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At least 685 records · Page 38Linked to original sources
PubMed1999

[Socioeconomic status and gender. Manifestations of social inequality in psychiatric diseases].

This paper is concerned with the relationship between social inequality and psychiatric disorders, an issue being neglected in German psychiatry for a long time. Social inequality will be discussed in it's dimensions "socioeconomic status" and "sex" referring to the present state of epidemiological and sociological knowledge. Because such macro-social factors offer little explanation for the association with psychiatric disorders by themselves micro-social concepts of burden and resources are necessary to link the structural and the individual level. These concepts are considered to be one pathway by which the mechanisms to disease may occur. A short review of empirical findings and analytic approaches show the complexity of these mechanisms requiring further research not only because of analytic deficiencies but also because of social change. Further research will concern in general the explanation of social patterns of diseases, in detail the link between individual stress and coping with the social structure, and last but not least a gender-sensitive approach to psychiatric disorders which is more differentiated.

Cost of Illness↗
PubMed2003

[Hermeneutic psychopathology of psychoses: scientific basis, concepts, and clinical aspects].

Besides biological psychiatry and its neurobiological field, the psychotic patient's subjectivity and his changed relations to himself and others can be isolated as a field of hermeneutic-psychopathological research. After addressing methodological problems and various approaches of hermeneutic psychopathology (transcendental-phenomenological analysis, existence analysis), the logical-analytic approach in hermeneutic psychopathology as elaborated by Müller-Suur is discussed as a way of investigating psychotic experiences and their inherent structures of meaning.Finally, the clinical relevance of hermeneutic psychopathology is examined.

Humans↗
PubMed2000

Cost-benefit analysis of prenatal diagnosis for Down syndrome using the British or the American approach.

OBJECTIVE: To compare the cost and benefits of prenatal diagnosis for Down syndrome using the British and American approaches. METHODS: This cost-benefit analysis was based on a decision-analytic approach. The British strategy included screening by a first-trimester ultrasound at 10-14 weeks for nuchal translucency thickness, and the American strategy included only second-trimester screening by using maternal age and maternal serum screening. The key probabilities of the decision-tree analysis and all cost estimates were based on American standards. The best scenario of the British strategy assumed ultrasound nuchal translucency thickness sensitivity (for detecting Down syndrome) of 80% and a false-positive rate of 5% and the worst scenario assumed a sensitivity of 50% and a false-positive rate of 10%. The results were expressed in annual costs based on approximately 4 million births per year in the United States. RESULTS: As compared with do-nothing, the American strategy was found to allow savings of approximately $96 million per year and the best scenario for the British strategy was savings of approximately $5 million per year. The financial costs of the British and American strategies would be comparable only if the first-trimester ultrasound had a sensitivity of 80% and a false-positive rate of 5% in detecting Down syndrome. CONCLUSION: The British strategy does not appear to be economically beneficial in the United States even under the most ideal scenarios of ultrasound accuracy.

Cost-Benefit Analysis↗
PubMedDate not supplied

Sequential analysis of the phasing of the medical interview.

AIMS: To study an analytical approach towards sequential analysis of the medical interview. An interview phase constitutes the analytical unit. Generally accepted phases of the medical interview are 1) medical history, 2) physical examination and 3) the conclusion segment. While descriptive and prescriptive studies claim that the sequence of the phasing is standard, it is hypothesised that in natural medical conversation the sequencing of the interview is more complex. For the doctor, the sequencing pattern is a powerful device to structure the interview and to manage the interactions within a limited time span. For the patient, especially the conclusion segment will contain more self-selection than other segments. Key concepts are turn taking, topic shift, topic flow, conversational coherence and responsiveness. METHODS: The data consisted of 800 verbatim transcript pages of 85 medical interviews obtained from general practice. Interplay of theoretical notions and data-driven observations produced a reliable analytical method described in the article. The method enables to study processes of (a)symmetry in medical communication and the ways to deal with problems of misunderstanding. RESULTS: Results confirmed 1) the asymmetrical character of the interview, 2) the complexity of the phasing, and 3) the existence of several types of interviews. CONCLUSIONS: The method can be applied for a broad range of research questions.

