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Performance of semiquantitative food frequency questionnaires in international comparisons. Mexico City versus San Antonio, Texas.

International dietary comparisons present a number of methodologic difficulties. We developed two semiquantitative food frequency questionnaires for use in Mexico City and San Antonio, Texas. The Mexico City questionnaire contained 85 food items and the San Antonio questionnaire contained 108. Thirty-six items were common to both questionnaires. The questionnaires were administered to 128 Mexican Americans, 35 to 64 years old, residing in a low-income barrio neighborhood of San Antonio and to 644 similarly aged Mexicans residing in a low-income "colonia" of Mexico City. Major differences in fat and carbohydrate intake as a percent of total kilocalories were observed between the two study groups, with Mexico City residents consuming approximately 18 to 21% of calories from fat and 68 to 72% from carbohydrate compared to 29 to 33% from fat and 48 to 52% from carbohydrate for Mexican Americans from San Antonio. Both of these differences were highly statistically significant (P < 0.001). A number of arguments support the validity of these findings. First, the distribution of high- and low-fat foods was similar on both questionnaires. Also, of the top-ten foods (as contributors to total kilocalorie intake) in San Antonio, seven were high in fat (> 30% of kilocalories), whereas this was true of only three of the top-ten foods in Mexico City.

Adult↗

Food-frequency questionnaire validation among Mexican-Americans: Starr County, Texas.

A food-frequency questionnaire (FFQ) for low-income Mexican-Americans in Starr County, Texas, was developed as part of an epidemiologic study of gallbladder disease during 1985 and 1986. The FFQ was developed from 7-day food records collected from the first sample. In the validity study, using the second sample, correlations between nutrients calculated from 3-day food records and the FFQ were 0.77, 0.76, and 0.61 for energy, total fat, and saturated fat, respectively. In the reliability study, using the third sample, for the 1-month interval between baseline and a repeat FFQ measurement correlations ranged from 0.90 for energy to 0.85 for total fat and for the 2-month interval they were 0.84 for energy and 0.70 for total fat. The high correlations are largely explained by the lack of diversity in the diets of Starr County individuals which facilitated the high agreement between the FFQ and the food records for estimates of energy, fats, and cholesterol.

Adult↗

Statistical investigation of polychlorinated dibenzo-p-dioxins and dibenzofurans in the ambient air of Houston, Texas.

Polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (dioxins and furans) are persistent, bioaccumulative and highly toxic chemical constituents that appear in the environment at very low and difficult to measure levels. Although dioxins and furans are widely recognized as toxic contaminants needing regulation, their temporal and spatial concentration profiles and the factors impacting their partitioning and congener distribution are only moderately understood. This paper presents the results from one of the most extensive ambient air dioxin and furan sampling efforts conducted in a geographic area to date. The data consist of monthly ambient concentrations of 17 of the most toxic congeners of dioxins and furans collected at five locations in Houston, Texas over a calendar year. The results showed a total annual mean dioxin and furan congener concentration of 1047 fg/m3 and an annual mean summation operatorI-TEQ concentration of 15 fg I-TEQ/m3 (16 fg WHO-TEQ/m3). These results, when compared with proximate data from the USEPA National Dioxin Ambient Monitoring Network (NDAMN), revealed significant (at the P0.05 level) differences between Houston and the NDAMN sites, suggesting the existence of local dioxin sources. A temporal analysis of the data showed that the congener concentration distribution varies over time, depending on the level of chlorination, type of congener, and temperature. The results also suggested that the fluctuations of congener concentrations and I-TEQ concentrations are closely correlated with minimum relative humidity, mean temperature, and mean NOx concentration.

Air Pollutants↗

Trace metal partitioning in Thalassia testudinum and sediments in the Lower Laguna Madre, Texas.

