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At least 703 records · Page 39Linked to original sources
PubMed2003

Determination of thiocolchicoside in its binary mixtures (thiocolchicoside-glafenine and thiocolchicoside-floctafenine) by TLC-densitometry.

The proposed method is based on TLC separation of thiocolchicoside from its binary mixtures (thiocolchicoside-glafenine and thiocolchicoside-floctafenine) followed by densitometric measurement at 375 nm. Separation was carried out on silica gel plates GF(254) using ethyl acetate:methanol:acetic acid (84:13:3%, v/v/v). Various conditions affecting separation and measurement were studied and optimized. Calibration was performed using third-order polynomial equation. It was found superior to first-order with respect to quantification range (0.25-25 microg per spot), correlation coefficient and standard error of estimation. The proposed method was successfully applied for the determination of thiocolchicoside in its synthetic binary mixtures and commercial tablets. Results were compared with those obtained by reference methods and non-significant difference was obtained regarding accuracy and precision. Assay precision using two-way ANOVA was performed on results of inter- and intra-day applications of the method.

Chromatography, Thin Layer↗
PubMed1978

Quantitative estimation of salicylic acid and its metabolites by thin-layer densitometry.

A rapid thin-layer densitometric method for the quantitative determination of salicylic acid and its metabolites in urine or plasma is described. The method is specific and very sensitive. Nanogram quantities of salicylic acid and its metabolites, both free and conjugated, may be estimated. Known metabolites, as well as the newly described gentisuric acid, were estimated quantitatively in urine from a patient treated with aspirin.

Aspirin↗
PubMed1977

Erythromycin series. V. Quantitative analysis of cladinose and methylcladinoside by densitometry of thin-layer chromatograms.

A direct, quantitative, thin-layer chromatographic method is described for the determination of sugar cladinose and methylcladinoside in the presence of other acid-degradation products of the antibiotics erythromycin oxime and erythromycyl-amine. Cladinose and methylcladinoside are separated from compounds which cause interference on pre-coated silica gel F254 plates, and are measured directly on the thin-layer plate using a densitometer. Standard graphs obtained for cladinose and methylcladinoside show a linear relation between the square root of the peak area and the logarithm of the amount of substance present in the spot, as well as between the square of the area and the logarithm of the amount. This method is very successful in stability studies on the antibiotics erythromycin oxime and erythromycylamine in an acid medium. The technique seems to be particularly useful in instances in which the usual analytical methods either cannot be applied or can be applied only with difficulty.

Chromatography, Thin Layer↗