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Application of conducting polymers to biosensors.

Recently, conducting polymers have attracted much interest in the development of biosensors. The electrically conducting polymers are known to possess numerous features, which allow them to act as excellent materials for immobilization of biomolecules and rapid electron transfer for the fabrication of efficient biosensors. In the present review an attempt has been made to describe the salient features of conducting polymers and their wide applications in health care, food industries, environmental monitoring etc.

Biosensing Techniques↗

Insecticides for tsetse and trypanosomiasis control: is the environmental risk acceptable?

Tsetse control technology, as well as the science of environmental impact and risk assessment, has come a long way over the past 20 years. The application of science to tsetse control has significantly reduced impacts on the environment, and the costs of environmental monitoring and assessment have reduced dramatically. Environmental impacts are encountered with all insecticide-based techniques, but they appear to be relatively minor and short-lived. Despite a wealth of ecotoxicological information, conflict between real and imagined risks is hampering decision making. As discussed here by Ian Grant, public confidence in control technologies is being undermined by political viewpoints, fears (often based on misinformation) and poor communication of the environmental issues.

Animals↗

A new mussel certified reference material (CRM 477) for the quality control of butyltin determination in the marine environment.

The analytical techniques used for the determination of butyltin compounds in biological tissues are generally based on a succession of steps (e.g., extraction, derivatization, separation, detection) which are all prone to systematic errors. An interlaboratory programme performed at the EU level and based on a stepwise approach has enabled one to identify and remove most of these errors and to improve considerably the state of the art so that certification of a mussel reference material has been made possible. This paper describes the preparation of this mussel reference material containing monobutyltin (MBT), dibutyltin (DBT) and tributyltin (TBT), the homogeneity and stability studies and the analytical work performed for the certification of the contents of MBT [Sn(C4H9)3+], DBT [Sn(C4H9)2(2+)] and TBT [Sn(C4H9)3+]. The results obtained by a group of expert EU laboratories are discussed along with the methods used to certify the mass fractions (based on dry mass) of monobutyltin (1.50 +/- 0.28 mg kg-1 as MBT cations), dibutyltin (1.54 +/- 0.12 mg kg-1 as DBT cations) and tributyltin (2.20 +/- 0.19 mg kg-1 as TBT cations). The paper also describes an attempt to certify the content of triphenyltin in this material which, although reasonable agreement was found among the participants' results, failed owing to the instability of this compound. This new material will be of great support to ensure the quality control of butyltin determination in shellfish, as carried out in environmental monitoring programmes.

Animals↗

Radiation safety training program at the National Institutes of Health.

At the U.S. Public Health Service's National Institutes of Health (NIH), one of the world's largest biomedical and clinical research institutions, 70 to 80% of all research is performed using radiation or radioactive materials. To maintain a safe environment and minimize risks to the health and safety of the employees, patients, visitors, and the surrounding community, a radiation protection program is required. Under U.S. Nuclear Regulatory Commission licenses and NIH policies, the Radiation Safety Branch (RSB), Division of Safety, administers a comprehensive radiation safety program covering over 3,500 NIH laboratories and some 7,000 laboratory and ancillary staff workers. The NIH radiation protection program provides for the effective supervision, control, and monitoring of all radioactive materials and radiation sources at NIH. This includes activities such as radiation exposure monitoring, laboratory inspections, waste management, consulting, environmental monitoring, and training. Training is considered an integral part of the radiation safety program. RSB offers an extensive training program with over 20 different training courses each year. For example, in 1993 some 6,700 employees attended 243 classes.

Humans↗

Remotely sensed data for ecosystem analyses: combining hierarchy theory and scene models.

Remotely sensed data have been used extensively for environmental monitoring and modeling at a number of spatial scales; however, a limited range of satellite imaging systems often constrained the scales of these analyses. A wider variety of data sets is now available, allowing image data to be selected to match the scale of environmental structure(s) or process(es) being examined. A framework is presented for use by environmental scientists and managers, enabling their spatial data collection needs to be linked to a suitable form of remotely sensed data. A six-step approach is used, combining image spatial analysis and scaling tools, within the context of hierarchy theory. The main steps involved are: (1) identification of information requirements for the monitoring or management problem; (2) development of ideal image dimensions (scene model), (3) exploratory analysis of existing remotely sensed data using scaling techniques, (4) selection and evaluation of suitable remotely sensed data based on the scene model, (5) selection of suitable spatial analytic techniques to meet information requirements, and (6) cost-benefit analysis. Results from a case study show that the framework provided an objective mechanism to identify relevant aspects of the monitoring problem and environmental characteristics for selecting remotely sensed data and analysis techniques.

