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At least 685 records · Page 38Linked to original sources
PubMed2004

Calcifying epithelial odontogenic (Pindborg) tumor. A clinical case.

Calcifying epithelial odontogenic tumor (CEOT), Pindborg tumor, is a rare benign odontogenic neoplasm representing about 0.4-3% of all odontogenic tumors. This tumor more frequently affects adults in an age range of 20-60 years, with a peak of incidence between 40 and 60 years. About 190 cases of CEOT have been reported in the dental literature. Fifty-two percent of cases of CEOT is associated with a tooth impacted and/or displaced by the tumor. The primary CEOT has a recurrence rate of 10-15%, after total excision, and its malignant transformation is a very rare occurrence. The authors report a case of primary intra-osseous CEOT, embedding the mandibular right second molar, in a 24 year-old male. Radiographs showed a well-defined unilocular osteolytic lesion, swelling and reabsorbing the mandible and displacing the inferior alveolar nerve. It was possible to perform conservative surgical treatment consisting of the enucleation of the tumor together with a portion of tumor-free bone cavity margin and the debridement of the inferior alveolar neuro-vascular bundle, which was surrounded by a tumor capsule-like structure. The postoperative histological examination of the tumor revealed typical benign features. The differential diagnosis and work-up of the tumor treatment are discussed in relation with its histological typing and localization in the jaws.

Adult↗
PubMed2004

Cloning and characteristics of an allene oxide synthase gene (TaAOS) of winter wheat.

Allene oxide synthase (AOS) is the first enzyme in the lipoxygenase pathway which leads to the formation of jasmonic acid (JA). A full length cDNA of TaAOS was cloned in winter wheat (Triticum aestivum L. cv. Jinghua No.3) seedlings. The open reading frame encompassed 1410 bp encoding a polypeptide of 470 amino acids with calculated molecular mass of 51.9 kD. Southern blot analysis suggested there are three copies of the gene in wheat genome. The TaAOS mRNA could be strongly induced by exogenous JA, and the highest level JA was observed after a 10 h induction. In situ RNA hybridization of seedling indicated preferential gene expression in young leaves, especially in the parenchyma cells around the vascular bundles, and the hybridization also showed that exogenous La(3+) could not suppress the expression of TaAOS induced by JA.

Amino Acid Sequence↗
PubMed2004

[Histological and morphological studies on the rhizomes of Curcuma].

OBJECTIVE: To provide some new evidences for the identification of medicinal materials of Curcuma. METHOD: Microscopic observation was made to characterize the rhizomes of Curcuma. RESULT AND CONCLUSION: There were no obvious histological and morphological differences among the rhizomes of Curcuma. The distribution of oil cells and vascular bundles as well as the number and diameter of xylem vessels were considered to be the distinguishing features of their rhizomes.

China↗
PubMed2005

[Distribution of Rubisco and RCA in Brassica chinensis chloroplasts and effect of TuMV-infection on their cellular localization].

The cellular localizations of Rubisco and Rubisco activase (RCA) in Chinese cabbage (Brassica chinensis L. cv. Suzhou) leaves were investigated by immunogold-labeling electron microscopy. The results showed that Rubisco and RCA were mainly located in chloroplasts of mesophyll, guard cell of stomatal apparatus and parenchyma of vascular bundle. A high density of gold particles were localized preferentially to the chloroplast stroma. In contrast, there were no specific binding of gold particles detected in the cytoplasm, vacuole, mitochondria and other organelles. As infected by turnip mosaic virus, the density of gold particles decreased lightly in the abnormal chloroplasts and dropped by much lower (58.44% and 64.67% as that in the health chloroplasts respectively) in swollen chloroplasts. This indicated that virus infection caused the decreases of Rubisco and RCA in host plant, which then affected plant photosynthesis.

Brassica↗
PubMed2004

[Leaf anatomy of four species of Solanum L. section Acanthophora Dunal in Venezuela].

Anatomical characteristics of S. acerifolium Dun., S. agrarium Sendtn., S. capsicoides All. and S. mammosum L., leaves were studied in order to recognize some useful features for its interspecific delimitation. 5 to 10 healthy fully expanded leaves, taken from the middle third of the plant, were examined in at least 3 different plants for each studied species. Samples were preserved in 70% FAA. Additionally, herbarium specimens (4-5 leaves/specimen) were hydrated in lactophenol and examined. The middle third of the foliar lamina and the petiole were prepared following the classical techniques used for anatomical studies. Clearing process of the foliar lamina was also made. The results show similar histological configuration in all studied species. Among the most remarkable features are: the type of leaf regarding to stomata location, number of petiole layers, angular collenchyma, petiole cross section shape and the type of vascular bundles. Finally, it is concluded that the anatomical study of the leaves of mentioned species is useful for its delimitation.

