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At least 685 records · Page 38Linked to original sources
PubMed2004

Comparative effectiveness of cattle manure, poultry manure, phosphocompost and fertilizer-NPK on three cropping systems in vertisols of semi-arid tropics. II. Dry matter yield, nodulation, chlorophyll content and enzyme activity.

A field experiment was conducted on a deep Vertisol of Bhopal, India to compare root and shoot biomass, chlorophyll content, enzyme activity and nodulation in three cropping systems at three combinations of organic manure and inorganic-fertilizer: 75%NPK + 5 t farmyard manure (FYM), 75%NPK + 1.5 t poultry manure (PM), and 75%NPK + 5 t phosphocompost (PC) vis-a-vis 0%, 75% and 100% of fertilizer-NPK. In general, nodule number and its mass were lower in intercrop soybean than sole soybean. Also there was decrease in the nodule number with higher NPK dose. The FYM treated plots recorded 22.0% and 7.6% higher nodule mass than poultry manure and phosphocompost plots, respectively. Also, the total chlorophyll content was higher in organically treated plots than that in 100% NPK particularly at 30 days after sowing (DAS, pre-flowering). In sorghum the peak nitrate reductase (NR) activity was recorded at 60 DAS while in soybean it was at 30 DAS. The NR activity was higher in intercrop sorghum than that in sole sorghum. Maximum NR activity was observed in 100% NPK. Soybean/sorghum intercropping system recorded significantly higher root and shoot biomass than sole soybean and sorghum. The crop growth rates were relatively rapid during 30-60 DAS and followed the order; intercropping > sole sorghum > sole soybean. With the increase in NPK dose from 0% to 100% there was significant improvement in the dry matter (DM) production in sole sorghum and soybean/sorghum intercropping system. Soybean as preceding crop recorded the highest DM, chlorophyll content, NR activity in wheat while these values were the lowest in sorghum-wheat system.

Agriculture↗
PubMed2005

Effect of processing time on enteric bacteria survival and on temperature and chemical composition of broiler poultry litter processed by two methods.

This study was conducted to evaluate the effects of deepstack processing broiler poultry litter with or without aeration on enteric bacteria survival (Trial 1), and to determine the optimum aeration period (Trial 2) and aeration frequency (Trial 3) to control excessive heating of litter during the deepstacking process. The effects of deepstacked litter temperature on bacteria survival and on chemical compositions of the litter were monitored. In Trial 1, E. coli, Salmonella enteriticis and Shigella sonnei were intentionally inoculated into the litter and their survival was monitored with time of litter processing. In Trial 2, litter was deepstacked and aerated once a day from the initial day or from the day when maximum stack temperature was reached and started to decrease to the end of processing period. In Trial 3, litter was deepstacked and aerated once or twice a day from the day after maximum stack temperature was attained until constant temperature was attained. During the deepstacking process of litter pathogenic bacteria were eliminated between the 2nd and 4th day of deepstacking. This phenomenon occurred apparently not only by high heat generated, but also by other potential factors, such as ammonia and microbial competition. Aeration resulted in the heat dissipation and early temperature reduction of deepstacked litter. The optimum aeration procedure to avoid excessive heating of deepstacked litter was to aerate once a day after the stack temperature reached its maximum at the early stage of deepstacking and for 3 days or until the stack temperature dropped off and reached constancy. This aerating frequency and duration did not negatively affect the chemical composition of deepstacked litter. These results suggest that poultry litter properly deepstacked, with or without aeration, to ensure the elimination of enteric bacterial pathogens may be fed at least 10 days earlier than the 21-day deepstacking period previously recommended.

Air↗
PubMed2005

Effect of formic acid exposure on keratin fiber derived from poultry feather biomass.

Converting poultry feather biomass into useful products presents a new avenue of utilization of agricultural waste material. However, not much is understood about the poultry feather structure or methods to process it. In this study, formic acid vapor is systematically allowed to penetrate the feather fiber structure, which is composed of keratin. The diffusion kinetics show Fickian behavior during absorption. After very long times, i.e., greater than 10(3)h, the absorption experiments are stopped and the formic acid is allowed to desorb from the keratin material. The desorption kinetics of formic acid out of the keratin fiber do not mirror the absorption kinetics, indicating a change in the keratin microstructure. DSC and NMR spectroscopy analyses on the keratin fiber show a reduction in the area of the crystalline melting peak and solubilization of amino acids upon formic acid exposure. This indicates that the crystallinity is disrupted resulting in more amorphous fraction in the keratin polymer.

