Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Uchida Method”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 649 records · Page 36Linked to original sources
PubMed1994

[The effect of preclotting and collagen coating on endothelializing rate and thrombogenesity of Dacron grafts in the canine thoracic aorta].

Although high porosity knitted Dacron is generally recognized to have superior healing characteristics over woven Dacron, its porosity must be controlled at the clinical operation. This can be achieved with several materials, including geratin, insoluble collagen, albumin, and fibrin. We made atherocollagen coated graft using EX-313 as a new crosslinking agent. The purpose of this study is to compare the endothelializing rate and thrombogenesity of Dacron grafts coated by atherocollagen in the canine thoracic aorta with preclotting grafts with blood or albumin. Five groups were studied: Control group (n = 10), without preclotting; A-P group (n = 8), preclotting with albumin; B-P group (n = 5), preclotting with blood; W-C group (n = 5), atherocollagen coating with low cross-linkage; S-C group (n = 7), atherocollage coating with high cross-linkage. Thoracic aorta was replaced with 8 mm graft in length of 5.0 to 5.5 cm using temporary bypass with anthron tube. Grafts were harvested 3 months following implantation, and the endothelized surface ratio was calculated by microscopic line sampling method. Endothelized surface ratio of Control group, A-P group B-P group, W-C group and S-C group were 85%, 55%, 67%, 93% and 85%, respectively. Endothelized surface ratio of W-C group and S-C group were higher (p < 0.05) than those of A-P group, B-P group. There were thrombus in non-epithelized area. We conclude that atherocollagen coated graft had superior antithrombogenesity compared to albumin or blood preclotting graft.

Animals↗
PubMed1994

[Experimental evaluation of combination chemotherapy with UFT and cisplatin for lung cancer].

Combined treatment of UFT with cisplatin (CDDP) achieved remarkable elongation of survival time of BDF1 mice, which were intravenously transplanted with Lewis lung carcinoma (LLC). Three cycles of weekly iv administration of CDDP and vindesine (VDS), and daily oral administration of UFT were scheduled. Combination UFT (18 mg/kg/day) with CDDP (6.0 mg/kg/day) yielded tumor-free survivors in all of the treated animals with slight body weight loss. Its efficacy was superior to that of the combination of CDDP with VDS. Supra-additive cytotoxic activity against LLC cells was observed by Isobologram method on the schedule of the exposure to 5-fluorouracil preceding the exposure to CDDP. Additive effect was observed by the reversed schedule of the treatment. Using human non-small-cell lung cancer (NSCLC) cell lines which derived from histologically different type, additive or greater additive sensitivity to both of the sequential treatment schedules with 5-FU and CDDP was observed. These results suggest that the combination chemotherapy of UFT with CDDP for lung cancer may show high antitumor efficacy with low toxicity in a clinical setting.

Animals↗
PubMed1996

The activation of K-ras gene at an early stage of lung tumorigenesis in mice.

To clarify the exact timing of K-ras gene mutational activation in lung tumorigenesis of mice, we applied a sensitive mutant allele specific amplification (MASA) method to pulmonary DNA from urethane-treated mice. The activation of K-ras gene with 61st codon AT mutation was detected in the lungs of mice at day 14 but not day 7 after urethane treatment by MASA. The mutation of MASA products was also checked by XbaI restriction fragment length polymorphism analysis and DNA sequencing. These data suggest that the mutation of K-ras gene in the lungs of mice treated with urethane occurred at the early stage of lung tumorigenesis.

Animals↗
PubMed2002

SPARC stimulates the synthesis of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells.

