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[Effects of Dipterocarpaceae on arbuscular mycorrhizal fungi].

An investigation was carried out on the colonization percentage, spore density, relative abundance, occurrence frequency, and species richness of arbuscular mycorrhizal (AM) fungi on 4 species of Dipterocarpaceae trees grown both in natural forests in Yunnan and Hainan Provinces and in greenhouse pots. The results showed that all dipterocarp species were able to form AM, the colonization rates ranged from 30.6% to 45.3%, 37% on average. Hopea hainanensis (Dipterocarpacea) seedlings without AM fungal colonization were cultivated in pots with soil collected from Dipterocarpacea rhizosphere, and harvested a year later. The colonization rate of the seedlings ranged from 10.6% to 20.3%, 14.2% on average, indicating the significant effect of host plants on AM fungi frequency. The relative abundance of Glomu, Acaulospora and Gigaspora also varied with host plants. It was concluded that the dominant AM fungi in the rhizospheric soil of dipterocarp plants were Acaulospora spp. and Glomus spp. Using the same species of Dipterocarpacea as host plants might promote the growth and development of AM fungi, and increase the AM diversity.

Ericales↗

[Resource and ecological distribution of ectomycorrhizal fungi under pine forests of Huangshan Mountain district].

Pinus massoniana and Pinus taiwanensis are the most common and important tree species in the Huangshan Mountain district, and ectomycorrhizae plays an important role in their forestation. Our investigations in 1998-2003 showed that under the pine forests of this district, there were 43 species of ectomycorrhizal fungi belonging to 10 families and 17 genera, of which, 43 were under Pinus massoniana forest, and 12 under Pinus taiwanensis forest. Only a few species were found under young Pinus massoniana forest, with the dominant of Pisolithus tinctorius (Pers.) Coken and Rhizopogon spp., but under mature Pinus massoniana forest, there were plentiful species, with the dominant of Russulaceae, Amanitaceae, Boletaceae and Canthurellaceae. The relationships between woody species and ectomycorrhizal fungi, and between fungi distribution and temperature, moisture and soil condition were discussed in this paper, which would benefit to the further studies on the effects of different ectomyrrhizal fungi to Pinus massoniana and Pinus taiwanensis forests.

China↗

[Hydrolytic activity yeast-like fungi strains isolated from Sulejów reservoir water].

We have investigated the hydrolytic activity of 21 species of yeast-like fungi from Candida genus and 1 species of yeast S. cerevisiae isolated from Sulej6w Reservoir water. The activity of hydrolytic enzymes of the studied fungi was different than that of standard strains and also of fungi isolated from patients. This difference demonstrates the capability of the studied fungi to adapt to various environmental conditions.

Candida↗

[Contamination of dairy products by yeasts-like fungi].

Evaluation of the degree of contamination of diary products with potentially pathogenic fungal strains. Materials for the study were: yoghurt, cottage cheese, homogenised cottage cheese, cream and buttermilk. Differentiation of species was based on their morphological features and biochemical properties (API 20 C bioMérieux). Among 300 studied diary products we have isolated 100 (33.0 +/- 2.72%) strains of yeast-like fungi. Diary products were contamined with 19 species of fungi from types: Candida, Geotrichum, Trichosporon, Saccharomyces and Rhodotorula. The highest percentage of fungi was seen in cream (95.7 +/- 4.23%), cottage cheese (87.9 +/- 5.68%) and kefir (60.0 +/- 15.5%). In other studied products the frequency ranged from 13.1 +/- 2.54% to 35.2 +/- 6.51%. Among the isolated fungi the following species prevailed: Geotrichum candidum (18.0 +/- 3.84%), Candida lusitaniae (15.0 +/- 3.57%) and C. inconspicua (11.0 +/- 3.13%).

Animals↗

[Study on anti-tumor effect of medicinal fungi Phellinus igniarius extracts].

OBJECTIVE: To study the inhibiting effect of medicinal fungi Phellinus igniarius extracts on S180 tumor and the immunoregulation effect on the S180-induced tumor mice. METHOD: S180 mice were orally given 100, 200, 400 mg x kg(-1) dosage of P. igniarius extracts, then the inhibition grow effect, spleen index, and thyme index were measured. RESULT: Medicinal fungi P. igniarius extracts can increase the spleen index and thyme index and the inhibiting tumor rate was 31.88%, 46.25%, 53.13%, respectively. Also, medicinal fungi P. igniarius extracts can prolong life in mice. CONCLUSION: The medicinal fungi P. igniarius extracts show obviously anti-tumor effect and immunoregulation effect.