Animals↗
PubMed2006

Evaluation of isopathic treatment of Salmonella enteritidis in poultry.

BACKGROUND: Salmonellosis is a common problem worldwide in commercially reared poultry. It is associated with human Salmonellosis. No fully satisfactory method of control is available. METHOD: Nosodes to an antibiotic-resistant strain of Salmonella enterica serovar Enteritidis in D30 (30X) potency were prepared. One day old chicks (N = 180) were divided into four groups: two control and two different preparations of the nosode. Treatments were administered in drinking water for 10 days. The birds were challenged by a broth culture of the same Salmonella, by mouth, on day 17. Cloacal swabs were taken twice weekly for Salmonella enterica serovar Enteritidis. RESULTS: Birds receiving active treatment were less likely to grow the strain of Salmonella from cloacal swabs compared to control. CONCLUSION: Isopathy is low cost and non-toxic. It may have a role to play in the widespread problem of Salmonella in poultry. Further research should be conducted.

Administration, Oral↗
PubMed2005

Antimicrobial resistance in Salmonella enteritidis strains isolated from broiler carcasses, food, human and poultry-related samples.

Antimicrobial resistance was investigated in 91 Salmonella enteritidis isolates from broiler carcasses, food, human and poultry-related samples originated from South of Brazil. A great proportion of resistant strains was found, 90.1% showing resistance to at least one antimicrobial drug. There was a high resistance to sulfonamides (75.8%) and nitrofurantoin (52.8%). Lower levels of resistance were found for tetracycline (15.4%), streptomycin (7.7%), nalidixic acid (7.7%), gentamicin (5.5%), norfloxacin (3.3%), trimethoprim (3.3%), cefalotin (2.2%), ampicillin (1.1%), and chloramphenicol (1.1%). Resistance to ciprofloxacin was not detected. A total of 51.6% of S. enteritidis strains were multiresistant (resistance to two or more antimicrobial agents) and 18 resistance patterns were found. The highest resistance was found in strains isolated from poultry-related samples, where all strains were resistant to at least one antimicrobial agent. No predominant resistance pattern was related to phage type in our isolates. The high number of antimicrobial resistant S. enteritidis found in Southern Brazil indicates the need for the prudent drugs uses to diminish the development and spread of antimicrobial resistance.

Animals↗
PubMed2005

Antagonistic activity against Salmonella infection in vitro and in vivo for two Lactobacillus strains from swine and poultry.

In this study, we isolated two lactobacillus strains, i.e., strain LAP5 and LF33, from swine and poultry, respectively, and showed that both strains were acid as well as bile tolerant and were able to adhere to the cultured human intestinal cell lines, such as Int-407 and Caco-2 cells, and to the intestinal epithelium cells isolated from swine, poultry and mouse (BALB/c). Both of these LAB strains were shown to inhibit the growth of Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Bacillus cereus. When these two LAB strains were evaluated for their antagonistic activity against Salmonella (S. typhimurium) invasion to cultured human intestinal cell line Int-407 and to mouse (BALB/c) liver and spleen, these two LAB strains were found to have significant antagonistic effect.

Animals↗
PubMed2006

Detection and diversity of various Arcobacter species in Danish poultry.

