of 559 pigs, 6 (6%) of 109 sheep and two of three camels were found to harbour hydatid cysts. The frequency distribution of the hydatid cysts in each intermediate host species was over-dispersed and in buffaloes cyst fertility increased with increasing cyst size. Of 2171, 95 and four buffalo, goat, and camel cysts examined 327 (15%), two (2%) and three cysts respectively were fertile. No pig or sheep cysts were found to contain protoscoleces. The unfenced buffalo abattoir and the large number of dogs allowed access to the abattoir coupled to the number of buffalo slaughtered in comparison to the other potential hosts, indicates that the buffalo is the most significant host for maintaining the life-cycle of the parasite in this area of India. Applicable control measures for the region are suggested.

Abattoirs↗

Glucose 6-phosphate dehydrogenase: isoenzymatic pattern in Oesophagostomum venulosum, Trichuris ovis and T. suis.

In the present communication we have studied the isoenzymatic pattern activity of the glucose 6-phosphate dehydrogenase (G6PD) in Oesophagostomum venulosum, Trichuris ovis and T. suis, parasites of Capra hircus (goat), Ovis aries (sheep) and Sus scrofa domestica (pig) respectively, by polyacrylamide gel electrophoresis. Different phenotypes have been observed in the G6PD isoenzymatic pattern activity in males and females of Oesophagostomum venulosum. Furthermore, G6PD activity has been assayed in Trichuris ovis collected from Ovis aries and Capra hircus. No differences have been observed in the isoenzymatic patterns attending to the different hosts. All the individuals exhibited one single band or two bands; this suggests a monomeric condition for G6PD in T. ovis. In T. suis the enzyme G6PD appeared as a single electrophoretic band in about 85.7% of the individuals.

Alleles↗

Trends in salmonella food poisoning in England and Wales 1941-72.

Cattle and pig herds and flocks of domestic fowl have formed the main reservoir of human salmonella food poisoning in England and Wales from 1941 to 1972. Changes in the incidence of human salmonella food poisoning and in the serotypes of salmonellas isolated from human infections are shown to have been associated with the introduction of new foods, with changes in animal husbandry, and with changes in the relative proportions of flesh food from different species consumed. New foods, dried powdered egg, liquid egg and frozen liquid egg were introduced during the period of food rationing which extended from 1940 to 1953. Changes in animal husbandry, in particular the intensive production of pigs, poultry and eggs, followed the re-establishment of pig herds and fowl flocks after the derationing of animal feed in 1953. The changes in the proportions of flesh foods consumed followed the introduction of frozen oven-ready fowl in the late 1950s and early 1960s which by 1964 became cheaper than traditional flesh foods.

Animals↗

Studies on the 1967--68 foot and mouth disease epidemic: incubation period and herd serial interval.

The incubation period during this epidemic was studied using both a spectral analysis-cum-filtering method and analysis of case histories. Using spectral analysis, the modal herd serial interval was estimated to be 8--10 days based on the record of the daily number of outbreaks and an adjusted cattler series. The case histories tended to confirm these estimates but indicated that the serial interval varied considerably between species. The filtering method revealed that the herd serial interval apparently changed during the epidemic. For the first 4 weeks the interval was 8 days, while in the latter stages it was about 2 weeks.

Animals↗

Characterization of Yersinia enterocolitica strains isolated from cattle, sheep and pigs in the United Kingdom.

The properties of 48 cultures identified as Yersinia enterocolitica or Y. enterocolitica-like organisms, including Y. frederiksenii, Y. intermedia and Y. kristensenii, were examined. Of these, 39 were isolated from faeces of apparently healthy pigs, five from healthy cattle, one from an aborted bovine fetus, one from an aborted lamb and one from a lamb suffering from acute enteritis. Most isolates from healthy animals were of Y. enterocolitica biotype 1 or Y. intermedia and belonged to O serogroups not usually associated with disease in man or animals. The isolates from abortion or enteritis cases were of Y. enterocolitica biotypes 3, 4 and 5 and belonged to the pathogenic serogroups 0:5b and 0:2a, 2b, 3. No organisms of serogroup 0:9 were found.

Agglutination↗

Salmonella isolations in abattoirs in Greece.

The prevalence of salmonellas in drain swabs from three abattoirs of Athens was studied with the use of conventional methods of Salmonella isolation as well as with a new procedure which involves secondary enrichment from the usual selenite broth or Muller-Kauffmann's tetrathionate broth in Rappaport's medium slightly modified.In all groups studied the secondary enrichment in Rappaport's medium led to an increase in the number of positive swabs, in the number of Salmonella serotypes, and in the total number of strains isolated.The frequency of Salmonella isolations was higher in samples from abattoirs killing only pigs and lower in samples from abattoirs killing only cattle or only sheep.The predominant serotype in abattoirs dealing with cattle was Salmonella tennessee, and S. typhimurium in abattoirs dealing with sheep. No predominant serotype was found in samples from abattoirs dealing mostly with pigs.S. abony, S. drypool, S. emek, S. indiana, S. muenchen and S. tennessee were isolated for the first time in Greece.