Plant Leaves↗
PubMed2005

[Location and relative quantity of coumarins in the stem of Dendrobium thyrsiflorum].

AIM: To determine the location and relative quantity of coumarins in the stem of Dendrobium thyrsiflorum Rchb. f. , and to provide a scientific basis for evaluating and utilizing the famous medicinal plant. METHODS: The stems of one, two and three years old, separately, were collected in February. Location and relative quantity of coumarins in the top, middle and basal parts of each stem sample were determined by using laser scanning confocal microscopy (LSCM). ANOVA and Tukey's test were employed in the statistical analysis. RESULTS: The coumarins located mainly in vascular bundles, especially on the walls of the outer fiber cells. There was significant difference or great significant difference exist in every part of the stem from different ages. The values of significant difference were as follows: basal part P = 0.004 (< 0.01); middle part P = 0.009 (< 0.01); top part P = 0.036( < 0.05). CONCLUSION: Two years old stem could be the best choice when collecting Dendrobium thyrsiflorum in Feburary.

Coumarins↗
PubMed2005

[The dynamics of calcium distribution in stigma and style of lettuce (Lactuca sativa L.) before and after pollination].

Potassium antimonite was used to deposit calcium in the stigma and style of lettuce (Lactuca sativa L.) before and after pollination. The stigma of lettuce is two splits. Abundant calcium granules are displayed in the wall of papillae on the receptive surface of stigma before and after pollination, which may facilitate pollen germination. However, a few calcium granules in the wall of epidermis cell on no-receptive surface. Calcium distribution in style presents a gradient in transmitting tissue and parenchyma cells from the top to the base of the style before pollination. After pollination, calcium in transmitting tissue distinctly increased and its gradient distribution became more evident. Pollen tubes grow in the intercellular gaps of transmitting tissue. When pollen tubes grew into transmitting tissue, calcium granules in parenchyma around transmitting tissue decreased, suggesting a calcium movement was controlled by pollen tubes. The calcium gradient distribution also appeared in the trachea of vascular bundle of style. In general, calcium in style displays a feature of time-special distribution: transmitting tissue doesn't need much more calcium that is only stored in the parenchyma before pollination. However, calcium in parenchyma cells may be transported to transmitting tissue and make the latter contain more calcium to form an evident calcium gradient and meet the requirement of pollen tubes directionally growing after pollination. This is the second sample of calcium gradient existing in style, which was found by using potassium antimonite method.

Calcium↗
PubMed2005

[The distribution and viability changes of the Agrobacteria in the vacuum infiltrated plants of Chinese cabbage].

To understand further the mechanism of agrobacteria transformation by vacuum infiltration, the distribution and viability changes of the agrobacteria in infiltrated Chinese cabbage( B. rapa ssp. chinesis) plants were investigated by histochemical staining and bacteria plating techniques. Results revealed that there were different quantities of Agrobacteria in different organs, among which the most quantity occurred in flowers, especially in ovules. The Agrobacteria lied in the space between cells and concentrated in the vascular bundle no matter the type of the organs examined. The CFUs (colony forming unit) of the Agrobacteria derived from the flowers were always the most in every experiment. The viabilities of the agrobacteria in the plants declined dramatically along with the elongation of the time after plant infiltration in all organs, but there were still 10(3) CFUs per gram fresh tissue in the flowers after 15 days of infiltration. These results may explain the fact that the ovules were the targets for the Agrobacteria mediated vacuum infiltration transformation.

Brassica↗
PubMed2005

[Pharmacognostic studies on mangrove Acanthus ilicifolius].

OBJECTIVE: In order to prove a basis for rational exploitation and utilization of the herb, the charaeteristic feature for Acanthus iliciflius was studied. METHOD: The botanical and pharmacognostic features of the collected original plant were stadied. RESULT: A. ilicifolius showed obvious pharmacognostic characters. The aerate cells of the root were upgrowth. and four vascular bundles were found in leaf venation. CONCLUSION: The distinct characteristic features revealed in this studies can serve as evidence for the identification of A. ilicifolius.

Acanthaceae↗
PubMed2005

Function analysis of promoter trapping system after inserted into cotton (Gossypium hirsutum L. ) genome.

The technique of promoter trapping was developed to investigate its viability in cotton ( Gossypium hirsutum L.) functional genomics. 141 independent transformants of cotton were generated via Agrobacterium tumefaciens mediated transformation, of which 97% showed positive by PCR detection. The reporter, GUS gene, was expressed to different extent in different organs, with a frequency of 48% in roots, 9.2% in vascular bundles of stem, 5.2% in leaves, and 51% in flowers. Meanwhile, we discovered that there existed great differences in expression patterns among different transgenic lines. Their GUS expression patterns were organ- or tissue-specific or ubiquitous in all of the plants. The promoter trapping system developed here was characterized as an effective method for creating mutants with diverse reporter gene expression patterns, which laid a solid foundation for further research of functional genomics in cotton.

