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Eugenics in Japan: some ironies of modernity, 1883-1945.

Japanese eugenic discourse and institution building contrast sharply with comparable movements elsewhere. As a social-intellectual phenomenon, Anglo-American eugenics considered the Japanese racially inferior to Western peoples; yet eugenic ideals and policies achieved a remarkable popularity in Japan. Most of mainstream Japanese genetics was derived from orthodox Mendelian roots in Germany and (to a lesser degree) the United States. But French-style Lamarckian notions of the inheritability of acquired characters held surprising popularity among enthusiasts of eugenics. Japanese eugenicists could condemn the actions of foreign eugenicists like Charles Davenport in the United States for their efforts to forbid Japanese immigration in the 1920s, yet appeal to these same eugenicists as a source of legitimacy in Japan. These paradoxes can partly be explained against a background of relative isolation in a period of profound social change. Few Japanese eugenicists had close personal contact with foreign eugenicists, and most of their knowledge was acquired through reading rather than direct exposure. The eugenic ideal of ethnic purity was attractive to a society long accustomed to monoracial self-imagery. The need to defend national independence in an era of high imperialism seemed to require the most up-to-date policies and ideas. And Japan's own acquisition of an overseas empire seemed to demand a population management philosophy ostensibly based on scientific principles. These and other forces supported the implementation of eugenic policies and prescriptions among the Japanese people in the first half of the twentieth century.

Asian People↗

vanA gene cluster in a vancomycin-resistant clinical isolate of Bacillus circulans.

We report on the cloning and sequencing of the vanA gene cluster present in the glycopeptide-resistant clinical isolate Bacillus circulans VR0709 (R. Fontana, M. Ligozzi, C. Pedrotti, E. M. Padovani, and G. Cornaglia, Eur. J. Clin. Microbiol. Infect. Dis. 16:473-474, 1997). The presence of a vanA-related gene in VR0709 was demonstrated in a PCR assay which permitted the specific amplification of an internal segment of vanA. Southern blotting suggested that the vanA gene was located in the chromosome in a 7. 6-kb EcoRI fragment. DNA sequence analysis revealed the presence of all seven genes of the vanA cluster (vanR, vanS, vanH, vanA, vanX, vanY, and vanZ). The degree of identity between homologous proteins encoded by Tn1546 and the chromosome of B. circulans VR0709 ranged from 87 to 95%. Neither PCR nor Southern blotting with specific primers and probes, respectively, showed the presence of open reading frames (ORFs) 1 and 2 which encode the transposase and the resolvase of Tn1546, respectively, the transposon found to carry the vanA gene cluster in enterococci. Determination of the sequences of the flanking regions of the van gene cluster of B. circulans revealed perfect inverted repeats of 10 bp which delineated a 9.2-kb region containing the van gene cluster and an ORF which encoded a putative protein (178 residues) which displayed a low level of identity (28%) to the resolvase of Tn1546. These results suggest that glycopeptide resistance in B. circulans VR0709 is associated with the acquisition of a vanA gene cluster which shows a high degree of homology with that of enterococci. In B. circulans, however, the cluster is not carried by Tn1546 and is borne by the chromosome.

Amino Acid Sequence↗

Computerized planimetry versus clinical assessment for the measurement of cervical ectopia.

OBJECTIVE: As part of a study to assess the role of cervical ectopia in the acquisition of cervical infections, we determined the reliability of cervical ectopia measurements made by computer planimetry and by clinical (visual) assessment. STUDY DESIGN: We conducted pelvic examinations of 1004 women seeking contraceptive services at two health centers in Baltimore. After application of acetic acid, clinicians estimated the relative area of ectopia by visual inspection and took cervical photographs. Two independent raters measured the absolute and relative areas of ectopia from the digitized images by means of an analytic software program. Agreement levels between raters, between multiple readings by the same rater, and between the two measurement methods were quantified by means of the intraclass correlation coefficient and weighted kappa. RESULTS: Intrarater agreement was excellent for computer planimetry measurements of the absolute (intraclass correlation coefficient, 0.97) and relative (intraclass correlation coefficient, 0.89) areas of ectopia. Interrater agreement was also high for computer planimetry measurements of the absolute (intraclass correlation coefficient, 0.83) and relative (intraclass correlation coefficient, 0.85) areas of ectopia. Agreement levels were moderate between clinician assessment and computer planimetry measurements of the relative area of ectopia (kappa = 0.48), but agreement was better when clinical assessment was limited to observations by a single, experienced clinician. CONCLUSION: Measurement of cervical ectopia by computer planimetry was highly reliable and appears appropriate for assessment of the role of ectopia in the acquisition of cervical infections. Clinical assessment of cervical ectopia may be used when computer planimetry is not available.

