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Rates of ethylene release, photosynthesis and transpiration of rice measured in closed-type chamber.

To obtain the basic data of gas exchange of rice (Oryza sativa L. cv. Nipponbare), rates of ethylene release, photosynthesis and transpiration of the rice plant were measured by using a closed-type chamber. Each rate increased until the heading stage and thereafter decreased. Ethylene release rate (E) gradually increased with day after seeding and rates of photosynthesis (P) and transpiration (T) did exponentially. At the heading stage, E, P and T were maximum and had values of about 2.0 mmol plant-1 h-1, 3.0 mmol plant-1 h-1 and 0.60 mol plant-1 h-1, respectively. E in the light period was 1.5-3 times as much as that in the dark period, whereas T in the light period was 5-6 times as much as that in the dark period. E of rice per plant was lower than those of lettuce and Brassica genera which were reported previously. Especially, the rate of rice was about one-tenth that of lettuce. However, when ethylene release rates were estimated on a growth area basis, the rate of rice was about half that of lettuce, and was more than those of Brassica genera.

Ecological Systems, Closed↗

Development of photosynthesis and respiration measurement system using mass spectrometry.

A closed gas-exchange system was developed to measure gross photosynthesis and respiration discriminately and simultaneously. The system developed in this study included a high performance mass spectral analyzer for gas measurements. The gas-exchange system consisted of a 3L assimilation leaf chamber, a 1L flexible metallic bag, gas supply apparatus, and a lighting system. The lights were turned on and gas measurements were started after the initial 12CO2 concentration level was increased to 500 ppm. The 13CO2 gas was added to the chamber 10 min after the start of the light period. The lights were turned off 15 min after the addition of 13CO2. The 12CO2 and 13CO2 concentrations in the chamber during the light and dark periods were measured for pothos and maize leaves. The 13CO2 absorption rate by the leaves was larger than that for 12CO2 during the light period. It was assumed that the 13CO2 absorbed by photosynthesis was not evoluted by respiration during the first 25 min from the start of the light period. Based on this assumption, gross photosynthetic rate and respiration rate were estimated by calculating the difference in uptake rates of 12CO2 and 13CO2 during the light period.

Argon↗

Discriminating and continuous measurement of photosynthesis and respiration by monitoring 13CO2 and 12CO2 as tracers.

The simultaneous and discriminative measurement of the photosynthesis and the respiration of the plant was attained by simultaneous monitoring of 13CO2 and 12CO2 by artificial control of 13CO2 abundance of ambient air. The principle of the measurement is based on the following physiological processes. 6CO2 + 12H2O --> C6H12O6 + 6O2 + 6H2O, 6(13C)O2 + 12H2O --> (13C6)H12O6 + 6O2 + 6H2O, 6CO2 + 12H2(18O) --> C6H12O6 + 6(18O)18O + 6H20. Assuming that respiratory consumption of the new born carbon substrate fixed by photosynthesis is negligible during the measurement, the photosynthetic CO2 consumption VPCO2 and the respiratory CO2 production VRCO2 are measured according to the estimation (1) or (2), (1) for closed method, VPCO2 = k(V0 - V t)¿ F13CO2 + (F12CO2/F13CO2)F13CO2 ¿, VRCO2 = k(V0 - V t)¿ F12CO2 - (F12CO2/F13CO2)F13CO2 ¿, (2) for open method, VPCO2 = kVE ¿ (FI13CO2 - FE13CO2) + (F12CO2/F13CO2)(FI13CO2 - FE13CO2) ¿, VRCO2 = kVE ¿ (FI12CO2 - FE12CO2) - (F12CO2/F13CO2)(FI13CO2 - FE13CO2) ¿ where V0 is initial volume of growth chamber including attached flexible bag, FICO2 is the inlet or initial gas concentration of CO2 and FECO2 is the ambient gas concentration of CO2 in the chamber, V and VE are the sampling rate of mass spectrometer and the ventilation rate of the growth chamber respectively, k is the STPD conversion factor = ¿273(PB-PH2O)/760(273+tE)¿, tE(degrees C) is the ambient gas temperature. In the closed method, the gas container of the growth chamber is circulated, resulting FECO2 is varied according to the balance of consumption and production of CO2, while in the open method VE is controlled to keep FECO2 at a constant value. Both (1) and (2) methods were examined and evaluated on the measurements of komatsuna and maize.

Argon↗

Growth and photosynthesis of Japanese flowering cherry under simulated microgravity conditions.

