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At least 685 records · Page 38Linked to original sources
PubMed1980

[Isolation, purification and some properties of tyrosine-phenol-lyase from Citrobacter freundii cells].

A new procedure for isolation of tyrosine-phenol-lyase from the cells of C. freundii strain 62 allowing to obtain a highly purified enzyme with a high yield at a reduced time expenditure has been developed. The procedure described differs from the well-known method for isolation of the enzyme from the cells of Escherichia intermedia and Erwinia herbicola. Some properties of the enzyme from C. freundii 62, e.g. stability, dependence of the enzyme activity on some mono- and bivalent cations and pH- and temperature dependences of the enzyme have been studied. It was shown that the enzyme is activated by NH4+, K+, Na+ and is inhibited by Ca2+, Cu2+ and Mg2+. The enzyme loses up to 50% of its activity upon storage in glycerol with 2-mercaptoethanol during 1,5 months at -18 degrees.

Cations, Divalent↗
PubMed1995

[Biochemical indicators of Citrobacter sedlakii].

The authors describe eight strains identified biochemically as a new species of C. sedlakii in clinical material, surface water and the cutting surface of a melon. The majority of strains was isolated in the Czech and Slovak Republic.

Citrobacter↗
PubMed1975

[Citrobacter persistence in the intestinal tract of guinea pigs depending on the microecological status].

Gnotobiological studies were conducted on germ-free guinea pigs. Association of the animals with the citrobacteria 5396/38 strain led to the reproduction of these bacteria in numbers exceeding the sum total number of all the representatives of the normal microbial flora in the conventional animals. Association lasting for a period of 46 days caused no antibody formation in the blood serum of these animals in difference to conventional guinea pigs immunized parenterally. Conventionalization of the monoflora guinea pigs served as the beginning of population of the intestinal tract by other microbial representatives which replaced the first culture. Biochemical properties of the citrobacteria strains isolated from the monoflora strains were identical to the properties of the initial culture. After the monoflora animals were let out of the isolation cage the citrobacteria strains displayed changes of some differential-diagnostic signs. Passage through the macroorganism of the citrobacteria stain proved to lead to the changes in the antigenic structure.

Animals↗