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Results for “DENTAL PULP”

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At least 667 records · Page 37Linked to original sources
PubMed1985

Immunohistochemical demonstration of the nerves in human dental pulp with antisera against neurofilament protein and glia-specific S-100 protein.

Human dental pulp was investigated by an immunofluorescence method using antisera against neurofilament protein (NFP) and glia-specific S-100 protein. Nerve fibers coursing through the pulp were selectively stained with the anti-NFP serum, and their Schwann sheath with the S-100 antiserum. In addition to a definitive demonstration of the subodontoblastic nerve plexus, we found another hitherto unknown nerve plexus composed of delicate fibers at the base of the odontoblastic layer. This study shows that immunohisto chemical techniques using antisera against nervous system-specific proteins are superior to previous silver impregnation methods, due to their specificity and constancy in reaction.

Dental Pulp↗
PubMed1995

Localization of chondroitin sulphate and dermatan sulphate in human dental pulps--an immunohistochemical study.

The distribution of dermatan sulphate and chondroitin sulphate in human dental pulps has been assessed using monoclonal antibodies and immunoperoxidase localization techniques. The pulpal tissues were reacted with specific antibodies following pretreatment of the sections with chondroitinase ACII or chondroitinase ABC. Both the 4- and 6-sulphates isomers of chondroitin sulphate were detected in the tissues studied. Very little dermatan sulphate could be detected. These glycosaminoglycans appeared throughout the pulpal connective tissues with a particularly strong localization to the region adjacent to the odontoblastic and predentine layers. Such distribution strongly implicates chondroitin sulphate in the mineralization process of human dentine.

Chondroitin Sulfates↗
PubMed1990

[Dental pulp evoked potentials].

The purpose of this work was to explore the possibilities of dental pulp evoked potential registration and to standardize the mean values of peak latencies and amplitudes recorded from the vertex in both sexes. The long latency evoked potentials of the brain cortex were obtained by electrostimulation with the corresponding parameters. The peak latencies recorded in the female group N 40, P 80 and N 140 were significantly shorter than peak latencies N 50, P 90 and N 150 in the male group. Peak amplitudes recorded in the female group were higher than in the male group, but statistically they were not significant.

Adult↗
PubMed1997

In vitro culture of human dental pulp cells: some aspects of cells emerging early from the explant.

Cells emerging from a dental pulp explant were studied in order to elucidate the origin of precursor cells implicated in the formation of reparative dentin in vivo. Such cells observed at the very early stage of culture (days 5-10) were different from fibroblast-like cells obtained after one subculture. These early cells were round or elongated, with thin spinous processes, highly mobile, and contained numerous lipid vesicles. Incorporation of 1-[14C]palmitic acid did not show an increased incorporation of radiolabeled total lipids or triglycerides into these early cells compared to cells after four subcultures or control skin fibroblasts, suggesting that the lipids in vesicles were not synthesized by the cells. Since these cells also show a high level of fluid phase uptake via macropinocytosis, it is suggested that these lipids originate from macropinocytosis. Between days 10 and 20, these cells spontaneously start conversion into cells that have a fibroblast-like morphology, are less mobile, and lack lipid vesicles. Their morphology, movement, and macropinocytosis suggest that these cells, which migrate from the pulp explants, are mesenchymal cells related to mononuclear phagocytes/histiocytes.

Adolescent↗
PubMed1998

Effect of inflammation on the delivery of drugs to dental pulp.

Although numerous studies have evaluated the effects of drugs on inflammation, comparatively few studies have evaluated the effects of inflammation on drugs. In this study, we have evaluated whether pulpal inflammation alters the delivery of flurbiprofen or Evan's blue, two agents that bind with high affinity to plasma proteins. The results indicate that pulpal inflammation alters the delivery of these agents to inflamed molars, that activation of capsaicin-sensitive nerves increases pulpal content of protein-bound agents, and that reduced pH increases free drug concentrations of flurbiprofen. Thus, alterations in both plasma extravasation and tissue pH seem to be relevant factors regulating the delivery and bioavailability of this nonsteroidal anti-inflammatory drug to dental pulp. Because many drugs used in endodontics (e.g. nonsteroidal anti-inflammatory drugs, clindamycin, bupivacaine, etc.) are heavily bound to plasma proteins, it is likely that the status of pulpal inflammation is a contributing factor in modifying the pharmacological efficacy of these agents.

