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Disseminated visceral coccidiosis and cloacal cryptosporidiosis in a Japanese white-naped crane (Grus vipio).

A 4-mo-old male Japanese white-naped crane (Grus vipio) kept in an outdoor exhibit at the Everland Zoological Gardens in Korea became depressed and developed anorexia, weight loss, and diarrhea. Death of this bird was associated with an overwhelming systemic infection by an intracellular coccidian parasite, which resulted in necrosis and granulomatous inflammation in a number of major organs, including the intestine, liver, spleen, and kidney. Coccidian parasite-laden macrophages were commonly found in the blood vessels of these organs. Using electron microscopy and polymerase chain reaction assays, the parasite was identified as Eimeria sp. The bird was also infected with Cryptosporidium sp., which suggests an immunosuppressed state, although the cause of such suppression was not identified. Our findings suggest that an initial Eimeria sp. intestinal infection spread to other organs through the blood vessels, with the immunosuppressed state possibly contributing to a rapid hematogenous transmission. To our knowledge, this is the first report of disseminated visceral coccidiosis caused by Eimeria sp. in a captive Japanese white-naped crane.

Animals↗

The neuro-endocrine complex in the gastro-entero-pancreatic endocrine system.

The pancreatic islet is one of the well-studied endocrine tissues in regard to innervation. This neuro-insular complex has been known since the 1920's. The direct contact of the nervous element to the gastro-enteric endocrine cells has recently been reported in the proventricular mucosa of the finch. Here, the majority of the endocrine cells lie in the lamina propria and form a neuro-endocrine complex with unmyelinated nerve fibers. A similar neuro-endocrine complex was also reported in the rat stomach and in the duodenum of the human fetus. The occurrence of the neuro-endocrine complexes in the gastro-entero-pancreatic endocrine system might provide supportive data to the neuro-ectodermal theory on the origin of the endocrine cells proposed by Professors A. G. E. PEARSE and T. FUJITA.

Animals↗

Status of the digestive system and tibiae of cropectomized hens at night.

Experiments were conducted to determine the effect of cropectomy on the total amount and calcium content of ingesta in the digestive tract of the laying hen at night, as well as to determine if any abnormal physiological changes had occurred in the cropectomized hens. Treatments were cropectomized, sham operated and unoperated control hens. The gizzards from cropectomized hens contained about twice as much feed at 7:30 p.m. as that of the controls. The gizzard weight, without contents, was also significantly heavier in cropectomized hens than that of the controls. At 7:30 p.m. total contents of the digestive system of cropectomized and control hens were 15.94 gm. versus 24.07 gm., respectively. The control birds also had 2.71 times more total calcium in the digestive system at 7:30 p.m. than the cropectomized birds. The contents of the small intestine of cropectomized birds had 17% more calcium at 7:30 p.m. than did that of the controls. However, by 5:30 a.m. the contents of the small intestine of the control birds had 3.17 times more calcium than did that of the cropectomized birds. No differences were found between treatments or between time periods in relation to tibia breaking strength, calcium content of tibia or percent tibia ash. The degree of dietary calcium deficiency at night appears to be greater with cropectomized hens, even though the gizzard compensates, in part, for the loss in storage capacity of the crop. It is hypothesized from these data that the crop is an important and necessary organ in the laying hen for supplying nutrients throughout the night when the hen is not consuming feed.

Animal Feed↗

Comparative toxicity of Chaetomium contaminated corn and various chemical forms of oosporein in broiler chicks.

The toxicity to broiler chicks of Chaetomium contaminated corn and various chemical forms of oosporein were compared by feeding diets containing 60% Chaetomium contaminated corn (300 micrograms oosporein/g diet), and 300 or 150 micrograms/g of purified oosporein in either the K salt, Na salt, or organic acid form from hatching to 3 weeks of age. The Chaetomium contaminated corn diet caused 100% mortality during the first week of feeding. Necropsies revealed extensive visceral and articular gout, enlarged pale kidneys, dehydration, proventricular enlargement with mucosal necrosis, and a dark green discoloration of the gizzard lining. When the mortality percentages of the two experiments conducted were considered collectively, the K and Na salts of oosporein caused significantly higher mortality than the organic acid form of oosporein. The K salt caused the most severe lesions and the organic acid caused the least severe lesions. No mortality occurred at the 150 micrograms/g K salt or 150 micrograms/g organic acid levels. Relative kidney weights were increased by all forms of oosporein at 300 micrograms/g, but at 150 micrograms/g only the K salt caused an increase in kidney weight. The LD50 values, based on mortality from 1 to 10 days, were 5.77, 5.00, and 4.56 mg/kg for oosporein acid, oosporein Na salt, and oosporein K salt, respectively. These results suggest that the salts of oosporein (particularly the K salt) are more toxic than the organic acid, and the natural occurrence of oosporein in a salt form could contribute to the increased toxicity of the Chaetomium contaminated corn.

Animals↗

Scirpentriol toxicity in young broiler chickens.

Scirpentriol (STO) (3 alpha,4 beta,15-trihydroxy-12,13-epoxytrichothec-9- ene), the parent alcohol of the family of acetylated scirpenol mycotoxins produced by several Fusarium species, has been implicated in mixed toxicoses of animals, but there is not a general description of its toxicity in chickens. Dietary STO (0, 2, 4, 8, 16, and 32 micrograms/g feed) was fed to four groups of 10 male day-old broiler chickens for 3 wk. The minimum effective dose (MED) for reducing growth rate significantly (P less than .05) was 4 micrograms/g. The same MED was found for increased serum alkaline phosphatase and relative weight of the gizzard. Unlike literature reports for two other trichothecene mycotoxins, T-2 toxin and diacetoxyscirpenol (DAS), STO impaired feed conversion efficiency but did not alter spleen or pancreas size. The MED of STO for decreases in serum lactic dehydrogenase and aspartate aminotransferase was 8 micrograms/g, but the MED for decreased serum albumin and total proteins and regression of the bursa of Fabricius was 16 micrograms/g. Serum sodium, potassium, and calcium were not altered at the highest dose, 32 micrograms/g, but serum phosphate, uric acid, and cholesterol were decreased by 32 micrograms/g. Serum chloride was increased slightly but significantly (P less than .05) at 16 and 32 micrograms/g. Based on these results, STO toxicosis of chickens can be differentiated from those of T-2 toxin and DAS and its toxicity appears sufficient to warrant further attention.