Cervix Uteri↗

Beilong virus, a novel paramyxovirus with the largest genome of non-segmented negative-stranded RNA viruses.

During a subtraction study on gene expression in human kidney mesangial cells (HMCs), cDNA clones with sequence homology to paramyxovirus P, M and F genes were isolated. Subsequent investigation revealed that this particular HMC line was infected with a previously unknown paramyxovirus. Here, we report the isolation and genome characterization of this new virus, now named Beilong virus (BeV). The genome of BeV is 19,212 nucleotides (nt) in length and is the largest among all known members of the order Mononegavirales. The BeV genome contains eight genes in the order 3'-N-P/V/C-M-F-SH-TM-G-L-5'. The SH and TM genes code for a small hydrophobic protein of 76 aa and a transmembrane protein of 254 aa, respectively. The BeV G gene, at 4527 nt, codes for an attachment protein of 734 aa and contains two additional open reading frames (ORFs) in the 3' half of the gene, coding for putative proteins of 299 and 394 aa in length. Although the exact origin of BeV is presently unknown, we provide evidence indicating that BeV was present in a rat mesangial cell line used in the same laboratory prior to the acquisition of the HMC line, suggesting a potential rodent origin for BeV.

Amino Acid Sequence↗

New findings on evolution of metal homeostasis genes: evidence from comparative genome analysis of bacteria and archaea.

In order to examine the natural history of metal homeostasis genes in prokaryotes, open reading frames with homology to characterized P(IB)-type ATPases from the genomes of 188 bacteria and 22 archaea were investigated. Major findings were as follows. First, a high diversity in N-terminal metal binding motifs was observed. These motifs were distributed throughout bacterial and archaeal lineages, suggesting multiple loss and acquisition events. Second, the CopA locus separated into two distinct phylogenetic clusters, CopA1, which contained ATPases with documented Cu(I) influx activity, and CopA2, which contained both efflux and influx transporters and spanned the entire diversity of the bacterial domain, suggesting that CopA2 is the ancestral locus. Finally, phylogentic incongruences between 16S rRNA and P(IB)-type ATPase gene trees identified at least 14 instances of lateral gene transfer (LGT) that had occurred among diverse microbes. Results from bootstrapped supported nodes indicated that (i) a majority of the transfers occurred among proteobacteria, most likely due to the phylogenetic relatedness of these organisms, and (ii) gram-positive bacteria with low moles percent G+C were often involved in instances of LGT. These results, together with our earlier work on the occurrence of LGT in subsurface bacteria (J. M. Coombs and T. Barkay, Appl. Environ. Microbiol. 70:1698-1707, 2004), indicate that LGT has had a minor role in the evolution of P(IB)-type ATPases, unlike other genes that specify survival in metal-stressed environments. This study demonstrates how examination of a specific locus across microbial genomes can contribute to the understanding of phenotypes that are critical to the interactions of microbes with their environment.

Adenosine Triphosphatases↗

Complete mitochondrial genomes of the three brown algae (Heterokonta: Phaeophyceae) Dictyota dichotoma, Fucus vesiculosus and Desmarestia viridis.

We report the complete mitochondrial sequences of three brown algae (Dictyota dichotoma, Fucus vesiculosus and Desmarestia viridis) belonging to three phaeophycean lineages. They have circular mapping organization and contain almost the same set of mitochondrial genes, despite their size differences (31,617, 36,392 and 39,049 bp, respectively). These include the genes for three rRNAs (23S, 16S and 5S), 25-26 tRNAs, 35 known mitochondrial proteins and 3-4 ORFs. This gene set complements two previously studied brown algal mtDNAs, Pylaiella littoralis and Laminaria digitata. Exceptions to the very similar overall organization include the displacement of orfs, tRNA genes and four protein-coding genes found at different locations in the D. dichotoma mitochondrial genome. We present a phylogenetic analysis based on ten concatenated genes (7,479 nucleotides) and 29 taxa. Stramenopiles were always monophyletic with heterotrophic species at the base. Results support both multiple primary and multiple secondary acquisitions of plastids.

Base Sequence↗

Objectivating nasality in healthy and velopharyngeal insufficient children with the Nasalance Acquisition System (NasalView). Defining minimal required speech tasks assessing normative values for Dutch language.

