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PubMed1981

Pregnancy rate among beef heifers from timed insemination following synchronization with a progestin treatment.

Five trials were conducted with 958 virgin beef heifers to evaluate the effects of various breeding management methods on pregnancy rate at synchronized estrus and during a 45-day artificial insemination (AI) season. Synchronization was achieved with the progestin treatment regimen Syncro-Mate-B (SMB). Pregnancy rate at first service and cumulative pregnancy rate were affected (P less than .01) by trial. Pregnancy rate at first service was 55% for all heifers on the SMB treatment regimen and 67% for nontreated control heifers (P less than .01). However, cumulative pregnancy rate during the 5-day synchronized period was higher (P less than .01) among treated heifers (54%) than among control heifers (21%). The pregnancy rate was 6% higher (P less than .06) after 27 days of breeding for treated heifers than for control heifers. Neither pregnancy rate at 21 days nor final pregnancy rate after a 45-day AI season was significantly affected by treatment. Pregnancy rate at first service among heifers undergoing the SMB treatment regimen and inseminated 12 hr after first detected in estrus was not significantly different from that among heifers inseminated once from 45 to 55 hr after implant removal or heifers inseminated twice at 48 and 60 hr after implant removal, regardless of occurrence of estrus.

Animals↗
PubMed1982

Influence of PMSG and time of artificial insemination on fertility of progestogen-treated sheep in confinement.

The need for pregnant mates' serum gonadotropin (PMSG) in breeding confined sheep by artificial insemination (AI) at progestogen-synchronized estrus was assessed in 152 adult crossbred ewes brought into season by a controlled light regimen. One-half of the ewes received 500 IU PMSG after intravaginal progestogen treatment; all ewes were inseminated either 54, 57 or 60 h after sponge removal or at 54 and again at 60 h. Based on progesterone determinations 18 d after AI, conception rates with single insemination 54, 57, or 60 h and double insemination at 54 and 60 h were 76, 72, 47 and 72%, respectively, among ewes receiving PMSG, compared to 17, 22, 47 and 43%, respectively, among ewes not give PMSG (P less than .01) Lambing rates were higher (P less than .01) with PMSG (67, 67, 37 and 61%) than without PMSG (11, 11, 26 and 33%). While there was only a small increase (.06 less than P less than .05) in litter size with PMSG, fecundity decreased (P less than .01) from 1.4 to .3 when PMSG was not used. These data indicate that, even with controlled lighting to induce estrous activity, additional stimulation of ovulation by PMSG at progestogen-synchronized estrus is necessary for normal fertility when confined sheep are bred by AI.

Animals↗
PubMed1982

Effect of acetylcholine, prostaglandin F2 alpha and estradiol on number of sperm in the reproductive tract of inseminated rabbits.

Acetylcholine, injected im into does immediately after natural mating, significantly increased the number of sperm recovered 1 h later from the vagina and cervices but not from the oviducts. Acetylcholine caused immediate increases in the frequency and amplitude of uterine contractions as measured by strain-gauge force transducers attached to the serosa and recorded by physiograph; contraction patterns returned to the preinjection pattern within 30 min. Administration of prostaglandin F2 alpha (.75 mg) at the time of insemination increased sperm numbers significantly at 2.5 h in the oviducts, uterus and cervices; .15 or 3.75 mg caused relatively little increase in sperm numbers. Prostaglandin F2 alpha increased the number and strength of uterine contractions for more than 30 min. Estradiol injected 1 h before artificial insemination increased the number of sperm recovered from the oviducts, uterus, cervices and vagina at 2.5 h after insemination. Estradiol caused little if any detectable increase in the frequency or strength of uterine contractions during the period of increased retention and transport of sperm. When does were inseminated with low numbers of sperm (86,000), prostaglandin F2 alpha and phenylephrine, used for comparison, each increased the ovum fertilization rate (22% for control does; 76 and 73%, respectively, for prostaglandin F2 alpha- or phenylephrine-treated does).

