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PubMed2026

Genomic insights into an optrA-carrying plasmid associated with linezolid resistance in clinical Enterococcus faecalis isolates, Argentina.

The spread of the transferable optrA gene poses an increasing threat to the clinical efficacy of oxazolidinones. Here, we characterized a novel optrA-carrying plasmid, pEfa-optrA-Arg, from a linezolid-resistant Enterococcus faecalis clinical isolate from Argentina. The 68,653-bp conjugative plasmid harbored optrA together with multiple antimicrobial resistance genes and showed high similarity to a plasmid previously identified in a bovine isolate from Switzerland. pEfa-optrA-Arg, or a closely related variant, was also detected in E. faecalis isolates from several Argentinian hospitals, highlighting the role of horizontal gene transfer in the spread of antimicrobial resistance across human and animal reservoirs within the One Health continuum.

Enterococcus faecalis↗
PubMed1998

Treatment options for chronic prostatitis due to vancomycin-resistant Enterococcus faecium.

Prostatitis due to vancomycin-resistant enterococci has not been previously described. Reported here is a case of chronic prostatitis due to Enterococcus faecium, resistant to vancomycin, ampicillin, ciprofloxacin and doxycycline, in a 42-year-old liver transplant recipient. Treatment with a combination of rifampin and nitrofurantoin for 6 weeks resulted in long-lasting cure. Other antimicrobial agents active in vitro against vancomycin-resistant enterococci, such as quinupristin/ dalfopristin and chloramphenicol, are unlikely to achieve sufficient prostatic tissue levels to be successfully utilized for treatment of this condition.

Adult↗
PubMed2003

Meningitis caused by Enterococcus gallinarum in patients with ventriculoperitoneal shunts.

Vancomycin-resistant enterococci have recently emerged as significant nosocomial pathogens. Here we describe two Japanese patients, a 57-year-old man and a 12-year-old boy, with ventriculoperitoneal shunts for hydrocephalus who developed meningitis caused by vancomycin-resistant Enterococcus gallinarum. The infection of the central nervous system in these two patients may have been associated with E. gallinarum derived from the gut. Removal of the shunts and antimicrobial treatment promoted apparent improvement in these patients. The risk factors and management of vancomycin-resistant enterococcal infections in the central nervous system are discussed.

Bacterial Proteins↗
PubMed2006

Batch and repeated batch production of L (+)-lactic acid by Enterococcus faecalis RKY1 using wood hydrolyzate and corn steep liquor.

Lactic acid production was investigated for batch and repeated batch cultures of Enterococcus faecalis RKY1, using wood hydrolyzate and corn steep liquor. When wood hydrolyzate (equivalent to 50 g l(-1) glucose) supplemented with 15-60 g l(-1) corn steep liquor was used as a raw material for fermentation, up to 48.6 g l(-1) of lactic acid was produced with, volumetric productivities ranging between 0.8 and 1.4 g l(-1 )h(-1). When a medium containing wood hydrolyzate and 15 g l(-1) corn steep liquor was supplemented with 1.5 g l(-1) yeast extract, we observed 1.9-fold and 1.6-fold increases in lactic acid productivity and cell growth, respectively. In this case, the nitrogen source cost for producing 1 kg lactic acid can be reduced to 23% of that for fermentation from wood hydrolyzate using 15 g l(-1) yeast extract as a single nitrogen source. In addition, lactic acid productivity could be maximized by conducting a cell-recycle repeated batch culture of E. faecalis RKY1. The maximum productivity for this process was determined to be 4.0 g l(-1 )h(-1).

Biomass↗
PubMed2005

Copper chaperone cycling and degradation in the regulation of the cop operon of Enterococcus hirae.

Extensive insight into copper homeostasis has recently emerged. The Gram-positive bacterium Enterococcus hirae has been a paradigm for many aspects of the process. The cop operon of E. hirae consists of four genes that encode a repressor, CopY, a copper chaperone, CopZ, and two CPx-type copper ATPases, CopA and CopB. CopA and CopB accomplish copper uptake and export, respectively, and the expression of the cop operon is regulated by copper via the CopY repressor and the CopZ chaperone. The functions of the four Cop proteins have been extensively studied in vivo as well as in vitro and a detailed understanding of the regulation of the cop operon by copper has emerged.

Adenosine Triphosphatases↗
PubMed2006

Distribution of four capsular serotypes of Enterococcus faecalis among clinical isolates from different geographical origins and infection sites.

