Population data in social science data archives: the survey holdings of the Roper Public Opinion Research Center.
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Archives↗
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Promotion and evaluation of a virtual live reference service.
The University of North Carolina at Chapel Hill Health Sciences Library began providing virtual live reference service in the fall of 2001. The library was interested in exploring new ways to interact with users accessing electronic resources from outside the library. This article describes the background for starting the service, the methods used to promote the service, and the results of an evaluation conducted after the first seven months of operation. The service was lightly used during these months with only 82 interactions. An analysis of the transcripts examined referring Web pages, types of questions asked, software features used, and user login data. Text of two user surveys is included, with the data from the in-person survey analyzed.
Computer Communication Networks↗
GRATEFUL MED searching: a hospital library perspective.
The Health Sciences Information Center of Cedars-Sinai Medical Center implemented an end user search service utilizing NLM's GRATEFUL MED software. A librarian-taught seminar is required of all end users. The course's teaching strategies are described. A survey of users was conducted and results are shared.
Computer User Training↗
[A survey of the best bibliographic searching system in occupational medicine and discussion of its implementation].
When occupational health personnel, especially occupational physicians search bibliographies, they usually have to search bibliographies by themselves. Also, if a library is not available because of the location of their work place, they might have to rely on online databases. Although there are many commercial databases in the world, people who seldom use them, will have problems with on-line searching, such as user-computer interface, keywords, and so on. The present study surveyed the best bibliographic searching system in the field of occupational medicine by questionnaire through the use of DIALOG OnDisc MEDLINE as a commercial database. In order to ascertain the problems involved in determining the best bibliographic searching system, a prototype bibliographic searching system was constructed and then evaluated. Finally, solutions for the problems were discussed. These led to the following conclusions: to construct the best bibliographic searching system at the present time, 1) a concept of micro-to-mainframe links (MML) is needed for the computer hardware network; 2) multi-lingual font standards and an excellent common user-computer interface are needed for the computer software; 3) a short course and education of database management systems, and support of personal information processing for retrieved data are necessary for the practical use of the system.
Computers↗
Prevalence of honorary and ghost authorship in Cochrane reviews.
CONTEXT: To determine the prevalence of honorary and ghost authorship in Cochrane reviews, how authorship is assigned, and the ways in which authors and Cochrane editorial teams contribute. METHODS: Using a Web-based, self-administered survey, corresponding authors for 577 reviews published in issues 1 and 2 from 1999 of The Cochrane Library were invited to report on the prevalence of honorary and ghost authors, contributions by authors listed in the byline and members of Cochrane editorial teams, and identification of methods of assigning authorship. Responses were received for 362 reviews (63% response rate), which contained 913 authors. RESULTS: One hundred forty-one reviews (39%) had evidence of honorary authors, 32 (9%) had evidence of ghost authors (most commonly a member of the Cochrane editorial team), and 9 (2%) had evidence of both honorary and ghost authors. The editorial teams contributed in a wide variety of ways to 301 reviews (83%). Authorship was decided by the group of authors (31%) or lead author (25%) in most reviews. Authorship order was assigned according to contribution in most reviews (76%). The 3 functions contributed to most by those listed in the byline were assessing the quality of included studies (83%), interpreting data (82%), and abstracting data from included studies (77%). CONCLUSIONS: A substantial proportion of reviews had evidence of honorary and ghost authorship. The Cochrane editorial teams contributed to most Cochrane reviews.
Authorship↗
Electronic submission of academic works: a survey of current editorial practices of radiologic journals.
Computers are nearly ubiquitous in academic medicine, and authors create and compile much of their work in the electronic environment, yet the process of manuscript submission often fails to utilize the advantages of electronic communication. The purpose of this report is to review the submission policies of major academic journals in the field of radiology and assess current editorial practices relating to electronic submission of academic works. The authors surveyed 16 radiologic journals that are indexed in the Index Medicus and available in our medical center library. They compared the manuscript submission policies of these journals as outlined in recent issues of the journals and the corresponding worldwide web sites. The authors compared the journals on the following criteria: web site access to instructions; electronic submission of text, both with regard to initial submission and final submission of the approved document; text hardcopy requirements; word processing software restrictions; electronic submission of figures, figure hardcopy requirements; figure file format restrictions; and electronic submission media. Although the trend seems to be toward electronic submission, there currently is no clear-cut standard of practice. Because all of the journals that accept electronic documents also require a hardcopy, many of the advantages gained through electronic submission are nullified. In addition, many publishers only utilize electronic documents after a manuscript has been accepted, thus utilizing the benefits of digital information in the printing process but not in the actual submission and peer-review process.
Humans↗
Timely translation during the mouse oocyte-to-embryo transition.
