SEARCH · Search PubMed
Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Samples derived from farm livestock, an abattoir and a bone factory, were examined for salmonella. Twenty-seven serotypes were detected in 130 infected samples. A bone factory product was heavily infected. Salmonellosis is considered to be an important disease of dromedary calves and poultry in Ethiopia.
The main purpose of this work was the preliminary qualitative study of organic compounds in wastewaters of swine slaughterhouses. The samples were collected in a local abattoir and submitted to Liquid-Liquid Extraction (LLE) and Solid-phase Extraction (SPE) with XAD-4 resin as stationary phase. The instrumental analysis was performed by Gas Chromatography with Mass Spectrometer Detector (GC/MSD). The compounds present in the LLE and SPE extracts were identified by the GC/MSD library (Wiley). The results pointed out that SPE and LLE can extract practically the same classes of compounds at the same amounts. LLE works well for the extraction of polar organic compounds, with acidified samples, while SPE presents a better performance for the extraction of less polar organic compounds. Aldehydes were main class of the compounds extracted by SPE and LLE and decenal was the major aldehyde identified. Fatty alcohols and carboxylic acids were also identified but in minor proportions.
The resurgence of hydatid disease in communities in northern Israel led to epidemiologic studies in 1988 in the town of Yirka, a semirural Druze community of 8200 persons. In domestic animal surveys, 8% of 63 dogs tested after an arecoline purge and 10% of 255 sheep at the abattoir were found to be infected. In a randomized serosurvey of 758 persons using the indirect hemagglutination test (positive titer greater than or equal to 1:512), 9% (68) had a titer greater than or equal to 1:64, 2.2% had a titer greater than or equal to 1:256 and 0.9% had a titer greater than or equal to 1:512. Of those with titers greater than or equal to 1:64, 59 were evaluated by abdominal sonography and chest x-ray: 6 of the 59 were found to be persons who previously had had a liver or a lung hydatid cyst surgically removed; 6 other persons (5 with negative indirect hemagglutination titers) were found to have a newly detected asymptomatic cyst. Of the latter group, hydatid confirmation was subsequently obtained by surgery for 2 persons and by arc 5 and/or immunoblot assay for 4 persons. Thus, the cumulative percentage of confirmed present or recent past hydatid infections was 12/758 (1.6%) leading to an extrapolated rate of 1583/100,000 persons. This ranks Yirka among the highly endemic areas for hydatidosis worldwide. The study also showed that imaging methods were more sensitive than the indirect hemagglutination serologic test for conducting a prevalence survey.
The mode of transmission of Staphylococcus saprophyticus, a urinary tract pathogen, was investigated in three related studies. The presence of this organism was sought, during a period of 1 year, in 1331 specimens of various foods, in 920 beef and pork carcasses and on 107 cultures which had been inoculated directly from abattoir workers' protective gloves. Staphylococcus saprophyticus was found to contaminate 16.4% of the various food samples with a high prevalence of 34% in raw beef and pork. It was common in both domestic and imported raw meat products. There was no seasonal variation in the presence of S. saprophyticus in the samples obtained from carcasses. The bacterium was found in 69% of all cultures from the workers' protective gloves. We conclude that S. saprophyticus, originating from slaughtered animals, contaminates food and eventually colonizes the human intestinal tract.
Swab specimens from 4357 pig hindquarters provided for production of cured raw ham were contaminated with Staphylococcus aureus in 22.7%. The bacterial counts for S. aureus on the rind surface of the raw, uncured ham were between 10(1) and 10(3) cfu/cm2 in 89% of the positive samples. In the remaining 11% of contaminated ham counts of 10(3) to 10(6) cfu/cm2 were determined. There were major differences in the rate of contamination between pork from different suppliers. Questioning the suppliers revealed that pork highly contaminated with S. aureus could be traced back to certain abattoirs. This suggests that the technique of slaughter, concomitant hygiene precautions and the subsequent refrigeration of the carcasses affect the contamination of the meat with staphylococci.