Communication↗
PubMed2006

Motion as a phenotype: the use of live-cell imaging and machine visual screening to characterize transcription-dependent chromosome dynamics.

BACKGROUND: Gene transcriptional activity is well correlated with intra-nuclear position, especially relative to the nuclear periphery, which is a region classically associated with gene silencing. Recently however, actively transcribed genes have also been found localized to the nuclear periphery in the yeast Saccharomyces cerevisiae. When genes are activated, they become associated with the nuclear pore complex (NPC) at the nuclear envelope. Furthermore, chromosomes are not static structures, but exhibit constrained diffusion in real-time, live-cell studies of particular loci. The relationship of chromosome motion with transcriptional activation and active-gene recruitment to the nuclear periphery has not yet been investigated. RESULTS: We have generated a yeast strain that enables us to observe the motion of the galactose-inducible GAL gene locus relative to the nuclear periphery in real-time under transcriptionally active and repressed conditions. Using segmented geometric particle tracking, we show that the repressed GAL locus undergoes constrained diffusive movement, and that transcriptional induction with galactose is associated with an enrichment in cells with GAL loci that are both associated with the nuclear periphery and much more constrained in their movement. Furthermore, we report that the mRNA export factor Sac3 is involved in this galactose-induced enrichment of GAL loci at the nuclear periphery. In parallel, using a novel machine visual screening technique, we find that the motion of constrained GAL loci correlates with the motion of the cognate nuclei in galactose-induced cells. CONCLUSION: Transcriptional activation of the GAL genes is associated with their tethering and motion constraint at the nuclear periphery. We describe a model of gene recruitment to the nuclear periphery involving gene diffusion and the mRNA export factor Sac3 that can be used as a framework for further experimentation. In addition, we applied to the analysis of chromosome motion a machine visual screening approach that used unbiased visual data rather than segmented geometric data. This novel analytical approach will allow for high-throughput study of processes that can be monitored via alterations in chromosome motion and connectivity with the nuclear periphery.

Cell Survival↗
PubMedDate not supplied

[First SIVR list of reference values: definitions, methodologic criteria and analytical strategies].

In this paper, a list of reference values for trace elements and some of the main organic xenobiotics and/or their metabolites in biological matrices (urine, serum or blood) is presented. This list has been made out taking into account several variables, such as sex, diet, alcohol and drugs consumption, smoking habits, living area, etc. and it is referred to as the 1st SIVR list 2001. In addition, the SIVR research activity is reassessed on the basis of the availability of multielement techniques and new analytical approaches. In particular, to define the reference values for PCB, selection criteria and analytical methodologies are discussed with a view to choosing the more significant congeners and the most suitable technique. In the end, the results from two SIVR projects concerning the determination of haematic Pb and urinary Hg are synthesized.

Chemistry, Clinical↗
PubMed2000

Towards European urinalysis guidelines. Introduction of a project under European Confederation of Laboratory Medicine.

Improved standardized performance is needed because urinalysis continues to be one of the most frequently requested laboratory tests. Since 1997, the European Confederation of Laboratory Medicine (ECLM) has been supporting an interdisciplinary project aiming to produce European urinalysis guidelines. More than seventy clinical chemists, microbiologists and ward-based clinicians, as well as representatives of manufacturers are taking part. These guidelines aim to improve the quality and consistency of chemical urinalysis, particle counting and bacterial culture by suggesting optimal investigative processes that could be applied in Europe. The approach is based on medical needs for urinalysis. The importance of the pre-analytical stage for total quality is stressed by detailed illustrative advice for specimen collection. Attention is also given to emerging automated technology. For cost containment reasons, both optimum (ideal) procedures and minimum analytical approaches are suggested. Since urinalysis mostly lacks genuine reference methods (primary reference measurement procedures; Level 4), a novel classification of the methods is proposed: comparison measurement procedures (Level 3), quantitative routine procedures (Level 2), and ordinal scale examinations (Level 1). Stepwise strategies are suggested to save costs, applying different rules for general and specific patient populations. New analytical quality specifications have been created. After a consultation period, the final written text will be published in full as a separate document.

