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At least 649 records · Page 36Linked to original sources
PubMed2005

How we process trephine biopsy specimens: epoxy resin embedded bone marrow biopsies.

Improved cytomorphology of semithin resin sections over paraffin wax embedded sections may be important in diagnostic haematopathology. However, resin embedding can make immunohistochemical antigen detection or DNA isolation for clonal gene rearrangement assays difficult. This review describes the processing of bone marrow biopsies using buffered formaldehyde based fixation and epoxy resin embedding, with or without EDTA decalcification. Traditional semithin resin sections are completely rehydrated after etching in home made sodium methoxide solution. Resin elimination allows high resolution staining of tissue components with common histological stains. Efficient antigen retrieval and the Envision-HRP system permit the immunohistological detection of many antigens of diagnostic relevance, with retention of high quality cytomorphology. Furthermore, DNA can be extracted for clonality analysis. The technique can be completed within a similar time period to that of paraffin wax processing with only approximately 30% increase in cost. This technique has been used for diagnosis in over 4000 bone marrow biopsies over the past 14 years. By meeting traditional and contemporary demands on the haematopathologist, it offers a powerful alternative to paraffin wax processing for diagnosis and research.

Antigens↗
PubMed2006

Splitting bone marrow trephines into frozen and fixed fragments allows parallel histological and molecular detection of B cell malignant infiltrates.

Clonality analysis of the immunoglobulin heavy chain (IgH) gene is helpful in identifying malignant B cell infiltrates in the bone marrow and is usually carried out on separate aspirates or on the same formalin-fixed decalcified bone marrow specimen. To determine whether the removal of the decalcification step would improve the molecular analysis, we first studied 12 bone marrow specimens with lymphoma infiltration split into a fixed and a small frozen fragment. Both the detection rate of IgH gene monoclonality and DNA quality were found to be superior in the frozen part than in the fixed part. Conversely, to evaluate whether the split would compromise histological analysis, we selected a series of 134 bone marrow specimens obtained from patients with small B cell lymphoma and showing IgH monoclonality on the frozen part. The histological detection rate of infiltrated or suspicious infiltrates (95%) on the fixed part was not altered by saving a frozen part.

Biopsy↗
PubMed1991

Routine histological compared with immunohistological examination of bone marrow trephine biopsy specimens in disseminated neuroblastoma.

The ability to detect infiltration in bone marrow biopsy specimens from patients with disseminated neuroblastoma was assessed by immunohistological and routine histological methods. Frozen cores from 33 staging procedures were tested with UJ13A and UJ127.11. Immunopositive tumour cells were found in 10 of 17 staging procedures in which tumour was detectable by routine histological methods. Positive cells or stromal material were also found in eight of 12 staging procedures in which distorted architecture and fibrosis, but no obvious tumour, had been noted. Paraffin wax embedded cores from 29 of the same staging procedures were tested with antibodies against neurone specific enolase and neurofilament. Only a single core reacted with anti-neurofilament antibody. Neurone specific enolase positive cells or stromal material were found in nine of 15 staging procedures in which obvious tumour was detectable. Although these immunohistochemical techniques proved inferior to routine histology in their ability to detect obvious tumour, the demonstration of immunopositive stromal tissue which was not frankly malignant supports the view that distorted, fibrotic marrow may reflect persistence of neuroectodermal tissue and justifies its distinction from normal marrow when reporting the response to treatment.

Adolescent↗
PubMed2002

Sigmoidoscopy-assisted colostomy--an adapted trephine stoma formation.

BACKGROUND/AIM: The purpose of this article was to report an adapted colostomy procedure that can be used on patients with multiple morbidities requiring fecal diversion. METHODS: An adapted colostomy was performed on a patient needing fecal diversion who had multiple surgical and medical complications. RESULTS: Thirteen weeks after colostomy placement, the patient's colostomy functioned well and was viable throughout his course without related complications. CONCLUSION: The method of sigmoidoscopy-assisted colostomy described here can be beneficial to the complicated patient needing fecal diversion but unable to tolerate a laparotomy or laparoscopy.

Aged↗
PubMed1978

[Tectonic keratoplasty in the case of infected Elliot's trephination].

A case is reported with respect to the method concerning the treatment of late infection of the filtering blebs after anti-glaucoma surgical procedures. The infected conjunctival flap and the adjoining scleral tissue was excised with the help of a tectonic keratoplasty and the defect was made watertight in the region of the cornea with a running suture and in the region of the sclera with single sutures. In the case reported the tectonic keratoplasty healed up without any complications. The intraocular pressure remained normal. The indication leading to this operation after a late infection of glaucoma filtering blebs is especially reserved for cases in which the conjunctival flap is unmoveable.

Aged↗
PubMed1984

Long-term observations after trephination with scleral flap in glaucoma with threatened point of fixation.

Follow-up examinations 2 months to 9 years after a filtering operation with scleral flap with successful pressure regulation could be carried out in 26 patients with 29 eyes in which a threat to the point of fixation due to glaucoma was present. In the majority of the cases, it was possible to stabilize visual acuity and visual field by the operation. In the remaining cases, there was a slow melting away of the residual visual field without correlation with lens or macular changes or underlying internal conditions.

Adult↗