Acetylcholine↗
PubMed1983

Seasonal effects of PMSG and number of inseminations on fertility of progestogen-treated sheep.

The influence of pregnant mares' serum gonadotropin (PMSG) on reproductive performance of sheep bred by artificial insemination (AI) was studied in the anestrous and estrous seasons. Ewes were treated with progestogen-impregnated intravaginal sponges in June and October. One-half of the ewes in each trial received PMSG at sponge removal and were inseminated 55 h after sponge removal. One-half of the ewes in each treatment group were reinseminated 5 h later. Conception rates in June and October were 82 and 87% with PMSG and 18 and 48% without PMSG, respectively. The corresponding lambing rates were 60 and 74% with PMSG and 10 and 26% without PMSG. Litter size was unaffected by season or PMSG use. Embryonic mortality estimated over both trials was 22% after the first 2 wk of pregnancy with PMSG, but was 44% when PMSG treatment was omitted. Two inseminations were not superior to one. These data indicate that irrespective of season or double insemination, PMSG improves reproductive performance of ewes bred by AI at progestogen-synchronized estrus.

Animals↗
PubMed1989

Intrauterine insemination with washed sperm.

Intrauterine insemination with washed semen has been carried out in our department since the beginning of 1986. Couples are examined carefully according to the principles of WHO before entering the program. The cycles of those couples treated by intrauterine insemination were monitored. Oestradiol values showed the highest levels on days 13 and 14, such as the size of folliculi, and serum progesterone level increased on days 14 and 15 ovulatory cycle. Insemination was performed with washed sperm after cleansing from it different albumin components and bacteria; the insemination was carried out in two groups. Good results were achieved in cases where the poor quality or hostility of the cervical mucus was the cause of infertility. The treatment can be successful if the spermiogram does not show severe deviation. No success can be expected in severe deviation of the spermiogram.

Estradiol↗
PubMed1979

Pregnancy rate in dairy cows inseminated on the basis of electronic probe measurements.

A new vaginal probe with two pairs of parallel electrodes 90 degrees apart was designed with a switching arrangement so that electrical resistance (ohms) of the dorsal and the ventral areas of the anterior vagina could be monitored separately during the estrous cycle. Three inseminators probed cows during February--April, 1977, in nine herds in stanchion barn housing. Cows not seen in estrus by 40 to 60 dyas after calving were probed every 3rd day. At each dairyman's option normal cows with a low porbe reading were inseminated. Controls consisted of cows seen in estrus by the dairyman and reported for insemination in the routine manner. The diagnosed pregnancy rate for 86 first service controls was 49% versus 52% for 58 cows not seen in estrus, but identified by low probe readings. Cows varied in electrical resistance at breeding, but the fact that pregnancy rates were equivalent in the two groups is interpreted to mean that the probe was as effective in identifying cows suitable for insemination as was visual observation of estrus by dairymen.

Animals↗
PubMed1980

Effects of two simulated semen culling programs on predicted fertility in an artificially inseminated cow population.

Two semen culling programs designed to improve fertility of semen used in an artificial insemination program were compared. First inseminations and return service data on cows inseminated with semen from 21 Holstein bulls during 1 yr were studied. The culling of less fertile ejaculates within bulls, based on any available semen quality test or combination of tests, would bring about less than a 1% increase in nonreturn rate if 40 to 50% of ejaculates were called. If all semen from the 19% low fertility bulls were culled, this would entail culling 22% of the semen used to inseminate the population and would achieve an improvement of 1% in overall fertility. This could be done with no loss in predicted difference for milk yield of bulls used. Culling of bulls is a functionally simpler and less expensive program than is testing and culling ejaculates within bulls, but this requires that fertility of bulls be known and that it will not change substantially from predicted.

Animals↗
PubMed1983

Radiographic method for evaluation of bovine artificial inseminating technique.