Europe↗
PubMed2004

Characterization of cigarette tobacco by direct electrospray ionization-ion trap mass spectrometry (ESI-ITMS) analysis of the aqueous extract--a novel and simple approach.

In support of the efforts to combat smuggling, as well as illegal sale and distribution of cigarettes, an analytical approach for the characterization of tobacco has been proposed and evaluated. It involves aqueous extraction of the filler tobaccos followed by direct analysis of the extracts by electrospray ionization-ion trap mass spectrometry (ESI-ITMS) in the negative mode. Typically, the deprotonated ions, [M - H](-), of organic acids (malic, citric, caffeic, quinic acid) and polyphenols (chlorogenic acid, rutin, scopoletin) were detected. MS/MS spectra of the ion at m/z 191, which is the [M - H](-) of quinic acid, citric acid, and scopoletin, and a fragment ion of chlorogenic acid were acquired. Significant differences in the MS and MS/MS spectra were observed between counterfeit samples and the corresponding authentic brand name cigarettes. Analysis of 25 commercial cigarettes showed that straight Virginia blends were readily distinguished from the blended products containing different tobacco types (Virginia, burley, and Oriental). The former exhibited consistently higher relative abundances of m/z 353 (chlorogenic acid) to m/z 133 (malic acid) in the MS spectra (0.9-1.2 vs 0.4-0.6) and higher intensity ratios of m/z 176 (scopoletin) to m/z 173 (0.4-0.8 vs 0.1-0.3) and of m/z 127 (quinic acid) to m/z 173 (0.7-1.0 vs 0.3-0.5) in the MS/MS spectra. Evidence is presented to demonstrate that the spectral differences were related not only to the tobacco type (Virginia, burley and Oriental) but also to the tobacco part (stem, lamina) used in the manufacture of the cigarettes.

Caffeic Acids↗
PubMed2004

Bayesian inference of the metazoan phylogeny; a combined molecular and morphological approach.

Metazoan phylogeny remains one of evolutionary biology's major unsolved problems. Molecular and morphological data, as well as different analytical approaches, have produced highly conflicting results due to homoplasy resulting from more than 570 million years of evolution. To date, parsimony has been the only feasible combined approach but is highly sensitive to long-branch attraction. Recent development of stochastic models for discrete morphological characters and computationally efficient methods for Bayesian inference has enabled combined molecular and morphological data analysis with rigorous statistical approaches less prone to such inconsistencies. We present the first statistically founded analysis of a metazoan data set based on a combination of morphological and molecular data and compare the results with a traditional parsimony analysis. Interestingly, the Bayesian analyses demonstrate a high degree of congruence between morphological and molecular data, and both data sets contribute to the result of the combined analysis. Additionally, they resolve several irregularities obtained in previous studies and show high credibility values for controversial groups such as the ecdysozoans and lophotrochozoans. Parsimony, on the contrary, shows conflicting results, with morphology being congruent to the Bayesian results and the molecular data set producing peculiarities that are largely reflected in the combined analysis.

Animals↗
PubMed2005

Rapid, simultaneous determination of headspace oxygen and moisture in pharmaceutical packages using muGC.