Abattoirs↗

The Hampshire epidemic of foot-and-mouth disease, 1967.

An analysis was made of the spread of foot-and-mouth disease during the epidemic in Hampshire in January and February 1967. To explain the pattern of spread, it had to be postulated that virus was present seven days before the first outbreak was reported. It is suggested that the disease occurred initially in pigs fed on infected meat and that the virus was subsequently disseminated from the local abattoir, where the pigs were killed, to four farms by movement of animals, slaughterhouse waste, people or vehicles, and to fifteen by the airborne route. Subsequent spread from these farms was by movement in two instances and by the airborne route in five. The source and route of infection of the last farm in the outbreak were not determined.The risk of spread through movement was associated more with carriage of infected slaughterhouse waste, movement of animals, people or vehicles carrying animals than through collection of milk, artificial insemination or movement of other types of vehicles. Outbreaks of disease among pigs gave rise to more secondary spread than outbreaks in cattle. Secondary outbreaks attributed to airborne spread occurred only in ruminants. Most airborne spread was into areas of high livestock density and cattle in the larger herds became infected. Airborne spread could be correlated with wind direction and speed but not with rain. The reduction in the number of outbreaks at the end of the epidemic could be attributed to the elimination of the largest sources of virus, the control of movements and the fact that in all instances except two the wind was blowing virus over towns and out to sea, to areas of low stock density and to areas where animals had been killed.

Abattoirs↗

Incidence of salmonella infection in animals in England and Wales 1968--1974.

Of a total of 23,609 incidents of salmonella infection reported during the period 1968--74, 20,326 occurred in cattle, 1744 in poultry and other birds, 675 in sheep, 558 in pigs and 306 in other species of animals. Despite the large number of serotypes isolated (153), 88% of incidents were due to only two stereotypes: S. dublin (15,929 incidents of which 15,446 occurred in cattle ) and S. typhimurium (4842 incidents of which 3785 occurred in cattle and 732 in birds). S. choleraesuis was the third (314 incidents which with only 5 exceptions occurred in pigs) and S. abortusovis (243 incidents all in sheep) was the fourth most commonly isolated serotype. The following six serotypes were each recorded in more than 100 incidents: S. newport (177), S. agona (170), S. virchow (169), S. anatum (152), S. enteritidis (150) and S. montevideo (111). The other 143 serotypes accounted for only 5-8% of total incidents. The trends of annual incidence of salmonella infection in cattle, sheep, pigs, poultry and other birds during the 17-year period 1958-74 and the distribution of the main serotypes in the individual species of animals from 1968 to 1974 are discussed.

Animals↗

A survey of Campylobacter in animals.

A survey of Campylobacter species in the faeces or rectal contents of domestic animals was carried out using direct and enrichment culture methods. Campylobacters were isolated from 259 (31%) of 846 faecal specimens. The highest isolation rate was found in pigs (66%); lower rates were found in cattle (24%) and sheep (22%). In pigs all the isolates were C. coli, in sheep and cattle about 75% were C. jejuni. Only five isolations of C. fetus subsp. fetus were made, all from cattle. More pigs with diarrhoea had C. coli in their faeces than healthy pigs (77% vs 47%), but such a clear difference in isolation rate between sick and healthy animals was not seen in cattle or sheep. The enrichment method increased the total isolation rate of C. jejuni and C. coli by 33%, but for cattle specimens it increased it by 69% (from 6.5% to 21%). However, the enrichment method failed to detect 16% of positive specimens (mainly C. coli), so direct and enrichment methods should be used for the culture of campylobacters from animal faeces. The results show that cattle, sheep and pigs constitute a large potential source of campylobacter infection for man.

Animals↗

Transmission of taeniid tapeworm eggs via blowflies to intermediate hosts.

Experiments showed that dead blowflies containing eggs of Taenia hydatigena can transmit infection if ingested by lambs during grazing. Rabbits became infected with T. pisiformis after grazing grass exposed to blowflies that had been in contact with dog faeces containing the eggs of this parasite. When captive blowflies were exposed first to dog faeces containing proglottids of T. hydatigena and then to cooked meat, 100% of pigs fed on this meat became infected. The experiments are intended to model the transmission dynamics that result in human larval tapeworm infections. The implications of the results to the epidemiology of human hydatid diseases caused by Echinococcus granulosus and E. multilocularis as well as of T. solium cysticercosis are discussed.

Animals↗