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At least 685 records · Page 38Linked to original sources
PubMed1984

Expression of rabbit ventricular alpha-myosin heavy chain messenger RNA sequences in atrial muscle.

We have constructed and isolated a cardiac myosin heavy chain (HC) cDNA clone, pMHC alpha 81, with mRNA from ventricular heart muscle of hyperthyroid rabbits. The clone encodes approximately 480 amino acids of the COOH terminus of light meromyosin and all of the 3' nontranslated region of the corresponding mRNA. Nuclease S1 analyses indicated that the clone is transcribed in hyperthyroid, but not in hypothyroid ventricles and, therefore, corresponds to ventricular alpha-HC mRNA. With probes from the more divergent 3' non-translated region of pMHC alpha 81 and also from selected portions of two previously characterized rabbit cDNA clones ( pMHC alpha 252 and pMHC beta 174), we analyzed the myosin HC mRNAs of atrial, fast skeletal, and slow skeletal muscles by nuclease S1 mapping. In atrial muscle, only one major transcript was detected. The sequence of this transcript was indistinguishable from ventricular alpha-HC mRNA in the 3' nontranslated region and in two coding segments. In contrast, the sequence divergence between the ventricular alpha-HC mRNA and the mRNAs of ventricular beta, fast skeletal, and slow skeletal myosin HCs was clearly detected. There appeared to be, however, considerable homology between coding sequences of ventricular beta and slow skeletal myosin HC mRNAs. The results strongly suggest that rabbit atrial and ventricular alpha-HCs are encoded by the same gene.

Amino Acid Sequence↗
PubMed1981

Cotton messenger RNA sequences exist in both polyadenylated and nonpolyadenylated forms.

The physical characteristics and sequence content of polyadenylated and nonpolyadenylated messenger RNAs were determined in total and polysomal RNAs isolated from cotton cotyledons after 24 h of germination. RNAs were fractionated on the basis of their poly(A) content by passage through poly(U)-agarose. The mass average size of poly(A) is 112 +/- 4 nucleotides in total RNA and 81 +/- 3 nucleotides in polysomal RNA. Less than 2% of the poly(A) tracts are between 5-15 nucleotides in length. The mass average length of recovered polyadenylated mRNAs from either RNA is 1600 nucleotides. In vitro translation of the RNAs in the wheat germ system and hybridization of the RNAs with complementary DNAs confirmed the physical estimates of their absolute amounts. About 3.3% of the high molecular weight RNA in the tissue is mRNA, of which 40% is on polysomes. Abut 45% of the polysomal mRNA and 75% of the nonpolysomal mRNA is polyadenylated. These mRNAs are transcribed from nonrepetitive DNA. Both techniques unambiguously demonstrate that all mRNA populations contain the same sequences in about the same relative concentration. At this point of development in this tissue, differential polyadenylation or entry into polysomes cannot be specific for particular mRNA sequences.

Gossypium↗
PubMed2006

Analysis of environmental 18S ribosomal RNA sequences reveals unknown diversity of the cosmopolitan phylum Telonemia.

Telonemia has recently been described as a new eukaryotic phylum with uncertain evolutionary origin. So far, only two Telonemia species, Telonema subtilis and Telonema antarcticum, have been described, but there are substantial variations in size and morphology among Telonema isolates and field observations, indicating a hidden diversity of Telonemia-like species and populations. In this study, we investigated the diversity and the global distribution of this group by analyzing 18S rDNA sequences from marine environmental clone libraries published in GenBank as well as several unpublished sequences from the Indian Ocean. Phylogenetic analyses of the identified sequences suggest that the Telonemia phylum includes several undescribed 18S rDNA phylotypes, probably corresponding to a number of different species and/or populations. The Telonemia phylotypes form two main groups, here referred to as Telonemia Groups 1 and 2. Some of the closely related sequences originate from separate oceans, indicating worldwide distributions of various Telonemia phylotypes, while other phylotypes seem to have limited geographical distribution. Further investigations of the evolutionary relationships within Telonemia should be conducted on isolated cultures of Telonema-like strains using multi-locus sequencing and morphological data.

Animals↗