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The pathology induced in laboratory rats by metacestodes of Taenia crassiceps and Mesocestoides corti (Cestoda).

Sprague-Dawley rats were infected by intraperitoneal injection with either the cysticerci of Taenia crassiceps or the tetrathyridia of Mesocestoides corti. Both species of metacestodes reproduce asexually in the peritoneal cavity. The effect of T. crassiceps upon the rats was to cause chronic inflammation of the peritoneum, fibrous adhesions between the visceral organs and hyperplasia of the gut mesenteries which became extended into a large sac-like structure. Dead parasites surrounded by white calcareous material were found free in the peritoneum and attached to the inner body wall and viscera. The histopathology of the hyperplastic mesenteries and fibrous membrane is described. The tetrathyridia of M. corti were found free in the peritoneal cavity as well as in the liver (mainly the left liver-lobe), gut mesenteries and in an outgrowth of the connective tissue associated with the pancreas. The connective tissue outgrowth comprised 2 layers; an inner reticular layer and a thickened marginal layer. Metacestodes were found in collagen-fibrin capsules within the connective tissue. A layer of mucopolysaccharide material was interposed between the parasite and the capsule wall. A possible method by which the parasites gain entry into the connective tissue is described. The connective tissues of rats harbouring either M. corti or T. crassiceps had increased numbers of mast cells compared to connective tissues from control rats. Younger mast cells containing biogenic amines predominated in infected tissues whereas older heparin-containing cells dominated control tissues.

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Tapeworm infection is a significant risk factor for spasmodic colic and ileal impaction colic in the horse.

The association between the equine intestinal tapeworm Anoplocephala perfoliata and specific types of intestinal disease was investigated by matched case-control study using coprological and serological diagnosis. We have previously shown that the host IgG(T) response to 12/13 kDa antigens of A. perfoliata correlates well with infection intensity, therefore this antibody response was used to investigate the risk of colic at different levels of parasite infection intensity. One hundred and three spasmodic colic cases with an equal number of controls matched for age, breed and gender, and 20 ileal impaction cases each with 2 similarly matched controls were obtained. Cases of spasmodic colic were much more likely (odds ratio = 8.0) to be associated with A. perfoliata infection detected coprologically than controls. Serological diagnosis revealed an increasing risk of spasmodic colic with increasing infection intensity. Calculation of an aetiological fraction suggests that 22% of spasmodic colic cases in this study were tapeworm associated. No significant association was found between colic and strongyle egg count. Conditional logistic regression analysis demonstrated that the relationship between colic and A. perfoliata infection intensity was not confounded by strongyle egg count and there was a linear relationship between infection intensity and the log-odds of spasmodic colic. For cases of ileal impaction, a strong association was found between colic and A. perfoliata as diagnosed by coprological means (odds ratio of 34.0). Serological diagnosis also revealed a strong association that increased with higher levels of infection intensity (odds ratio = 26.0). The aetiological fraction for the ileal impaction data suggests that 81% of the ileal impaction cases in this study were tapeworm associated. This study concludes that A. perfoliata is a significant risk factor for spasmodic colic and ileal impaction colic in the horse; and that the risk of spasmodic colic increases with infection intensity.

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Long-term follow-up of the surgical treatment of intracranial coenurosis.

Intracranial infestation by the coenurus of the tapeworm Taenia multiceps is a rare occurrence in humans, with about 55 cases having been reported so far. Although most of these cases were observed in African or South American countries, the illness was occassionally found also in sheep-raising areas of Western Europe and the United States. Out of 4 personal cases, who were operated upon over an 18-year-period, 2 were neurologically intact respectively 20 and 3 years following surgical treatment. CT scans confirmed the absence of a progression of the disease. One patient was able to work 6 years after surgery, when he was killed in a road accident. A further, severely impaired patient remained unchanged and died with intercurrent infection 10 years following the operation.

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Second case of human infection with Mesocestoides lineatus in Korea.

The second case of human infection with Mesocestoides lineatus in Korea was reported. The patient, a farm worker, complained of abdominal pain and massive discharge of sesame-like proglottids in his stool for several months. Worms, recovered by chemotherapy with niclosamide, consisted of 32 strobilae. This may be the heaviest worm burden in human infection ever reported. The infected man habitually ate the raw viscera of chickens.

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A survey into the prevalence of poultry helminths in northwest Arkansas commercial broiler chickens.

A 12-month survey was conducted to determine the prevalence of poultry helminths in Northwest Arkansas commercial broiler chickens. Intestinal tracts from market-ready broilers were collected weekly from two commercial broiler companies; a total of 3542 intestinal tracts were collected for parasite enumeration from 67 company A and 52 company B farms. Ascaridia galli was found on 37.3% of company A farms and 3.9% of company B farms, Raillietina cesticillus was found on 67.2% of company A farms and 69.2% of company B farms, and Heterakis gallinarum was found on 7.5% of company A farms and 1.9% of company B farms. No other parasitic helminths were found. No significant relationship was detected between grow-out feed efficiency and helminth infection rate or magnitude.

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[Changes in the argyrophil stroma of the liver of fishes infested with the pleurocercoids, Triaenophorus nodulosus and Diphyllobothrium dendriticum (Cestoidea, Pseudophyllidae)].

