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At least 685 records · Page 38Linked to original sources
PubMed1986

Investigation on the occurrence of soluble lectins in mammalian nervous tissue extracts.

Five brain or retina crude extracts obtained from adult mammalians and nine fractions of brain extracts prepared by chromatography were screened for their lectin activities. All crude extracts and several fractions contained agglutinins reacting with neuraminidase-treated rabbit red blood cells. Hemagglutination activity varied widely with the method of preparation of the extracts. Hemagglutination inhibition tests were carried out to look for possible differences in the specificities of the agglutinins. All were found to be D-galactosyl specific. Each crude extract was found to contain a second lectin activity, which was detected using ethanol-treated rabbit erythrocytes known to react with heparin-binding lectins. Hemagglutination and inhibition studies showed that they completely differ from the galactoside-binding lectins detected previously. The possible functions of these lectins are discussed.

Animals↗
PubMed2005

Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results: metabolomic analysis of mouse tissue extracts.

The potential for the utilization of GC x GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach.

Animals↗
PubMed2006

Identification and quantification of GC-rich oligodeoxynucleotides in tissue extracts by capillary gel electrophoresis.

Capillary gel electrophoresis (CGE) is a widely used method for quantification of oligonucleotide-based drugs, such as CpG oligodeoxynucleotides (CpG ODN), aptamers and small interfering ribonucleic acids (siRNAs) that allows accurate quantification of parent compound as well as metabolites. Stable secondary structure formation of these molecules frequently prevents analysis by conventional CGE methods and impedes pharmacokinetic assessment. Herein, we describe development of a CGE method for identification and quantification of complex mixtures of secondary structure forming GC-rich ODN in biological samples at dose levels of 0.5mg/kg and above. Samples containing GC-rich CpG ODN and metabolite markers were treated by solid-phase-extraction (SPE) and subsequently analyzed by CGE using a 50cm neutrally coated capillary at 60 degrees C together with a 7M urea buffer system containing 30% dimethylsulfoxide (DMSO). Peak resolutions >or=1 were typically achieved, enabling pharmacokinetic assessment of secondary structure forming oligonucleotides in biological samples that hitherto were unsusceptible to quantitative analysis.

Animals↗
PubMed2000

Nutritional and somatotropin regulation of the mitogenic response of mammary cells to mammary tissue extracts.

Our objective was to investigate the mitogenic response of primary mammary epithelial cells to extracts of mammary parenchyma from 24 prepubertal Friesian heifers treated with placebo or growth hormone at either a low or a high feeding level. The mitogenic responses to mammary extracts were tested by using primary mammary epithelial organoids obtained from prepubertal heifers cultured for 4 to 5 d in collagen gels in serum-free medium supplemented to 5% concentration of the mammary extracts. Cell proliferation was determined using [methyl-3H]thymidine incorporation as a measure of DNA synthesis. High feeding level reduced DNA synthesis in response to mammary extracts. At low feeding level, growth hormone treatment decreased DNA synthesis in response to mammary extracts whereas, at high feeding level, growth hormone increased DNA synthesis in response to mammary extracts. These results suggest that locally produced growth factors are involved in the regulation of mammary development when mammary growth is modulated by feeding level and growth hormone treatment.

Animal Feed↗