The rapid, accurate determination of headspace oxygen and moisture in various pharmaceutical packages is important for both product packaging development and the implementation of new packaging technologies. Current headspace oxygen measurement techniques suffer from serious drawbacks in terms of potential sampling contamination, lengthy analysis times, and large required analysis volumes. In addition, relatively few techniques currently exist for the convenient determination of headspace moisture in packaging systems. Efforts herein focused on the development and application of a new method for the rapid and simultaneous determination of headspace oxygen and moisture in pharmaceutical packages using micro-gas chromatography (muGC). Studies showed that both headspace oxygen and moisture could be simultaneously quantified in <90 s on sample volumes of 50-100 microL by employing muGC with dual chromatographic analysis modules. Sampling issues common to manual syringe-based injections were also alleviated in the current studies by use of the built-in diaphragm pump sampling interface of the portable muGC system. The performance of the analytical approach was evaluated and shown to exhibit excellent linearity, accuracy, and precision for both analytes. High sensitivity for headspace oxygen was demonstrated, allowing for levels of oxygen as low as 0.03% to be accurately quantified. The subject method was applied to measure the headspace oxygen and moisture in pharmaceutical blister packaging and glass vials.

Chromatography, Gas↗
PubMedDate not supplied

An actuarial model approach to assessing the impact of serial testing for prostate cancer using the Tandem PSA assay.

This article summarizes an analytical approach to modeling prostate cancer detection scenarios that health-care plans, i.e., managed care organizations, can use to assist them in making early detection policy decisions. The actuarial modeling approach incorporates current variations in clinical practice with costs for the purpose of generating per member per month outputs. The intent of this approach is to provide laboratories with a tool to provide a value-added service to their managed care customers. The model can be tailored to an array of managed care plans and payer financial arrangements. Included here is a summary of an oral presentation from the Outcomes Conference at the CLMA meeting in Minneapolis, Minnesota in August of 1995. This material was presented by Robert Parson.

Actuarial Analysis↗
PubMed1985

Capsule chemistry technology for high-speed clinical chemistry analyses.

We describe a new analytical approach--"capsule chemistry"--for high-speed, selective analysis of a wide variety of analytes. Sequential micro-aliquots of sample and reagents are encapsulated within an inert fluorocarbon liquid. The resulting "test capsule" is introduced into a single analytical flow path, composed of a solid fluorocarbon, Teflon, where the sample is incubated, mixed, reacted, and measured as a moving series of individual tests. These randomly selective assays are processed at a rate of 720 per hour. The unique physical interaction between the liquid and solid fluorocarbon carrier materials effectively prevents detectable "carryover" of aqueous constituents between the successive test capsules. Reactions are monitored through the walls of the Teflon analytical channel at nine in-line detector stations for colorimetric and nephelometric measurements.

Autoanalysis↗
PubMed2004

Linear approach to axial resolution in elasticity imaging.

Thus far axial resolution in elasticity imaging has been addressed only empirically. No clear analytical approaches have emerged because the estimator is non-linear in the data, correlation functions are nonstationary, and system responses vary spatially. This paper describes a linear systems approach based on a small-strain impulse approximation that results in the derivation of a local impulse response (LIR) and local modulation transfer function (LMTF). Closed-form solutions for strain LIR are available to provide new insights on the role of instrumentation and processing on axial strain resolution. Novel phantom measurements are generated to validate results. We found that the correlation window determines axial resolution in most practical situations, but that the the same system properties that determine B-mode resolution ultimately limit elasticity imaging.

Animals↗
PubMed2003

"Mind the gaps": an empirical approach to engineering ethics, 1997-2001.

A survey on ethical issues in engineering was administered over a five-year period to Stanford engineering students and practicing engineers. Analysis of its results strongly suggests that important disconnects exist between the education of engineering students regarding ethical issues in engineering on the one hand, and the realities of contemporary engineering practice on the other. Two noteworthy consequences of these gaps are that the views of engineering students differ substantially over what makes an issue an ethical issue, while practicing engineers exhibit significant disagreement over what is the most important non-technical aspect of being a responsible engineering professional in contemporary society. These divergences impede the recognition of ethical issues and of specific moral responsibilities of engineers in concrete professional practice. It is argued that the use of suitably refined and probing surveys of engineering students and practicing engineers about ethical issues in engineering is an important although neglected empirical approach to the study of engineering ethics. Such an approach can enhance the prevailing case study method and combat over-tidy theoretical-analytical approaches to the subject.