BACKGROUND: Osteonectin/secreted protein, acidic and rich in cysteine (SPARC) is expressed in periodontal ligaments. Therefore, a better understanding of the action of SPARC on periodontal ligament cells could help to elucidate remodelling and repair mechanisms in periodontal tissue. In the present study, we examined the effects of human platelet-derived SPARC (hp-SPARC) on the expressions of SPARC and osteoprotegerin/osteoclastogenesis inhibitory factor (OPG/OCIF), alkaline phosphatase (ALPase) activity, matrix metalloproteinase-2 (MMP-2) production and DNA synthesis in cultures of human periodontal ligament (HPL) cells. METHODS: HPL cells at the sixth passage were exposed to hp-SPARC. The expression of OPG/OCIF and SPARC mRNAs was examined by Northern blot analysis. The protein levels for OPG/OCIF and MMP-2 were determined by Western blot analysis. ALPase activity was measured by the method of Bessey et al. DNA synthesis was estimated by incorporation of [3H] thymidine. RESULTS: Hp-SPARC enhanced OPG/OCIF synthesis at the protein and mRNA levels. Hp-SPARC also enhanced DNA and MMP-2 synthesis dose-dependently, but had little effect on ALPase activity and SPARC mRNA expression. CONCLUSION: SPARC may play a role in remodelling and repair of periodontal tissue by promoting proliferation and MMP-2 production. It may also regulate osteoclast formation through OPG/OCIF in periodontal ligament cells.

Alkaline Phosphatase↗
PubMed2000

Immunoglobulin G and M hepatitis C virus core antibody (JCC.2) response in chimpanzees infected experimentally with hepatitis C virus.

OBJECTIVE: To evaluate changes in serum immunoglobulin M (IgM) and G (IgG) hepatitis C virus (HCV) core antibodies after HCV infection in acute hepatitis C. METHODS: Serum HCV RNA and IgM and IgG HCV core antibodies were investigated using sera sequentially sampled from three chimpanzees experimentally infected with HCV. Serum IgG HCV core antibody titer was measured using a JCC.2 enzyme-linked immunosorbent assay (ELISA) kit (Chemo-Sera-Therapeutic Research Center, Kumamoto, Japan). IgM core antibody titer was measured using horseradish peroxidase-labeled monoclonal anti-human IgM as the secondary antibody for the JCC.2 ELISA kit. Serum HCV RNA was detected using the 5' noncoding region as the primer according to the reverse transcriptase (RT) nested polymerase chain reaction (PCR) and competitive RT-PCR method. RESULTS: IgM JCC.2 antibody was detected when alanine aminotransferase (ALT) peaked, showing the closest correlation with the changes in ALT. A period during which IgM JCC.2 antibody was positive but HCV RNA as determined by RT-nested PCR was negative was observed after the elevation of ALT level. CONCLUSION: These results indicate the usefulness of detection of serum IgM JCC.2 antibody in making a definitive diagnosis of acute hepatitis C and the follow-up observation of hepatitis C.

Acute Disease↗
PubMed2004

Immunochemical detection of 3-deoxyglucosone in serum.

3-Deoxyglucosone (3-DG) is a metabolite of glucose that is thought to lead to the production of advanced glycation end products in diabetes. The previous assay for 3-DG in serum was based on a multi-step protocol, including derivatization, extraction, HPLC separation, and detection. In the current studies, we established a monoclonal antibody that recognizes the 3-DG-derivative, which is generated by the reaction of 3-DG and a 2,3-diamino-benzene derivative. Attachment of a biotin moiety to the 2,3-diamino-benzene ring via a linker allowed development of a highly sensitive chemiluminescent enzyme immunoassay for 3-DG equivalents. Unlike the previous assay, this method does not require extraction of 3-DG derivatives from serum. Treatment of 3-DG in serum with the DAB-link-biotin produced a quinoxaline derivative, which was specifically recognized by the monoclonal antibody. Using this assay, we found that serum 3-DG was higher in streptozotocin-induced diabetic rats than in normal control rats (25+/-5.6 vs. 9.8+/-1.1 microg/L). This simple assay may allow the monitoring of conditions leading to the accumulation of advanced glycation end products and evaluation of the risk of complications in diabetic patients.

Animals↗
PubMed1994

The distribution of amyloid beta precursor protein in canine brain.

