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PubMed2001

Communication ecology of webbing clothes moth: 1. Semiochemical-mediated location and suitability of larval habitat.

We tested two hypotheses: 1) that there is semiochemical-mediated attraction of male and female webbing clothes moth (WCM), Tineola bisselliella (Hum.) (Lepidoptera: Tineidae) to suitable larval habitat; and 2) that selection of optimal larval habitat has fitness consequences. In binary or ternary choice arena bioassay experiments that prevented WCM from contacting test stimuli, males and females were attracted to dried but untanned animal pelts (red squirrel, muskrat, beaver, coyote, red fox and bobcat) and preserved horseshoe crab but not to unprocessed sheep's wool, demonstrating semiochemical-based recognition of, and discrimination between, potential larval habitats. Selection of habitat has fitness consequences for ovipositing females, because significantly more male and female WCM completed development when the larval diet consisted of intact animal pelt (hide plus hair) rather than hide or hair alone. Equal attraction of male WCM to muskrat pelt volatiles in Porapak Q or solvent extracts of muskrat pelts indicated that volatile semiochemicals could be obtained by both methods.

Adaptation, Physiological↗

PubMed2001

Communication ecology of webbing clothes moth: 2. Identification of semiochemicals mediating attraction of adults to larval habitat.

Our objective was to identify the semiochemicals that mediate attraction of the webbing clothes moth (WCM), Tineola bisselliella (Lepidoptera: Tineidae), to suitable larval habitat. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analyses of Porapak Q-captured bioactive volatiles from horseshoe crab, and dried but untanned vertebrate pelts revealed numerous EAD-active volatiles. These volatiles were identified by comparative GC-mass spectrometry and GC-EAD analyses of natural and synthetic compounds. A blend of 28 synthetic candidate semiochemicals attracted both male and female WCM. Experiments deleting various components determined that saturated aldehydes--but not unsaturated aldehydes, saturated hydrocarbons, saturated alcohols, or ketones--were essential for blend attractiveness. A blend of nonanal, the single most attractive aldehyde, in combination with geranylacetone was more attractive to WCM than the 28-component blend or dried, untanned animal pelt. Selection of larval habitat resides more with male than female WCM, as indicated by stronger EAD responses from male than female antennae to habitat-derived semiochemicals, and more selective and early response to habitat cues by males than females. Exploitation of nonanal and geranylacetone as resource-derived semiochemicals by both adult WCM and its larval parasitoid, Apanteles carpatus, is an example of convergent semiochemical parsimony.

Age Factors↗

PubMed2006

Assessment of dermal exposure to benzene and toluene in shoe manufacturing by activated carbon cloth patches.

OBJECTIVES: The aim of this investigation was to use activated carbon cloth (ACC) patches to study the probability and extent of dermal exposure to benzene and toluene in a shoe factory. METHODS: Inhalation and dermal exposure loading were measured simultaneously in 70 subjects on multiple days resulting in 113 observations. Dermal exposure loading was assessed by ACC patches attached to likely exposed skin areas (e.g. the palm of the hand and abdomen). A control patch at the chest and an organic vapor monitor (OVM) were used to adjust the hand and abdomen patches for the contribution from the air through passive absorption of benzene and toluene on the ACC patches. Systemic exposure was assessed by quantification of unmetabolized benzene (UBz) and toluene (UTol) in urine. RESULTS: Mean air concentrations for the study population were 1.5 and 7.5 ppm for benzene and toluene, respectively. Iterative regression analyses between the control patch, OVM and the dermal patches showed that only a small proportion of the ACC patches at the hand had likely benzene (n = 4; mean 133 microg cm(-2) h(-1)) or toluene (n = 5; mean 256 microg cm(-2) h(-1)) contamination. Positive patches were exclusively observed among subjects performing the task of gluing. Significant dermal exposure loading to the abdomen was detected only for toluene (n = 2; mean 235 microg cm(-2) h(-1)). No relation was found between having a positive hand or abdomen ACC patch and UBz or UTol levels. In contrast a strong association was found between air levels of benzene (p = 0.0016) and toluene (p < 0.0001) and their respective urinary levels. CONCLUSIONS: ACC patches are shown to be a useful technique for quantifying the probability of dermal exposure to organic solvents and to provide estimates of the potential contribution of the dermal pathway to systemic exposure. Using ACC patches we show that dermal exposure to benzene and toluene in a shoe manufacturing factory is probably rare, and when it occurred exposures were relatively low and did not significantly contribute to systemic exposure.

