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At least 703 records · Page 39Linked to original sources
PubMed1987

Anatomic correlation of cadaver cryomicrotomy with magnetic resonance imaging.

The performance of frozen sections of a thickness varying between 5 and 50 microns in fresh undecalcified cadaveric human specimens was perfected in Sweden by one of the authors in 1983. This technique makes it possible to obtain anatomic images of high definition which were correlated with MRI sections made at intervals of 20 microns.

Cadaver↗
PubMed1987

Cellular junctions on the free surface of human placental syncytium.

Chorionic villi of human placenta at full term have been studied in thin section. On the free surface of syncytium, cellular junctions such as desmosomes, desmosome-like junctions and tight junctions, but not gap junctions, are present. Theories regarding the formation and functions of the junctions on the syncytial free surface are discussed.

Chorionic Villi↗
PubMed1982

A technique for cutting slices from femoral heads and other awkwardly shaped bones.

For cutting thin, parallel slices from round and other awkwardly shaped bones, we have developed an useful aid by modifying the Microslice II cutting saw primarily designed for geological or metallurgical purposes. To ensure a good grip of the bone, the specimen is embedded in a bolus of wax in a cylindrical container and is advanced along the container by a screw plunger toward the cutting blade of the machine. It is thus possible to cut even from awkwardly shaped bones 2-30 mm thick parallel slices suitable for decalcified or undecalcified histological sections and also for radiodensitometry or quantitative microradiography.

Bone and Bones↗
PubMed1997

Plastination of the larynx for whole-organ sectioning.

Whole-organ sectioning is an important technique for the assessment of laryngeal pathology. Since currently established methods require prior decalcification which causes morphological changes, the critical border area between cartilage and surrounding soft tissue cannot be investigated in the same specimen and morphometric studies are not possible. Plastination is a laboratory technique that has previously demonstrated its capacity to overcome these shortcomings. In so doing water and lipids are replaced by curable polymer within the laryngeal cells making decalcification unnecessary. In the present study, more than 50 human larynges were processed using block plastination (BP) and sheet plastination (SP). For BP the complete organ was plastinated as a whole and then cut into thin serial sections. For SP the fresh organ was sliced first and plastinated in a second step. Findings demonstrated that SP allowed for the production of whole-organ sections within a period of 1 week only. Section thicknesses were as thin as 15 mm using a diamond wire saw and an ultramilling device. Sectioning was possible in both coronary and horizontal planes. Following BP, specimens were cut in an industrial cutting machine to thicknesses of about 0.6 mm. Shrinkage of tissue was less than 10% for both methods. In all, SP was technically superior to routine paraffin histology, although cutting equipment is very expensive and delicate in handling. At present the technique of BP is the method of choice for macromorphometrical investigations on serial sections of the human larynx.

Acetone↗