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PubMed1982

The effects of castration on size and appearance of Sharpey's fibers of rat femurs: a scanning electron microscopic and microradiographic study.

Areas of Sharpey fiber bone from the proximal posterior diaphyseal surfaces of anorganic control and castrate osteopenic rat femurs were examined with the scanning electron microscope (SEM). The diameter of Sharpey fiber bundle insertion sites from castratre bone was significantly greater (P less than 0.001) than that from control bone. The fiber bundle insertions appeared as small pits and projections in control bone and as large pits in castrate bones. Microradiographs of cross sections of the Sharpey fiber bone revealed a greater (P less than 0.05) area of outer, lamellated periosteal bone in the castrates than in the controls, suggesting an accelerated bone apposition rate along castrate osteopenic femoral periosteal surfaces.

Animals↗

PubMed2003

Reduction of flobufen in pig hepatocytes: effect of pig breed (domestic, wild) and castration.

Knowledge of the biotransformation processes of veterinary drugs and food supplements in food-producing animals is increasingly important. Residual levels of parent compounds or their metabolites in food of animal origin may differ with the breed, breeding conditions, and gender of animals. The nonsteroidal antiinflammatory drug flobufen, 4-(2',4'-difluorobiphenyl-4-yl)-2-methyl-4-oxobutanoic acid (racemic or its individual enantiomers) was used as a model to evaluate differences in activity, stereoselectivity, and stereospecificity of reductases in primary cultures of hepatocytes from intact male or castrated male domestic pigs (Sus scrofa domestica) or male wild pig (Sus scrofa scrofa). Time-dependent consumption of flobufen enantiomers and formation of dihydroflobufen (DHF) diastereoisomers as their principal metabolites in hepatocytes were measured using chiral HPLC. Flobufen reduction in hepatocytes from all three experimental groups of animals was stereoselective ((+)-R-flobufen was predominantly metabolized) and stereospecific (2R;4S-DHF and 2S;4S-DHF diastereoisomers were preferentially formed). Flobufen reductases activity in male domestic pigs was 30 times higher compared to castrated pigs. Flobufen reductases activity was similar in domestic and wild pigs. The stereospecificity and stereoselectivity of DHF production did not significantly differ with breed or castration of animal. Chiral inversion of flobufen enantiomers was also studied and differences between castrated and intact male pigs were seen.

Animals↗

PubMed1975

The effects of castration on song development in zebra finches (Poephila guttata).

It has been suggested that the learning processes which occur during the critical period for bird song development are dependent on adrogens. Castration of male zebra finches (Poephila guttata, Estrildidae) at ages 9-17 days did not prevent song development, and normal learning occurred after the time of castration. Thus the learning processes of song development occur in the absence of gonadal androgens. Castrates tended to develop song more slowly than normals, which is potentially attributable to a decreased rate of singing in the castrates, or to a direct effect of androgen on the learning processes.

Animals↗

PubMed1982

Regional variations in the testicular dependence of prolactin binding and its possible relationship to castration-induced involution in rat prostate gland.

Prolactin binding sites of ventral, lateral, and dorsal lobes of rat prostate were examined immunohistochemically 1, 2, 4, and 8 days after castration or sham operation. In sham-operated rats each lobe exhibited a distinct pattern of intracellular and intraluminal prolactin binding. A loss in prolactin binding from epithelial cells of ventral prostate, which was visualized postcastration, was quantitated with the aid of an image analyzer and then statistically evaluated. The proportion of ventral prostate epithelial cells devoid of prolactin binding increased from approximately 13% in sham-operated rats to approximately 29% 1 day after castration, and reached a peak level of about 71% 4 days postcastration. No loss of prolactin binding was evident in either lateral or dorsal prostate up to 8 days postcastration. Direct measurement of epithelial cell heights and subsequent statistical evaluation revealed similar regional differences in the rates and extent of prostate involution. Eight days after castration ventral prostate epithelial cell heights decreased by 56% whereas lateral and dorsal lobe epithelial cells heights decreased about 25% and 14%, respectively. The apparent relationship between testicular dependence of prostatic prolactin binding and castration-induced prostatic involution are discussed in terms of possible regional variations in the prolactin-androgen interplay in prostate.