Abdomen↗

PubMed1997

Nylon cloth macular amyloidosis.

In primary localized cutaneous amyloid, deposition of amyloid is confined to the skin without the involvement of any internal organs. Amyloid deposition in the skin is often scanty, and electron microscopy may be needed to confirm the presence of the typical amyloid fibrils. There have been several case reports of cutaneous amyloidosis associated with friction or rubbing of the skin. We report a case of primary localized cutaneous amyloid associated with the habitual use of a nylon cloth.

Adult↗

PubMed1975

Properties and specificity of a second metal chelator-sensitive proteinase in the keratinolytic larvae of the webbing clothes moth.

The properties of a second metal chelator-sensitive proteinase (metalloproteinase 2) from the larvae of the webbing clothes moth, Tineola bisselliella, have been studied. The pH optimum for casein digestion was 9-4 and the enzyme showed high stability between pH 8 and 11, but very poor stability at acid pH. The proteinase was inhibited by EDTA, but not by an EDTA-calcium complex. EDTA inhibition could be reversed by addition of a slight excess of calcium or zinc ions. The cleavage specificity of metalloproteinase 2 against the A and B chains of S-carboxymethyl insulin was almost identical to that found previously for metalloproteinase 1.

Animals↗

PubMed1983

Dibutyryl cAMP-inducible alkaline phosphatase in animal cell plasma membranes: fluorescence detection of mutant clones on polyester cloth.

We have developed a rapid screening assay that allows us to estimate the alkaline phosphatase content of mouse L-M cell colonies immobilized on polyester cloth. This permitted the identification and isolation of two mutant clones with increased constitutive alkaline phosphatase activity and six clones that fail to express this activity when treated with dibutyryl cyclic AMP. Both of the strains with increased constitutive activity have basal enzymatic activities that are 6- to 7-fold higher than the activity of the parental strain. The extents to which the cyclic nucleotide further induces alkaline phosphatase in these two strains are different, however, indicating that they represent two classes of mutants. Studies using amino acids and synthetic peptides as alkaline phosphatase inhibitors suggest that only one alkaline phosphatase isoenzyme predominates, in both the parental and the mutant cell lines, with or without induction by cyclic nucleotide. Comparison to mouse tissues indicates that our cell lines express an isozyme resembling that found in kidney and bone. The six clones that fail to express alkaline phosphatase activity when treated with dibutyryl cyclic AMP also have extremely low basal levels of the enzyme. All of these mutant strains continue to synthesize protein when treated with dibutyryl cyclic AMP and undergo growth cessation and morphological changes in the presence of this agent. Thus, the mutations all appear to affect factors specific to the expression of alkaline phosphatase activity rather than factors that affect general cellular responsiveness or permeability to dibutyryl cyclic AMP. The characterization of these strains may help elucidate mechanisms of eukaryotic membrane protein biogenesis, enzyme induction, and regulation of gene expression by cyclic nucleotides.

Alkaline Phosphatase↗

PubMed1990

The effect of differing types of cloth and of contamination by non-feed nitrogen on intestinal digestion estimates using porous synthetic-fibre bags in a cow.

