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The cellular lining of the inner wall of Schlemm's canal was anatomically different at different pressure levels. When the anterior chamber pressure rose above that of the canal, there was a marked increase in the number and size of the vacuoles present. At the same time the canal became smaller. When Schlemm's canal pressure exceeded that of the anterior chamber, the vacuoles decreased in size and number, and the canal enlarged. These findings were independent of postmortem artifacts.
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