Seagrass communities dominate the Laguna Madre, which accounts for 25% of the coastal region of Texas. Seagrasses are essential to the health of the Laguna Madre (LM) and have experienced an overall decline in coverage in the Lower Laguna Madre (LLM) since 1967. Little is known on the existing environmental status of the LLM. This study focuses on the trace metal chemistry of four micronutrient metals, Fe, Mn, Cu, and Zn, and two non-essential metals, Pb and As, in the globally important seagrass Thalassia testudinum. Seasonal trends show that concentrations of most essential trace metals increase in the tissue during the summer months. With the exception of (1) Cu in the vertical shoot and root, and (2) Mn in the roots, no significant positive correlation exists between the rhizosphere sediment and T. testudinum tissue. Iron indicates a negative correlation between the morphological units and the rhizosphere sediments. No other significant relationship was found between the sediments and the T. testudinum tissue. Mn was enriched up to 10-fold in the leaf tissue relative to the other morphological units and also enriched relative to the rhizosphere sediments. Both Cu and Mn appear to be enriched in leaf tissue compared to other morphological units and also enriched relative to the Cu and Mn in the rhizoshpere sediments. Sediments cores taken in barren areas were slightly elevated in Zn relative to the rhizosphere sediments, whereas no other metals showed statistical differences between barren sediment cores and rhizosphere sediments. However, no correlation was measured in T. testudinum tissue and Zn in rhizosphere sediments. Previous studies suggested that Fe/Mn ratios could indicate differences between seagrass environments. Our results indicate that there is an influence from the Rio Grande in the Fe/Mn signature in sediments, and that ratio is not reflected in the T. testudinum tissue. The results from this study show that the LLM contains trace metal concentrations less than or equal to values for uncontaminated locations worldwide. In addition, there appears to be a complex partitioning in the trace metals in the morphological units of T. testudinum tissue and that analysis only of the leaf may not be indicative of the trace metal levels in this important seagrass species.

Environmental Monitoring↗

Questions regarding environmental mercury release, special education rates, and autism disorder: an ecological study of Texas by Palmer et al.

Palmer et al. present an analysis in which they look for an association between Toxic Release Inventory (TRI) reporting data for mercury and rates of autism and special education enrollment in Texas. In their analysis, the link between TRI release data and actual mercury exposure is not clear at all, and thus the conclusions drawn from the analysis are questionable.

Autistic Disorder↗

Dietary intakes at child-care centers in central Texas fail to meet Food Guide Pyramid recommendations.

The purpose of this study was to compare the dietary intakes of children who attend child-care centers with the recommendations of the Food Guide Pyramid for Young Children. Three-day dietary records were obtained for 50 children ages 3 to 5 years old who attended nine child-care centers in central Texas. Dietary intakes during child care were measured by a researcher, and dietary intakes at home were recorded by a parent. During child care, the 3-year-old children consumed sufficient fruits and meat/alternates, but not sufficient grains, vegetables, or dairy to meet two thirds of Food Guide Pyramid for Young Children recommendations. The 4- and 5-year-old children only consumed sufficient dairy. Ninety-one percent (20 of 22) of the 3-year-old children met two thirds of their estimated energy requirements during child care, compared with 5% (1 of 20) of 4-year-old children and 25% (2 of 8) of 5-year-old children. Intakes at home did not compensate for lack of grain and vegetable consumption during child care.

Child Day Care Centers↗

Factors influencing implementation of the Coordinated Approach to Child Health (CATCH) Eat Smart School Nutrition Program in Texas.

The purpose of this research was to evaluate factors influencing the implementation of the Coordinated Approach to Child Health (CATCH) Eat Smart School Nutrition Program in Texas using data from the CATCH dissemination study. A mail survey was sent to school foodservice personnel (N = 213) who attended a CATCH training from August 2000 through January 2002. A response rate of 40% (n = 85) was achieved. The mean score for the percentage of CATCH Eat Smart guidelines implemented was 80.44. Multivariate linear regression analysis revealed that, after adjusting for age and number of years employed in school foodservice, the following factors were significantly associated with the percentage of CATCH Eat Smart guidelines implemented: utility of CATCH and CATCH Eat Smart in meeting requirements for Coordinated School Health Programs (P = 0.006), school foodservice personnel's satisfaction with food made using the CATCH Eat Smart guidelines (P = 0.008), utility of CATCH in facilitating interschool communication about children's health (P = 0.019), and perceived student satisfaction with food made using the CATCH Eat Smart guidelines (P = 0.046). These results suggest that dissemination approaches for Coordinated School Health Programs should focus on ways to enhance program satisfaction, be consistent with legislated mandates, and increase interschool staff communication to increase program implementation by school foodservice personnel.

Adolescent↗

The thyroid gland in bottlenose dolphins (Tursiops truncatus) from the Texas coast of the Gulf of Mexico: normal structure and pathological changes.