The prevalence and diversity of different Arcobacter spp. in various poultry species in Denmark were investigated using cultural and multiplex PCR methods. A pool of three fresh droppings obtained at the production site from 70 broiler chicken flocks aged 4-5 weeks was examined. In addition, pools of 10 cloacal swabs taken at the abattoir prior to stunning from each of 15, and 37 duck and turkey flocks, respectively, were analyzed. Thirty fresh broiler chicken carcasses and 29 cloacal swabs from the respective viscera were also examined at the abattoir. Finally, 10 caecal and 10 cloacal swabs from ducks at the abattoir were analyzed individually. In total, 85 Arcobacter isolates were obtained. Of these 45, 20 and 7 were identified as Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii, respectively, using a multiplex PCR. Interestingly, some chicken isolates of A. butzleri showed urease activity, and 6 out of seven A. skirrowi isolates were unable to hydrolyse indoxyl acetate. All chicken carcasses examined were found positive for A. butzleri and/or A. cryaerophilus, whereas 21 (72%) of the 29 chicken cloacal swabs were positive for either A. butzleri (13) or A. cryaerophilus (9). Three (4.3%) out of 70 chicken flocks analyzed were positive only for A. cryaerophilus. Of the ten ducks examined individually, 7 carried A. skirrowii and/or A. cryaerophilus in their cloacae. None of the respective caecal samples were positive. Of the remaining 15 duck flocks, 11 (73%) were positive for A. cryaerophilus (7), A. butzleri (2) or A. skirrowii (2). Four (11%) of the 37 turkey flocks analyzed harboured either A. butzleri or A. cryaerophilus. The carriage rate of Arcobacter was higher in live ducks than those of live broiler chickens and turkeys in the present study. In addition, chicken carcasses slaughtered in Denmark were found to be contaminated with Arcobacter. The presence of Arcobacter spp. both on chicken carcasses and in poultry intestine may be of significance to human health.

Abattoirs↗
PubMed2026

Whole-genome sequencing links a Salmonella Newport ST164 outbreak on Fernando de Noronha to prior circulation in the Brazilian poultry supply chain.

Foodborne outbreaks at geographically isolated tourist destinations pose distinctive One Health challenges, combining limited local surveillance capacity, complex intercontinental supply chains, and high visitor turnover. In May 2021, a diarrheal outbreak linked to a gastronomic festival in Fernando de Noronha, that is a remote UNESCO World Heritage island off northeastern Brazil, was attributed to Salmonella enterica serovar Newport ST164. We applied an integrated genomic approach and epidemiological investigation to propose a transmission chain contextualizing and refining case definition of the S. Newport epidemic clone within national and international diversity. Whole-genome sequencing (WGS), SNP-based phylogenomic, pangenome analysis, Salmonella pathogenicity island (SPI) profiling, and resistome characterization was performed on 17 epidemiologically attributed outbreak isolates and 68 contextual genomes from Brazil, France, the United Kingdom, and the United States. The SNP analysis identified a 13 genome clonal core with less than 20 different SNPs demonstrating the possible connection between 9 patient isolates, 2 food isolates, and 2 food handler isolates, consistent with the involvement of colonised kitchen staff in cross-contamination of the ready-to-eat mussel dish. Three poultry isolates in 2020 from a mainland producer, ∼2180 km from Fernando de Noronha, differed only 13 to 17 Core-SNPs from the outbreak core, suggesting prior lineage circulation in the supply chain. Pangenome analysis also supports this evidence revealing near-complete genomic overlap of 4544 shared genes within the 5745 gene clusters (99.9%) between outbreak and non-outbreak backgrounds that mostly differentiate by a defense/prophage-associated accessory module. The resistome comprised intrinsic efflux determinants without acquired resistance and showed 35.3% of intermediate ciprofloxacin susceptibility. This One Health based study provides a WGS genomic reconstruction of a S. Newport ST164 outbreak at a remote tourist island, supporting the possibility of circulation from poultry-associated mainland reservoirs and findings consistent with cross-contamination at a gastronomic seafood festival.

Brazil↗
PubMed2005

Analysis of the 1999-2000 highly pathogenic avian influenza (H7N1) epidemic in the main poultry-production area in northern Italy.