Lactic acid decontamination (LAD) was carried out in an abattoir on pork carcasses, artificially contaminated with Salmonella typhimurium in faeces suspensions. The surface contamination with S. typhimurium ranged from 1-2 log10 cfu/cm2. Before cold and hot LAD was undertaken, the inoculum was allowed to adhere to the meat surface for 20 min. Cold LAD consisted of treatment for 60 s with 2% (pH 2.3) or 5% (pH 1.9) lactic acid (LA); for hot LAD the exposure times were 30, 60, 90 and 120 s. The spray nozzle temperatures were 11 degrees C and 55 degrees C, and that of the treated meat surface 16-18 degrees C and 36-38 degrees C, respectively. Treatment with cold 2% and 5% LA for 60 s eliminated S. typhimurium from pork carcasses inoculated with ca. 1 log10 cfu/cm2, but not from those inoculated at ca. 2 log10 cfu/cm2. However, this could be achieved by hot 2% and 5% LA sprayed for 60-120 s. Also exposures of at least 30 s using these hot LA solutions eliminated S. typhimurium consistently from carcasses inoculated with ca. 1 log10 cfu/cm2. Rinsing-off contributed only marginally to contamination reduction. Application of 2% or 5% LA for 120 s led to an unacceptable deterioration of the organoleptic qualities of the meat. Addition of nicotinic and ascorbic acid as colour stabilizers to the spraying solutions reduced these changes to just acceptable levels when 2% LA was used.
A grid was drawn on a lateral half of 10 hides, from cattle infected with both Onchocerca gutturosa and Onchocerca lienalis, which were obtained from an abattoir in central Florida. Paired 7-mm diameter skin cores were taken from 24 grid sites on each hide and microfilariae (mff) extracted to determine their distribution. The only consistent pattern distribution was that mff were recovered from all 10 hides at four sample sites along the ventral midline near the umbilicus. Microfilariae were also frequently found in large numbers along the dorsal cervical midline and in the region of the tarsal and carpal-metacarpal joints.
Hydatidosis was seen in 38.90% of cattle, 33.06% of buffaloes and 58.9% of camels slaughtered at a local abattoir. No statistically significant seasonal difference in prevalence was observed. Most cysts (63.14%) were infertile. Protein and carbohydrate contents of fluid from fertile and infertile cysts did not differ significantly. Sensitivity, specificity and efficiency of indirect haemagglutination test and enzyme-linked immunosorbent assay were low.
An abattoir survey on gastrointestinal parasites of the dromedary (Camelus dromedarius) was carried out in March/April 1987 in northern Mali. Parasitological examination of the digestive tract contents of 11 dromedaries revealed that Haemonchus longistipes was the predominant parasite with a prevalence as high as 85.7%. The genera Impalaia, Trichostrongylus, Cooperia, and Oesophagostomum were also recovered, but with a lower level of infection and frequency. The larval stage of the canine cestode Echinococcus granulosus was recorded in a single instance.
Trichinella spiralis infection has been reported sporadically in several areas in Central and South America. However, several countries, including Bolivia, have not reported trichinellosis in animals or humans. A small survey of pigs slaughtered in an abattoir in Bolivia was undertaken during September 1991, to determine the presence of Trichinella spiralis. In a group of 100 pigs slaughtered consecutively on a single day and tested using the pooled digestion method, two of eight pools (25%) were positive. Twenty-one of 188 pigs (11.2%) from ten communities slaughtered consecutively on a second day tested positive for the presence of antibodies to Trichinella spiralis using an enzyme-linked immunosorbent assay. It was concluded that trichinellosis is present in pigs in Bolivia and the rate of infection may be quite high.