OBJECTIVES: (1) To define normative nasalance data for Dutch language with "the NasalView System", and obtain a reference for normality when nasality is evaluated in children. (2) To investigate the minimal number of required speech tasks for a reliable nasalance measurement. METHODS: 55 children (30 normal and 25 velopharyngeal insufficient), aged between 4 and 11 were included. All children had to read or repeat two Dutch passages ((one with a normal amount of nasal consonants (normal passage) and one with none (nonnasal passage)). Further, one normal and one velopharyngeal insufficient subject read a passage in repetition to test the NasalViews reproducibility: (1) For both passages, group means (GM) and standard deviations (S.D.) were used to compute "pathological nasalance boundaries" [GM +/- (2 x S.D.)], in combination with the coefficient of variation (CV), sensitivity, specificity and positive predictive values. (2) With ANOVA all sentences within each passage were tested for significant differences in nasalance. RESULTS: (1) The pathological boundaries were 28.6-41.4% (GM: 35.0) and 21.4-34.7% (GM: 28.1), for the normal and nonnasal passage, respectively. For the normal passage a sensitivity of 96%, a specificity of 93% and a positive predictive value of 92% was computed. For the nonnasal passage these parameters were 96, 95 and 96%, respectively. Intra subject CVs of 3.6% (normal subject) and 1.5% (VI subject) showed good reproducibility of measurements. (2) Within the normal passage only the third sentence was significantly different in nasalance, compared to the entire passage (31.2% versus 35.0%). Within the nonnasal passage the second and fifth sentences were significantly different (23.8 and 24.8% versus 28.1%). However, the individual nonsignificantly different sentences showed a higher variation in nasalance compared to the entire passages. CONCLUSIONS: The NasalView System seems to be reliable and quantifies valid nasalance values when nasality is evaluated. Within both passages high levels of sensitivity, specificity and positive predictive values were obtained. The nonnasal passage discriminates slightly better for hypernasal speech. For the most reliable nasalance measurements, the entire passage should be used.

Analysis of Variance↗

Acquisition of word meanings from context by deaf readers.

In this paper we examine issues concerning the acquisition of word meanings from context by deaf readers. Theories and research with hearing readers suggest several reader, text, and task factors which may influence this acquisition process, although a similar research base with deaf readers is lacking. It is our view that a variety of important questions remain unanswered concerning deaf readers' meaning acquisitions. This paper reviews relevant findings from recent investigations of how individuals use context in deriving word meanings. It details several exemplary studies and draws implications for future inquiry.

Deafness↗

Improved acid tolerance of a recombinant strain of Escherichia coli expressing genes from the acidophilic bacterium Oenococcus oeni.

AIMS: Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RESULTS: Sequence analysis showed that orfB encodes a putative transcriptional regulator of the LysR family. The protein encoded by orfC shares homologies with multi-drug resistance systems. Heterologous expression of orfB, orfC and hsp18 genes in Escherichia coli significantly enhanced the viability of the host strain under acidic conditions. CONCLUSION: It was demonstrated that the three genes were needed for acquisition of this acid tolerance phenotype. SIGNIFICANCE AND IMPACT OF THE STUDY: Heterologous expression of Oenococcus genes could be used to confer acidophilic behaviour on strains of biotechnological interest.

Acids↗

Change of voice characteristics during +3 Gz acceleration.

BACKGROUND: This study was performed to test the feasibility of an experimental approach for assessing voice changes during exposure to increased +Gz acceleration. Such changes are probably due to mechanical alterations of the structures involved in voice production. This may be relevant to automatic speech analysis for flight control. Because voice control by means of auditory feedback may compensate for acceleration effects, the investigations included conditions with masked auditory feedback. METHOD: Four male subjects read standard speech test material while seated in a human centrifuge both during sustained +3 Gz acceleration and in a reference condition. Both test runs were repeated with masking of the auditory feedback by a white noise presented via headphones. Microphone and acceleration signals were recorded on a PC-based dynamic signal acquisition board. Long-time average spectra (LTAS), fundamental frequency (F0), and the frequency of the first, second, and third formant (F1, F2, F3) of the vowels /a/, /o/, and /i/ were extracted from the microphone signal for the different conditions. RESULTS: LTAS clearly differed between the masking conditions, but not between reference and +3 Gz conditions. F0 clearly rose with auditory masking and showed a small increase under +3 Gz acceleration. Several effects of +3 Gz acceleration on formant frequencies were found, all of rather small magnitude. Increased acceleration lowered F2 for the vowel /i/. A decrease of F3 was observed for vowels /i/ and /o/. CONCLUSION: This pilot study has shown the feasibility of an experimental approach to assessing voice changes during exposure to increased +Gz acceleration. Exposure to +3 Gz showed small effects on FO and several formant frequencies. A definitive forthcoming study should assess the significance of these effects by investigating a greater number of subjects during exposure to acceleration higher than +3 Gz.