Conservation of Natural Resources↗

Methodological scheme for designing the monitoring of genetically modified crops at the regional scale.

According to EC regulations the deliberate release of genetically modified (GM) crops into the agro-environment needs to be accompanied by environmental monitoring to detect potential adverse effects, e.g. unacceptable levels of gene flow from GM to non-GM crops, or adverse effects on single species or species groups thus reducing biodiversity. There is, however, considerable scientific and public debate on how GM crops should be monitored with sufficient accuracy, discussing questions of potential adverse effects, agro-environmental variables or indicators to be monitored and respective detection methods; Another basic component, the appropriate number and location of monitoring sites, is hardly considered. Currently, no consistent GM crop monitoring approach combines these components systematically. This study focuses on and integrates spatial agro-environmental aspects at a landscape level in order to design monitoring networks. Based on examples of environmental variables associated with the cropping of Bt-Maize (Zea maize L.), herbicide-tolerant (HT) winter oilseed rape (Brassica napus L.), HT sugar beet (Beta vulgaris L.), and starch-modified potato (Solanum tuberosum L.), we develop a transferable framework and assessment scheme that comprises anticipated adverse environmental effects, variables to be measured and monitoring methods. These we integrate with a rule-based GIS (geographic information system) analysis, applying widely available spatial area and point information from existing environmental networks. This is used to develop scenarios with optimised regional GM crop monitoring networks.

Beta vulgaris↗

Statistical model for assessing the quality and cost of inspection procedures in forensic and environmental analysis.

Legal regulations on the composition and safety of food products require inspection programmes to maintain the standards required or to obtain an insight in the real situation. Such programmes consist of a sampling procedure followed by analysis of the samples. When monitoring environmental contamination similar programmes are required. When large numbers of samples have to be analysed in order to monitor contaminants in either environmental or food-chain samples, and the majority fulfil the legal requirements, a two-stage control system is desirable. Firstly, samples are analysed by a simple 'screening method', to sift out the large number of samples that fulfil the requirements (negative results). The minority not fulfilling the requirements (positive results) are investigated further by a more sophisticated confirmatory method, specific for the analyte(s) of interest, for an ultimate judgement. For this type of inspection a simple model is used in this study, in order to calculate the fraction of false negative results in the final decision. It is assumed, that, when the quality criteria for the confirmatory method are appropriate, the fraction of false positive results is negligibly small. The quality of the inspection is expressed in terms of the fraction f of false negative results with respect to the real positive samples and is related to labour and costs. In an example given, the costs of analysing 40,000 samples with f = 6.6% are ecu 7,800,000. Improving f down to 3.2% creates additional costs of ecu 16,000,000. The proposed model might not be sufficiently realistic, but more realistic models can be implemented.(ABSTRACT TRUNCATED AT 250 WORDS)

Costs and Cost Analysis↗

The ecological condition of Gulf of Mexico resources from Perdido Key to Port St. Joe, Florida, USA: part I. coastal beach resources.

Using the approach established by EPA's Environmental Monitoring and Assessment Program (EMAP), a shoreline monitoring survey was conducted in August and September 1999, encompassing the Florida Panhandle from Perdido Key, Florida to Port St. Joe, Florida. The objective of this survey was to demonstrate the use of a probabilistic survey for monitoring and estimating the condition of swimmable beach areas. Thirty stations were sampled using a probabilistic sampling design. Hydrographic data were collected in addition to samples for water chemistry. Bacterial indicators, enterococci and fecal coliforms, were enumerated from the water according to the EPA Beaches Environmental Assessment Closure and Health (BEACH) Program and Florida state guidelines. Additional criteria for site condition included the presence or absence of primary and secondary dunes, anthropogenic debris and vegetation. Based on EMAP evaluation guidelines and Florida state criteria, a baseline assessment of the condition of the Gulf of Mexico beach resources surveyed is presented.

Data Collection↗

Sulphadimethoxine and Azolla filiculoides Lam.: a model for drug remediation.