The photosynthetic rate, the leaf characteristics related to photosynthesis, such as the chlorophyll content, chlorophyll a/b ratio and density of the stomata, the leaf area and the dry weight in seedlings of Japanese flowering cherry grown under normal gravity and simulated microgravity conditions were examined. No significant differences were found in the photosynthetic rates between the two conditions. Moreover, leaf characteristics such as the chlorophyll content, chlorophyll a/b ratio and density of the stomata in the seedlings grown under the simulated microgravity condition were not affected. However, the photosynthetic product of the whole seedling under the simulated microgravity condition increased compared with the control due to its leaf area increase. The results suggest that dynamic gravitational stimulus controls the partitioning of the products of photosynthesis.

Chlorophyll↗

Evolution. When did photosynthesis emerge on Earth?

Life began early in Earth's history, but it was the emergence of photosynthesis that allowed its proliferation across the planet, because it freed life from its sole dependence on abiotic chemical sources of reducing power. Des Marais discusses recent geological and molecular biological evidence, such as the paper by Xiong et al., that photosynthesis emerged at least 2,800 million years ago.

Bacteria↗

Growth in elevated CO(2) can both increase and decrease photochemistry and photoinhibition of photosynthesis in a predictable manner. Dactylis glomerata grown in two levels of nitrogen nutrition.

Biochemically based models of C(3) photosynthesis can be used to predict that when photosynthesis is limited by the amount of Rubisco, increasing atmospheric CO(2) partial pressure (pCO(2)) will increase light-saturated linear electron flow through photosystem II (J(t)). This is because the stimulation of electron flow to the photosynthetic carbon reduction cycle (J(c)) will be greater than the competitive suppression of electron flow to the photorespiratory carbon oxidation cycle (J(o)). Where elevated pCO(2) increases J(t), then the ratio of absorbed energy dissipated photochemically to that dissipated non-photochemically will rise. These predictions were tested on Dactylis glomerata grown in fully controlled environments, at either ambient (35 Pa) or elevated (65 Pa) pCO(2), and at two levels of nitrogen nutrition. As was predicted, for D. glomerata grown in high nitrogen, J(t) was significantly higher in plants grown and measured at elevated pCO(2) than for plants grown and measured at ambient pCO(2). This was due to a significant increase in J(c) exceeding any suppression of J(o). This increase in photochemistry at elevated pCO(2) protected against photoinhibition at high light. For plants grown at low nitrogen, J(t) was significantly lower in plants grown and measured at elevated pCO(2) than for plants grown and measured at ambient pCO(2). Elevated pCO(2) again suppressed J(o); however growth in elevated pCO(2) resulted in an acclimatory decrease in leaf Rubisco content that removed any stimulation of J(c). Consistent with decreased photochemistry, for leaves grown at low nitrogen, the recovery from a 3-h photoinhibitory treatment was slower at elevated pCO(2).

Acclimatization↗

[Effect of elevated CO2 concentration on photosynthesis and antioxidative enzyme activities of wheat plant grown under drought condition].

The photosynthesis and antioxidative enzyme activities of wheat plants grown in two open-top chambers with CO2 concentrations of 350 mumol.mol-1 and 700 mumol.mol-1 were examined under drought stress. The result showed that elevated CO2 concentration obviously enhanced the photosynthesis, stomatal resistance and water use efficiency, but decreased the transpiration of wheat. Doubled CO2 concentration significantly increased the activities of CAT, POD and SOD, which enhanced the abilities of antioxidative defence and drought tolerance.

Carbon Dioxide↗

[Analysis on ecophysiological characteristics of leaf photosynthesis of Aneurolepidium chinense in songnen grassland].

A series of physiological characteristics of leaf photosynthesis and environmental factors are measured on Aneurolepidium chinense in Songnen salinized grassland during the growth season. The results showed that the daily change pattern of net photosynthetic rate (Pn) of Aneurolepidium chinense could be expressed as a two-humped curve, the daily change pattern of transpiration rate is one-humped curve. In addition, net photosynthetic rate (Pn), transpiration rate (E) and stomatal resistance (Rs) are affected by various environmental factors. At different growth stage, the influence of environmental factor on them is different, and even the same environmental factor had an different effect on Pn, E and Rs. In the whole growth period, photosynthetic available radiation (PAR) was the most intensive effect on the photosynthesis of Aneurolepidium chinense. Among Pn, E and Rs, E was one of the most obvious ecophysiological factor affected by environmental factors.

China↗

High light-induced switch from C(3)-photosynthesis to Crassulacean acid metabolism is mediated by UV-A/blue light.

