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Prevalence and diversity of Bartonella in rodents of northern Thailand: a comparison with Bartonella in rodents from southern China.

We report results of the first study to investigate the distribution and diversity of Bartonella in rodents from Thailand. Whole blood from 195 rodents, representing six species, was tested for the presence of Bartonella species using standard culture techniques. Isolates were obtained from 17 (8.7%) of the samples, and 14 of those isolates represented distinct strains, based upon partial sequencing of the citrate synthase (gltA) gene. Phylogenetic analysis of the isolates and other Bartonella species indicated that five unique isolates from Bandicota indica form a cluster that may represent a new Bartonella species. Two additional isolates from B. indica clustered together, and were nearly identical to an isolate from Apodemus draco collected in southern China. Importantly, a number of the isolates from Thailand rodents are closely related to B. grahamii and B. elizabethae, species which have been associated with human illness.

Animals↗

[Bacteria of Bartonella genus: characteristics, pathogenicity factors and methods of molecular-genetic investigation].

Bartonella bacteria are an agent of a variety of human and animal diseases whose etiology used to be unknown. The survey contains a description of the above bacteria with the established pathogenicity factors and with the molecular and genetic approaches to studies of genes' functions and of their regulation being in the focus of attention. Modern methods related with the genus-specific and species-specific determination and identification of Bartonella bacteria are discussed.

Animals↗

Prevalence of human pathogens in cat and dog fleas in New Zealand.

AIMS: To provide further information on the prevalence of Rickettsia felis, Bartonella hensela, and B. clarridgeiae in cat and dog fleas in New Zealand and their distribution in the country. METHODS: We used PCR and sequencing with primers for the its and pap 31 (for Bartonella spp.), and the gltA and OmpB (for Rickettsia spp.) genes on DNA from fleas collected from dogs and cats presenting to 3 widely separated veterinary practices on the North Island. RESULTS: DNA of R. felis (19%), B. henselae (11%), and B. clarridgeiae (7%) was found in the 114 cat fleas (Ctenocephalides felis) we studied. The DNA of both B. henselae and B. clarridgeiae was found in 3 fleas (from 2 animals); B. clarridgeiae and R. felis in 1 flea; B. henselae and R. felis in 5 fleas (from 3 animals); and R. felis, B. henselae, and B. clarridgeiae in 2 fleas (from 1 animal). No amplicons were obtained from 3 dog fleas (Ctenocephalides canis). CONCLUSIONS: The emerging human pathogens, R. felis, B. henselae, and B. clarridgeiae, are prevalent and widely distributed in cat fleas in the North Island of New Zealand.

Animals↗

Antibodies to Bartonella vinsonii subsp. Berkhoffii in Moroccan dogs.

Bartonella vinsonii subsp. berkhoffii is a fastidious microorganism that has been recognized as an emerging human and canine pathogen. We report for the first time on the prevalence of antibodies to B. vinsonii subsp. berkhoffii in domestic dogs from Morocco. The overall seroprevalence was 38% (56 of 147 dogs tested). Most of the seropositive dogs were stray dogs from Rabat (36%, 8 of 22) and Khenifra (47%, 47 of 101). Antibodies against B. vinsonii subsp. berkhoffii were found infrequently among pet dogs from Rabat (4%, 1 of 24).

Animals↗

Bartonella and Rickettsia in fleas and lice from mammals in South Carolina, U.S.A.

Species in the genera Bartonella and Rickettsia are vector-borne pathogens of humans and domestic animals. The natural reservoirs and enzootic transmission cycles of these bacteria are poorly known in South Carolina. Thirteen species of lice and fleas were collected from urban animals and screened for the presence of Bartonella and Rickettsia by PCR amplification using genus-specific primers. Bartonella henselae was present in cat fleas (Ctenocephalides felis) from Virginia opossums (Didelphis virginiana) and a novel genotype of Bartonella was detected in Orchopeas howardi from an eastern gray squirrel (Sciurus carolinensis). We detected R. typhi and three novel genotypes Rickettsia in other species of fleas and lice. Rickettsia typhi, the causative agent of murine typhus, was detected in two pools of lice (Enderleinellus marmotae) from the woodchuck (Marmota monax). Cat fleas harbored one of two novel genotypes of Rickettsia. A third novel Rickettsia was detected in Orchopeas howardi from an eastern gray squirrel.

Animals↗

[Fleas and diseases transmissible to man].

Fleas are wingless insects that parasitize mammals and more rarely birds. They are able to jump and may bite people. Adult fleas are hematophagous. The impact of fleas on public health is related to their ability to act as vectors for transmission of infectious agents during blood meals. The purpose of this article is to describe fleas and the diseases that they transmit to humans. Special focus is placed on epidemiological aspects.

Animals↗

Bartonellosis. An immunodepressive disease and the life of Daniel Alcides Carrión.

The history of human Bartonellosis, a unique South American biphasic bacterial disease, is reviewed. The course of this disease and its natural history offer many puzzling problems. Its geographic distribution, its absence of a natural reservoir, the development of transient immunosuppression during the acute hematic phase, and the subsequent development of vascular proliferations are highlighted. These vascular proliferations represent a unique example of tissue reactivity recalling the vascular proliferations of patients with AIDS. In addition, the authors review the life and times of Daniel Alcides Carrión, a Peruvian medical student who in 1885 by self-experimentation linked both phases of the disease and died in doing so. Carrión's life, and the impact of his experiment on the subsequent study of Bartonellosis by Peruvian and international scholars, epitomize the constant struggle of the Peruvian medical profession (past and present) to upgrade domestic biomedical research and to find a national identity.

Bartonella Infections↗