Adult↗

[Simultaneous assessment of perfusion and myocardial viability with 2 isotope studies (thallium at rest and sestamibi in exercise). Initial experience in Mexico and Latin America].

UNLABELLED: Rest-stress sestamibi single photon emission computed tomography (SPECT) has sensitivity and specificity similar to those of thallium 201 SPECT for detection of coronary artery disease. However, sestamibi is not ideal agent to study myocardial viability. There is not published experience in Latin American using dual isotope SPECT protocol to evaluate myocardial perfusion and viability. We studied 44 consecutive patients with coronary artery disease, 37 of them with previous myocardial infarction. Coronary angiography was performed in all patients. We used a 3 mCi rest T 201 SPECT followed by stress and 25 mCi sestamibi injection. Sestamibi SPECT was performed 30 minutes after exercise or 1 hour after pharmacologic stress with dipyridamole. To validate perfusion findings patients returning next day for rest sestamibi injection and SPECT. Scintigraphic data were read by two blinded expert using 20 SPECT segment analysis and each segment was scored using 5 points scoring system (0 = normal, 4 = absent uptake). The segmental score agreement between rest thallium 201 and rest sestamibi and the comparison of defect reversibility percentage and non reversibility between both protocols was 90.7%. CONCLUSION: Separate acquisition dual isotope myocardial perfusion SPECT is accurate for coronary artery disease evaluation. It showed a good agreement with rest-stress sestamibi SPECT for assessment of rest perfusion defects and reversibility and it was a better method to evaluate myocardial viability.

Exercise Test↗

Sign language acquisition by a global aphasic.

A left hemisphere-damaged, hemiplegic, and globally aphasic man was taught receptive and expressive language using Total Communication--a combination of standardized sign language and speech. After an initial period during which words (in sign) were learned slowly and with frequent lapses of memory, the subject's learning ability and memory improved dramatically. He has learned to communicate using increasingly complex, syntactically correct sentences, self-correcting his mistakes. He reads and understands simple sentences. These results suggest that it may be possible to restore communicative function even in those stroke victims who remain orally aphasic if a suitable means of expression can be found.

Aphasia↗

Role of transcriptional repressors in transformation by bovine papillomavirus type 1.

Transformation of rodent cells by bovine papillomavirus type 1 (BPV-1) has been shown to require the direct contribution of the viral oncogenes encoded by the E5, E6, and E7 translational open reading frames (ORFs). It is also known that the viral E1 and E2 ORFs contribute indirectly to cellular transformation through their transcriptional modulation of these viral oncogenes. A mutant BPV-1 disrupted in two of the proteins encoded by the E2 ORF, the E2 transcriptional repressors, has a complex transformation phenotype. In this paper, we provide evidence that (i) this phenotype is not attributable to the absence of other viral genes that overlap the E2 repressor genes, (ii) the acquisition of transformation over time in cells harboring the E2 double-repressor mutant correlates with reversions of the mutations that disrupt one of the E2 repressor genes, E8/E2TR, and (iii) the initial transformation defect in the E2 double-repressor mutant can be rescued by disruption of the full-length protein encoded by the E1 ORF, the E1 transcriptional repressor. We propose a model by which the combination of E1 and E2 transcriptional repressors functions to modulate the transforming capacity of the virus.

Animals↗

Mucin biosynthesis: bovine C2GnT-M gene, tissue-specific expression, and herpes virus-4 homologue.