Animals↗

PubMed1983

Elimination of stored material from the seminal vesicles of the guinea pig following castration.

Experiments were carried out to determine what factors are involved in the disappearance of stored secretion from the seminal vesicles of the guinea pig and in the concurrent decline in weight of these glands following castration. Seminal vesicles of sexually mature males were unilaterally ligated, and the males were either castrated or left testes intact for a 14-day period. At necropsy, the seminal vesicles were removed and weighed, and biopsies were collected for histological inspection. The weight of nonligated vesicles from castrated males declined to less than half that of ligated sides. In testes-intact males, the weight of ligated vesicles was nearly double that of nonligated vesicles. Epithelial cell heights remained unchanged during the study. The rapid decline in weight of the guinea pig seminal vesicles following castration was attributed to the elimination of stored secretion by way of the urethra rather than by absorption.

Absorption↗

PubMed1983

Effect of castration and steroid treatments on the activity of some hydrolytic enzymes in dog prostate.

Adult mongrel dogs were castrated and treated by intramuscular injections of 5 alpha-androstane-3 alpha,17 beta-diol (androstanediol) alone or in combination with estradiol in order to find convenient enzymatic markers of hormone action in prostate. The activities of 15 hydrolytic enzymes were determined. Arginine esterase, acid sulfatase, and acid phosphatase were found to be the most sensitive markers of testicular hormones since they were decreased 18-, 5- and 5-fold respectively after 1 month of castration. The enzyme activities returned to precastration levels after 2 weeks of injection of androstanediol to castrated animals. The effect of androstanediol on the majority of the remaining enzymes was small. In general, the activities obtained after androstanediol treatment in combination with estradiol were similar to those obtained with androstanediol alone. Finally, beta-glucuronidase and neutral sulfatase were increased after castration, a finding that suggests that these enzymes are constituents of stromal cells. These studies will provide a basis for future studies of hormone action in the dog prostate.

Acid Phosphatase↗

PubMed1984

Biochemical difference between ventral and dorsolateral prostates: effects of castration and administration of the antiandrogen 16 beta-ethyl-17 beta-hydroxy-4-oestren-3-one on SDS-electrophoretic patterns of subcellular proteins from rat prostates.

Protein species in the cytosol, postnuclear particulate, and nuclear fractions from the ventral and dorsolateral prostates of rats castrated or injected with antiandrogen, 16 beta-ethyl-17 beta-hydroxy-4- oestren -3-one ( EHOO ), were analyzed by SDS-polyacrylamide gel electrophoresis. The effects of EHOO injection on the contents of the most abundant species in all three fractions of ventral prostate were similar to the effects of castration, but the effects of EHOO and castration were only similar on the nuclear fraction in the dorsolateral prostate. The most sensitive species to castration in the three subcellular fractions of dorsolateral prostate were the same as those to EHOO injections; this was not the case for the cytosol and nuclear fractions in the ventral prostate.

Androgen Antagonists↗

PubMed1984

Single case report of prostate adenocarcinoma in a dog castrated three months previously. Morphological, biochemical, and endocrine determinations.

During the course of another investigation, three dogs had been castrated 3 months previously. Upon completion of the experiment, it was discovered that one dog presented a spontaneous prostatic adenocarcinoma of intraalveolar proliferative type at histology. Prostate weight of this dog before castration was estimated to be 22 g by tridimensional measurement at laparotomy and remained relatively constant (19 g) 3 months after castration. These results indicate that if regression had occurred in some cell populations (androgen-dependent) it was only partial and masked by growth of androgen-independent cells. Analysis of 12 individual steroids in peripheral blood and in prostatic tissue attested of a normal adrenal secretory activity. A series of 15 hydrolytic enzymes along with receptors for androgen, estrogen, and progesterone, were determined in prostatic tissue obtained at sacrifice. Enzymatic activities were those of typical epithelial cells, and most of them remained relatively high despite low levels of circulating testosterone. However, two markers of androgen action in dog prostate, acid phosphatase and arginine esterase, were significantly reduced. Receptor levels were similar to those of castrated animals. Thus, cancer cells had probably retained some androgen sensitivity.