In the mouse, completion of oocyte maturation and the initiation of preimplantation development occur during transcriptional silence and depend on the presence and translation of stored mRNAs transcribed in the growing oocyte. The Spin gene has three transcripts, each with an identical open reading frame and a different 3' untranslated region (UTR). (Beta)-galactosidase-tagged reporter transcripts containing each of the different Spin 3'UTRs were injected into oocytes and zygotes and (beta)-galactosidase activity was monitored. Results from these experiments suggest that differential polyadenylation and translation occurs at two critical points in the oocyte-to-embryo transition - upon oocyte maturation and fertilization - and is dependent on sequences in the 3'UTR. The stability and mobility shifts of ten other maternal transcripts were monitored by reprobing a northern blot of oocytes and embryos collected at 12 hour intervals after fertilization. Some are more stable than others and the upward mobility shift associated with polyadenylation correlates with the presence of cytoplasmic polyadenylation elements (CPEs) within about 120 nucleotides of the nuclear polyadenylation signal. A survey of the 3' UTRs of expressed sequence tag clusters from a mouse 2-cell stage cDNA library indicates that about one third contain CPEs. We suggest that differential transcript stability and a translational control program can supply the diversity of protein products necessary for oocyte maturation and the initiation of development.
3' Untranslated Regions↗
Molecular identification of potential pathogens in water and air of a hospital therapy pool.
Indoor warm-water therapy pool workers in a Midwestern regional hospital were diagnosed with non-tuberculosis pulmonary hypersensitive pneumonitis and Mycobacterium avium infections. In response, we conducted a multiseason survey of microorganisms present in this therapy pool water, in biofilms associated with the pool containment walls, and in air immediately above the pool. The survey used culture, microscopy, and culture-independent molecular phylogenetic analyses. Although outfitted with a state-of-the-art UV-peroxide disinfection system, the numbers of bacteria in the therapy pool water were relatively high compared with the potable water used to fill the pool. Regardless of the source, direct microscopic counts of microbes were routinely approximately 1,000 times greater than conventional plate counts. Analysis of clone libraries of small subunit rRNA genes from environmental DNA provided phylogenetic diversity estimates of the microorganisms collected in and above the pool. A survey of >1,300 rRNA genes yielded a total of 628 unique sequences, the most common of which was nearly identical to that of M. avium strains. The high proportion of clones with different Mycobacterium spp. rRNA genes suggested that such organisms comprised a significant fraction of microbes in the pool water (to >30%) and preferentially partition into aerosols (to >80%) relative to other waterborne bacteria present. The results of the study strongly validate aerosol partitioning as a mechanism for disease transfer in these environments. The results also show that culture protocols currently used by public health facilities and agencies are seriously inadequate for the detection and enumeration of potential pathogens.
Air Microbiology↗
A survey of genes in the Atlantic salmon (Salmo salar) as identified by expressed sequence tags.
We describe the construction and quality analysis of six cDNA libraries from the liver, ovary, testis, brain, spleen and muscle tissues of adult Atlantic salmon. The cDNA libraries were then screened with total cDNA probes to catalogue clones representing the abundant and rare mRNA populations in each tissue. Subsequently, the 5'-terminal DNA sequences of 1152 cDNA clones, composed of 96 clones from each of the abundant and rare mRNA populations in the six tissues, were determined. Bioinformatic analysis revealed that 510 (50%) of the salmon expressed sequence tags (ESTs) of sufficient length showed significant homology to previously identified genes from salmonid and other species, while 517 (50%) of salmon ESTs were unidentified or novel. After accounting for multi-EST redundancy, the 510 identified ESTs provided DNA sequence markers for 178 salmon genes which are listed in terms of tissue of origin and mRNA abundance class.
Animals↗
Microarray-based survey of repetitive genomic sequences in Vicia spp.
A modified DNA microarray-based technique was devised for preliminary screening of short fragment genomic DNA libraries from three Vicia species (V. melanops, V. narbonensis, and V. sativa) to isolate representative highly abundant DNA sequences that show different distribution patterns among related legume species. The microarrays were sequentially hybridized with labeled genomic DNAs of thirteen Vicia and seven other Fabaceae species and scored for hybridization signals of individual clones. The clones were then assigned to one of the following groups characterized by hybridization to: (1) all tested species, (2) most of the Vicia and Pisum species, (3) only a few Vicia species, and (4) preferentially a single Vicia species. Several clones from each group, 65 in total, were sequenced. All Group I clones were identified as rDNA genes or fragments of chloroplast genome, whereas the majority of Group II clones showed significant homologies to retroelement sequences. Clones in Groups III and IV contained novel dispersed repeats with copy numbers 10(2)-10(6)/1C and two genus-specific tandem repeats. One of these belongs to the VicTR-B repeat family, and the other clone (S12) contains an amplified portion of the rDNA intergenic spacer. In situ hybridization using V. sativa metaphase chromosomes revealed the presence of the S12 sequences not only within rDNA genes, but also at several additional loci. The newly identified repeats, as well as the retroelement-like sequences, were characterized with respect to their abundance within individual genomes. Correlations between the repeat distributions and the current taxonomic classification of these species are discussed.