BACKGROUND: Enterococci possess capsular polysaccharide antigens that are the targets of opsonic antibodies. These antibodies are potential candidates for development as immunotherapy. MATERIAL AND METHODS: The present study analyzes the distribution of four capsular serotypes within a collection of 157 isolates of Enterococcus faecalis from four countries with different sites of clinical infection. RESULTS: By using a capsular polysaccharide-specific ELISA, 42% of the isolates were grouped into one of four serogroups, and another 9% showed cross-reactivity between two serotype-specific sera. Heterogeneity of serotype distribution by both geographical origin and infection site was observed. CONCLUSION: Half of the strain collection could be typed with four serotype-specific sera. No serotype from a given country or infection site clearly predominated.

Bacterial Capsules↗
PubMed1992

Conditions modulating the ionic selectivity of transport by monensin examined on Enterococcus hirae (Streptococcus faecalis) by 23Na-NMR and K+ atomic absorption.

Factors likely to modulate the ionic selectivity of monensin were examined on Enterococcus hirae (Streptococcus faecalis) in two states previously characterized: the resting (de-energized) cell and the active (energized) cell. Internal and external Na+ were followed by corresponding 23Na-NMR resonances K+ concentrations were measured by atomic absorption. For a given cellular population of de-energized cells, the apparent transport rates and the final cationic concentrations reached at the steady state were decreasing with the ionophore dose. Monensin was selective for sodium only at low concentrations, in the range 1 mM-10(-4) mM the transport was depending on the effective cationic gradients. Comparison of the activity curves for two cell populations (7.10(9) and 7.10(10) cells/ml) showed the importance of the ratios of monensin/mg phospholipid and also of the ratios of external/internal volumes. On energized cells, except for low monensin concentrations, the main effect was a K(+)-induced efflux and not a Na+ influx. Two factors were modulating the resulting selectivity of this ionophore: the response of the intrinsic bacterial carriers and the generation of the gradients (mainly the external pH) which were favourable to a K+/Na+ transport. Once again the results obtained for two cell populations could be compared, the determining factors were the ratio external/internal volume and the generation of the pH gradient.

Biological Transport↗
PubMed1990

Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1.

The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-BglII 3.6-kbp fragment of the plasmid pAM351 (pPD1::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gln-Gln-Pro repeat in the C-terminal region of TraD product encoded on the R100 plasmid in Escherichia coli.

Amino Acid Sequence↗
PubMed1990

Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1.

The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-BglII 3.6-kbp fragment of the plasmid pAM351(pPdl::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gln-Gln-Pro repeat in the C-terminal region of TraD product encoded on the R100 plasmid in Escherichia coli.

Amino Acid Sequence↗
PubMed1991

Primary structure of the alpha-subunit of vacuolar-type Na(+)-ATPase in Enterococcus hirae. Amplification of a 1000-bp fragment by polymerase chain reaction.

A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that the amino acid sequence of Na(+)-ATPase alpha-subunit is highly homologous to the corresponding sequences of large (alpha) subunits of vacuolar (archaebacterial) type H(+)-ATPases, supporting our proposal [Kakinuma, Y. and Igarashi, K. (1990) FEBS Lett. 271, 97-101] that the Na(+)-ATPase of this organism belongs to the vacuolar-type ATPase.

Adenosine Triphosphatases↗
PubMed1995

Electrogenic Na+ transport by Enterococcus hirae Na(+)-ATPase.

Energy-dependent generation of a membrane potential (delta psi) (-45 mV, interior negative) was observed in the F0F1, H(+)-ATPase-defective mutant of Enterococcus hirae. The generation of delta psi was found at high pH (but not at low pH), for which intracellular Na+ was required but not extracellular K+. The delta psi-generating activity was induced in cells cultured in media containing high concentrations of Na+, and was not observed in the Na(+)-ATPase mutants. These results suggest that E. hirae Na(+)-ATPase is responsible for the electrogenic sodium pump.

Adenosine Triphosphatases↗
PubMed1989

Diagnosing endocarditis with the cloned 112 kDa antigen of Enterococcus faecalis.

A new indirect ELISA is presented for the diagnosis of enterococcal endocarditis. It is based on the cloning of Enterococcus faecalis DNA into lambda gt11. The library was screened by antisera from three cases of E. faecalis endocarditis. Three positive clones were found, all of which cross-reacted with the 112 kDa antigen of E. faecalis. One of these was taken and lysogenised into E. coli Y1089 to produce a fusion protein of 125 kDa. This IPTG dependent protein was purified by affinity chromatography and used in an indirect ELISA. The test differentiated between five cases of enterococcal endocarditis (IgG ELISA optical density greater than 0.8) and patients with endocarditis due to staphylococci (IgG ELISA optical density less than 0.243) and other streptococci (IgG ELISA optical density less than 0.656). Patients with a simple E. faecalis septicaemia had a maximum IgG ELISA optical density of 0.636. The only major cross-reaction occurred in patients with Streptococcus bovis endocarditis.