Fresh thyroid glands (n=60) from Atlantic bottlenose dolphins that died after stranding along the Texas coast between 1991 and 2005 were examined. Organ weight ranged from 11 g in a neonate (length 109 cm) to 58 g in a large (249 cm) male. More typical weights were 25-45 g (mean=30.6 g). Glands tended to be larger in pregnant and lactating females (mean 37.4 g; n=5) than in non-pregnant animals of comparable size. In infancy, the gland tended to be compact, relatively homogeneous, and sometimes partly lobular, but with advancing age it became more lobular, the lobules being defined by fibrous bands. In one 8-year-old female (233 cm), and in a large male (295 cm) aged>25 years the gland was represented by a cluster of lobules. Lobulation was not necessarily accompanied by increased weight, distinguishing it from hyperplasia. With age, variation in follicle size and colloid density tended to increase. Two animals (3%) had adenomas and five (8%) had discrete hyperplastic nodules, not to be confused with lobulation. Five (8%) had macroscopically identifiable colloid-filled cysts (1-4 mm in diameter). Nine animals (15%) had squamous cysts (4-15 mm) containing creamy white fluid. Other abnormalities included patchy or diffuse interstitial fibrosis (six cases, 10%) amyloidosis (two cases), thyroiditis (one case) and vasculitis (one case). No malignant neoplasms were found. Cells presumed to be C cells (light cells, parafollicular cells) were identified immunohistochemically with synaptophysin antibody.

Adenoma↗

Assessment of manure phosphorus export through turfgrass sod production in Erath County, Texas.

A best management practice (BMP) for exporting manure phosphorus (P) in turfgrass sod from the North Bosque River (NBR) watershed in central Texas was assessed using a geographic information system (GIS). The NBR watershed has a mandate to reduce the total annual P load to the NBR by 50% as a result of total maximum daily load regulation. Since dairy waste applications to fields are identified as the major nonpoint source of P to the river, innovative BMPs, such as export of manure P in turfgrass, will be needed to achieve the 50% reduction. However, methods are needed to evaluate the feasibility of these innovative management practices prior to their implementation. A geospatial database of suitable turfgrass production sites was developed for Erath County using GIS. Erath County largely encompasses the upper portion of the NBR watershed. Information from field experiments, production practices, and ground-truthing was used to search, analyze, and verify a geospatial database developed from national and regional sources. The integration and analyses of large databases supports the search by turf producers for sites suitable for turfgrass sod production in Erath County. In addition, GIS enables researchers and regulators to estimate manure P exports and reduced P loading due to implementation of the manure export BMP on a county scale. Under optimal conditions 198,000 kg manure P yr(-1) could be used and 114,840 kg manure P yr(-1) exported from the NBR watershed through implementation of a system using dairy manure to produce turfgrass sod. This is the equivalent of the manure P applied from 10,032 dairy cows yr(-1) and exported from 5808 dairy cows yr(-1). Application of GIS to large-scale planning and decision-making transcends traditional field-scale applications in precision agriculture.

Agriculture↗

Saltcedar control and water salvage on the Pecos river, Texas, 1999-2003.

A large scale ecosystem restoration program was initiated in 1997 on the Pecos River in Western Texas. Saltcedar (Tamarix spp.), a non-native invasive tree, had created a near monoculture along the banks of the river by replacing most native vegetation. Local irrigation districts, private landowners, federal and state agencies, and private industry worked together to formulate and implement a restoration plan, with a goal of reducing the effects of saltcedar and restoring the native ecosystem of the river. An initial management phase utilizing state-of-the-art aerial application of herbicide began in 1999 and continued through 2003. Initial mortality of saltcedar averaged about 85-90%. Monitoring efforts were initiated at the onset of the project to include evaluating the effects of saltcedar control on salinity of the river water, efficiency of water delivery down the river as an irrigation water source, and estimates of water salvage. To date, no effect on salinity can be measured and irrigation delivery was suspended in 2002-2003 due to drought conditions. Water salvage estimates show a significant reduction in system water loss after saltcedar treatment. However, a flow increase in the river is not yet evident. Monitoring efforts will continue in subsequent years.

Conservation of Natural Resources↗

Drug-resistant tuberculosis: a disease of target populations in Houston, Texas.

OBJECTIVES: To analyse the traditional and molecular epidemiology of drug-resistant tuberculosis (DRTB) in Houston and Harris County, Texas in the setting of decreasing disease incidence. METHODS: Case-control study of 193 patients with DRTB and 1977 patients with drug-susceptible TB (DSTB) identified from a population-based surveillance, 1995-2001. RESULTS: In a multivariate logistic regression, the following risk factors were found to be predictors of having DRTB (P< or =0.05): human immunodeficiency virus (HIV) seropositivity, Hispanic ethnicity, Asian ethnicity, history of past TB; whereas being foreign born, having a history of past TB, and younger age were predictors of MDRTB. There were 15 patients who acquired drug resistance while on therapy, and they were significantly more likely than controls to be HIV-seropositive, be of Asian ethnicity, have smear-positive pulmonary disease and present with pleural effusion on chest radiograph. No difference in 6-month mortality between DRTB and DSTB cases was found. During the study period, the incidence of DRTB remained constant. CONCLUSIONS: In Houston, there is a steady, low-level, incidence of DRTB which disproportionately affects specific subpopulations, with no evidence of increased mortality at 6 months.