Plants can be an interesting tool for in situ remediation of drug contaminated waters. In a laboratory model Azolla filiculoides Lam., an aquatic fern known to absorb pollutants, has been exposed to an environmental persistent antibiotic commonly used in intensive farming, sulphadimethoxine (S), to test its bioremediation capability. In a 5 week experiment, plants were cultivated outdoor at four drug concentrations (50, 150, 300 and 450 mg l(-1)) in N-free mineral medium. Drug affects growth rate (as biomass yield per week), N2-fixation, heterocyst frequency, but plants are able to survive. Notwithstanding, at all concentrations tested drug was actively removed from the medium and the accumulation in the biomass is in order of magnitude up to mg g(-1) plant dry weight (1000 ppm). Drug uptake and degradation rates increase with S concentrations in the culture medium. The efficacy of the model was very high. These results demonstrated that Azolla can be taken into consideration as a tool for sulphonamides environmental monitoring and decontamination.

Adsorption↗

Windchill index and military applications.

A new Windchill Apparent Temperature (WCT) has been introduced to replace the Windchill Index (WCI) and Windchill Equivalent Temperature (WCET) used to quantify cold exposure. From the time of its introduction the WCI has been criticized on scientific grounds. Despite a history of criticism, the WCI and the derived WCET have been adopted by military and civilian organizations to characterize the hazards presented by exposure to cold environments. However, the military has specific needs that differ from those of the civilian population. Thus, additional weather products and devices, including thermoregulatory models, environmental monitors, and personal physiological status monitors, are available to supplement the revised WCT.

Bias↗

Proteomics in environmental pollution research: Advances, challenges, and future directions.

Environmental proteomics has emerged as a powerful approach for elucidating the molecular mechanisms underlying pollutant-induced biological effects. Although this field has developed rapidly, the systematic review of recent proteomics applications in environmental pollution research remains limited. This review explored the emerging roles of toxicoproteomics in biomarker discovery and mechanistic elucidation, as well as ecotoxicoproteomics in ecological risk assessment and bioremediation strategies. Here, we review the field, highlighting recent trends such as the integration of proteomics with genomics, transcriptomics, and metabolomics to provide a comprehensive view of biological responses to environmental stressors. We further discuss the growing application of artificial intelligence in improving proteomics data interpretation and accelerating biomarker discovery. In addition, recent technological advances in environmental proteomics are highlighted, including next-generation tissue microarray proteomics, nanoscale proteomics, single-cell proteomics, and spatial proteomics. Despite its potential, proteomics faces challenges, such as high operational costs, computational complexity in analysis, and technical limitations in low-abundance protein detection. We propose that the convergence of proteomics with artificial intelligence and multi-omics approaches offers promising solutions to these challenges, enhancing the practical application of proteomics in environmental monitoring and risk assessment.

Proteomics↗

Urinary platinum in hospital personnel occupationally exposed to platinum-containing antineoplastic drugs.

Urinary platinum levels of 21 nurses and hospital pharmacy personnel occupationally exposed to platinum containing antineoplastic drugs were determined in 24-h urine by voltammetric analysis after UV photolysis. All study participants applied standard safety measures, including a vertical laminar air-flow cabinet and latex gloves. The amount of platinum-containing drugs prepared for intravenous application ranged from 40-3260 mg/day. Urinary platinum was detected in 9 of 52 urine samples collected on days when platinum-containing drugs were mixed (limit of determination 4 ng/l). In comparison with a non-exposed control group, elevated urinary platinum levels were found in one pharmacist (35 ng/g creatinine) and one pharmacy technician (28 ng/g creatinine). The pharmacist's urinary platinum remained elevated after 2 days without occupational exposure to antineoplastic drugs. The urinary platinum level of the pharmacy technician dropped considerably after several weeks without handling cytostatic drugs. Voltammetric detection of urinary platinum is a highly sensitive method suitable for biological and environmental monitoring.

Adult↗

Factor analysis of essential and toxic elements in human placentas from deliveries in arctic and subarctic areas of Russia and Norway.