The high light-induced switch in Clusia minor from C(3)-photosynthesis to Crassulacean acid metabolism (CAM) is fast (within a few days) and reversible. Although this C(3)/CAM transition has been studied intensively, the nature of the photoreceptor at the beginning of the CAM-induction signal chain is still unknown. Using optical filters that only transmit selected wavelengths, the CAM light induction of single leaves was tested. As controls the opposite leaf of the same leaf pair was studied in which CAM was induced by high unfiltered radiation (c. 2100 micromol m(-2) s(-1)). To evaluate the C(3)-photosynthesis/CAM transition, nocturnal CO(2) uptake, daytime stomatal closure and organic acid levels were monitored. Light at wavelengths longer than 530 nm was not effective for the induction of the C(3)/CAM switch in C. minor. In this case CAM was present in the control leaf while the opposite leaf continued performing C(3)-photosynthesis, indicating that CAM induction triggered by high light conditions is wavelength-dependent and a leaf internal process. Leaves subjected to wavelengths in the range of 345-530 nm performed nocturnal CO(2) uptake, (partial) stomatal closure during the day (CAM-phase III), and decarboxylation of citric acid within the first 2 d after the switch to high light conditions. Based on these experiments and evidence from the literature, it is suggested that a UV-A/blue light receptor mediates the light-induced C(3)-photosynthesis/CAM switch in C. minor.

Adaptation, Physiological↗

[Effect of light of different spectral composition on pigment accumulation, productivity and effectiveness of use of absorbed energy in photosynthesis during the developmental cycle of Chlorella].

The paper deals with the effect of light of different spectral composition on the duration of developmental cycle, pigment accumulation, productivity and efficiency of the use of absorbed energy in photosynthesis during the developmental cycle of Chlorella. An extremely high efficiency of using the green spectrum region in photosynthesis (21,0%) was observed. The efficiency of using absorbed radiation of the red (12%) and blue (6%) spectrum regions is also very high, but significantly lower than that of the green one. The activity of metabolic processes in Chlorella cells differs in various spectrum regions during different developmental cycles and is determined by the acting spectrum.

Chlorella↗

Differential susceptibility of photosynthesis to light-chilling stress in rice (Oryza sativa l.) depends on the capacity for photochemical dissipation of light.

The primary target for light-chilling stress in chilling-sensitive cucumber leaves is the chloroplast Cu,Zn-Superoxide dismutase, followed by subsequent inactivation of the photosystem (PS) I by reactive oxygen species (ROS). To test this hypothesis, two rice cultivars that were different in their ecological origins (a chilling-resistant Stejaree 45 and a chilling-sensitive Milyang 23) were evaluated with respect to photosynthetic properties, the ROS scavenging system, and expression of genes that are involved in sucrose synthesis and allocation upon the light-chilling stress. As expected, when the leaves were exposed to various low temperatures with illumination (150 micromol m(-2)s(-1)) for 6 h, the leaf photosynthesis of Milyang 23 decreased faster than that of Stejaree 45. The light-chilling induced differential photoinhibition of photosynthesis between the two cultivars was caused by the photoin-activation of PSII but not of PSI, since the potential quantum yield of PSII followed a similar trend to the changes in photosynthetic rates. The activities of the two chloroplastic antioxidant enzymes (superoxide dismutase and ascorbate peroxidase) that are known to be sensitive to oxidative stress were barely affected by the light-chilling treatments. Among various genes in sucrose metabolism (such as cytosolic FBPase, SPS, SUT, SuSy, and AGPase), the transcript levels of SuSy in Milyang 23 were significantly decreased by light-chilling stress compared to that of Stejaree 45. Based on these results, we propose that PSII, not PSI, is the sensitive site for light-chilling stress in chilling-sensitive rice. The extent of PSII photoinhibition depends on its capacity for the photochemical utilization of light.

Acclimatization↗

[Regulation function of calcium on photosynthesis of Dimocarpus longana Lour. cv. wulongling under simulated acid rain stress].