Animals↗

Dermatophytes and keratinophilic fungi in cats and dogs.

During an 11 month period from January to November 1987, dermatophytes and keratinophilic fungi were isolated from 70 (75%) of 93 cats and 62 (36.9%) of 168 dogs. The most common isolate was Microsporum canis from 54 (58%) cats and 33 (19.6%) dogs, followed by Trichophyton mentagrophytes from 5 (5.4%) cats and 9 (5.3%) dogs, Aphanoascus fulvescens (4 cats and 9 dogs), M. gypseum (5 dogs), Tr. terrestre (3 dogs) Myceliophthora vellerea (2 dogs) and Chrysosporium indicum, Chr. merdarium and Chr. tropicum from cats. Alternaria, Scopulariopsis, Penicillium, Cladosporium, Aspergillus and Acremoniumspp., in descending frequency, were the most commonly found fungi in cats and dogs. The frequent infestation by the mite Tyrophagus putrescentiae on M. canis cultures suggest that mites may play a part in the carriage of fungi among animals. This mycophagous mite could disperse the fungi in animal hair by way of their fecal pellets during a transitory infestation. In this instance, the female mite can lay eggs and these could hatch on the animal's hair or in Petri dishes in which hair samples are cultured. In this event the future prospects of animal prophylaxis against dermatophytes could be made more effective by the regular use of acaricide agents on animal hair.

Animals↗

[Clinico-experimental study of a dried selective medium for isolating fungi in the genus Candida].

The trial of a newly developed dried selective medium for the isolation of fungi of the genus Candida, involving the inoculation of 103 museum yeast strains and 542 specimens of pathological material, has been carried out. The data obtained in this trial indicate that the proposed medium has advantages over wort agar and Sabouraud medium with antibiotics in the germination index, in the ability to ensure fungal growth after the inoculation of pathological material, and in selective properties. The medium ensures the intactness of the morpho-cultural, biochemical and serological properties of the fungi. The results of the trial recommend the newly developed preparation for laboratory practice for the isolation of yeast-like fungi from clinical material in the diagnosis of candidiasis, intestinal microflora disturbances, as well as for isolation of the fungi from various environmental objects.

Agar↗

Immunogenicity of fungi for the production of reaginic antibody in the rat.

Immunogenicity of culture filtrates of various air-born fungi with respect to reaginic antibody formation was tested in the rat. Animals immunized with two successive injections of culture filtrates of fungi with the aid of Bordetella pertussis vaccine as an adjuvant produced fairly high titers of reaginic antibodies of the IgE class. Little cross-reactivity was detected between reagins induced by different strains of fungi. The reaginic immunogenicity was only found in the culture fieltrate produced by the surface culture, in which large amounts of conidia were formed, but not in the filtrate obtained from the shaking culture, in which only mycelial components were growing. Therefore, it was suggested that allergens responsible for the reaginic antibody formation derive from the conidium but not from the mycelium. The results indicate that air-born fungi are potentially immunogenic for production of reaginic antibodies, and further suggest their causative roles in human atopic diseases.

Adjuvants, Immunologic↗

Influence of soil on wood-degradation and fruit body formation by parasites and saprophytes among wood-destroying basidiomycetous fungi.

Formation of soil mycelium enables the fungal mycelium to amend the nutrient-deficient wood substrate actively by enclosing macronutrients from soil. Supposition to formation of a soil mycelium proved the resistance of the fungus to competitive substrate microorganisms. This resistance is absent in pathogenic, but wide-spread in saprophytic wood-decay fungi. Consequently, production of soil mycelium was restricted to saprophytic fungi. Saprophytes of the white-rot type are superior to brown-cubical rot type fungi in forming soil mycelium and utilizing soil nutrients. However, rich soils stimulate a more limited degree of wood degradation and yield increase of basidiocarps in parasitic fungi, too, that fail to produce a soil mycelium. It is concluded that uptake of soil nutrients turns out in two different ways, via an actively absorbing soil mycelium and via passive nutrient diffusion from soil into the substrate wood.