The influence of free surface area and pore size of a synthetic-fibre bag, and flow of intestinal substances into the bag, on the intestinal digestion estimates by the nylon-bag method of dry matter (DM), neutral detergent fibre (NDF), nitrogen, NDF-N and feed 15N was studied using a non-lactating cow fed on hay supplemented with barley and oats at maintenance level. The bags containing 15N-labelled ground ryegrass (Lolium perenne), barley, barley straw or rapeseed (Brassica napus) straw were introduced into the duodenum through a T-shaped cannula and collected from the faeces. Also, the disappearance of N from the bag was related to the true intestinal N digestion by comparing it with the net loss between duodenum and faeces of 15N in ryegrass introduced into the duodenum in an aqueous suspension. It was noted that the bag cloth often significantly affected both disappearance values and the subsequent chemical composition of the residues. Re-analysing part of the data as a 2 x 2 factorial (free surface x pore size) suggested that free surface area was often more important than pore size as a determinant of both disappearance values and chemical composition. Lower apparent (Kjeldahl N) than true (15N) feed N disappearance from the bag suggested a notable proportion of non-feed N in the residues, especially with fibrous feeds with low N. With ryegrass, 15N net loss within the intestine was lower than 15N disappearance from the bags. It was concluded that disappearance of Kjeldahl N is an underestimate of feed N disappearance from bags, but may possibly be an overestimate of the true intestinal digestion of feed N.

Agriculture↗

PubMed1998

ASTM F739 method for testing the permeation resistance of protective clothing materials: critical analysis with proposed changes in procedure and test-cell design.

ASTM (American Society for Testing and Materials) Method F739-96 specifies a test-cell design and procedures for measuring the permeation resistance of chemical protective clothing. Among the specifications are open-loop collection stream flow rates of 0.050 to 0.150 L/min for a gaseous medium. At elevated temperatures the test must be maintained within 1 degree C of the set point. This article presents a critical analysis of the effect of the collection stream flow rate on the measured permeation rate and on the temperature uniformity within the test cell. Permeation tests were conducted on four polymeric glove materials with 44 solvents at 25 degrees C. Flow rates > 0.5 L/min were necessary to obtain accurate steady-state permeation rate (SSPR) values in 25 percent of the tests. At the lower flow rates the true SSPR typically was underestimated by a factor of two or less, but errors of up to 33-fold were observed. No clear relationship could be established between the need for a higher collection stream flow rate and either the vapor pressure or the permeation rate of the solvent, but test results suggest that poor mixing within the collection chamber was a contributing factor. Temperature gradients between the challenge and collection chambers and between the bottom and the top of the collection chamber increased with the water-bath temperature and the collection stream flow rate. Use of a test cell modified to permit deeper submersion reduced the gradients to < or = 0.5 degrees C. It is recommended that all SSPR measurements include verification of the adequacy of the collection stream flow rate. For testing at nonambient temperatures, the modified test cell described here could be used to ensure temperature uniformity throughout the cell.

Gloves, Protective↗

PubMed1992

Rocky Mountain "spotless" and "almost spotless" fever: a wolf in sheep's clothing.

In 10 (10.8%) of 93 laboratory-confirmed or probable cases of Rocky Mountain spotted fever seen at Duke University Medical Center from 1969 to 1991, illness without rash or fleeting or atypical skin eruptions were noted. Data from these 10 cases and 33 similar previously reported cases of Rocky Mountain "spotless" or "almost spotless" fever support the premise that human Rickettsia rickettsii infection has a broader spectrum than that indicated by its classic description. Sixty-one percent of patients with Rocky Mountain spotless or almost spotless fever have been men. Two-thirds have been black. Although in some cases the absence of rash may be due to the prompt institution of therapy with chloramphenicol or tetracycline, in others long delays in recognition of the disease and treatment occurred, resulting in the deaths of 53% of patients. On the basis of outcome in the cases reviewed, empirical administration of chloramphenicol or a tetracycline to selected patients is justifiable (including those patients with severe organ dysfunction), even if they have no rash, a transient rash, or a rash of unusual distribution. Indeed, without such therapy, patients with spotless or almost spotless fever may die. Recognizing such atypical cases is analogous to seeing a wolf in sheep's clothing--intuition, experience, knowledge, and a high index of suspicion are required.