We evaluated the effects of risk factors and control policies following the highly pathogenic avian influenza (HPAI) epidemic that struck northern Italy's poultry industry in the winter of 1999-2000. The epidemic was caused by a type-A influenza virus of the H7N1 subtype, that originated from a low-pathogenic AI virus which spread among poultry farms in northeastern Italy in 1999 and eventually became virulent by mutation. Most infected premises (IP) were located in the regions of Lombardy and Veneto (382 out of 413, 92.5%), and the eradication measures provided for in the European legislation were enforced. In Veneto, where flock density was highest, infection-control was also accomplished by means of depopulation of susceptible flocks through a ban on restocking and pre-emptive slaughter of flocks that were in the vicinities of or that had dangerous contacts with IPs. In Lombardy, such control measures were applied to a lesser extent. Infection incidence rate (IR) was 2.6 cases per 1000 flocks per day in Lombardy and 1.1 in Veneto. After the implementation of infection-control measures, the at-risk population, the percentage of flocks < or =1.5 km from IPs, and the HPAI-IR underwent a greater reduction in Veneto than in Lombardy. Although the proximity (< or =1.5 km) to IPs in the temporal risk window (TRW) was a major risk factor for HPAI at the individual flock level, its effect at the population level (population-attributable fraction) did not exceed 31.3%. Viral transmission therefore also occurred among relatively distant flocks. Turkey flocks were characterised by greater IR of HPAI compared with other bird species such as layer hens, broilers, gamebirds, and waterfowl, even when located at distances >1.5 km from IPs. In Lombardy, IR for species other than turkeys was also relatively high.

Animals↗
PubMed2005

Stakeholder position paper: poultry.

There has been renewed concern in recent years about the use of antibiotics in food animal production and the potential risk it may pose to public health due to transfer of antibiotic resistance factors via the food supply. Although a legitimate concern, it bears reminding that this debate is not new. It has been ongoing for decades, yet there is still no documented case of human treatment failure due to antibiotic resistant bacteria acquired from USDA-inspected meat and poultry. This fact strongly suggests that the issue is not the imminent threat as has been portrayed by certain individuals or advocacy groups. The poultry industry as a whole has been using antibiotics responsibly for several decades, and there are strong beneficial arguments for their continued use. Responsible public policy demands a science-based approach be utilized in the decision making process before attempting to restrict or remove certain products due to overestimated risks. Part of this scientific review should include antibiotic use data, however this information has definite limitations and shortcomings which need to be understood before attempting to make any valid antibiotic resistance associations.

Animals↗
PubMed2005

Antimicrobial susceptibility of Enterococcus strains isolated from poultry faeces.

We have investigated the resistance of Enterococcus isolated from poultry faeces to antibiotics commonly used as therapy of enterococcal infections. Identification was made by the method of Facklam and Collins. Minimal inhibitory concentrations of penicillin, ampicillin, vancomycin and teicoplanin were determined and high level aminoglycoside resistance was investigated. Genes codifying high level aminoglycoside resistance (HLAR) were determined by PCR. Fifty five Enterococcus strains were isolated (63.6% E. faecalis, 12.7% E. mundtii, 9.1% E. faecium, 7.3% E. casseliflavus, 3.7% E. durans and 3.6% E. hirae). None of the strains were resistant to VAN, TEC, P or AM. HLAR was found in 34.5% of strains for SM, 27.3% for KM and 7.3% for GM. The gene for the bifunctional enzyme was found only in one strain, that showed HLAR to GM and KM. Fourteen strains harboured the gene aph(3')-III, being 11 resistant to KM and STR, and three resistant to GM, KM and STR. The remaining six strains showed HLAR to STR, but were negative for the three genes tested by PCR. The gene ant(4'4") was not detected in any of the strains. No unexpected vancomycin resistance was detected. The resistance rates among poultry strains were lower than those found among human strains isolated from hospital patients in recent Canary studies.

Animals↗
PubMed2005

Comparison of vancomycin-resistant enterococci isolates from human, poultry and pigs in Korea.