From August 1991 to July 1992, a total of 48 abomasa from sheep and 48 from goats were examined at the Jeddah abattoir for the presence of adult and larval nematodes. Twenty-three of the sheep (47.9%) and 21 of the goats (43.8%) were infected with nematodes, a total of eight species being found. Haemonchus contortus and Marshallagia marshalli appeared to be the most important parasites. Other species were less prevalent: Trichostrongylus axei, Camelostrongylus mentulatus, Parabronema skrjabini, Ostertagia circumcincta, Skrjabinagia lyrata, Ostertagia trifurcata. Overall worm counts and infection rates were lowest in the winter. Larval inhibition plays an important role in the Marshallagia life cycle, since this parasite survives the dry season as arrested larvae. By contrast, inhibition of Haemonchus was less pronounced and the parasite survived the same season as adults and larvae. Although the phenomenon of hypobiosis was observed, the number of inhibited larvae was too small to be expected to induce clinical symptoms after resumption of development.
Gastrointestinal tracts of 73 indigenous sheep were obtained from the Medan abattoir in North Sumatra, Indonesia, and examined for the presence of helminths. A total of 13 species of helminths, eight nematodes, one cestode and four trematodes were encountered. All sheep were infected by more than one species of nematodes. Trichostrongylus colubriformis, T. axei and Haemonchus contortus were the most common species. The intensity of the nematode infections was very high: the average burden was over 7500 worms. Trichostrongylus spp. accounted, on average, for 81.5% of the total nematode burden. No influence of age on total nematode burden could be shown. Eurytrema pancreaticum and Schistosoma spindale were found in 23.3% and 4.1% of the examined sheep, respectively. The results are discussed in relation to common management and helminth control systems.
A sero-epidemiological study of Taenia saginata cysticercosis was carried out to determine the prevalence and distribution of the infection in three provinces of Kenya. Serum samples and meat inspection records were collected from cattle at slaughter at export and district abattoirs. Cattle origin and the presence of T. saginata cysticerci were noted as was the prevalence of other helminths such as Echinococcus granulosus and Fasciola gigantica. Serum samples were screened for circulating parasite antigen using a monoclonal antibody-based enzyme-linked immunosorbent assay (Ag-ELISA) and for ante-parasite antibody by indirect ELISA (Ab-ELISA). Eighty per cent of the sera were collected from cattle from the Rift Valley Province of Kenya. The prevalence of T. saginata cysticercosis and the other helminth infections varied between districts and was particularly high in Narok. Animal husbandry practices in arid areas such as Narok may be particularly conducive to transmission. The potential value of the Ag-ELISA for use in sero-epidemiological studies was verified by this study. It detected at least twice as many cases as T. saginata cysticercosis as meat inspection and, of the three methods investigated, was considered the most valuable.
During one year, 597 cattle were examined for Mammomonogamus laryngeus infections in the abattoir of Cagayan de Oro, Philippines. The overall prevalence was 23% with no obvious seasonal variations. The infection rate was 19% in animals up to 4 years old and 24% in animals 4-12 years old. In infected animals up to 8 years old an average of eight parasite pairs was found; infected animals of 8-12 years old had 5.2 parasite pairs on average. All worms were located on the posterior side of the epiglottis. Field observations on cattle in Mindanao revealed that adult M. laryngeus had developed on the epiglottis of a tracer calf that had been on pasture for 30 days and then kept away from possible nematode infection for 28 days.
Twenty-eight strains of Pasteurella multocida were isolated from 12 Danish and two Canadian abattoir pigs. Fourteen strains were isolated from pulmonary inflammatory lesions, and 14 strains were isolated from kidneys of the same animals. Phenotypical and genotypical characteristics of the strains were evaluated with a view to determine if P. multocida isolated from kidneys might have been disseminated from the lungs. All field strains were capsular type A. The biochemical reactivity in the API-20E and API-ZYM commercial test-kits was uniform with the exception of alpha-glucosidase activity which was present at low levels in only ten of the strains. One strain was markedly serum sensitive, six strains slightly sensitive and the remaining were serum resistant. The peptide patterns obtained by polyacrylamide gel electrophoresis of whole cell proteins of the strains were very uniform with the exception of differences in intensity of bands in the 38 and 34 kD regions. The pattern of oligonucleotides obtained after electrophoresis of total genomic DNA digested with BamHI showed that the paired isolates had identical patterns in eight of the 14 animals. It is therefore likely that isolates from kidney lesions represent blood borne dissemination from primary pulmonary lesions.
