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Analysis of soluble Entamoeba histolytica antigen using enzyme-L inked electroimmunotransfer blots.

Soluble antigens of ten strains of E. histolytica were studied by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked electroimmunotransfer blots (EITB). No relations of immune replicas to virulence, geographical origin and method of cultivation (xenic or axenic culture) were found. Antigens of all ten strains tested precipitated with anti-E. histolytica human serum in the area of 30-43 kD. Antigen of HK-9 strain created in this area a characteristic pattern with all sera containing the specific anti-E. histolytica antibodies and, therefore, EITB can be used for excluding false positive results in ELISA.

Animals↗

[The phagocytosis activity of India ink in the isolated rat liver perfused with piracetam].

The activity of the reticulohistiocytic system of the isolated perfused liver was measured under the influence of piracetam (Nootrop). Also the parameters of liver function under the influence of piracetam were determined. If the energetical properties were not optimal it was shown that the phagocytic activity increased under the influence of piracetam. Also the parameters of liver cell function increased. Under optimal energetic properties there was no changing of the phagocytic activity but the liver cell parameters decreased.

Animals↗