Blotting, Southern↗
Student evaluation of courses: determining the reliability and validity of three survey instruments.
The purpose of this study was to determine the reliability and validity of three instruments designed for student evaluation of courses: the Course, Library, and Computer (CLC); the Clinical (CLIN); and the Nursing Skills Lab (NSL) evaluation tools. Using data from 294 anonymous associate degree nursing students, internal consistency and reliability were demonstrated by the high alpha coefficients ranging from .85 to .94. An exploratory principal component analysis, with the varimax rotation, provided an estimate of construct validity.
Adult↗
Is reading part of the lifelong learning process for RNs?
A 1988 national survey of 1,406 Registered Nurses (RNs) was conducted at 44 sites in 25 states and the District of Columbia to determine what type of occupation-related and general literature they read, what time they devote to reading, their purposes for reading, and the uses of the materials they read. The survey found that nurses are reading. They read, purchase, and subscribe to significantly more general than professional literature. They report that libraries are accessible, but they use them on a limited basis. The RNs also responded that their supervisors do not to support them in their pursuit of reading. Clinical journals, clinical books, and health-related newsletters are the top nursing materials read, newspapers and fiction books the most popular general literature.
Adult↗
Drug information libraries on the Internet.
UNLABELLED: In the past several years there has been a dramatic proliferation of drug-related sites on the Internet. This article reviews the information found at selected Internet drug information libraries, and comments on its accuracy and implications. Drug-related sites were found by initially performing an Internet search on "psychoactive drugs" and then exploring links among the sites identified. Sites were chosen on the basis of comprehensiveness of information and positive or tolerant attitude toward drug use. While all classes of drugs are discussed at these sites, the primary foci of interest are synthetic and naturally occurring hallucinogens. Many of the biological materials discussed are legal and readily available. Information surveyed at these sites was largely accurate regarding the effects of various substances and biological sources of psychoactive compounds. CONCLUSIONS: Internet drug information libraries contain large amounts of information about a wide variety of drugs, including previously little-known biological sources of hallucinogens. The availability of this information could have significant effects on patterns of drug use.
Information Services↗
Tissue Molecular Anatomy Project (TMAP): an expression database for comparative cancer proteomics.
By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to report the initial results of our survey across different tissues and explore the extent to which this comparative approach may help uncover panels of potential biomarkers of tumorigenesis which would warrant further examination in the laboratory.
Databases, Protein↗
Medical researchers and clinicians as seekers of information: the Nigeria case study.
Medical researchers and clinicians drawn from a random sample of health professionals in Nigeria were surveyed by means of a questionnaire to determine their sources of information. Sources of information were categorized into library related sources and non-library related sources in the first instance and responses analyzed in order to demonstrate which of the two categories of sources are utilized most by medical researchers or clinicians. A two-factor ANOVA analysis with repeated measures on one factor showed that researchers make significantly more use of library related sources of information than clinicians. A further grouping of source into bibliographic sources and AV materials and their subsequent analysis by use of a chi-square test, showed that clinicians make significantly greater use of AV materials than researchers, while the reverse is the case for bibliographic sources.
Developing Countries↗
A quest for questionnaires.
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Evidence-Based Medicine↗
A little e-learning can go a long way in transforming a traditional print-based distance learning course: a case study at the UK's Open University.
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Curriculum↗
The UK's SMARTAL Project: St MARTin's College health students Access to Learning resources whilst on placement.
BACKGROUND: St Martin's College pre-registration health students spend up to half their course on periods of placement, during which times they need to be able to access learning resources. The project aimed to replicate the Health and Education Northumbria Students Access to Learning resources (HENSAL) project. OBJECTIVE: To look at patterns of use and access to learning resources for health students on placement. METHODS: Questionnaires were distributed to students who had recently returned from a placement. Five Practice Placement Facilitators were interviewed to gain their views. RESULTS: There was a 60% return rate (n = 268) for the questionnaire. Most students thought access to learning resources was important yet these were not always available and varied according to placement type. Students still made regular use of the College library while they were on placement but had some difficulties. Students used electronic resources, tending to access them from home. CONCLUSIONS: The results indicated that there were issues which needed to be addressed by the College's Faculty of Health and Social Care, Library and IT services, the local NHS Trust libraries and the local Strategic Health Authority to improve access to and use of learning resources. The findings proved to be very similar to those of the HENSAL project.
Health Occupations↗