The argentaffine stroma of the liver of some fishes (Perca fluviatilis, Exos lucius, Paracottus kessleri, Coregonus autumnalis migratorius, Thymallus arcticus) is described. The nature and depth of pathological changes in the argentaffine stroma of the liver at its infection with pleurocercoids of pseudophyllids depend on the infection intensity, host's specificity and on the biocoenotic relationships of hydrobionts in a waterbody.

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Host specificity in mice selected for innate immunity to Nematospiroides dubius: infections with Nippostrongylus brasiliensis, Mesocestoides corti and Salmonella typhimurium.

The selection of mice for innate immunity to Nematospiroides dubius was not specific. Mice bred as refractory (R) to N. dubius infection were more refractory to primary infections with Nippostrongylus brasiliensis than other mice bred as liable (L) to infection with N. dubius. R and L, as well as randomly-bred (Rd) and inbred C3H mice were all immune to challenge infection with N. brasiliensis. Previous infections with N. brasiliensis failed to influence the course of N. dubius infections or the status of the selected mice as R, Rd and L to infection with N. dubius. R and L mice were equally susceptible to Mesocestoides corti infection but more resistant than Rd mice. R and L mice died sooner after infection with Salmonella typhimurium than Rd, although R survived longer than L mice.

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MCP-1 and MIP-2 levels during Echinococcus granulosus infections in mice.

Ten BALB/c mice were infected with the cestode Echinococcus granulosus. After the infection, serum was collected at different periods of time and monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) were determined. The level of MCP-1 increased from at day 20 post infection (p.i.), to a maximum of on day 60 p.i., then decreased to on day 130 p.i. A second peak was observed at day 150 p.i. In addition, MIP-2 was detectable in serum as late as day 100 p.i. The highest level was observed on day 130 p.i., and decreased thereafter. Serum from noninfected animals (controls) contained no detectable levels of either MCP-1 or MIP-2. However, MCP-1 and MIP-2 appear to be implicated in E. granulosus infections, but their exact role during the disease is under determination.

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Intestinal helminths of four species of skinks (Mabuya) (Sauria: Scincidae) from southern Africa.

Intestinal helminths are reported from four species of scincid lizards from southern Africa: Mabuya occidentalis, Mabuya spilogaster, Mabuya striata and Mabuya variegata. The helminth fauna consisted of one species of Cestoda, Oochoristica truncata and five species of Nematoda, Abbreviata paradoxa, Maxvachonia dimorpha, Parapharyngodon rotundatus, Spauligodon petersi and Thubunaea fitzsimonsi. All findings represent new host records. Ascarid larvae were also found. It appears that Mabuya is infected by generalist helminths that occur in other species of African lizards.

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Urinary bladders of freshwater turtles as a renal physiology model potentially biased by monogenean infections.

Because the urinary bladder of the freshwater red-eared slider turtle, Trachemys scripta elegans, is analogous to the collecting and distal tubules of the mammalian kidney, it is used frequently as an experimental model in renal physiology. The urinary bladders of 41% of these turtles obtained over a 1 year period from a commercial supplier were infected with the monogenean parasite Neopolystoma orbiculare. Macroscopically, the infected bladders appeared normal, aside from the presence of adult parasites (means = 2.3 +/- 0.3 monogeneans/turtle). However, there were distinct histological lesions associated with the deposition of masses of monogenean eggs in the bladder wall. Clusters of the eggs in varying stages of development in the mucosa and tunica muscularis were surrounded by granulomas with an inflammatory infiltrate of histiocytes, lymphocytes and occasional multinucleate giant cells. These often formed granulomatous polyps projecting into the bladder lumen. Eggs containing a mature oncomiracidium are apparently extruded into the bladder lumen. In general, the bladder walls of infected turtles were infiltrated with eosinophils and lymphocytes. Considering the extent of the inflammatory lesions, it is recommended that turtle bladders infected with these parasites not be used as models for renal physiology until the effects of these infections on the various physiological measurements are determined.

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Metazoan parasites of Himantopus mexicanus Muller (Aves) from southwestern Texas, with a checklist of helminth parasites from North America.

Nineteen species of helminths were recovered from 34 of 35 black-necked stilts, Himantopus mexicanus Muller, collected from the Fort Bliss ponds, El Paso County, Texas. New host records are marked with an. The species identified were: Acoleus vaginatus, Davainea himantopodis, Diplophallus polymorphus, Eurycestus avoceti, Hymenolepis himantopodis, Hymenolepis sp. 1, Infula macrophallus, Coacitrema michiganensis, Cyclocoelum lanceolatum, Notocotylus sp., Parastrigea mexicanus, Tanaisia fedtschenkoi, Capillaria sp., C. anatis, C. contorta, C. mergi, Chevreuxia americana, Eustronglydes mergorum, and Splendidofilaria sp. Six species of mallophagan lice and 1 species of nasal mite, Rhinonyssus himantopus, were recovered. Helminths showed little concentration for dominance (0.09), were not very evenly distributed (0.49 +/- 0.08) nor very diverse (0.73 +/- 0.14), and most species were highly aggregated. The helminth community consisted of an unusually large number of core species (10). Three large species of tapeworms exhibited mostly paired infections, were mutually exclusive, and were negatively associated (-1).

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