Radiography was used to visualize the interior of excised bovine reproductive tracts during artificial insemination. Reproductive tracts were placed in a device that simulated the anatomical position of the reproductive organs in vivo. Air was introduced into the uterus to provide differential radiopacity between the uterine lumen and the surrounding soft tissue. Egg yolk extender was saturated with lead acetate to provide radiopacity, thus enabling the inseminate to be visualized following deposition. This radiographic technique enables the insemination syringe tip and the distribution of the inseminate within the reproductive tract to be visualized clearly without dissection.

Animals↗
PubMed1985

Systematic environmental, direct, and service sire effects on conception rate in artificially inseminated Holstein cows.

Result of insemination was verified for 329,314 artificial inseminations by 882 service sires to 97,245 Holstein cows in 1,075 herds between May 1970 and December 1983. Estimates of systematic environmental and genetic components of cow and service sire components of conception rate were obtained preliminary to development of a fertility monitoring system. Conception rate was 2.9% higher in stanchion than loose housed herds and 2.3% higher in grade than registered cows. Fall months were superior to winter months, the greatest difference being 6.1% between October and January. Conception rate increased with herd milk production, decreased with both increased cow age, and increased herd size in mature and old cows. Regions and inseminators within regions were highly variable. Conception may be influenced by semen price; however, week day of insemination and duration of semen storage had no effect. Conception rate decreased for semen by bulls 8 yr and older, was lowest for semen harvested in June, but no season of collection effect was detected. No genetic trends for cow and service sire conception rate were found; heritability and repeatability were .08 and .06, respectively. Genetic correlations between cow and service sire conception rate and these components with first lactation production and semen output measures were all near zero. Therefore, the relationship between sire fertility and daughter fertility is near zero.

Animals↗
PubMed1975

The effect of time of day of insemination and oviposition on the fertility of turkey hens.

Turkey hens were inseminated at five different times of the day to determine the effect of time of day of insemination and the stage of egg formation on fertility. Results showed that fertility resulting from the 6 p.m. insemination was significantly greater than at 8 a.m., 10 a.m., 1 p.m. or 3 p.m. Conversely, fertility was significantly lower at 1 p.m. than at the other four times. Fertility was significantly lower if hens were inseminated during the last 10 hours that the egg was in the uterus and during the approximate time of ovulation than during the other times of egg development.

Animals↗
PubMed1976

Fertility of frozen chicken semen after intravaginal and intrauterine inseminations using various concentrations and equilibration times of dimethylsulfoxide and a range of freezing and thawing rates.

The use of dimethyl sulfoxide (DMSO) as a cryoprotective agent for freezing chicken semen was examined with respect to intravaginal (I.V.) and intrauterine(I.U.) inseminations. Semen diluted in DMSO was subjected to various freeze rates, equilibration times and thawing rates. Various concentrations of DMSO were examined as was the effect of diluting DMSO with phosphate buffer and Lake's solution. There was no significant (p greater than 0.05) effect of rate of freeze (ranging from 1.3 degrees C to 7.2 degrees C. per min) on fertility. However, rapid thawing in an ice bath resulted in a longer (p less than 0.05) duration of fertility than thawing at 1 degree C. per min., but had no effect on percent fertility during duration or percent hens fertile. Equilibration time and concentration of DMSO had no significant (p greater than 0.05) effects on fertility. There was no interaction between insemination route and Lake's solution vs. phosphate buffer with respect to duration of fertility. It was concluded that intrauterine (I.U.) insemination resulted in superior fertility to intravaginal (I.V.) inseminations with semen frozen in DMSO.

Animals↗
PubMed1980

Artificial insemination of individually caged broiler breeders. 1. Reproductive performance of males in relation to age and strain of females.