Adenocarcinoma↗

PubMed1999

Feedback control of gonadotropins in Atlantic salmon, Salmo salar, male parr.I. Castration effects in rematuring and nonrematuring fish.

Sexual maturation of Atlantic salmon (Salmo salar) male parr is a seasonally recurrent "all or none" response; either a fish matures fully or it does not mature. To study whether gonadal feedback on gonadotropic hormones, GTH I and GTH II, is involved in the control of maturation, previously mature Atlantic salmon male parr were either sham-operated or castrated in spring. They were then sampled during the onset of gonadal growth (late June-early July) or shortly before the breeding season (late September). In autumn, sham-operated males separated into two groups: nonrematuring males with low pituitary and plasma levels of both GTH I and GTH II, and those rematuring with high levels of gonadotropins. Castrated males had low GTH I and GTH II plasma and pituitary levels, similar to those of the nonrematuring fish, suggesting positive feedback mechanisms, separating the sham-operated fish into low and high GTH level groups. In the summer, plasma GTH II was nondetectable in all fish. Pituitary GTH II content was lower in nonrematuring, than in rematuring males and was even lower in castrated fish. In contrast, castration increased pituitary and plasma levels of GTH I in the summer, suggesting a negative feedback at this reproductive stage. There were no significant differences in immunoassayable levels of GTH I in plasma in rematuring and nonrematuring sham-operated males at this time. The control of rematuration is complex and may involve factors other than circulating GTH I levels, possibly with differences in gonadal sensitivity to GTH I.

Animals↗

PubMed1980

Alterations in nuclear structure in dark cells of the collecting tubules in the kidney of castrated rats.

Following castration, the nucleus of the dark cells of the subcortical portions of the collecting tubules in the rat kidney showed two types of morphological alterations: 1) Decomposition of the nuclear membrane in a circumscribed area. This phenomenon is independent of the sex of the animal and becomes more frequent up to the 30th day after castration. Occasionally, decomposed nuclei can also be observed in controls. 2) Giant nuclear bodies. These inclusions occurred only in castrated males. Early stages were already observed at the 10th day after orchiectomy, whereas fully developed giant nuclear bodies could not be demonstrated until about the 20th day after castration.

Animals↗

PubMed1975

Electron microscopic observations on the castration-induced X zone in the adrenal cortex of male mice.

The secondary X zone induced by castration in the adrenal cortex of adult male mice was examined by electron microscopy and radioautography with 3H-thymidine. 10-15 days after castration a thin layer of small eosinophilic cells is formed in the inner-most cortex. Such eosinophilic cells contain irregulary shaped nuclei and spherical or ellipsoidal mitochondria with tubulolamellar cristae, 20-25 days after castration a prominent zone of small eosinophilic cells was clearly identified as the secondary X zone. The typical secondary X zone cells were characterized by the formation of peculiar mitochondrial complexes and whorled sER. The X zone cells with their characteristic organelles incorporated 3H-thymidine. The ultrastructure and formation of the secondary X zone were very similar to those of the primary X zone which appears during normal postnatal development. We demonstrate here the capacity of reticularis cells of adult male mice to transform into typical X zone cells following castration.

Adrenal Cortex↗

PubMed1995

Changes in LHbeta-gene and FSHbeta-gene expression in the ram pars tuberalis according to season and castration.

Luteinizing hormone beta (LHbeta) and follicle stimulating hormone beta (FSHbeta) subunits and their mRNAs were studied in the ram pars tuberalis following different seasonal (winter vs summer) and experimental (intact vs castrated animals) conditions. Hormone-containing cells were identified by immunohistochemistry, using homologous double-stranded 35S-cDNAs. The labelling was quantified by image analysis. Immunohistochemical staining showed that cells containing LHbeta and FSHbeta were localized mainly in the ventral part of the pars tuberalis but that, in the summer, additional LHbeta containing cells were present in the dorsal part in intact rams. On the other hand, LHbeta-mRNA labelling was found in the whole pars tuberalis in wethers but only in the ventral part in intact rams. The magnitude of LHbeta-mRNA labelling was significantly greater in summer than in winter rams, and in castrated than in intact animals (P<0.001). However, the number of labelled cells was found to be the greatest in the winter (P<0.001) and was not affected by castration. FSHbeta-mRNA expression was similar to that of LHbeta-mRNA except that the level and extent were considerably lower. Thus, our results show an increase in the magnitude of gonadotropin beta subunit-mRNA in the summer and following castration; this increase appears to involve the entire pars tuberalis.