Base Sequence↗
PubMed2006

[Expression of an Arabidopsis actin-depolymerizing factor 4 gene (AtADF4) in tobacco causes morphological change of plants].

The actin-depolymerizing factor 4 gene (ADF4) of Arabidopsis thaliana was cloned and sequenced (Figs.1, 2). The plant expression vector with ADF4 was constructed and transformed into tobacco by Agrobacterium tumefaciens. Molecular identification showed that the ADF4 gene was integrated into the genome of tobacco and expressed in transgenic tobaccos assayed by PCR and RT-PCR (Fig.3). Expression of an Arabidopsis thaliana ADF4 gene in tobacco resulted in morphological change of plants. The effects of ADF4 on transgenic tobaccos growth were as follows: the hypocotyls of transgenic plants were wavy, especially in darkness, whereas those of the control were straight (Fig.4A); the root hairs of transgenic plants were less than the control, and they were also wavy (Fig.4B); the parenchyma cells of transgenic plants were larger than the control and the arrangement of vascular bundle was out of order (Fig.4C); the flowering time of T(2) line was at least 7 days later than the control; the pollen tubes of transgenic plants were shorter than those of the control (Fig.4D).

Agrobacterium tumefaciens↗
PubMed2005

[Morphology of seed germination and parasitism in Cistanche tubulosa].

OBJECTIVE: To understand the process of Cistanche tubulosa. METHOD: The process of seed germination and parasitism was observed using stereomicroscope. RESULT: Seedling of C. tubulosa sprouted after forty day without host root's contact in fields, a tube-like-organ formed and grew auger-type from host root, the tuber apex where touches host root swelled and formed haustorium. Haustorium intruded host root epidermis and vascular bundles, and released brown substances. Then, embryo bud with six or more young leaves formed, finally the swelled tuber-like-organ broken and seed coat shed. Due to the parasitism of C. tubulosa, the host root near stem site swelled, but the other part, shrunk and disappered gradually. CONCLUSION: Seed of C. tubulosa could germinate indepently in fields. Tuber-like-organ formatin, haustorium formation and bud formation are key steps of C. tubulosa seedling development.

Cistanche↗
PubMed2006

[The localization of arabinogalactan-proteins in stigma and style of Nicotiana tabacum L].

The distribution patterns of arabinogalactan-proteins (AGPs) in stigma and style of Nicotiana tabacum L. were studied with western blotting, immunohistochemistry and ultracytochemistry techniques. The results showed abundant AGPs accumulated in the stigmas and styles (Fig.1, Plate I). AGPs were mainly distributed in the cytoplasm of epidermal cells and extracellular matrix of the secretory zone of stigma (Plate II) and no significant changes of the distribution of AGPs in stigma were detected before and after pollination. In styles, AGPs were mainly distributed in the outer cell walls of the epidermal cells, the cytoplasm of peripheral cells of vascular bundles and the extracellular matrix of the transmitting tissues (Plate III). As the pollen tube tips penetrated through, the accumulations of AGPs in the extracellular matrix of the stylar transmitting tissues decreased, but abundant AGPs were founded in the cytoplasm and cell walls of the passing pollen tubes (Plate III).

Blotting, Western↗
PubMed2006

[Ultrastructural observation related to cell-to-cell movement and long-distance systemic transport on the hosts infected with BBWV 2].

The alteration of ultrastructure in Pisum sativum and Vicia faba leaf cells infected with B935 isolate of BBWV 2 were investigated by electron microscopy, immunogold-labeling technique. The results showed that the membranous proliferation, virus-formed crystals and tubular structures were found in leaf cells of two hosts. At early stages of infection, the tubules containing virus-like particles associate with plasmodesmata in mesophyll cell. Immunogold particles anti-BBWV 2 were localized to the plasmodesmata modified by tubules passing through them. The membranous proliferation and virus-formed tubules were also found in the parenchyma cells, companion cells and transfer cells of vascular bundle. Some virus-like particles located within sieve tube can be labeled immunogold particles anti-BBWV 2. These suggest that BBWV 2, similar CPMV, produce tubules extending into the plasmodesmata. Virions assembled in the cytoplasm are escorted to the tubular structures through interactions with their MP and are then transported to the adjacent cell. Many 160 nm in diameter virus-formed tubules in the cytoplasm, as a special aggregate, not directly relate to cell-to-cell movement; Intact virions are long-distance sustemic transported possibly through sieve elements.

Biological Transport↗
PubMed2006

[The features of distribution of polysaccharide and lipid in the developing anther of Lycium barbarum L].