Studies on the regulation function of calcium on photosynthesis of Dimocarpus longana under simulated acid rain stress showed that the photoreduction activity of chloroplasts was activated when the concentration of calcium ion in reaction medium ranged from 0 to 5 mmol.L-1, and peaked at the 3.5 mmol.L-1, which was 41.90% higher than that of control. Conversely, the activity of chloroplasts reduced 26.06% in the reaction medium with a concentration of 2 mmol.L-1 EGTA, as compared with the control. Both Mn2+ and Mg2+ could inhibit photoreduction activity. The photophosphorylation activity increased when the concentration of calcium ion in reaction medium ranged from 0 to 6 mmol.L-1, and peaked at the 4.5 mmol.L-1, while superoxidase dismutase (SOD) activity rose from 0 to 6 mmol.L-1 and peaked at 6 mmol.L-1. Calcium ion with the concentration of both 10 mmol.L-1 and 15 mmol.L-1 could increase the content of chlorophyll(Chl), stabilize the membrane structure of leaf discs, and reduce the membrane permeability under simulated acid rain with pH value of 3.0. The effect in 15 mmol.L-1 were better than in 10 mmol.L-1. However, the injury of acid rain to leaves was strengthened when the concentration of calcium was higher than 20 mmol.L-1. Net photosynthesis rate (Pn) rose when leaves sprayed with 15 mmol.L-1 Ca(NO3)2 before treatment of acid rain stress of pH 2.5. All of the results represented the excellent protection function of calcium on D. longana leaves under simulated acid rain.

Acid Rain↗

[Simulating photosynthesis and evapotranspiration of winter wheat with a SVAT model].

Two sub-models were added to the developed soil-vegetation-atmosphere transfer model. Of the two sub-models, one was the canopy photosynthesis/stomatal coupled sub-model distinguishing the sun light and shaded leaves, and the second was the simplified growth sub-model considering growth respiration and maintenance respiration. The comparison of measured and simulated daily evapotranspiration showed that the RMSD (root mean standard deviation) of model prediction to observation was 0.65 mm, and MAPD (mean absolute percent difference) was 14%. The daily course of the simulated and measured photosynthesis rates at the top of canopy were in good consistency. With the high-resolution meteorological data, the canopy net photosynthesis and evapotranspiration were simulated over the whole growing season. The sensitivities of maximum leaf area index, environmental CO2 concentration and leaf nitrogen content to evapotranspiration and water use efficiency were also analyzed.

Ecosystem↗

Antagonistic reactions of arginine and lysine against formaldehyde and their relation to cell proliferation, apoptosis, folate cycle and photosynthesis.

1H, 13C NMR, ESMS and MS/MS investigations proved that there is an antagonism in the spontaneous reaction of formaldehyde with L-lysine and L-arginine. L-Arginine can only be hydroxymethylated on the guanidino group in a very fast reaction forming mono-, di-, and trihydroxymethyl arginines (HMA). L-Lysine can be methylated on the epsilon-amino group forming mono-, di-, and trimethyl lysine on physiological pH. Hydroxymethyl arginines are relative stable, isolable products, and can also be formed in biological systems, especially in plants. Significant amounts of hydroxymethyl arginines were identified in the aqueous extract of lyophilized kohlrabi, which can be formed in photosynthesis during CO2 fixation. 14C-Formaldehyde formed in a short-term (10, 30 sec) 14CO2 fixation reaction in Zea mays L. (early maturity variety: Szegedi TC 277) was captured by L-arginine, which occurs in leaves in large amount. Formaldehyde formed during photosynthesis can react not only with the arginine, but with ribulose-1,5-diphosphate present in leaves. In model reactions formaldehyde can react with the 'ene diole' group of ribulose-1,5-diphosphate in the absence of Rubisco enzyme, which is a similar reaction to the addition of formaldehyde to L-ascorbic acid. Hydroxymethyl arginines (HMA) are endogenous formaldehyde carrier molecules transferring the bound formaldehyde to thymidylate synthase enzyme system incorporating it into the folate cycle. HMA can also carry the bound formaldehyde to the cells especially to the tumorous cells (HT29 adenocarcinoma), and cause significant inhibition of cell proliferation and causes apoptosis.

Apoptosis↗

Photosynthesis: a short history of some modern experimental approaches.

