[The prognostic value of in-vitro tests; a review of 30,000 in-vitro tests during the course of chemotherapy].
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By means of semipurified tetanus toxin for solid phase coating in an enzyme immunoassay (ELISA) for detection of specific IgG and IgM antibodies a detection limit of 0.02 IU per litre was achieved. The addition of serum from animals like horses or goats as inert protein to the dilution medium was omitted to prevent a displacement of human antibodies by antitetanus antibodies present in the animals sera. The specificity of the ELISA was demonstrated by inhibition experiments with soluble antigen and in an ELISA for detection of anti-tetanus toxin antibodies from mice immunized with the toxoid from the different purification steps.
GS inhibit both the function of spontaneously occurring helper and suppressor T cells, but the latter activity appears to be more sensitive. The process of induction of suppressor T cells by Concanavalin A is partly steroid-sensitive. Active ConA-induced suppressors appear to be more resistant to GS.
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