Adolescent↗

PubMed1999

Needlestick injury in clothing industry workers and the risks of blood-borne infection.

This paper identifies the hazard of a hollow needle device used extensively in the clothing industry and assesses the risk of transmission for HIV, Hepatitis B and Hepatitis C. A substantial risk of transmission is suggested and measures have been advised for its control. Occupational Health Physicians are advised to be aware of hollow needles in other industrial processes and where risks of cross-infection exist, the same safety considerations should be applied as in clinical medicine and veterinary work to avoid needlestick injuries. Needle sharing must be avoided.

Blood-Borne Pathogens↗

PubMed1992

Disinfection of drinking water by using a novel electrochemical reactor employing carbon-cloth electrodes.

A novel electrochemical reactor employing carbon-cloth electrodes was constructed for disinfection of drinking water. Escherichia coli K-12 (10(2) cells per cm3) was sterilized when a cell suspension was passed through the reactor at a dilution rate of 6.0 h-1, and a potential of 0.7 V versus a saturated calomel electrode was applied to an electrode. The survival ratio increased with increasing dilution rate but was less than 0.1% at dilution rates of less than 6.0 h-1. Although the survival ratio increased with increasing cell concentration above 10(3) cells per cm3, the disinfection rate also increased. The disinfection rate was 6.0 x 10(2) cells per cm3 per h at a cell concentration of 10(2) cells per cm3. Continuous sterilization of E. coli cells was carried out for 24 h. Sterilization is based on an electrochemical reaction between the electrode and the cell which is mediated by intracellular coenzyme A. Sterilization of drinking water by using this reactor was successfully performed, demonstrating the potential of such a reactor for clean and efficient water purification.

Disinfection↗

PubMed1978

Results after mitral valve replacement with cloth-covered Starr-Edwards prostheses (models 6300, 6310/6320, and 6400).

The actuarial survival and thromboembolic rates for the three types of cloth-covered Starr-Edwards mitral prostheses, models 6300, 6310/6320, and 6400 followed 6, 5, and 2 years, respectively, were not significantly different throughout the years they were followed. The combined cumulative survival and thromboembolic proportion at 5 years for these prostheses were 71 and 66 per cent, respectively. The thromboembolic rates were not different in the following two groups: (a) 238 patients receiving anticoagulants, and (b) 52 patients who had discontinued or who were not receiving anticoagulants. Four patients thrombosed their mitral prostheses. Another 8 per cent had class 3 symptoms after operation, which were attributed to myopathic or restrictive left ventricular dysfunction or other valvular disease.

Adult↗

PubMed1978

Physiological and biophysical limits to work in the heat for clothed men and women.

Heat-acclimated, lightly clothed men and women (four of each) walked on a treadmill at 25% and 43% VO2 max, respectively, (M =194 W.m-2), under seven air temperatures (Ta) ranging from 36 to 52 degrees C. Each experiment involved 1 h of fixed and a 2nd h of progressively increasing ambient vapor pressure (Pa). The relative steady state of rectal temperature (Tre), mean skin temperature (Tsk), and heart rate (HR) reached in the 1st h were forced upward during the 2nd h by the rising Pa. The critical air vapor pressure (Pcrit) was identified by the Tre point of inflection for each Ta. One man did not fully reach steady state, but inflection could be determined for his physiological responses. The mean values of all points of inflection were calculated for Tre, Tsk, and HR. Significant sex difference in HR was found only by excluding the results of the one man. Tre and Tsk showed no significant difference between men and women. The coefficient for evaporative heat transfer (he), which could be derived using the Pcrit for the low Ta range, was 14.5 +/- 2.2 W.m-1 Torr-1.