This paper presents a personal interpretation of a chapter of plant physiology beginning from the early 1930s to the early 1940s, when plant physiologists tried to find the missing link between the two (dark and light) phases of photosynthesis. As initially inferred by Richard Willstätter and Arthur Stoll in the 1910s, and then stated by Robert Emerson and William Arnold in 1932, the most accredited theory proposed that carbon dioxide must combine with chlorophyll in the dark. Successive light flashes activated the complex chlorophyll-carbon dioxide with oxygen evolution, and carbon dioxide was reduced to formaldehyde and successively polymerised into hexose. Arthur Stool in 1932 and Cornelius v. Niel in 1935 gave the first stroke to this theory suggesting that carbon dioxide must be reduced and assimilated by means of a process of water oxidation. Robert Hill showed the existence of an indissoluble link between the light phase, water oxidation and possibly oxygen evolution. Two physicists, Sam Ruben and Martin Kamen proposed the assembly of photosynthesis into a unitary process occurring as a sequence of several steps in the first 1940s. By utilising for the first time radioactive carbon (11C), they elaborated a new theory according to which carbon dioxide reduction was a repeated "cyclic" mechanism. This "heretical" view abolished the old, but still considered, theory of formaldehyde. Hill, Ruben and Kamen were able to exploit at best the possibility offered by a very advanced technology, thus confirming once again that ideas stand upon the powerful legs of technology.

Botany↗

Influences of excessive Cu on photosynthesis and growth in ectomycorrhizal Pinus sylvestris seedlings.

Growth and photosynthesis responses were measured for Scots pine (Pinus sylvestris L. cv.) inoculated with ectomycorrhizal fungi (Suillus bovinus) under 6.5 and 25 mg/L Cu treatments to evaluate ectomycorrhizal seedlings' tolerance to heavy metal stress. Results showed that excessive Cu can significantly impair the growth and photosynthesis of pine seedlings, but such impairment is much smaller to the ectomycorrhizal seedlings. Under 25 mg/L Cu treatment, the dry weight of ectomycorrhizal seedlings is 25% lower than the control in contrary to 53% of the non-mycorrhizal seedlings, and the fresh weight of ectomycorrhizal roots was significantly higher than those of non-mycorrhizal roots, about 25% and 42% higher at 6.5 and 25 mg/L Cu treatments respectively. Furthermore, ectomycorrhizal fungi induced remarkable difference in the growth rate and pigment content of seedlings under excessive Cu stress. At 25 mg/L Cu, the contents of total chlorophyll, chlorophyll-a and chlorophyll-b were 30% higher in ectomycorrhizal plants than those in non-mycorrhizal plants. O2 evolution and electron transport of PSI and PSII were restrained by elevated Cu stress. However, no significant improvement was observed in reducing the physiological restraining in ectomycorrhizal seedlings over the non-mycorrhizal ones.

Chlorophyll↗

[Effect of hormones on regulating and controlling photosynthesis rate of wheat nag leaf at their different development phases].

By the method of ELASA, this paper measured the ABA/ZRs ratio in wheat flag leaf at the relative steady phase (RSP), end steady phase (ESP) and sharply descending phase (SFP) of chlorophylls. The ABA, ZRs and their mixture were led to the transpiration current of plant to measure the changes of photosynthesis rate at these three phases. The results showed that the ABA/ZRs ratio in flag leaf was 4.20, 41.83 and 14.40 at RSP, ESP and SFP, respectively. The time that the photosynthesis rate reduced by ABA to zero was 49.5, 39.1 and 38.0 min, and that reduced by ZRs to half was 65, 49 and 31 min, respectively. The two preceding phases could be regulated, and the changes were reversible.

Abscisic Acid↗

[Effects of nitrate application on alleviating photosynthesis restriction of Cinnamomum burmannii leaves under elevated CO2 concentration and enhanced temperature].

In this study, potted C. burmannii saplings were cultured in a top-closed chamber with elevated CO2 (+ CO2, 731 micromol x mol(-1)) and ambient CO2(CO2, 365 micromol x mol(-1)), and at diurnal temperature (day/night) 25/23 degrees C and 32/25 degrees C, respectively. The gas exchange, calculated photosynthesis parameter, Rubisco content, and activated state of Rubisco were examined. The results showed that under + CO2 and at 25/23 degrees C, the mean photosynthetic rate (Pnsat) of sapling leaves was 5.1% higher than that under + CO2 and at 32/25 degrees C. Temperature enhancement declined Pnsat, while nitrate addition increased it. Under + CO2, saplings had lower V(cmax) and J(max) at 32/25 degrees C than at 25/23 degrees C. Temperature enhancement under + CO2 also declined V(cmax) and J(max). Under + CO2, lower photorespiration rate (Rp) occurred in leaves, but temperature enhancement could increase Rp. Under + CO2, Rubisco content (NR) and its active site per unit leaf area (M) decreased as diurnal temperature changed from 25/23 degrees C to 32/25 degrees C. Meanwhile, nitrate addition could increase NR and M. It may be suggested that nitrate addition could alleviate the restriction of photosynthesis under elevated CO2 concentration and enhanced temperature.

Carbon Dioxide↗