Animals↗
PubMed1985

Effects of vitamin E dietary supplements on the exposed dental pulp in rats.

Forty adult male Sprague-Dawley rats were divided into two equal groups, control (Group 1) and experimental (Group 2). Group 2 received 100 mg of dl-alpha-tocopherol acetate (vitamin E) twice weekly by the oral route. After 2 weeks the animals in both groups were subjected to pulp exposures that were capped with zinc oxide and eugenol and to pulp exposures that were left open. The oral administration of 100 mg dl-alpha-tocopherol acetate in the Group 2 animals was continued for periods of 1 day to 4 weeks after the operative procedures. The animals were killed 1 day, 3 days, and 1, 2, and 4 weeks after the procedures. The most severe, prolonged, and extensive pulpal disease was seen in both groups when the pulp exposure was left open to the oral flora. The most favorable repair and healing results took place after 1, 2, and 4 weeks in the Group 2 animals in pulp exposures covered with zinc oxide and eugenol.

Animals↗
PubMed2004

[Investigation of primary cell culture for dental pulp: comparing of coronal pulp and root pulp].

PURPOSE: To compare and establish an ideal method for the culture of human primary dental coronal and root pulp cells. METHODS: The whole dental pulp tissues were obtained from healthy and young human teeth extracted for orthodontic purposes. The cells in coronal and root pulp were separately cultured by the tissue explant and tissue explant-collagenase digestion(0.1% type I collagenase) methods. By evaluating the attachment efficiency,viability and the successfulness of the cultured cells, the optimal primary cell culture process of human dental coronal and root pulp was determined. RESULTS: The tissue explant culture had more successfulness,more viability and less attachment efficiency than that of tissue explant-collagenase digestion method in the culture of human coronal and root dental pulp cells. CONCLUSIONS: Tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells. The ideal method for dental coronal and root pulp cells was established in this study.

Adolescent↗
PubMed1993

Characterization of a system of mineralized-tissue formation by rat dental pulp cells in culture.

Pulp tissue was obtained from maxillary incisors of young adult male Wistar rats, minced and digested with 0.5% trypsin and 0.02% EGTA at 37 degrees C for 30 min. Dissociated cells were cultured with or without 10 nM dexamethasone using Eagle's minimal essential medium supplemented with 10% fetal bovine serum and 50 micrograms/ml ascorbic acid. Confluent cells were subcultured at 7 days and the medium further supplemented with beta-glycerophosphate (beta-GP). Dexamethasone in primary culture and/or secondary culture enhanced the formation of mineralized tissue while > 5 mM beta-GP was necessary for mineralization to occur. Biochemical analysis of the radiolabelled medium revealed that these cells produced type I, type I trimer and type III collagens. Analysis of [32PO4]-labelled medium, using DEAE-Sephacel ion-exchange chromatography and sodium dodecylsulphate-polyacrylamide gel electrophoresis, showed that these cells produced phosphophoryn-like protein. These results indicate that some of the rat dental pulp cells in culture express an odontoblast-like phenotype.

Animals↗
PubMed1999

Apoptosis of dental pulp cells and their elimination by macrophages and MHC class II-expressing dendritic cells.

Apoptosis of dental pulp cells of rat incisors was investigated by the TUNEL method and electron microscopy. The results showed that a considerable amount of apoptosis occurred in the pulp, increasing in extent with incisal direction. OX6, ED1, and ED2 antibodies were used to detect macrophages and dendritic cells in combination with immunoelectron microscopy. Apoptotic fragments were eliminated mainly by MHC Class II-expressing cells, including dendritic cells positive for the OX6 antibody, and by MHC Class II-negative macrophages. Macrophages and dendritic cells positive for OX6, ED1, or ED2 increased from the apical to incisal direction of the incisor. These results indicate that apoptosis contributes to normal pulp formation and maintenance.

Animals↗