Acclimatization↗

PubMed1979

Wind speed limits to work under hot environments for clothed men.

Four heat-acclimated clothed young adult men exercised (treadmill) at metabolic rate of 191 W.m-2 (27% VO2 max), under five air temperatures (Ta) between 36 and 53 degrees C and three wind velocities (v), 1, 2, and 4 m.s-1, for 2 h. The 2nd h of each experiment involved progressive increases in the ambient vapor pressure (Pa) to force an upward inflection of the rectal temperature (Tre). The Tre point of inflection identified the critical Pa (Pcrit) for each Ta. The average mean skin temperature (Tsk = 36 degrees C for all Pcrit. Straight-line isotherms for Tsk = 36 degrees C, which agreed with the negative regressions of the Pcrit on Ta, represented the limits of exposure for Ta less than or equal to 44 degrees C. The slope characteristics of the isotherms corresponded with skin wettedness (w) of 0.94, 0.71, and 0.58, respectively, for v of 1, 2, and 4 m.s-1. For Ta greater than 44 degrees C the limit line corresponded with steeper negative regressions indicating a lower w than for Ta less than or equal to 44 degrees C. Despite the increase in the ambient evaporative capacity due to the higher v the limit lines describing exposure limits were not significantly different either at Ta less than or equal to 44 degrees C for v of 2 and 4 m.s-1 or at Ta greater than 44 degrees C for all three v.

Acclimatization↗

PubMedDate not supplied

Atherosclerotic expansive remodeled plaques: a wolf in sheep's clothing.

Geometric arterial remodeling is an important determinant of luminal narrowing in atherosclerotic disease. Expansive remodeling retards while constrictive remodeling accelerates luminal narrowing by plaque formation. Cross-sectional as well as follow-up studies revealed that expansive remodeling is associated with adverse cardiovascular events and a vulnerable plaque phenotype. Although the relation between expansive remodeling and plaque vulnerability is associative rather than causal, expansively remodeled plaques should be considered as a wolf in sheep's clothes. Further understanding of the processes that regulate arterial remodeling and plaque rupture may lead to new strategies to responsibly manipulate these processes for the benefit of patient outcomes.

Coronary Artery Disease↗

PubMed2000

Control of Pythium root rot on hydroponically grown cucumbers with silver-coated cloth.

Silver-coated cloth (SCC) effectively controlled root rot that was caused by Pythium aphanidermatum in hydroponically grown cucumber plants. The presence of SCC in the hydroponic solution reduced the root rot from 100% to 10% 20 days after inoculation with zoospores of P. aphanidermatum. It was suggested that the inhibition of SCC was caused not only by the silver ion dissolved from SCC, but also by the metallic silver and silver compounds formed on the surface of the root.

Cucumis sativus↗

PubMed1977

An unusual form of late stenosis after aortic valve replacement with a cloth-covered Starr-Edwards prosthesis.

A 24-year-old man presented with late stenosis of a cloth-covered Starr-Edwards valve (model 2320). Fibrous ingrowth occurred at the inflow orifice of the valve and all three struts were adherent to the aortic wall, creating a "tunnel" form of obstruction. The stenotic valve was replaced with a porcine heterograft, and the small aortic root was enlarged with a Dacron patch. This form of late prosthetic valve stenosis should have been prevented by enlargement of the aortic root at the time of valve insertion and by long-term anticoagulant therapy.

Adult↗

PubMed2000

Improved method for shooting distance estimation. Part 1. Bullet holes in clothing items

An improved method for firing distance estimation on clothing is described. The novel part of the method includes transfer of total nitrite (nitrite ions and smokeless powder residues) from the target to an adhesive lifter. After the transfer, lead and copper deposits around the bullet entrance hole are visualized by rhodizonate and rubeanic acid, respectively. The Modified Greiss Test is carried out after alkaline hydrolysis of the smokeless powder residues on the adhesive lifter.

Journal Article↗