Mucin glycans are the major determinant of mucin functions. Mucin glycan branch structures, which increase structural heterogeneity and thus functional potential, are extended from beta6 N-acetylglucosaminides formed by beta6 N-acetylglucosaminyltransferases (beta6GnT). Core 2 beta6GnT-M (C2GnT-M) is the only branching enzyme that can synthesize all known mucin beta6 N-acetylglucosaminides. We report the cloning of four different bovine (b) C2GnT-M transcripts that are different only at 5'-untranslated regions. Two bC2GnT-M transcripts are found exclusively in tracheal epithelium and testis, whereas the other two are found in all other mucus-secreting tissues. The bC2GnT-M gene contains four exons spanning 5.3 kb, and the entire open reading frame is in one exon. The bC2GnT-M ORF has 95, 83, and 75% sequence identity to those of bovine herpes virus type 4 (BHV-4), human, and rat C2GnT-Ms, respectively. The homology between bovine and BHV-4 C2GnT-M genes is in the region between 170 nucleotides upstream from ATG start codon and 114 nucleotides downstream from TGA stop codon of the viral gene. Localized at the nonconserved region of the viral genome, the BHV-4 C2GnT-M gene is the only known viral C2GnT-M gene. The results suggest that BHV-4 acquired its C2GnT-M gene from the bovine gene. The mechanism of the viral acquisition of bC2GnT-M gene and the roles of the C2GnT-M gene in the survival and pathogenesis of this virus remain to be elucidated.

5' Untranslated Regions↗

Acquisition of lipreading in a deaf multihandicapped child.

Operant techniques were used to develop lipreading in a nine-year-old deaf boy who had failed to respond appropriately to previous speechreading training. Remediation was initiated with two experiments: the first entailed a reversal and the second a multiple baseline. A component analysis revealed that both tokens and social reinforcers presented simultaneously or separately were effective in shaping lipreading. In addition, the child learned to lip-read either voiced or nonvoiced names of objects.

Affective Symptoms↗

Phage conversion of exfoliative toxin A production in Staphylococcus aureus.

The staphylococcal exfoliative toxins (ETs) are extracellular proteins that cause splitting of human skin at the epidermal layer during infection in infants. Two antigenically distinct toxins possessing identical activity have been isolated from Staphylococcus aureus, ETA and ETB. The gene for ETA (eta) is located on the chromosome, whereas that for ETB is located on a large plasmid. The observation that relatively few clinical isolates produce ETA suggests that the eta gene is acquired by horizontal gene transfer. In this study, we isolated a temperate phage (phiETA) that encodes ETA and determined the complete nucleotide sequence of the phiETA genome. phiETA has a head with a hexagonal outline and a non-contractile and flexible tail. The genome of phiETA is a circularly permuted linear double-stranded DNA, and the genome size is 43 081 bp. Sixty-six open reading frames (ORFs) were identified on the phiETA genome, including eta, which was found to be located very close to a putative attachment site (attP). phiETA converted ETA non-producing strains into ETA producers. Southern blot analysis of chromosomal DNA from clinical isolates suggested that phiETA or related phages are responsible for the acquisition of eta genes in S. aureus.

Animals↗

Social class and preschool language skill. I. Introduction.

This series of studies concerns the relative validity of two explanations of why children of parents of poverty do poorly in school. Both explanations assume that nearly all children have the genetic potential to learn what the schools attempt to teach. One holds that children, and especially the black children, of poverty are in no way deficient in cognitive and linguistic skills. They fail merely because they enter school with a dialect differing from standard English. This complicates the task of learning to read in standard English. The other view holds that the rearing conditions in families of poverty limit the number and variety of objects and places and action models with which the poor child becomes acquainted during his infancy and preschool years, resulting in a cognitive deficit. These conditions also hamper the poor child's acquisition of phonemic symbols for objects and places and the relationships among them, resulting in a deficit in semantic mastery. This series of studies employs several strategies of investigation. This paper indicates their nature. It also describes in some detail the economic and educational characteristics of the parents of the two sets of children examined.

Black or African American↗

A novel transforming gene, hst, from human stomach cancers and a non-cancerous portion of stomach mucosa.

DNAs from 21 human stomach cancers, 16 metastatic stomach cancers to lymph nodes, and 21 non-cancerous mucosae of the stomach from a total number of 26 patients with stomach cancer were assayed for their transforming activity to NIH3T3 cells. Three samples of DNA were positive in transfection assay; one was from a primary stomach cancer of one patient (No. 361), the second was from a non-cancerous portion of stomach mucosa of the same patient (No. 363) and the third was from a lymph node metastasis of stomach cancer from another patient (No. 51). A portion of the transforming gene was cloned and a cDNA clone for this gene was isolated. The reading frame essential for the transforming activity was identified. From the results of sequence analysis of cDNA and Southern blot analysis, this transforming gene did not have homology with any oncogene reported previously. We applied the term, hst (human stomach cancer), to this novel transforming gene. This hst gene was not only responsible for acquisition of transforming activity of No. 361 DNA, but also for that of No. 363 and No. 51 DNAs; all the transformants induced by Nos. 361, 363, and 51 DNAs contained hst gene.

Cell Transformation, Neoplastic↗