Animals↗

PubMed1982

Peptidases in the kidney and urine of rats after castration.

The localization of various peptidases in the renal section of the rat was investigated histochemically, and their activities were determined fluorometrically in renal homogenate. The membrane-bound peptidases aminopeptidase A (APA), aminopeptidase M (APM), gamma-glutamyl-transferase (gamma-GT), dipeptidylpeptidase IV (DAP IV), and the lysosomal dipeptidyl peptidases I (DAP I) and II (DAP II) were investigated in male and female (estrus) rats both before and 30 days after castration. In addition, protein excretion and APA, APM, DAP I and DAP IV activities were measured in the urine of these animals. Histochemically, the membrane-bound peptidases are demonstrable mainly in the brush borders of the proximal tubules. In addition, APA and DAP IV are found in the glomeruli, gamma-GT and DAP IV in the thin descending limbs of the loops of Henle, and gamma-GT in the basal labyrinth of the S2 and S3 segments. The lysosomal peptidases are most concentrated in the S1 and S2 segments of the proximal tubule, in the distal tubule, and in certain cells of the connecting tubule and collecting duct, where they are contained in lysosomes of varying size. Sex differences and castration effects are demonstrable both histochemically and biochemically for the investigated peptidases. Histochemically these effects are most pronounced in the S3 segments for the membrane-bound peptidases, and in the lysosomes of the proximal tubule for the lysosomal peptidases. Biochemical tests in controls show significantly higher lysosomal peptidase activities in the renal homogenate of females than of males. After castration the lysosomal peptidase activities in males increase, approaching those of females. This appears to have bearing on the sex-dependent proteinuria in rats, for lysosomal peptidases and proteinases are particularly important in the degradation of filtered proteins that are reabsorbed in the proximal tubule. In females high lysosomal peptidase activities correlate with a low proteinuria, while males demonstrate lower lysosomal peptidase activities and a significantly higher proteinuria than females. After castration, the lysosomal peptidase activities and proteinuria in males approach those in females. Renal peptidases are also excreted in the urine, again with sex differences, and so these excreted peptidases contribute to the proteinuria in rats.

Animals↗

PubMed1979

Castration for sex offenders: treatment or punishment? A review and critique of recent European literature.

The recent European literature on surgical castration in treatment of sex offenders is reviewed. Results are reported of the most important empirical studies conducted in this field of sex research in Germany, Switzerland, Norway, and Denmark. Methodological problems of follow-up studies on castrates as well as the subject of castration as treatment for sex offenders as a whole are discussed. The main conclusion is that there is no scientific or ethical basis for castration in the treatment of sex offenders.

Adult↗

PubMed1980

Depression of nocturnal pineal serotonin N-acetyltransferase activity in castrate male rats.

Pineal serotonin N-acetyltransferase (NAT) activity was examined in intact rats, castrated rats, and in rats that had been castrated and had received testosterone proprionate. Castration resulted in significantly depressing nocturnal levels of pineal NAT (p less than 0.05) when compared to enzyme activity in intact rats. Testosterone proprionate administration restored plasma LH levels to normal values in castrate rats but did not induce nocturnal pineal enzyme activity to levels seen in the pineal glands of intact rats. The data substantiate the existence of a feedback control of pineal biosynthetic activity by the hypophyseal-gonadal system, but the identity of the hormone(s) responsible for regulation of pineal NAT activity is not known.

Acetyltransferases↗

PubMed2005

Effects of castration on the expression of brain-derived neurotrophic factor (BDNF) in the vas deferens and male accessory genital glands of the rat.