Antitubercular Agents↗

Socioeconomic, cultural, and personal influences on health outcomes in low income Mexican-origin individuals in Texas.

Several studies have suggested that the health of Mexican-Americans is better than expected given their low socioeconomic status. The healthy migrant hypothesis and the acculturation hypothesis, stating that the foreign-born and the less acculturated enjoy better health, have been proposed as possible complementary explanations. However, it is not clear which are the socioeconomic, cultural, and personal characteristics that favor good health and that differentiate foreign-born from US-born and unacculturated from acculturated Mexicans. In this paper, we compare, by nativity and acculturation level, the socioeconomic, cultural, and personal characteristics in a sample of low income mostly female Mexican-origin individuals living in Texas and investigate their contribution to differences in self-reported physical health, mental health, and self-rated health (SRH) status. Using a multistage probability sample, we completed 1745 interviews with Mexican-origin individuals. The survey instrument included the SF-12, demographic and socioeconomic information, and questions on social support, religiosity, fear of victimization, trust, perceived racism, and perceived opportunity. Nativity and use of the Spanish language were combined into a nativity/acculturation variable. We estimated multivariate regressions and ordered logit regressions to investigate the association of health outcomes to nativity/acculturation and socioeconomic, cultural, and personal characteristics. Overall, the distribution of strengths (more social support, trust, perceived personal opportunities and less perceived victimization) reflected a nativity-based income gradient and an education gradient reflecting language use. Health outcomes varied by nativity/acculturation after controlling for socioeconomic, cultural, and personal characteristics. Physical health differed by nativity, supporting the healthy migrant hypothesis, while nativity-based differences in mental health were explained by socioeconomic and personal characteristics. SRH varied by language use, suggesting a culturally conditioned response. The socioeconomic, cultural, and personal factors affected health outcomes differently. These findings suggest a complicated interaction between nativity, acculturation, and economic factors in determining social and personal strengths and their influences on health.

Cultural Characteristics↗

Neighborhood economic conditions, social processes, and self-rated health in low-income neighborhoods in Texas: a multilevel latent variables model.

This paper develops and tests a comprehensive model to explain the relationships of neighborhood economic indicators to multiple dimensions of neighborhood social and physical organization as well as the pathways through which neighborhood social and physical characteristics influence individual health outcomes. We hypothesized that neighborhood poverty would be associated with lower collective efficacy, lower social capital, higher degrees of social and physical disorder, worse social processes pertaining to children such as trust, and higher degrees of fear of crime and racism. Neighborhood social and physical characteristics were hypothesized to mediate the effect of neighborhood poverty on self-rated health, both directly and indirectly through their influence on neighborhood differences in social support and health behaviors, which in turn affect individual health. The results, based on data from low-income neighborhoods in Texas, USA generally supported the model and indicated that the effect of neighborhood impoverishment on health is mediated by social and physical neighborhood characteristics.

Adolescent↗

The association of subjective social status and health in low-income Mexican-origin individuals in Texas.

There is only limited research on subjective social status (SSS) and its effect on health in general and in minority US populations in particular. This study first investigates the determinants of SSS and the relationship between SSS and objective social status. It then explores the relationships of SSS to self-reported physical health, self-reported mental health, and self-rated health (SRH). The study population consists of Mexican-origin individuals living in low-income neighborhoods in Texas and grouped into acculturation categories based on nativity and use of the Spanish language. We also investigate the role of reference groups. Results indicate that there is no disjuncture between subjective and objective status in this population but that the less acculturated groups rank their social status based on different criteria than the more acculturated. People compare themselves mainly with those similar to them and average subjective status in the different acculturation groups accurately reflects the objective status of the group. Sociocultural factors, in particular perceived differences in opportunities, explain differences between subjective and objective status. Subjective status was associated with all health outcomes. When controlling for objective status, subjective status was associated with mental health and SRH but not with physical health. Objective status indicators were consistently associated with all health outcomes, indicating the prominence of objective socioeconomic status in affecting health in this low-income minority population. Sociocultural characteristics appear to mediate the effect of SSS on health. In particular, perceived victimization might mediate the effect of SSS on mental health.

Acculturation↗

Pathogenesis of a Texas feline immunodeficiency virus isolate: an emerging subtype of clade B.