Antibiosis↗

[Incidence among children of Candida yeast-like fungi].

The incidence of Candida fungi in healthy children and those suffering from noninfectious diseases was studied. In dealthy children--from the time of birth to 7 years--it constituted 39%, and in sick children treated with antibiotics--53%. Dependence of the frequency of Candida fungi state on age was revealed; there was also a correlation between the amount of the fungi and the presence of lesions on the oral mucosa. Candida fungi were also revealed in the washings from the environmental objects, on the pharyngeal mucosa and hands of the personnel, and in the samples of food for children.

Anti-Bacterial Agents↗

[Role of fungi belonging to the genus Candida in the etiology of nonspecific pulmonary diseases].

The sputum specimens from 1363 patients at the age of 16 to 65 years with nonspecific pulmonary diseases and the pleural exudate specimens from 325 patients were tested for fungi in 1989-1994. The etiological significance of Candida was stated at a concentration of > 10(5) GFU per 1 ml of the sputum. The identification was performed by the routine methods. An increase in the rate of the fungi isolation was studied in the time course of the observation: 15.3 +/- 1.9 per cent in 1989 and 31.6 +/- 3.4 per cent in 1994. The fungi were more frequently isolated from the patients with lung abscesses (38.0 +/- 4.1 per cent of the cases). In the patients with pyothorax the fungi were isolated from the pleural exudate specimens only in 6.8 +/- 1.4 per cent of the cases. The groups of risk of the susceptibility to Candida were revealed. They included patients at the age of 21 to 30 years and above 60. Out of 484 Candida isolates 80.7 per cent belonged to C. albicans, 4.2 per cent to C. tropicalis, 2.1 per cent to C. kefyr and 1.8 per cent to C. krusei. The isolates of C. parapsilosis, C. guillermoudii, C. utilis and C. brumptii were rate. The isolates were highly susceptible to nystatin (91,8 per cent) and lowly susceptible to levorin (35.4 per cent), amphoglucamine (24.7 per cent) and ketokonazol (16.8 per cent).

Adolescent↗

Characterization of pathogenic fungi genomes using pulsed field gel electrophoresis.

Pulsed field gel electrophoresis (PFGE) has been firstly introduced in characterization of the pathogenic fungi Penicillium marneffei and Exophiala dermatitidis genomes. The numbers and sizes of their chromosomes have been detected. Polymorphism was identified on the smallest chromosome of E. dermatitidis. The result shows that PFGE for characterization of large molecular DNA pathogenic fungi is very suitable, it is more simple and more efficacy. The result also shows the diversity of pathogenic fungi is relative common even in rare occurred pathogenic fungi such as E. dermatitidis.

DNA, Fungal↗

Keratinophilic fungi isolated from children's sandpits in the Nablus area, West Bank of Jordan.

The keratinophilic fungi of 29 sandpits from kindergarten schools and public parks in the city of Nablus was analysed to evaluate their role in the epidemiology of diseases caused by these fungi. Seventy two species were recovered 28 of which were common to both kindergartens and public parks sandpits. High percentage (57.4%) of fungal isolates found had been identified as the causes of various types of mycoses. Eight species of dermatophytes and closely related fungi were recovered, of which the followings were the most commonly found species in sandpits: Chrysosporium keratinophilum (20.7%), Microsporum gypseum (17.2%), Trichophyton mentagrophytes (6.9%), and C. evolceanui (6.9%).

Alternaria↗

Detection of fungi in cerebrospinal fluid.

With the exception of Cryptococcus neoformans, fungi are rarely detected in cerebrospinal fluid obtained from patients having or suspected of having fungal meningitis. A review of the literature reveals that several fungi have been either isolated, observed, or both in cerebrospinal fluid specimens. These fungi include Acremonium species, Aspergillus amstelodami, A. flavus, A. fumigatus, A. oryzae, A. terreus, Blastomyces dermatitidis, Candida albicans, C. tropicalis, C. viswanathii, Coccidioides immitis, Cryptococcus albidus, C. neoformans, Histoplasma capsulatum, Paecilomyces variotii, Paracoccidioides brasiliensis, Pseudallescheria boydii, Schizophyllum species, and Sporothrix schenckii. Laboratory findings for cerebrospinal fluid specimens obtained from such patients and clinical presentations are summarized.