Antibodies, Bacterial↗
PubMed1994

Pathogenicity of Enterococcus hirae for chicken embryos and betamethasone-treated chicks.

An isolate Enterococcus hirae was used to determine its pathogenicity for chicken embryos and for chicks treated with betamethasone. E hirae was inoculated intravenously into four-day-old chicks which had been treated for three consecutive days with betamethasone, and chick embryos were inoculated into the allantoic cavity with 10(2) and 10(3) bacteria. E hirae was not pathogenic for the chicks or for the embryos.

Allantois↗
PubMed1989

Sex pheromones and plasmid transfer in Enterococcus faecalis.

Plasmid-free Enterococcus faecalis excrete peptides (sex pheromones) which specifically induce a mating response in strains harboring certain conjugative plasmids. The response is characterized by the synthesis of a "fuzzy" surface material, visible by electron microscopy, which is believed to facilitate the aggregation of donors and recipients. Transconjugants which receive a specific plasmid shut down the production of endogenous pheromone; however, they continue to produce pheromones specific for donors harboring different classes of plasmids. In this review, we summarize what is known about the biochemistry and genetics of this phenomenon. Some emphasis is given to the hemolysin plasmid pAD1 and the regulation of its conjugal transfer.

Amino Acid Sequence↗
PubMed1990

Mutants of Enterococcus faecalis deficient as recipients in mating with donors carrying pheromone-inducible plasmids.

From Enterococcus faecalis cells containing random chromosomal insertions of Tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. The phage-resistant strains all showed decreased recipient ability (Con-) in broth matings with donors carrying pheromone-inducible plasmids. These strains were normal with respect to donor ability in broth matings and recipient ability in filter matings. The data suggest that the mutants are deficient in the binding substance receptor for the pheromone-induced donor aggregation substance. These mutants contained multiple insertions of Tn916, and none of the individual insertions from the mutant strains were capable of generating the phenotype. Analysis of cell envelope lipoteichoic acids and protein revealed changes in both associated with the Con- phenotype.

Bacteriophages↗
PubMed1991

Control of Enterococcus faecalis sex pheromone cAD1 elaboration: effects of culture aeration and pAD1 plasmid-encoded determinants.

Aeration of plasmid-free Enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cAD1 activity in culture filtrates. Levels of two unrelated pheromones, cPD1 and cAM373, were unaffected by culture aeration. Aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pAD1 traB mutations, verifying a link between traB function and pheromone "shutdown." Tests with a series of pAD1 mini-plasmids indicated that the product of the traB gene was involved in, but not sufficient for, pheromone shutdown; the cooperation of one or more other gene products encoded within the pheromone response control region was required.

Aerobiosis↗
PubMed1991

A phase variation event that activates conjugation functions encoded by the Enterococcus faecalis plasmid pAD1.

Enterococcus faecalis cells carrying the conjugative plasmid pAD1 undergo several related changes when induced by the sex pheromone cAD1. Included are the production of novel surface proteins, the formation of cellular aggregates in broth cultures, the ability to transfer the plasmid at high frequency in broth matings, and the change from a soft to a "dry" colony morphology. Spontaneous, constitutively dry colony (Dryc) variants of E. faecalis (pAD1) were found to arise at a frequency of 10(-4)-10(-2). Dryc phase variants constitutively expressed aggregation and plasmid transfer functions typically expressed only under cAD1-inducing conditions. Reversion of Dryc variants to a cAD1-inducible phenotype (Dry+) occurred at a similar frequency. Tn917-lac mutagenesis of regions of pAD1 previously shown to be involved in plasmid transfer revealed that in Dry+ cells these regions were transcribed only when the inducer, cAD1, was present. In Dryc variants the regions were transcribed constitutively. A pAD1 miniplasmid containing determinants regulating cAD1 inducible plasmid transfer and a cAD1-inducible lacZ transcriptional fusion displayed phase variation in LacZ expression at a rate similar to the Dry+/Dryc phase variation. These results suggest that the site of mutation(s) resulting in the Dryc phenotype is within the regulation-related region of pAD1. Complementation tests showed that this region, when supplied in trans, complemented the Dryc phenotype. Phase variation affecting mating functions represents an alternative (pheromone independent) method of regulating pAD1 transfer.

Conjugation, Genetic↗