Kidneys and matched serum samples were obtained from 368 pigs slaughtered at three Victorian abattoirs, and originating from 42 farms. Macroscopic lesions (white spots) were observed on 102 of the kidneys. Serum samples were tested by the microscopic agglutination test (MAT) and by an IgM enzyme immunoassay (EIA). Kidneys were cultured for leptospires, examined histologically after Warthin-Starry silver staining and after immunogold silver staining (IGSS), and tested for leptospiral DNA by DNA hybridization. Forty-four infected pigs were identified by culture or immunogold silver staining of kidneys or by high MAT titres (greater than or equal to 1024). Infection was demonstrated in 7.5% of visibly normal kidneys, in 23.5% of kidneys with white spots, and in 48% of kidneys with large white spots, of 1 cm diameter or greater. The apparent (maximum) sensitivities of diagnostic procedures for detecting infection were as follows: MAT (at a titre of either 64 or 1024) 95%; IgM EIA 82%; culture 61%; presence of white spots 55%; IGSS 52%; presence of large white spots 30%; Warthin-Starry silver staining 20%. IGSS, Warthin-Starry staining and DNA hybridization all appeared to be highly specific. Of 22 kidney sections identified as positive by IGSS, 13 showed intact leptospires, and these kidneys were all culture-positive. Nine others showed leptospiral antigen in the kidney tubules but no intact leptospires. Only five of these kidneys were culture-positive.
A tentative diagnosis of bovine tuberculosis can be made following the macroscopic detection at necropsy of typical lesions. Histo-pathological examination of the lesion may increase the confidence of the diagnosis but bacteriological isolation of Mycobacterium bovis from the lesion is the only way to make a definitive diagnosis. The sensitivity of gross post mortem examination is affected by the method employed and the anatomical sites examined. Careful examination of as few as 6 pairs of lymph nodes, the lungs and the mesenteric lymph nodes can result in 95% of cattle with macroscopic lesions being identified. Although during post mortem inspection of carcases at abattoir all the principle sites where lesions are to be found were examined, this procedure was found to be insensitive for the detection of lesions. To determine the significance of cattle that give a positive reaction in diagnostic tests but do not have visible lesions (NVL), a bacteriological examination is necessary. NVLs may be due to early infection, poor necropsy technique or infection with mycobacteria other than M. bovis. M. bovis was found to survive best in frozen tissue and the tissue preservative, sodium tetraborate, was found to have adverse effects on viability. It was found desirable to use two different culture media for the primary isolation of M. bovis; agar media for rapid growth and egg media for control of contamination. Additional control of contamination was achieved without adversely affecting the viability by treating the specimen before culture with 0.075% hexadecylpyridinium chloride. The addition of CO2 to the incubation atmosphere did not enhance the recovery of M. bovis. Conventional identification of isolates of M. bovis is by biochemical tests and cultural characteristics, but methods employing monoclonal antibodies and DNA probes may be used to obtain a rapid identification.
Sera from 98 abattoir workers were tested for IgG as well as for IgA to Campylobacter pylori, C. jejuni and Klebsiella. Clerical workers had significantly lower C. pylori and C. jejuni antibody levels than any of the groups in direct contact with freshly cut animal parts. No difference was found for antibodies to Klebsiella. Twenty-nine non-clerical workers with high IgG antibody levels against C. pylori consented to upper gastrointestinal endoscopy. C. pylori associated gastritis was found in all 29, and 4 weeks of colloidal bismuth subcitrate (240 mg/twice daily) was prescribed. On repeat testing at 3 months, all showed a decrease in IgG antibody levels to C. pylori but not to C. jejuni, whereas 18 untreated non-endoscoped workers showed no change. These findings raise the possibility that C. pylori infection is a zoonosis.