Aspergillus↗

Biological control of nematode parasites in cattle with nematode-trapping fungi: a survey of Danish studies.

In Denmark two series of experiments have been performed to study the interactions between larvae of bovine gastrointestinal nematode parasites and nematode-trapping fungi. For practical reasons we were interested in the possibility of depositing nematode-trapping fungi in cattle faeces after passage through the gastrointestinal tract. In the first series, laboratory tests with the fungus Arthrobotrys oligospora showed that motile free-living larvae of a wide range of animal-parasitic nematodes and some soil-living nematodes effectively induce the formation of traps. Larvae of all parasitic nematodes are rapidly captured in these traps. The induction of nets was influenced by temperature, number of larvae, atmosphere, light, and media composition. Captured first- and second-stage larvae were quickly penetrated and killed while third stage larvae were killed slowly, perhaps because they are partially protected by an outer dead sheath. Laboratory and field studies showed that when A. oligospora material was directly mixed into dung a significant reduction in the number of infective parasite larvae in the dung and surrounding herbage occurs. This reduction was also reflected in the acquired worm burden of calves grazing on fungal treated pasture. However, the A. oligospora strain studied in the above mentioned experiments did not survive passage through the alimentary tract of cattle. This prompted us to start a second series of experiments to isolate fungi that could survive gut passage of cattle. Different soil and compost samples were screened by an in vitro stress selection technique. This simulated certain important stress factors which occur during passage through the alimentary tract of ruminants. Rumen exposure was found to be a major limiting factor, but some Arthrobotrys and Duddingtonia strains survived submersion in rumen fluid. In a subsequent in vivo experiment, some of these survivors were fed to calves, and it was hereby demonstrated that isolates of both genera, i.e. Arthrobotrys and Duddingtonia, were able to survive passage through calves and significantly reduce the number of developing preparasitic larvae in dung of fungal treated calves. In a controlled field experiment, isolates of Duddingtonia reduced the level of infective third-stage larvae in herbage by 74-85%.

Animals↗

Identification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis.

The nucleotide sequences of the D1/D2 domains of large subunit (26S) ribosomal DNA for 76 strains of 46 species of pathogenic dematiaceous fungi and related taxa were determined. Intra-species sequence diversity of medically important dematiaceous fungi including Phialophora verrucosa, Fonsecaea pedrosoi, Fonsecaea compacta, Cladophialophora carrionii, Cladophialophora bantiana, Exophiala dermatitidis, Exophiala jeanselmei, Exophiala spinifera, Exophiala moniliae, and Hortaea werneckii were extremely small; as few as 0 changes were detected in C. bantiana, Fonsecaea and Exophiala species, 1 bp in C. carrionii and H. werneckii, and 2 bp in P. verrucosa. Inter-species nucleotide diversity between most species was higher. These data suggested that the D1/D2 domain is sufficiently variable for identification of pathogenic dematiaceous fungi and relevant species. The phylogenetic trees constructed from the sequence data revealed that most human pathogenic species formed a single cluster and that Cladosporium and Phialophora species were distributed polyphyletically into several clusters.

Ascomycota↗

Marine fungi from Mira river salt marsh in Portugal.

The occurrence of fungi in the Mira salt marsh, Portugal was investigated for 12 months. Baits of Spartina maritima stems were exposed to permanent or temporary submersion at the upper and lower limits of the intertidal zone. The baits were observed for fruit bodies and spores directly and after incubation in moist chambers. Twenty six marine species were identified (17 Ascomycota, two Basidiomycota and seven mitosporic fungi). Twenty four are new records for Portugal. Nia globospora Barata and Basílio was published as a new species. Species were characterized with respect to frequency of occurrence, colonization capability and substrate succession. The diversity and similarity indexes of the fungi under different conditions were determined.

Ascomycota↗

The ecology of keratinophilic fungi at different depths of soil.

The influence of depth and humus content of soil, on the presence of keratinophilic fungi in 4 layers (1-10 cm, 11-25 cm, 26-40 cm, 41-55 cm) of carbonate meadow, chernozem carbonate meadow and carbonate alluvial soils was studied. Different keratinophilic fungi were found not only in individual soils but also in different layers of the same soil. The greatest number and quantity occurred in soil with highest humus content. These fungi were present in greatest amount in the superficial layers (1-10 cm and 11-25 cm) of all soils investigated.

Ascomycota↗