Polysaccharide and lipid in the anthers of Lycium barbarurn L. at different stages were examined with cytochemical techniques. At the stage of sporogenous cell, many starches have been storied in parenchyma around vascular bundle, epidermis and endothecium cells but no starches in sporogenous cells, tapetal and middle layer cells. At the stage of tetrad, there were many starches still in epidermis and endothecium, however tapetal cells began to accumulate lipid drops, suggesting that tapetal cells transformed polysaccharides into lipid. Tapetum degenerated at the late stage of microspore and the lipid drops moved into locule. During microspore development neither starches nor lipid drops were accumulated in the cell. After the division of microspore, some lipids drops appeared in 2-cellular pollen, and then some starches also appeared in the pollen. Two days before anthesis, there were many lipid drops and starches located in nearly mature pollen grains, suggesting that pollen of Lycium barbarurn L. has a function of transforming lipid into polysaccharide. The temporal and spatial features of polysaccharide and lipid material accumulated and distributed in anther during its development reflect the transformation of physiological function of the cells consisting of anther. This research will help us to understand the mechanism of anther development.

Flowers↗
PubMed2006

[Combined scapular/parascapular bilobar flaps for reconstruction of severe neck contracture].

OBJECTIVE: To discuss the reconstruction of severe neck contracture by transplanting combined scapular/parascapular bilobar flaps, and the probability to reestablish three-dimensional movement of the neck. METHODS: From January 2003 to November 2004, 9 cases of sustained severe neck contractures were treated (aged 9-32 years). The combined scapular/parascapular bilobar flaps, pedicled on the circumflex scapular vascular bundle, were microsurgically used to cover the soft tissue defect after excision of hypertrophic scar and release of contracture. The maximum size of the combined bilobar flap was 20 cm x 8 cm to 20 cm x 11 cm, while the minimum one was 15 cm x 4 cm to 15 cm x 6 cm. RESULTS: The combined scapular/parascapular flaps were successfully used to treat 9 cases of severe neck contracture. All patients were satisfied with the final functional and aesthetic results. There was no recurrence during 3-9 months follow-up for 8 patients. The cervicomental angle was 90-105 degrees. CONCLUSION: The combined bilobar scapular/parascapular flap, providing a large area of tissue for coverage in three dimensions with a reliable blood supply by only one pedicle anastomosis during operation, is a good option for reconstruction of the severe neck contracture.

Adolescent↗
PubMed2006

An investigation of cellular distribution of manganese in hyperaccumlator plant Phytolacca acinosa Roxb. using SRXRF analysis.

Phytolacca acinosa Roxb. (P. acinosa) is a recently discovered manganese hyperaccumulator plant from southern China. It is a good candidate for phytoremediation of manganese(Mn) polluted soil for its high biomass and fast growth. Knowledge of the tissue localization and identification of heavy metals can provide essential information on metal toxicity and bioaccumulation mechanisms. Synchrotron radiation X-ray fluorescence spectroscopy (SRXRF) microprobe was used in this study to investigate the cellular distributions of Mn and other elements in root, stem, leaf, petiole and midrib of P. acinosa. The highest Mn content was found in the vascular tissues of root, stem, petiole and midrib. Cortex in root played a key role in Mn absorption and Mn was limited in the vascular bundle during the process of transportation in stem. Moreover, Mn content in leaf epidermis was higher than that in mesophyll, which suggested that the sequestration of Mn in leaf epidermis might be one of the detoxification mechanisms of P. acinosa. The significance of other elemental (such as P, S, K, Ca, Fe, Zn and Cu) distribution patterns and the correlation with Mn were also discussed.

Biodegradation, Environmental↗
PubMed1991

[Experimental study of the treatment of avascular osteonecrosis].

The results of treating avascular necrosis of the femoral head of 32 mongrel dogs with 4 different methods were compared. The treating methods differed only in the type of tissues transplanted into the femoral head specimens: (1) with blood vessel and muscle pedicled bone graft, (2) with muscle pedicled bone graft, (3) with vascular bundle transplant and (4) with free bone-piece graft. The results showed that graft of type 1 or 2 or 3 transplantation brought about new bone formation and dead bone replacement in different degrees and varying activity, obviously more potent in those with type 1 and 2 than those with type 3. While those with type 4 showed little living activity and the grafted bone-piece remained dead. These differences may be based on the facts that transplants type 1 & 2 carry with them living tissues, active bone-forming cells and many other bone growth stuffs and factors necessary for conduction and induction for new bone formation, in addition to blood supply. While type 4 transplant can meet nutritional needs of metabolism and tissue growth only. Accordingly, transplantation of type 1 or 2 graft could be a preferable choice of treating clinical cases with femoral head necrosis.

Animals↗