The distribution of amyloid beta precursor protein (APP) in canine brain was investigated. By immunoblot analysis, APP-positive bands corresponding to proteins of 105-120 kilodalton were recognized in all canine brains regardless of the individual age of the dogs. Bands of similar molecular mass were also detected in the meninges, cerebrospinal fluid, and several visceral organs. Immunohistochemical studies were performed using cryostat and paraffin-embedded sections pretreated with formic acid or by the hydrated autoclave method. In the normal canine brain, APP was found to be distributed in the neurons and vascular system. In the brains with SP, obvious accumulation of APP was observed in swollen neurites within amyloid plaques, although the relationship between APP and diffuse plaques was unclear. APP accumulation in swollen axons was also seen around necrotic foci in the brain of one dog with necrotizing purulent encephalitis. These studies revealed that distribution of APP in canine tissues, especially in the brain, and the accumulation of APP in swollen neurites or axons.

Amyloid beta-Protein Precursor↗
PubMed1998

[Multi-parametric analysis for leukemic immunophenotyping].

We have investigated whether immunophenotyping measured by laser flow cytometry could be corresponding to FAB classification of acute leukemias, using multi-parametric analysis. Sixty-one patients with acute leukemias have been evaluated, including 23 patients with acute lymphocytic leukemias (ALL) and 38 patients with acute myelogenous leukemias. In principal component analysis, positive cell surface antigens could be classified into groups along to the cell lineage and differentiation. In discriminant analysis, the sensitivity by immunophenotypic method to FAB subtypes was 75%, whereas the specificity was over 90%. In cluster analysis, patients have been classified into 4 groups, which essentially corresponded to ALL, M1/M2, M3 and M4/M5. Based on those multi-parametric analysis, a new flow chart has been established, resulting that the sensitivity and the specificity was improved to over 90% and 95% respectively. These results suggest that the classification of acute leukemia using the flow chart could be useful tools for diagnosis of subtypes of acute leukemias.

Antigens, Surface↗
PubMed2000

Measurement of technetium-99 in Marshall Islands soil samples by ICP-MS

Extraction techniques for recovery of technetium-99 (99Tc) for Inductively Coupled Plasma Mass Spectrometry (ICP-MS) measurements were evaluated using soil samples collected from the Marshall Islands. The results of three different extraction techniques were compared: (MI) acid leaching of Tc from ashed soil; (M2) acid leaching of Tc from raw dry soil; and (M3) Tc volatilization from ashed soil using a combustion apparatus. Total Tc recoveries varied considerably between the extraction techniques but each method yielded similar analytical results for 99Tc. Applications of these extraction techniques to a series of environmental samples and ICP-MS measurements have yielded first data on the 99Tc content of Marshall Islands soil samples contaminated with close-in radioactive fallout from nuclear weapons testing. The 99Tc activity concentration in the soil samples ranged between 0.1 and 1.1 mBq g(-1) dry weight (dw). The limit of detection for 99Tc by ICP-MS was 0.17 mBq per sample or 0.014 mBq g(-1) dw under standard operating conditions.

Journal Article↗
PubMed2006

Crystal lattice size and stability of type H clathrate hydrates with various large-molecule guest substances.

To gain a better understanding of the effects of guest molecules on the lattice and stability of type H hydrates, we performed powder X-ray diffraction (PXRD) measurements and semiempirical molecular orbital calculations. The unit cell parameters and cohesive energies of various type H hydrates that contain methane (CH4) were analyzed. PXRD measurements indicated that an increase in the large-molecule guest volume caused the unit cell volume to increase. It was also indicated that a large-molecule guest substance caused the a-axis-direction of the unit cell to increase with little decrease in the c-axis direction. Calculations of cohesive energy by means of a semiempirical molecular orbital method indicated that the functional group and configuration of large-molecule guest substances affects the stability of type H hydrates. It was concluded that the icosahedron (5(12)6(8)) cages do not easily increase in length along the c-axis direction when larger guest molecules are used to form the hydrate, but the 5(12)6(8) cage and the layer of dodecahedron (5(12)) cages can easily increase in length along the a-axis direction due to interactions of the guest-host molecule.

Journal Article↗
PubMed2003

Purification, crystallization and preliminary X-ray analysis of a water-soluble chlorophyll protein from Brassica oleracea L. var. acephala (kale).