Brain-derived neurotrophic factor (BDNF) is a growth factor belonging to the family of neurotrophins. Although neurotrophins in the male genital organs have been well documented, their role in the biology of these organs is far from clear. In particular, little is known about the influence of sex hormones on neurotrophin expression. In the present study, using immunohistochemistry and enzyme-linked immunosorbent assay (ELISA), we investigated the distribution and tissue concentration of BDNF in the vas deferens and accessory male genital glands in normal and castrated rats. The expression of BDNF mRNA was also investigated. In normal rats, BDNF immunoreactivity was localized in the musculature of the vas deferens and vesicular gland and in the fibromuscular stromal cells of the prostate. In the ventral prostatic lobes, BDNF immunoreactivity was localized in basal, secretory and neuroendocrine epithelial cells. Innervating ganglia and nerves were immunoreactive in all the examined tracts. After castration, BDNF immunoreactivity increased in the musculature of the vesicular gland and in the fibromuscular stromal cells of both dorsal and ventral prostatic lobes. BDNF immunoreactivity also increased in the nerves. ELISA and reverse transcription/real-time polymerase chain reaction confirmed the findings of the immunohistochemical study. In the accessory glands, castration induced an increase of both BDNF tissue concentration and mRNA expression. These results suggest that BDNF is expressed in the internal male genital organs of the rat and that its expression is downregulated by androgen hormones. We hypothesize that the observed BDNF increases are related to the castration-induced regression of the sympathetic nerves.

Androgens↗

PubMed1978

The effect of castration, thyroidectomy and haloperidol upon the turnover rates of dopamine and norepinephrine and the kinetic properties of tyrosine hydroxylase in discrete hypothalamic nuclei of the male rat.

Adult male rats were either castrated, thyroidectomized, or treated with haloperidol and the rates of turnover of dopamine (DA) and norepinephrine (NE) in the median eminence (ME), the arcuate and dorsomedial nuclei of the hypothalamus were estimated from the rate of decay of DA and NE concentrations as determined by radioenzymatic assay following blockade of catecholamine synthesis by alpha-methyl-p-tyrosine. The ME of animals similarly prepared was also examined for changes in the total activity and kinetic properties of tyrosine hydroxylase (TH). Four days following the administration of haloperidol (400 microgram/kg) or 10 days after castration, there was a significant increase in the rate of turnover of DA but not NE in the ME accompanied by an increase in the Vmax but not Km for the substrate or cofactor of TH. Furthermore, the administration of haloperidol to hypophysectomized rats also significantly increased the TH activity in the ME, indicating that such changes may occur independently of any changes in serum prolactin levels. Ten days after thyroidectomy, or three weeks after treatment with prophylthiouracil, there was a significant increase in the turnover rate of DA in both the ME and dorsomedial nucleus but not in the arcuate nucleus. No changes in the turnover rates of NE in any of the three areas were observed following thyroidectomy. In the ME, the increase in turnover of DA was accompanied by an increase in the total TH activity (Vmax) as welll as a decrease in Km for tetrahydrobiopterin but not tyrosine. From these results 4 conclusions were drawn: (1) following halperidol, castration, and thyroidectomy there are increases in the activity of dopaminergic terminals within the ME; (2) castration, haloperidol and thyroidectomy may influence the activity of dopaminergic terminals within the ME by different mechanisms; (3) changes in tyrosine hydroxylase and turnover of catecholamines within the ME may occur independently of changes in prolactin levels; and (4) local recurrent afferent circuits may exist in the arcuate nucleus region of the hypothalamus.

Animals↗

PubMed1980

Castration decreases olfactory bulb norepinephrine in male rats but not hamsters.

Norepinephrine (NE) concentrations were measured in the olfactory bulbs and olfactory cortex of male albino rats and golden hamsters sacrificed 13-16 days after castration or sham surgery. Castration significantly decreased NE concentrations in the olfactory bulbs of rats but not hamsters. Castration had no significant influence on NE levels in the olfactory cortex of either species. Regional brain weights were not influenced by surgery, and previous olfactory exposure had no significant effect on any variable measured. The decrease in rat olfactory bulb NE levels may be involved in castration-induced changes in preferences for conspecific odors.

Animals↗