Attitude↗
PubMed1999

Functional analysis and response covariation in the assessment of personality disorders: a reply to Staats and to Bissett and Hayes.

In this reply to Bissett and Hayes (this issue) and Staats (this issue) we address critical comments in response to our initial proposal and highlight points of agreement. The overall thesis of our reply is that classification schemes based on nomothetic response covariation, such as DSM, complement, but do not substitute for, an idiographically-based functional analysis and behavioral assessment. In the context of our reply, we address the following primary concerns raised by Bissett, Hayes, and Staats: (a) we are essentially proposing the melding of two theoretically incongruent approaches, and that such a melding is inherently not viable or useful; (b) the behavior analytic approach cannot account for personality or psychological constructs; and (c) that categories based on topography do not have demonstrated treatment utility. We also discuss points of agreement with our respondents: (d) a theoretically-based descriptive classification system is required to ultimately advance clinical science, (e) the DSM personality disorder classification system, to remain viable, needs a stronger empirical base; and (f) that alternatives to DSM classification that more strongly emphasize behavioral principles are in need of development.

Behaviorism↗
PubMed1988

Effects of parental smoking and other risk factors on the development of pulmonary function in children and adolescents. Analysis of two longitudinal population studies.

Two sets of longitudinal pulmonary function data have been analyzed to determine the effects of parental smoking on the development of lung function in children and adolescents. One community population was obtained in East Boston, Massachusetts, and one in Tucson, Arizona; both studies started in the 1970s and are still continuing. These data sets, analyzed by different methods, have yielded different answers in regard to the effects of parental smoking. A common analytical approach is now used to determine whether the different outcomes were due to the different analytical methods previously used. These results were compared with results from a similar parallel analysis conducted by the East Boston investigators and indicate that the differences found were not due to the methods used for analysis. Reasons for the differences found are explored. The most likely factor responsible for the disparate results is the exposure difference in the two populations, related to proportion and amount of maternal smoking and different indoor environments.

Adolescent↗
PubMed1998

Zero effects in substance abuse programs. Avoiding false positives and false negatives in the evaluation of community-based programs.

The evaluation of community-based programs poses special design and analysis problems. The present article focuses on two major types of errors that can occur in such evaluations: false positive--incorrectly declaring a program to be effective--and false negatives--incorrectly declaring a program to be ineffective. The evaluation of a national demonstration of community-based programs to reduce substance abuse, Fighting Back, is used to illustrate several approaches to reduce the probability of errors. Both those errors that are affected by the design and those by analytic approaches are considered. Ways to assess multiple outcomes and to match the complexity of the program with design and analytic strategies are proposed. Community trials are complex interventions, and, although they can provide very useful information, their outcomes have to be understood in terms of the constructs they test and the contexts within which they are carried out.

Adolescent↗
PubMed2005

Determination of fexofenadine in tablets by capillary electrophoresis in free solution and in solution with cyclodextrins as analyte carriers.

Capillary electrophoresis (CE) methods for the determination of fexofenadine (FEX) in commercial pharmaceuticals were developed. It was demonstrated that FEX could be effectively analyzed in free solution cationic CE at low pH. Another analytical approach studied was based on cyclodextrin (CD) modified CE where highly charged CD derivatives served as analyte carriers. In this way, the separation range was spread to physiological pH region and a CE analysis of FEX, present actually in its zwitterionic form, could be accomplished. Several parameters affecting the separations were studied, including the type and concentration of carrier ion, counterion, analyte carrier, and pH of the buffer. The methods based on the free solution CE and CD-modified CE were compared each other, validated, and applied for the determination of FEX in tablets.

Anti-Allergic Agents↗