We have recently provided evidence that Texas feline immunodeficiency virus (FIV-TX) isolates are an emerging subtype sharing a common ancestry with clade B isolates. Specific, pathogen-free cats were infected, intravenously, with 500, 2000 or 8000TCID(50) of the FIV-TX53 virus to study the acute stage of infection. Infection of cats resulted in lymphadenopathy at 10 days post-infection (p.i.). By 7 weeks p.i., gag specific antibody could be detected from sera of all infected cats. Virus could be detected by culturing PBMC and by nested capsid PCR. A reduction in the absolute numbers of lymphocytes and neutrophils was observed in infected cats although there was no trend identified between this reduction and the viral dose administered. By 11 weeks p.i., the CD4(+)/CD8(+) T cell ratios from all infected cats had dropped from approximately 2 to below 1. While decrease in the ratio was dependent on the viral dose, the T cell ratios of cats receiving the highest dose had significantly dropped below 1 by 4-7 weeks p.i. This decrease in the ratio was accompanied by a sharp and temporal decline in the absolute CD4(+) T cells and a slight increase in the absolute CD8(+) T cell numbers with a dramatic expansion of cells with CD8beta(low) chain expression.

Acute Disease↗

Detection of Babesia bigemina infection in strains of Rhipicephalus (Boophilus) microplus collected from outbreaks in south Texas.

The sudden death of several cattle infested experimentally with Rhipicephalus (Boophilus) microplus led to a clinical investigation into the reasons for the unexpected mortality. Microscopic evidence for Babesia bigemina infection was found in blood smears from the affected animals and a PCR assay was designed to detect the presence of B. bigemina and Babesia bovis in all R. microplus strains received and propagated at the laboratory. The assay utilizes a nested PCR approach with the first PCR amplifying a well-conserved segment from the Babesia 18S ribosomal RNA gene followed by a nested PCR with Babesia species-specific primers and annealing temperatures enabling amplification of the 18S ribosomal RNA gene fragment specific to either B. bigemina or B. bovis. DNA from groups of 50 larvae was extracted using a rapid DNA preparation protocol, which consisted of grinding the frozen tick larvae in PCR buffer and boiling the mixture for 5min. The assay sensitivity allowed for the detection of the equivalent of a single infected tick larva. R. microplus eggs were also analyzed, but yolk protein viscosity created inconsistent results with the crush and boil DNA isolation protocol, necessitating the use of a more extensive proteinase K digestion-based DNA purification method. We detected the presence of B. bigemina in all strains of R. microplus currently reared at the laboratory and 4 of 26 strains collected from infestation outbreaks in Texas by the U.S. Cattle Fever Tick Eradication Program.

Animals↗

Emergence of attenuated West Nile virus variants in Texas, 2003.

In order to understand how West Nile virus (WNV) has evolved since its introduction into North America, we have studied the genetic and phenotypic variation among WNV isolates collected in various areas during consecutive transmission seasons. The present report describes for the first time phenotypic changes occurring in the North American WNV population. Several isolates collected in Texas during 2003 display a small plaque (sp) and temperature sensitive (ts) phenotype, as well as reduced replication in cell culture, in comparison to isolates collected in 2002 and New York in 1999. Studies of mouse neuroinvasiveness/neurovirulence also indicate that several of these isolates were attenuated in neuroinvasiveness, but not for neurovirulence. The complete genome and deduced amino acid sequences of several of these isolates have been determined in order to map the mutations responsible for this phenotypic variation. These data indicate microevolution of WNV and the emergence of isolates exhibiting phenotypic variation.

Animals↗

Economics of dairy waste use as fertilizer in central Texas.

Dairy manure is an unavoidable natural, but negative, byproduct of milk production. Its nitrogen, phosphate, and potash contents represent a potential substitute for commercial fertilizers on field crops. In the absence of subsidies, manure transportation and land application costs limit its utilization as a substitute for chemical fertilizer. The results from a study of the economics of manure use in Central Texas suggest that, at the current costs for loading, hauling, and spreading, dairy manure cannot be economically transported from surplus to deficit areas within the study area. The estimated breakeven transport distance for manure application to four crops varied from 28 to 41 km; however, the distances between manure-surplus and manure-deficit counties in the study region varied from 40 to 90 km. An analysis of potential subsidies paid by the government or dairy farmers showed that the breakeven distance could increase by up to 30 km. A decrease in the assumed moisture content of the manure from 50% to 40% is shown to increase the breakeven distance by 10 km. The study suggests that dairy manure loading, transportation, and land application, with appropriate subsidies or reductions in moisture content, has the potential to be profitably substituted for chemical fertilizers.

Agriculture↗