Concentrations in human placenta of 11 essential elements (P, Ca, Mg, Cu, S, Na, Fe, Zn, K, Se, Mn) and 5 toxic elements (Ba, Sr, Pb, Ni, Cd) are compared for each of two arctic communities in eastern Norway and western Russia, and for another in each country located at more southerly latitudes. All but Mg, Fe, P and K were present in higher concentrations in the Russian study group. The observed inter-element correlations are reflected by the four major factors identified in a principal component analysis. The total variation explained was 67.3%, of which more than half (35.3%) was contributed by Factor 1. P, Ca, Mg, Ba, Sr, Pb, and Ni were major contributors to this factor. The placental concentrations of these elements depended strongly on gestational age, increasing from about week 35 and peaking near weeks 39 and 40, and exhibited skewed frequency distributions and a dependence on maternal smoking. The gestational-dependent mineralization of the placenta is interpreted to reflect the deposition of metal phosphates coinciding with smoking-induced tissue damage. The loadings of the remaining three factors are reviewed in the context of common uptake mechanisms, similar biochemistries and unique transport pathways. The inter-element relationships and grouping of the elements observed should constitute a scientific base for the use of placenta composition in environmental monitoring and epidemiological studies.

Adult↗

[Polarization fluorescence immunoanalysis of herbicides of the S-1,3,5-triazine class].

Polarization fluoroimmunoassay for s-triazine herbicides was developed. The sensitive of assay is higher with using shortest chemical "bridge" length between the molecule of antigen and fluorescent label. The detection limit of atrazine in 50 microL sample was 6 ng/ml. The total time required for an assay of 10 samples was 6 ng/ml. The total time required for an assay of 10 samples is approximately 7 min. The method may be used for environmental monitoring of s-triazine herbicides in water samples.

Environmental Monitoring↗

Sewer sediment transport studies using an environmentally controlled annular flume.

This paper provides an overview and some preliminary results of a collaborative project recently completed at WL Delft Hydraulics. It describes tests in an annular flume, in which sediment deposits were formed under carefully controlled and monitored environmental conditions. The deposits were then subjected to a series of time steps in which the rotational speed of the flume's top and bottom plates was increased, progressively increasing the bed shear stress. The sediment deposits were formed using three different types of sediment. An artificial organic sediment, together with a uniformly sized sand were selected as surrogate sewer sediments. The deposits in the remaining experiments were real in-sewer sediments, from catchments in the UK (Dundee) and The Netherlands (Loenen). During the erosion test, total and volatile suspended solids concentration, particle size distribution of the eroded sediment, and COD and DO levels were recorded. The bed surface topography was also measured so that the influence of the deposit formation condition on bedforms could also be examined. Where bed consolidation times were least 24 hours a biologically active surficial layer was observed to develop at the sediment/water interface. The initial deposit conditions (temperature and deposit duration) were both found to have a significant impact on the subsequent erosion of the deposit.

Environmental Monitoring↗

Bird skins in museum collections: are they suitable as indicators of environmental metal load after conservation procedures?

To find out whether modern conservation treatments alter the level of metals in feathers, we analysed the content of 10 metals in feathers before and after skins were washed with detergent and treated with Eulan U-33 (a commonly used preservative at museums). Feathers of 31 birds of Goshawk Accipiter gentilis, Eagle Owl Bubo bubo. Feral Pigeon Columba livia domest, and Common Eider Somateria mollissima were analysed. We found that in most cases metals were partly washed out of the feathers, but the effects were related to species and type of feather. The value of bird skins as indicators of environmental metal load is therefore affected by this treatment. It is recommended that the conservation techniques used at museums should be reconsidered if skins are intended for specimen banking for future reference in environmental monitoring schemes and research.

Animals↗

Urinary excretion of mutagens, thioethers and D-glucaric acid in workers exposed to bitumen fumes.

The authors carried out biological monitoring of the mutagenic/carcinogenic hazards associated with exposure to bitumen fumes during paving operations, analysing some biological parameters in the urine of a group of exposed workers. The urine samples were studied for mutagenicity by the Ames test and for thioethers concentration. D-Glucaric acid urine excretion was also determined to investigate the enzymatic induction potential of bitumens. Even though, in a previous environmental monitoring phase, a low content of mutagenic/carcinogenic compounds was found in bitumen and air samples, urinary mutagenicity data of exposed workers were statistically higher than those of a group of unexposed subjects. The urinary mutagenicity increased further if exposure to bitumens was associated with cigarette smoking. Thioethers were higher only in subjects exposed simultaneously to bitumens and cigarettes. D-Glucaric acid excretion did not increase significantly. The authors think that this type of coupled environmental and biological monitoring is a valid tool for a better evaluation of the mutagenic/carcinogenic exposure to bitumens or similar complex mixtures.

Air Pollutants, Occupational↗