Alkaline Phosphatase↗

Effects of feeding Fusarium moniliforme culture material, containing known levels of fumonisin B1, in the young turkey poult.

The effects of feeding Fusarium moniliforme culture material, containing known concentrations of fumonisin B1 (FB1), were studied in turkey poults. Day-old poults were allotted randomly to dietary treatments containing 0, 0.41, 0.82, 1.23, 1.64. 2.87, 4.10, 5.33, 6.56, and 7.79% fumonisin culture material (FCM). These levels of FCM supplied 0, 25, 50, 75, 100, 175, 250, 325, 400, and 475 mg FB1/kg of feed. Each dietary treatment was fed to six pen replicates of six poults each for 21 d. Poults fed FCM that supplied 325 to 475 mg FB1/kg diet had lower (P < 0.05) feed intakes and BW gains. Increased (P < 0.05) liver and pancreas weights were observed in poults fed FCM that supplied > or = to 175 mg FB1/kg. Poults fed FCM that supplied 400 and 475 mg FB1/kg diet had increased (P < 0.05) red blood cell counts and increased (P < 0.05) serum concentrations of gamma glutamyl transferase and aspartate aminotransferase. Compared with controls, poults fed FCM that supplied 25, and 75 to 475 mg FB1/kg had increased (P < 0.05) liver sphinganine:sphingosine ratios. Hepatocellular hyperplasia was mild at 75 and 100 mg FB1/kg diet, moderate to severe at 250 mg/kg FB1, and severe at 325 to 475 mg FB1/kg. Multifocal to generalized loss of cross striations and thinning of cardiomyocytes was observed in poults fed FCM that supplied 475 mg FB1/kg diet. Results indicated that diets containing < or = to 1.23% FCM that supplied > or = to 75 mg FB1 /kg are toxic to young turkeys.

Animals↗

Ecology of helminth parasitism in bobwhites from northern Florida.

Examination of 700 northern bobwhites (Colinus virginianus), 50 each February from 1971 through 1984, from Tall Timbers Research Station, Leon County, Florida, disclosed 15 species of helminth parasites. Nine species (Raillietina cesticillus, R. colinia, Aproctella stoddardi, Cheilospirura spinosa, Cyrnea colini, Dispharynx nasuta, Heterakis isolonche, Tetrameres pattersoni, and Trichostrongylus tenuis) generally were found on an annual basis and were considered characteristic components of the helminth fauna. Infrequently found species were Brachylecithum nanum, Rhabdometra odiosa, Capillaria sp., Gongylonema ingluvicola, H. gallinarum, and Oxyspirura matogrosensis. Intensities of C. colini and H. isolonche differed among host sex and age classes, and prevalences and/or intensities of A. stoddardi, C. spinosa, T. pattersoni, and T. tenuis differed between host age classes. Prevalences and/or abundances of seven species (R. cesticillus, R. colinia, C. spinosa, C. colini, H. isolonche, T. pattersoni, and T. tenuis) varied with bobwhite density, apparently because bobwhites were either the primary or only definitive host on the area. Two species (A. stoddardi and D. nasuta) did not vary with bobwhite density, apparently due to the buffering effect of a broad range of definitive hosts on the area. Prevalences and/or intensities of R. colinia, C. spinosa, and T. tenuis differed with agricultural fields status (cultivated versus fallow) suggesting that land use and its attendant habitat changes influenced transmission of these species. The occurrence of C. spinosa and T. pattersoni in individual bobwhites was not independent and was attributed to utilization of the same species of grasshoppers as intermediate hosts. Localized tissue damage and inflammation were associated with A. stoddardi, D. nasuta, C. spinosa, C. colini and T. pattersoni. Decreases in body weight in juvenile bobwhites were associated with increasing intensities of H. isolonche and T. tenuis. The observed relationships to bobwhite density and other variables are discussed with regard to known aspects of life histories of the nine most common species.

Age Factors↗

Serologic and parasitologic survey of the endangered Attwater's prairie chicken.

Because conservation biologists have postulated that infectious diseases may have potentiated the endangerment of the Attwater's prairie chicken (Tympanuchus cupido attwateri), free-living prairie chickens were surveyed from all remaining populations for helminthic endoparasites and antibody against the etiological agents of nine infectious diseases. Samples from 4 of 27 adult males were positive for anti-Pasteurella multocida antibody. All other serologic tests were negative (n = 19). We identified Dispharynx nasuta, a parasite previously associated with disease in other grouse from North America, in one of three adult Attwater's prairie chickens examined. Evidence of Trichostrongylus cramae was found for eight of nine suitable samples, which represents the first report of this parasite in prairie grouse. The mean intensity of T. cramae in Attwater's prairie chicken was 1,019.3 (Range = 3-1,906; n = 3). Further work is needed to determine whether P. multocida, T. cramae, or D. nasuta are detrimental to Attwater's prairie chicken populations. If so, conservation biologists could reduce the prevalence and incidence of these parasites and potentially gain more time to address the habitat conditions thought to be the ultimate cause of population declines.

Animals↗