Vancomycin-resistant enterococci (VRE) have emerged as an important nosocomial pathogen. Since 1989, a rapid increase in the incidence of enterococcal bacteremia and endocarditis by VRE has been reported. The use of avoparcin in animal husbandry is reportedly associated with the appearance of VRE. In this study, a multiplex polymerase chain reaction (PCR) method was established to detect and differentiate resistant types of enterococci, which specifically amplify the four van genes that encode vancomycin resistance elements. Using this method, we investigated the incidence rates and types of VRE from two types of farms: those that had used avoparcin and those that had not used avoparcin. A total of 1091 animal fecal samples were collected from 70 pig farms and 32 poultry farms. A total of 425 enterococci were isolated from the fecal samples. Among the 425 isolates, six showed a pattern of high-level vancomycin resistance (Minimal Inhibitory Concentration, MIC: 64-256 microg/ml). Out of six high-level VRE, three were isolated from poultry farms that had used avoparcin and three were not. The six high-level VRE harbored the vanA gene. Sixty-seven of 425 isolates that showed a pattern of low-level vancomycin resistance (MIC: 4-8 microg/ml) were associated with the presence of vanC-1 or vanC-2/3 gene. We also performed a repetitive extragenic palindromic PCR (rep-PCR) method to compare the genetic relatedness between the high-level VRE of six animal isolates and 31 human isolates. None of the animal isolates had a similar rep-PCR pattern as the human isolates but similarities between human VRE isolates were observed.

Animals↗
PubMed2006

ESBL- and plasmidic class C beta-lactamase-producing E. coli strains isolated from poultry, pig and rabbit farms.

This study aims to determine the presence of extended-spectrum (ESBL) and plasmidic class C beta-lactamase-producing Enterobacteriaceae in poultry, pig and rabbit farms of Catalonia (Spain). PFGE typing showed a low clonal relationship among strains carrying these mechanisms of resistance. Ninety-three percent of them were resistant to two or more of the non-beta-lactam antimicrobials tested and harboured ESBL and plasmidic class C beta-lactamases. Greater diversity of these enzymes was found in strains from poultry farms, the CTX-M-9 family, especially CTX-M-14, with CMY-2 being the most frequent. The isolation of TEM-52 and SHV-2-producing Escherichia coli strains from these animal farms is noteworthy. In contrast, 73% of the strains from pig farms had CTX-M-1, and neither the CMY-type nor CTX-M-9 family enzyme was found. Likewise, it is the first time that CTX-M-1 and SHV-5 encoding strains have been isolated in pigs. On the other hand, in rabbit farms CTX-M-9 family was also the most frequent, being detected in three of a total of four strains. The last one showed a CMY-2, for the first time detected in these animals, too. In conclusion, commensal E. coli strains of food-producing animal farms are a reservoir of ESBL and plasmidic class C beta-lactamases.

Animals↗
PubMed2000

Residues of tetracycline compounds in poultry products in the eastern province of Saudi Arabia.

Chicken muscle, liver and egg samples were collected from 33 broiler and 5 layer farms in the eastern province of Saudi Arabia over a period of two years starting from January 1996. Antibiotic-residue positive samples were identified in the products of 23 (69.7%) broiler and 3 (60%) layer poultry farms. 87% and 100.0% of the antibiotic-residue positive broiler farms were positive for at least one tetracycline compound in raw muscle and liver respectively, while 73.9% and 95.5% were positive for 2 or more tetracyclines in these two tissues, respectively. Furthermore, 82.6% and 95.5% of the antibiotic-residue-positive farms had mean concentrations of at least one tetracycline compound in excess of the permissible maximum residue limit (MRL) in raw muscle and liver, respectively. These compounds also remained chemically detectable after cooking. Tetracycline levels exceeded MRL in 14.4% of antibiotic-positive raw eggs but the overall mean tetracycline concentration in each farm was below MRL. This study confirmed widespread misuse of tetracycline agents including multiple use of drugs belonging to the same pharmacological group and lack of implementation of recommended withdrawal times. This may be contributing to the high resistance rates to tetracyclines in both chicken and human microbial isolates observed in the region. This study, therefore, stresses the need for stricter regulations for the use of antimicrobial drugs in the poultry industry as well as the inspection of chicken for drug residues prior to marketing.

Agriculture↗
PubMedDate not supplied

Gait analysis of poultry.

Lameness is a major problem in the UK poultry industry, however, relatively few objective studies have been undertaken into the biomechanics of normal walking in these birds. In this study, the use of a pedobarograph as a novel method of gait analysis in poultry was investigated. Unlike most systems, the pedobarograph has a recording surface with a high degree of spatial resolution, allowing pressure patterns to be established for various regions of the foot. The highest pressures were found to act on the medial toe (149.4-218 kN m(-2)) and back toe (146.1-195.5 kN m(-2)). The metatarsal pad, a region often associated with lesions, was subject to lower pressures (16.3-131.2 kN m(-2)). Maximum net forces of 116-145 per cent of bodyweight were found during normal walking, an order of the same magnitude as human bipeds. Routine spatial parameters were also measured, allowing further characterisation of the gait patterns.