A water-soluble chlorophyll protein (WSCP) with a chlorophyll a:b ratio of 6:1 from Brassica oleracea L. var. acephala (kale) was purified and crystallized by the hanging-drop vapour-diffusion method using PEG 8000 and zinc acetate as precipitants. The crystal belongs to the hexagonal space group P6(4)22, with unit-cell parameters a = b = 162.2, c = 38.7 A. A native data set was collected to 2.80 A resolution at 293 K using Cu Kalpha radiation from a rotating-anode generator. Preliminary analysis via molecular replacement identified one kale WSCP monomer in the asymmetric unit. The crystal packing showed a tetrameric structure for kale WSCP, as suggested by previous biochemical studies of WSCPs from Brassicaceae plants.

Amino Acid Sequence↗
PubMed1996

[Investigation for VP4 region of coxsackie virus A16 RNA sequence from hand-foot-mouth disease patients at eastern district of Shizuoka prefecture in 1995].

In 1995 an investigation was made for VP4 regions of coxsackie virus A16 (CA16) RNA sequence from hand-foot-mouth disease patients in eastern district of Shizuoka Prefecture. Subjects were seven patients who were diagnosed as hand-foot-mouth disease due to CA16 at the Ohashi Pediatric Clinic in Susono City. Throat swabs of patients were extracted to RNA. Extracted RNA were assayed by reverse transcription polymerase chain reaction that primers corresponded to VP4 resion of enteroviruses. PCR products were marked by dye-deoxy terminator methods and assayed by direct sequence methods. RNA sequences were classified into two types. Type 1 were three cases, and type 2 were four. The homology was 90.8% between type 1 and type 2. All cases of sixty-nine amino acids were the same as prototype strain. We concluded that the two type strains of CA16 were prevalented in eastern district of Shizuoka Prefecture in 1995. It was at the same time and was widely noted in the eastern district.

Amino Acid Sequence↗
PubMed1994

[Effects on hemodynamics and myocardial metabolism of nicardipine and nitroglycerin during aortic cross clamp in dogs with experimentally produced coronary stenosis].

We investigated the effects of hemodynamics and myocardial metabolism of nicardipine (NIC) and nitroglycerin (NTG) in experimentally coronary constricted dogs. Coronary constriction was produced by reducing 50% of the left anterior descending coronary blood flow. After stabilization of hemodynamics, we cross-clamped the descending aorta and administered nicardipine (10 micrograms.kg-1.min-1) or nitroglycerin (10 micrograms.kg-1.min-1). The drugs were infused for 15 minutes and were discontinued just before the clamp on the aorta was released. In NIC group, the cardiac output (CO) increased 32% and the heart rate (HR) decreased 17% significantly, compared with pre-clamping values. The myocardial blood flow (MBF) in ischemic area measured by hydrogen clearance method was maintained. The value of the myocardial oxygen extraction ratio (MO2EX) decreased significantly for 24.3% compared with the pre-clamping value of 41.4%. On the other hand, in NTG group, CO, HR, and MO2EX were unchanged, but MBF in ischemic area was well maintained. The results of these experimentally coronary constricted dogs suggest that both NIC and NTG maintain the cardiac function during the aortic cross-clamping, but regarding the improvement of the myocardial metabolism, NIC appears to be the better choice for blood pressure control.

Animals↗
PubMed2003

Abnormal bile flow in patients with achalasia.

BACKGROUND: It has been reported that esophageal achalasia is frequently associated with the dysmotility of other digestive organs. However, the prevalence of extraesophageal complications in patients with achalasia still remains poorly understood. We performed cholescintigraphy, using (99m)Tc-pyridoxyl-5-methyl-tryptophan, in patients with esophageal achalasia to assess any possible dysfunction of the sphincter of Oddi associated with achalasia. METHODS: Eight patients (two men and six women) were examined to determine the time required for bile to flow from the bile duct to the duodenum. RESULTS: Excretion time of bile was markedly prolonged in five of the eight patients with achalasia. Scintigraphic findings were not correlated with the radiographic classification of achalasia or with the grading of achalasia. CONCLUSIONS: The present results suggest that a considerable number of patients with achalasia have dysfunction of the sphincter of Oddi, irrespective of the morphological type of achalasia and the grade of esophageal dilatation.