Studies were conducted to evaluate the reproductive capacity of male broiler breeders used for artificial insemination over an extended period and mated to females of six strains. Biweekly determinations were made of semen volume, semen concentration, and spermatozoal motility for each of the 47 males caged individually from their 39th to 63rd week of age. The percent fertility, duration of fertility, and percent hatchability were determined at five-week intervals (periods) using three young and three force-molted broiler female strains. With young female strains, the mean percent fertility for a 7-day collection of eggs following a single insemination (1 to 7 days) ranged from 95.7 to 81.1 over six consecutive periods. The mean duration of fertility (days) and the mean percent hatchability of fertile eggs was 13.3 and 13.0, and 92.5 and 84.9 respectively, for the first two periods. With force-molted hens, the mean percent fertility (1 to 7 days) ranged between 97.2 and 86.0 over the first three periods, and the mean duration of fertility and the mean percent hatchability was 13.2 and 12.8, and 91.5 and 84.1, respectively, for the first two periods. Fertility of eggs collected for 10 days following a single insemination was slightly but consistently lower than fertility over 7 days for both female groups. Significant differences among males and between periods existed for each semen trait. The effects of male, young female strain and period on fertility and duration of fertility were significant. Hatchability was significantly affected by period only. No male by female strain interaction existed for percent fertility or hatchability. A significant male by period interaction existed for percent fertility, semen volume and concentration. The results obtained are considered to support the feasibility of maintaining broiler breeders in cages and the use of artificial insemination (AI) to produce broiler hatching eggs.

Age Factors↗
PubMed1988

Influence of storage container for frozen-thawed chicken semen and frequency of insemination on fertility and its duration.

Pooled semen was diluted with Lake's solution containing 10.8% (v/v) glycerol. Aliquots of 1.2 mL and .5 mL were transferred to 1.8-mL capacity plastic vials and .5-mL capacity plastic straws, respectively. Semen was processed at 5 C, frozen by cooling at a rate of 3 C/min to -35 C, then plunged in liquid nitrogen. Samples were thawed at 5 C, diluted stepwise, centrifuged, and resuspended with nonglycerolized diluent at 5 C. Fertility and its duration were measured after single or double (insemination on 2 consecutive days) insemination. No significant differences were found in fertility percentages between semen samples frozen and stored in the two types of containers. However, fertility percentages were significantly higher for eggs laid on Days 2 to 5 after double insemination than for eggs laid after single insemination. No such differences were noted in the fertility of eggs laid on Days 2 to 7. Duration of fertility was significantly longer for semen frozen in plastic vials than for semen frozen in straws.

Animals↗
PubMed1991

Comparison of procedures for collecting semen from ganders and inseminating geese.

Experiments were conducted to compare management of ganders and semen collection procedures with respect to semen and sperm yield, and two frequencies of artificial insemination were tested with respect to fertility. Housing ganders in groups, singly, or singly with the introduction of a female just before collection was the rank order of housing system from least to most successful collection of ejaculates. There were no significant differences among types of housing with respect to semen volume, but ejaculates from ganders housed singly had the greatest (P less than .05) spermatozoal concentrations. Ejaculates collected with an artificial vagina were of greater (P less than .05) volume and total spermatozoal yield but not spermatozoal concentration than those collected by aspiration. Interactions between collector and method were observed for spermatozoal traits. Geese inseminated on 2 consecutive days/wk showed greater (P less than .01) fertility than those inseminated once per week. Therefore, collection of semen with an artificial vagina from ganders housed singly, with insemination weekly but on consecutive days, should result in successful reproduction of geese.

Animals↗
PubMed1984

Conditions for increased insemination rates in caged Aedes triseriatus (Diptera: Culicidae) reared from field-collected eggs.

Primarily in order to facilitate future studies on venereal transmission of La Crosse encephalitis virus by Aedes triseriatus, tests were conducted to identify conditions that maximize insemination rates in caged populations of Ae. triseriatus derived from field-collected eggs. All tests were conducted at 21 degrees C +/- 1 degree C, which is approximately the mean temperature during July and August in southwestern Wisconsin where La Crosse virus is endemic. Non-bloodfed females exposed on their 5th through 8th day of adult life showed little sexual receptivity to either 5- to 6-day-old males or 8- to 9-day-old males. F1 females were no more receptive than their mothers had been at a similar age. Mean insemination rates did not exceed 32.6% in any of these age combinations at sex ratios ranging from 4 males: 1 female-1 male: 4 females. Mean insemination rates ranged as high as 89.0% when non-bloodfed females were exposed on their 9th through 12th day of adult life to 5- to 6-day-old males in a 4 male: 1 female ratio. In females given a bloodmeal on their 4th day of life and exposed on their 5th through 8th day to 5- to 6-day-old males, mean insemination rates ranged as high as 91.7% at a ratio of 2 males: 1 female.