Animals↗
PubMed2002

Antimicrobial residues in animal waste and water resources proximal to large-scale swine and poultry feeding operations.

Expansion and intensification of large-scale animal feeding operations (AFOs) in the United States has resulted in concern about environmental contamination and its potential public health impacts. The objective of this investigation was to obtain background data on a broad profile of antimicrobial residues in animal wastes and surface water and groundwater proximal to large-scale swine and poultry operations. The samples were measured for antimicrobial compounds using both radioimmunoassay and liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS) techniques. Multiple classes of antimicrobial compounds (commonly at concentrations of > 100 microg/l) were detected in swine waste storage lagoons. In addition, multiple classes of antimicrobial compounds were detected in surface and groundwater samples collected proximal to the swine and poultry farms. This information indicates that animal waste used as fertilizer for crops may serve as a source of antimicrobial residues for the environment. Further research is required to determine if the levels of antimicrobials detected in this study are of consequence to human and/or environmental ecosystems. A comparison of the radioimmunoassay and LC/ESI-MS analytical methods documented that radioimmunoassay techniques were only appropriate for measuring residues in animal waste samples likely to contain high levels of antimicrobials. More sensitive LC/ESI-MS techniques are required in environmental samples, where low levels of antimicrobial residues are more likely.

Animal Feed↗
PubMed2001

Bacterial inoculum enhances keratin degradation and biofilm formation in poultry compost.

Native microbial populations can degrade poultry waste, but the process can be hastened by using feather-degrading bacteria. Strains of Bacillus licheniformis and a Streptomyces sp. isolated from the plumage of wild birds were grown in a liquid basal medium and used to inoculate feathers in compost bioreaction vessels. Control vessels had only basal medium added to the feathers, litter and straw. Temperature, ammonia, carbon and nitrogen were monitored for 4 weeks. Scanning electron microscopy of the feather samples showed more complete keratin-degradation, more structural damage, and earlier microbial biofilm formation on inoculated feathers than on uninoculated feathers. A diverse community of aerobic bacteria and fungi were cultured early, but declined rapidly. Thermophilic B. licheniformis and Streptomyces spp. were abundant throughout. Enteric gram-negative bacteria, (e.g., Salmonella, E. coli) originally found on waste feathers were not recovered after day 4. Vessel temperatures reached 64-71 degrees C within 36 h and stabilized at 50 degrees C. When tumble-mixed at day 14, renewed activity peaked at 59 degrees C and quickly dropped as available carbon was used. Feathers soaked in an inoculum of B. licheniformis and Streptomyces degraded more quickly and more completely than feathers that were not presoaked. Inoculation of feather waste could improve composting of the large volume of feather waste generated every year by poultry farms and processing plants.

Animals↗
PubMed1997

Evaluation of a new enrichment broth for the isolation of Salmonella spp. from poultry products.

Four selective enrichment broths were compared for the detection of Salmonella spp. in naturally contaminated poultry products and the recovery of atypical Salmonella strains in suspensions of pure cultures. In analysis of 100 poultry samples, the sensitivities observed were 94.0% for Müller-Kauffmann Tetrathionate-Brilliant Green (MKTBG), 97.6% for Rappaport Vassiliadis (RV), 42.2% for Selenite Cystine (SC) and 97.6% for the new broth KIMAN (Whitley Impedance Broth supplemented with 20 mg/l of novobiocin sodium salt, 10 mg/l of malachite green oxalate and 40 g/l of potassium iodide). The two most efficient broths--RV and KIMAN for recovery of atypical Salmonella strains (gallinarum biotypes gallinarum and pullorum, typhi, paratyphi A) were less toxic than MKTBG but more toxic than s.c. broth. According to these results, the use of RV and KIMAN could be a good combination to assure maximal recovery of Salmonella strains.

Animals↗