Adult↗
PubMed2006

Retroperitoneoscopic partial nephrectomy using radiofrequency ablation.

Two patients with renal tumors underwent retroperitoneoscopic partial nephrectomy. The renal tumors were initially treated with radiofrequency ablation. This method allowed tumor excision to be achieved without clamping the renal pedicle. Residual renal function was well maintained as determined by enhanced computed tomography scanning and measurement of the serum creatinine level. There were no complications such as vascular damage or collecting system injury. The pathological diagnosis was clear cell carcinoma (pT1) in Patient 1 and was not determined in Patient 2 because of entire ablation. No recurrence has been observed after 3 years and 2 years of follow up, respectively. Radiofrequency ablation was useful for control of local bleeding during retroperitonaoscopic partial nephrectomy.

Adenocarcinoma, Clear Cell↗
PubMed1995

Infrequent involvement of mutations on neurofibromatosis type 1, H-ras, K-ras and N-ras in urothelial tumors.

The neurofibromatosis type 1 (NF1) gene is considered a tumor-suppressor gene whose product acts upstream of ras. The ras gene is an oncogene very commonly detected in human cancers and consists of three families, H-ras, K-ras and N-ras. These genes are converted to active oncogenes by point mutations in codon 12, 13, or 61. Examination was made of the mutations of these genes in 39 urothelial malignant tumors (31 bladder cancer, 6 renal pelvic tumor, and 2 ureter tumors) using polymerase chain reaction single-stranded conformation polymorphism and direct sequencing methods. Three of 39 (7.7%) cases showed mobility shifts in the ras family gene but no point mutations in NF1 and N-ras genes could be detected. Mutations were found in 1 case in H-ras at codon 13 (GGT-GTT/GGT) and K-ras at codon 12 in 2 cases (GGT-GCT/GGT, GGT-GTT/GGT). All 3 cases had progressed far beyond grade 2 and stage pT2. It follows from the above that NF1 and ras gene mutations are infrequent in the pathogenesis of urothelial tumors.

Base Sequence↗
PubMed1993

Application of the bromophenol blue (BPB) staining method to rat fetal cartilage previously stained with alizarin red S.

The application of a convenient bromophenol blue (BPB) cartilage staining method on rat fetal skeleton, previously stained with alizarin red S, was investigated. This staining method made it possible to observe uncertain cartilage anomalies and unossified caudal vertebrae, which were poorly detected by single staining with alizarin red S. Namely, the anomaly of chondral rib bifurcation was detected and the number of unossified caudal vertebrae in rat fetuses was demonstrated as being identical with adult rat caudal vertebrae. The BPB staining has the notice to make the pH 4 adjusted specimens and has the advantage of fading with water or ethanol at pH 8 without discoloration of alizarin red S on the bones.

Animals↗
PubMed1995

Distribution of GABA-immunoreactive premotor neurons projecting to the trigeminal motor nucleus in the rat.

The distribution of neuronal cell bodies with gamma-aminobutylic acid (GABA)-like immunoreactivity projecting to the trigeminal motor nucleus were examined in the rat brainstem by the retrograde transport of horseradish peroxidase (HRP) and immunofluorescence methods. After HRP injection into the unilateral trigeminal motor nucleus, retrogradely labeled neurons which showed GABA-like immunoreactivity were observed throughout the parvocellular reticular nucleus in the pons with ipsilateral predominance. A few neurons were encountered in the contralateral supratrigeminal nucleus, ipsilateral caudal pontine reticular nucleus. All labeled neuronal cell bodies were small or of medium size; no large cells were labeled. The results indicated that cell bodies of GABAergic premotor neurons projecting to the trigeminal motoneurons were mainly located in the bilateral parvocellular reticular nucleus of the pons.

Animals↗