Aedes↗
PubMed1999

[Efficacy of simultaneous use of cryopreserved semen, controlled ovarian hyperstimulation and intrauterine insemination in the treatment of sterility caused by hypergonadotropic azoospermia].

The purpose of the present study is to determine the efficacy of an artificial intrauterine insemination program with frozen donor sperm and controlled ovarian hyperstimulation as an alternative therapy for infertility cause by hypergonadotropic azoospermia. Two hundred forty three insemination cycles with frozen donor sperm were analyzed. Clomiphene citrate, pure FSH, recombinant FSH or human menopausal gonadotropins were utilized for ovulation induction; human corionic gonadotropin (hCG), 10,000 IU, was administered when one or more dominant follicles with diameter > or = 16 mm were present; intrauterine insemination was performed 36 hours after the hCG injection. The pregnancy rate per cycle was 19.9%, and the cumulative pregnancy rate was 59.3%. It is concluded that intrauterine insemination with frozen donor sperm and ovulation induction is a good alternative for male factor infertility with no available treatment.

Chorionic Gonadotropin↗
PubMed1975

Results of insemination of mares with fresh and frozen stallion semen.

Artificial insemination using deep-frozen semen was performed on 116 mares in 1973 using twelve ejaculates from eight stallions of the Hanoverian breed. Despite the fact that the quality of the semen used was poor, the majority of the mares inseminated were subfertile and some mares were inseminated during one oestrus only, 44% nevertheless conceived and gave birth to live foals. Of a small group of ten mares inseminated in the same year with fresh semen, seven conceived.

Animals↗
PubMed2001

[Role of capacitation in intrauterine insemination as a treatment of male infertility].

BACKGROUND: Although FIVET and ICSI efficacy and efficiency are continuously increasing, intrauterine insemination (IUI) is a very used technique for many different types of sterility. It is also used in male sterility for its cost/benefit positive rate. Pregnancies frequency obtained after IUI in male factor infertility cases, with or without ovarian stimulation, shows the value of this work. METHODS: We evaluated 149 insemination cycles in 34 couples with a male infertility diagnosis confirmed after at least 2 semen analysis, according to OMS criteria. All the 34 patients had at least one Fallopian tube open and some spontaneous ovulatory cycle to enter in this study. The patients without biochemical pregnancy signs after 6 intrauterine insemination cycles were induced to multiple ovulations through oral administration of 50 mg of Clomifene citrate from the 5th to the 9th cycle-day or through intramuscular administration of 75 I.U. of FSH from the 5th cycle-day. A luteal support with 200 mg of vaginal progesterone, 2 times for day, was reserved for all the patients with endometrium <8 mm or with low progesterone serum levels (<20 nmol/l). RESULTS: All patients made 149 IUI cycles, with a total medium pregnancy rate for cycle of 6.0% and for patient of 26.4%. We didn't observe statistically significant differences related to spermatozoa total number for ml in native sperm, in terms of pregnancy rate in men with 100-150 millions of spermatozoa. We observed a lower pregnancy rate in men with less than 5 millions moving spermatozoa. Under 1 million of total spermatozoa, pregnancy rate is very low (2.3%): it is established for higher values, obtaining the best results between 10 and 20 millions of total spermatozoa (10.5%) and a little reduction over 20 millions (8.3%). CONCLUSIONS: The evaluation of spermatozoa total number and also their motility does not present any prognostical significance as to the pregnancy rate in intrauterine insemination for male factor infertility.

Adult↗